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- PDB-9s90: Crystal structure of the BRL3 ectodomain from Arabidopsis thalian... -

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Basic information

Entry
Database: PDB / ID: 9s90
TitleCrystal structure of the BRL3 ectodomain from Arabidopsis thaliana in complex with castasterone.
ComponentsReceptor-like protein kinase BRI1-like 3
KeywordsMEMBRANE PROTEIN / receptor kinase / steroid receptor / brassinosteroids / leucine-rich repeat
Function / homology
Function and homology information


steroid binding / transmembrane receptor protein tyrosine kinase activity / receptor protein-tyrosine kinase / non-specific serine/threonine protein kinase / protein serine kinase activity / protein serine/threonine kinase activity / ATP binding / plasma membrane
Similarity search - Function
: / Brassinosteroid receptor BRI1, island domain / Brassinosteroid receptor island domain / Leucine-rich repeat-containing N-terminal, plant-type / Leucine rich repeat N-terminal domain / Leucine Rich repeat / Leucine Rich Repeat / Leucine rich repeat / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily ...: / Brassinosteroid receptor BRI1, island domain / Brassinosteroid receptor island domain / Leucine-rich repeat-containing N-terminal, plant-type / Leucine rich repeat N-terminal domain / Leucine Rich repeat / Leucine Rich Repeat / Leucine rich repeat / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily / Leucine-rich repeat / Leucine-rich repeat domain superfamily / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
: / ACETATE ION / Receptor-like protein kinase BRI1-like 3
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.61 Å
AuthorsCaregnato, A. / Hothorn, M.
Funding support Switzerland, 1items
OrganizationGrant numberCountry
Swiss National Science Foundation310030_205201 Switzerland
CitationJournal: To Be Published
Title: A mechanistic framework for the recognition of chemically diverse brassinosteroids by BRI1-family receptor kinases.
Authors: Caregnato, A. / Chen, H. / Kvasnica, M. / Hohmann, U. / Oklestkova, J. / Ferrer, K. / Broger, L. / Hothorn, L.A. / Strnad, M. / Hothorn, M.
History
DepositionAug 5, 2025Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 3, 2025Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Receptor-like protein kinase BRI1-like 3
B: Receptor-like protein kinase BRI1-like 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)180,06832
Polymers167,2392
Non-polymers12,82930
Water19811
1
A: Receptor-like protein kinase BRI1-like 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,89714
Polymers83,6201
Non-polymers6,27813
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Receptor-like protein kinase BRI1-like 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)90,17118
Polymers83,6201
Non-polymers6,55117
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)62.257, 81.940, 122.476
Angle α, β, γ (deg.)107.022, 91.499, 112.530
Int Tables number1
Space group name H-MP1
Space group name HallP1
Symmetry operation#1: x,y,z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 31 through 204 or resid 206...
d_2ens_1(chain "B" and (resid 31 through 56 or resid 64...

NCS domain segments:

Ens-ID: ens_1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11VALVALLYSLYSAA31 - 20431 - 204
d_12LEULEULEULEUAA206 - 224206 - 224
d_13GLUGLUGLUGLUAA226 - 254226 - 254
d_14LEULEUGLYGLYAA256 - 334256 - 334
d_15ASNASNLEULEUAA336 - 398336 - 398
d_16SERSERSERSERAA400 - 490400 - 490
d_17PROPROSERSERAA492 - 757492 - 757
d_18NAGNAGNAGNAGCC1
d_19NAGNAGNAGNAGCC2
d_110NAGNAGNAGNAGDD1
d_111NAGNAGNAGNAGDD2
d_112NAGNAGNAGNAGAT802
d_113NAGNAGNAGNAGEE1
d_114MANMANMANMANEE4
d_115MANMANMANMANEE7
d_116MANMANMANMANEE5
d_117MANMANMANMANEE6
d_118MANMANMANMANEE10
d_119NAGNAGNAGNAGFF1
d_120NAGNAGNAGNAGFF2
d_121NAGNAGNAGNAGAU803
d_21VALVALTRPTRPBB31 - 5631 - 56
d_22PROPROLYSLYSBB64 - 20464 - 204
d_23LEULEULEULEUBB206 - 224206 - 224
d_24GLUGLUGLUGLUBB226 - 254226 - 254
d_25LEULEUGLYGLYBB256 - 334256 - 334
d_26ASNASNLEULEUBB336 - 398336 - 398
d_27SERSERSERSERBB400 - 490400 - 490
d_28PROPROSERSERBB492 - 757492 - 757
d_29NAGNAGNAGNAGKK1
d_210NAGNAGNAGNAGKK2
d_211NAGNAGNAGNAGLL1
d_212NAGNAGNAGNAGLL2
d_213NAGNAGNAGNAGBZ803
d_214NAGNAGNAGNAGMM1
d_215MANMANMANMANMM10
d_216MANMANMANMANMM7
d_217MANMANMANMANMM8
d_218MANMANMANMANMM5
d_219MANMANMANMANMM6
d_220NAGNAGNAGNAGPP2
d_221NAGNAGNAGNAGQQ1
d_222NAGNAGNAGNAGRR2

NCS oper: (Code: givenMatrix: (-0.461969981298, -0.850969883192, -0.249867953686), (-0.841650613166, 0.331807687198, 0.426060915919), (-0.279656900035, 0.407128869789, -0.869504515024)Vector: 34. ...NCS oper: (Code: given
Matrix: (-0.461969981298, -0.850969883192, -0.249867953686), (-0.841650613166, 0.331807687198, 0.426060915919), (-0.279656900035, 0.407128869789, -0.869504515024)
Vector: 34.8252210212, -7.1493548119, 96.4804163439)

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Receptor-like protein kinase BRI1-like 3 / BRASSINOSTEROID INSENSITIVE 1-like protein 3


Mass: 83619.508 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: BRL3, At3g13380, MRP15.1 / Production host: Trichoplusia ni (cabbage looper)
References: UniProt: Q9LJF3, receptor protein-tyrosine kinase, non-specific serine/threonine protein kinase

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Sugars , 6 types, 21 molecules

#2: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 10
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#3: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1883.668 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-2DManpa1-3[DManpa1-2DManpa1-3[DManpa1-2DManpa1-6]DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,11,10/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-g1_d2-e1_e2-f1_g3-h1_g6-j1_h2-i1_j2-k1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}}}}LINUCSPDB-CARE
#4: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5]/1-1-2/a4-b1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}LINUCSPDB-CARE
#5: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-D-mannopyranose-(1-6)]2-acetamido-2-deoxy- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-D-mannopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4[DManpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1a_1-5]/1-1-2/a4-b1_a6-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-D-Manp]{}}LINUCSPDB-CARE
#6: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1721.527 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-3[DManpa1-2DManpa1-6]DManpa1-6[DManpa1-2DManpa1-3]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,10,9/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-f1_d2-e1_f3-g1_f6-i1_g2-h1_i2-j1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}}}}LINUCSPDB-CARE
#8: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 4 types, 20 molecules

#7: Chemical ChemComp-A1JME / Castasterone / (2~{R},3~{S},5~{S},8~{S},9~{S},10~{R},13~{S},14~{S},17~{R})-17-[(2~{S},3~{R},4~{R},5~{S})-5,6-dimethyl-3,4-bis(oxidanyl)heptan-2-yl]-10,13-dimethyl-2,3-bis(oxidanyl)-1,2,3,4,5,7,8,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-6-one


Mass: 464.678 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C28H48O5
#9: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: SO4
#10: Chemical ChemComp-ACT / ACETATE ION


Mass: 59.044 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C2H3O2
#11: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 11 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.26 Å3/Da / Density % sol: 62.26 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5
Details: 23% w/v PEG 3350, 0.2 M Li2SO4, 0.1 M sodium acetate pH 5.5

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 0.99999 Å
DetectorType: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Dec 4, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.99999 Å / Relative weight: 1
ReflectionResolution: 2.61→49.35 Å / Num. obs: 59210 / % possible obs: 91.7 % / Observed criterion σ(I): -3 / Redundancy: 3.9 % / Biso Wilson estimate: 73.81 Å2 / CC1/2: 1 / Rrim(I) all: 0.185 / Net I/σ(I): 6.5
Reflection shellResolution: 2.61→2.76 Å / Redundancy: 3.9 % / Mean I/σ(I) obs: 1 / Num. unique obs: 9392 / CC1/2: 0.331 / Rrim(I) all: 2.669 / % possible all: 90.2

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Processing

Software
NameVersionClassification
PHENIX1.21.1_5286refinement
XDSdata reduction
XSCALEdata scaling
RefinementMethod to determine structure: FOURIER SYNTHESIS / Resolution: 2.61→49.35 Å / SU ML: 0.5042 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 34.8103
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2576 2936 4.98 %
Rwork0.2233 55990 -
obs0.2251 58926 91.41 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 90.06 Å2
Refinement stepCycle: LAST / Resolution: 2.61→49.35 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10815 0 848 11 11674
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.003111851
X-RAY DIFFRACTIONf_angle_d0.725316119
X-RAY DIFFRACTIONf_chiral_restr0.04272078
X-RAY DIFFRACTIONf_plane_restr0.00461980
X-RAY DIFFRACTIONf_dihedral_angle_d13.08324837
Refine LS restraints NCSType: Torsion NCS / Rms dev position: 4.75284765707 Å
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.61-2.650.41991470.42722527X-RAY DIFFRACTION87.19
2.65-2.690.40061340.40712570X-RAY DIFFRACTION88.02
2.69-2.740.3931320.38852609X-RAY DIFFRACTION89.2
2.74-2.80.39921130.38212523X-RAY DIFFRACTION85.39
2.8-2.850.45131290.39272301X-RAY DIFFRACTION79.23
2.85-2.920.40291380.36322719X-RAY DIFFRACTION93
2.92-2.980.35941500.352754X-RAY DIFFRACTION95.43
2.98-3.060.38061400.33332779X-RAY DIFFRACTION95.3
3.06-3.140.36471520.31732804X-RAY DIFFRACTION95.05
3.14-3.230.33611580.29952722X-RAY DIFFRACTION94.83
3.23-3.340.31751270.30162760X-RAY DIFFRACTION94.28
3.34-3.460.31271540.30032761X-RAY DIFFRACTION93.73
3.46-3.60.26731360.24372719X-RAY DIFFRACTION93.79
3.6-3.760.23791390.21952698X-RAY DIFFRACTION92.2
3.76-3.960.22981450.19972585X-RAY DIFFRACTION89.3
3.96-4.20.22821210.18532378X-RAY DIFFRACTION81.37
4.2-4.530.20591580.16752813X-RAY DIFFRACTION96.49
4.53-4.980.18371370.15522790X-RAY DIFFRACTION96.35
4.98-5.70.23081470.18732819X-RAY DIFFRACTION95.52
5.71-7.180.23981350.19992703X-RAY DIFFRACTION92.87
7.18-49.350.21981440.17122656X-RAY DIFFRACTION91.15
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.115584897272.1349624777-1.216258570656.8997127366-0.151154402814.477369580470.255345069931-0.482262030887-0.5179608978670.887598169551-0.2440723437380.4654456452810.8737465379-0.262069357873-0.0347180449731.104486301230.005023534950610.06814439174640.8487539577860.1621532502760.965918684086-22.3759299813-12.008112222792.6194435256
21.83462810279-0.709190140558-0.8774583964751.629585214070.5569100616631.992023683030.1309104946780.272395108007-0.131292173711-0.334393824489-0.1933727830710.0062508966384-0.0562553177362-0.1566260740420.05755532420040.6378297092460.0408866707574-0.05857197630080.6299516744230.01551488763790.451985145322-18.519939910921.747185991173.3709544241
33.86736362775-1.06968540802-1.729843865622.71803749921.135620461646.00889413680.1246522502760.01793267703510.2010516081970.0691195144886-0.0214138694241-0.0156136775789-0.607938900552-0.214617597421-0.1702363291640.5837585015960.0486392434182-0.05798678390070.6261801906080.07065586970540.443131526138-25.046520941436.768400352194.0822997229
40.8126964463710.08259792011451.732976850162.004156000561.471250216674.935027881210.0891886227707-0.1324138163980.0666937041493-0.144829523609-0.244940773537-0.106236081036-0.381544434426-0.1135200179060.1769275412030.6813419500610.002170236901220.112690507710.6969792999360.07322879669430.587141889458-24.189231628441.197331086113.689196175
54.690158277881.78264561474-1.368685698014.86256156722-1.642490704531.69642964821-0.1819739192510.345364863068-0.488958898649-0.7209971529730.116421778237-0.7879100366870.1396356672960.2380392985610.0745107770990.896193783233-0.131666623950.1321840351340.842348998901-0.09051386902610.82032467232733.941135749147.457429658416.4654942135
63.22210013744-0.699310260799-1.965701233582.947864860480.6193689149022.69840809293-0.292252977934-0.195174526171-0.3105450429130.1843777190350.125621446348-0.246373443010.2550596750680.1359980282390.1612980227690.649222312816-0.0157047154623-0.03685159230980.5420447997810.01520080936480.58122642035312.496755390732.623793727541.1496468
71.57125275846-0.4473356003250.5739987033964.3734524964-0.9982476008911.46822649687-0.229765527858-0.3451123338980.1603316584381.15394215980.136746108557-0.22510033662-0.5085427399310.06866457280220.09134214903670.8685406856790.03162699233570.002898305348910.624953760163-0.07911531417270.505778689127-0.21847320052765.656795503353.3728669815
81.03674584563-0.2735217062471.050805809619.46730648137-2.695694262573.12613478868-0.160648118816-0.02695567613090.03309494511080.4165833082630.389824115620.482604560437-0.289467392085-0.462409329983-0.2419471961510.569937789130.03523771995680.06944458486460.564599968939-0.06118831091720.397536906271-8.4781681291966.194927916136.8546883746
93.109728463470.04439442918291.844363642481.47607034046-0.3085114414572.78354539104-0.0574731361770.2060560987450.0118454167144-0.2071738777740.09338188668330.114419078835-0.177373472925-0.191921278465-0.04009667399010.783285288455-0.02323759024140.1044838345840.649971183450.01554557910450.580443835191-17.904021919375.414426079621.4645614731
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 31 through 153 )AA31 - 1531 - 116
22chain 'A' and (resid 154 through 553 )AA154 - 553117 - 516
33chain 'A' and (resid 554 through 656 )AA554 - 656517 - 619
44chain 'A' and (resid 657 through 757 )AA657 - 757620 - 720
55chain 'B' and (resid 30 through 147 )BB30 - 1471 - 115
66chain 'B' and (resid 148 through 378 )BB148 - 378116 - 346
77chain 'B' and (resid 379 through 553 )BB379 - 553347 - 521
88chain 'B' and (resid 554 through 656 )BB554 - 656522 - 624
99chain 'B' and (resid 657 through 758 )BB657 - 758625 - 726

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