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- PDB-9s9a: Crystal structure of the BRL3 ectodomain from Arabidopsis thalian... -

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Basic information

Entry
Database: PDB / ID: 9s9a
TitleCrystal structure of the BRL3 ectodomain from Arabidopsis thaliana in complex with 6-deoxocastasterone.
ComponentsReceptor-like protein kinase BRI1-like 3
KeywordsMEMBRANE PROTEIN / receptor kinase / steroid receptor / brassinosteroids / leucine-rich repeat
Function / homology
Function and homology information


steroid binding / transmembrane receptor protein tyrosine kinase activity / receptor protein-tyrosine kinase / non-specific serine/threonine protein kinase / protein serine kinase activity / protein serine/threonine kinase activity / ATP binding / plasma membrane
Similarity search - Function
: / Brassinosteroid receptor BRI1, island domain / Brassinosteroid receptor island domain / Leucine-rich repeat-containing N-terminal, plant-type / Leucine rich repeat N-terminal domain / Leucine Rich repeat / Leucine Rich Repeat / Leucine rich repeat / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily ...: / Brassinosteroid receptor BRI1, island domain / Brassinosteroid receptor island domain / Leucine-rich repeat-containing N-terminal, plant-type / Leucine rich repeat N-terminal domain / Leucine Rich repeat / Leucine Rich Repeat / Leucine rich repeat / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily / Leucine-rich repeat / Leucine-rich repeat domain superfamily / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
: / ACETATE ION / Receptor-like protein kinase BRI1-like 3
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 3.2 Å
AuthorsCaregnato, A. / Hothorn, M.
Funding support Switzerland, 1items
OrganizationGrant numberCountry
Swiss National Science Foundation310030_205201 Switzerland
Citation
Journal: To Be Published
Title: A mechanistic framework for the recognition of chemically diverse brassinosteroids by BRI1-family receptor kinases.
Authors: Caregnato, A. / Chen, H. / Kvasnica, M. / Hohmann, U. / Oklestkova, J. / Ferrer, K. / Broger, L. / Hothorn, L.A. / Strnad, M. / Hothorn, M.
#1: Journal: Acta Crystallogr D Struct Biol / Year: 2019
Title: Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix.
Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty ...Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty / Robert D Oeffner / Billy K Poon / Michael G Prisant / Randy J Read / Jane S Richardson / David C Richardson / Massimo D Sammito / Oleg V Sobolev / Duncan H Stockwell / Thomas C Terwilliger / Alexandre G Urzhumtsev / Lizbeth L Videau / Christopher J Williams / Paul D Adams /
Abstract: Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological ...Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological processes and to develop new therapeutics against diseases. The overall structure-solution workflow is similar for these techniques, but nuances exist because the properties of the reduced experimental data are different. Software tools for structure determination should therefore be tailored for each method. Phenix is a comprehensive software package for macromolecular structure determination that handles data from any of these techniques. Tasks performed with Phenix include data-quality assessment, map improvement, model building, the validation/rebuilding/refinement cycle and deposition. Each tool caters to the type of experimental data. The design of Phenix emphasizes the automation of procedures, where possible, to minimize repetitive and time-consuming manual tasks, while default parameters are chosen to encourage best practice. A graphical user interface provides access to many command-line features of Phenix and streamlines the transition between programs, project tracking and re-running of previous tasks.
History
DepositionAug 6, 2025Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 3, 2025Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Receptor-like protein kinase BRI1-like 3
B: Receptor-like protein kinase BRI1-like 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)178,82927
Polymers167,2392
Non-polymers11,59025
Water00
1
A: Receptor-like protein kinase BRI1-like 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,22313
Polymers83,6201
Non-polymers5,60312
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Receptor-like protein kinase BRI1-like 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,60614
Polymers83,6201
Non-polymers5,98713
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)62.163, 82.688, 122.196
Angle α, β, γ (deg.)107.015, 91.353, 112.460
Int Tables number1
Space group name H-MP1
Space group name HallP1
Symmetry operation#1: x,y,z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and ((resid 31 and (name N or name...
d_2ens_1(chain "B" and ((resid 31 and (name N or name...

NCS domain segments:

Ens-ID: ens_1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11VALVALLYSLYSAA31 - 20431 - 204
d_12LEULEULEULEUAA206 - 224206 - 224
d_13GLUGLUASNASNAA226 - 249226 - 249
d_14LYSLYSGLUGLUAA251 - 254251 - 254
d_15LEULEUSERSERAA256 - 324256 - 324
d_16GLYGLYGLYGLYAA326 - 334326 - 334
d_17ASNASNLEULEUAA336 - 398336 - 398
d_18SERSERSERSERAA400 - 490400 - 490
d_19PROPROSERSERAA492 - 757492 - 757
d_1106DC6DC6DC6DCAQ800
d_111NAGNAGNAGNAGAR1096
d_112NAGNAGNAGNAGCC1
d_113NAGNAGNAGNAGCC2
d_114NAGNAGNAGNAGAS1212
d_115NAGNAGNAGNAGAT1227
d_116NAGNAGNAGNAGDD1
d_117MANMANMANMANDD9
d_118MANMANMANMANDD4
d_119MANMANMANMANDD10
d_120MANMANMANMANDD7
d_121MANMANMANMANDD8
d_122MANMANMANMANDD5
d_123MANMANMANMANDD6
d_124NAGNAGNAGNAGEE1
d_125NAGNAGNAGNAGEE2
d_126NAGNAGNAGNAGFF1
d_127NAGNAGNAGNAGFF2
d_128NAGNAGNAGNAGGG1
d_129NAGNAGNAGNAGGG2
d_130NAGNAGNAGNAGHH1
d_131NAGNAGNAGNAGHH2
d_132NAGNAGNAGNAGII1
d_133NAGNAGNAGNAGII2
d_21VALVALTRPTRPBB31 - 5631 - 56
d_22ASPASPLYSLYSBB63 - 20463 - 204
d_23LEULEULEULEUBB206 - 224206 - 224
d_24GLUGLUASNASNBB226 - 249226 - 249
d_25LYSLYSGLUGLUBB251 - 254251 - 254
d_26LEULEUSERSERBB256 - 324256 - 324
d_27GLYGLYGLYGLYBB326 - 334326 - 334
d_28ASNASNLEULEUBB336 - 398336 - 398
d_29SERSERSERSERBB400 - 490400 - 490
d_210PROPROSERSERBB492 - 757492 - 757
d_2116DC6DC6DC6DCBV800
d_212NAGNAGNAGNAGBW1112
d_213NAGNAGNAGNAGJJ1
d_214NAGNAGNAGNAGJJ2
d_215NAGNAGNAGNAGBX1227
d_216NAGNAGNAGNAGKK1
d_217NAGNAGNAGNAGKK2
d_218MANMANMANMANKK7
d_219MANMANMANMANKK5
d_220MANMANMANMANKK6
d_221MANMANMANMANKK10
d_222MANMANMANMANKK11
d_223MANMANMANMANKK8
d_224MANMANMANMANKK9
d_225NAGNAGNAGNAGLL1
d_226NAGNAGNAGNAGLL2
d_227NAGNAGNAGNAGBY1461
d_228NAGNAGNAGNAGMM1
d_229NAGNAGNAGNAGMM2
d_230NAGNAGNAGNAGNN1
d_231NAGNAGNAGNAGNN2
d_232NAGNAGNAGNAGOO1
d_233NAGNAGNAGNAGOO2
d_234NAGNAGNAGNAGBZ1722

NCS oper: (Code: givenMatrix: (-0.464188691727, -0.84820904971, -0.255088742328), (-0.840337081892, 0.330717445793, 0.429487554936), (-0.279932933521, 0.41372379559, -0.866296816162)Vector: 35. ...NCS oper: (Code: given
Matrix: (-0.464188691727, -0.84820904971, -0.255088742328), (-0.840337081892, 0.330717445793, 0.429487554936), (-0.279932933521, 0.41372379559, -0.866296816162)
Vector: 35.1189324161, -7.36361594747, 96.3213798242)

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Receptor-like protein kinase BRI1-like 3 / BRASSINOSTEROID INSENSITIVE 1-like protein 3


Mass: 83619.508 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Strain: Col-0 / Gene: BRL3, At3g13380, MRP15.1 / Production host: Trichoplusia ni (cabbage looper)
References: UniProt: Q9LJF3, receptor protein-tyrosine kinase, non-specific serine/threonine protein kinase

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Sugars , 5 types, 22 molecules

#2: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 11
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#3: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1721.527 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-3[DManpa1-2DManpa1-6]DManpa1-6[DManpa1-2DManpa1-3]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,10,9/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-f1_d2-e1_f3-g1_f6-i1_g2-h1_i2-j1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}}}}LINUCSPDB-CARE
#4: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1883.668 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-2DManpa1-3[DManpa1-2DManpa1-3[DManpa1-2DManpa1-6]DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,11,10/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-g1_d2-e1_e2-f1_g3-h1_g6-j1_h2-i1_j2-k1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}}}}LINUCSPDB-CARE
#5: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5]/1-1-2/a4-b1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}LINUCSPDB-CARE
#7: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 2 types, 3 molecules

#6: Chemical ChemComp-A1JMI / 6-deoxocastasterone / (2~{R},3~{S},5~{R},8~{R},9~{S},10~{S},13~{S},14~{S},17~{R})-17-[(2~{S},3~{R},4~{R},5~{S})-5,6-dimethyl-3,4-bis(oxidanyl)heptan-2-yl]-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1~{H}-cyclopenta[a]phenanthrene-2,3-diol


Mass: 450.694 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Formula: C28H50O4
#8: Chemical ChemComp-ACT / ACETATE ION


Mass: 59.044 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C2H3O2

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.28 Å3/Da / Density % sol: 62.5 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4
Details: 23% PEG 3350, 0.2 M Li2So4, 0.1 M citric acid pH 4.0, 25% glycerol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 0.99999 Å
DetectorType: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Dec 4, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.99999 Å / Relative weight: 1
ReflectionResolution: 3.2→48.11 Å / Num. obs: 32179 / % possible obs: 91.7 % / Observed criterion σ(I): -3 / Redundancy: 3.9 % / Biso Wilson estimate: 114.55 Å2 / CC1/2: 0.996 / Rrim(I) all: 0.197 / Net I/σ(I): 5.5
Reflection shellResolution: 3.2→3.39 Å / Redundancy: 3.9 % / Mean I/σ(I) obs: 1 / Num. unique obs: 5210 / CC1/2: 0.23 / Rrim(I) all: 3.851 / % possible all: 92

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Processing

Software
NameVersionClassification
PHENIX1.21.1_5286refinement
XDSdata reduction
XSCALEdata scaling
RefinementMethod to determine structure: FOURIER SYNTHESIS / Resolution: 3.2→48.11 Å / SU ML: 0.6776 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 39.028
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2812 1585 5.07 %
Rwork0.2344 29654 -
obs0.2369 31239 89.16 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 130.94 Å2
Refinement stepCycle: LAST / Resolution: 3.2→48.11 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10832 0 770 0 11602
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002511843
X-RAY DIFFRACTIONf_angle_d0.64416118
X-RAY DIFFRACTIONf_chiral_restr0.04252068
X-RAY DIFFRACTIONf_plane_restr0.00421993
X-RAY DIFFRACTIONf_dihedral_angle_d12.47094990
Refine LS restraints NCSType: Torsion NCS / Rms dev position: 5.01965520347 Å
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.2-3.30.4751180.46932131X-RAY DIFFRACTION69.48
3.3-3.420.45971470.40962673X-RAY DIFFRACTION89.16
3.42-3.560.41461520.37072783X-RAY DIFFRACTION92.47
3.56-3.720.38721450.31992739X-RAY DIFFRACTION91.18
3.72-3.920.34021450.27972736X-RAY DIFFRACTION89.53
3.92-4.160.30611340.22552394X-RAY DIFFRACTION79.4
4.16-4.480.25261450.20882829X-RAY DIFFRACTION93.85
4.48-4.930.26141540.19952897X-RAY DIFFRACTION96.28
4.93-5.650.26291550.21842880X-RAY DIFFRACTION95.11
5.65-7.110.29011400.24522815X-RAY DIFFRACTION93.07
7.11-48.110.22231500.18452777X-RAY DIFFRACTION91.7
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.7105192117930.0409021024453-0.3494813352980.27957760275-0.6723989857111.63807475769-0.323252186784-0.2442106093170.2247421314550.150180959039-0.2280920670080.2075913131471.33236752045-0.6300627748290.5278080891751.78953788258-0.2556290655110.1786973629371.783801505930.02073147051031.21434613984-30.0804963807-14.123779339294.510899812
21.775984051850.0618698909325-0.2503050907282.775734761680.3311889689291.82823661086-0.188677129954-0.119045393175-0.1979241911250.717188218529-0.203354518066-0.167976980920.7039221914960.5262032216430.4515071812491.187169790970.1126445500590.1235272514991.201361404920.2047598307061.05015070261-11.0276376844-6.2045789149286.5572940552
31.33551694326-1.08548380195-1.15748622341.203995894810.7664335118910.8105655849270.298319256780.2076153856580.0537428027889-0.472636745013-0.297878936954-0.19944565419-0.165246709599-0.3714161989770.01753952489670.8804443328880.292808597408-0.04021196342141.138275957780.1380948327880.823076776789-20.988971314726.162717313171.54430642
43.41580094705-1.48626238373-2.077489148081.203850627140.1454147327793.777186678030.5204161638620.1043177347870.254199892198-0.01579142969620.0414603439904-0.12906116805-1.62602024656-0.270587646646-0.3640149552721.338114210380.0450396527585-0.04316829833171.002634100750.00691790950630.953385296456-24.148574518238.242595945992.0033115756
51.23900842405-0.131522009704-0.06943805280631.36967343940.642798738512.21042931290.0618010153127-0.0294116392481-0.1906019165970.301416950383-0.392442417902-0.115725190398-0.08400209553050.2325832132880.3386230769520.9232111815950.2075495839170.1159316519431.159610760070.2149979570610.889337833963-24.979690296239.4352884332109.659887793
62.22632794109-0.974092475154-0.5736426068583.72431577359-0.1051780159972.198006988070.1168263201271.12961410793-0.176642660068-0.688456614312-0.29997562657-0.8852436494770.3466960495440.09521763799050.249286462011.34834876035-0.1058234270520.1131457985141.26907819073-0.02203799188041.0368583610835.767038623249.59885618315.5965851088
70.55550395267-0.851010241275-0.3092882669882.779792900590.2205867801390.914003001005-0.373045233246-0.0954662161624-0.1046509906810.4695969210620.101250263773-0.004582933342390.213099304450.06084654850660.3205204286860.801862274331-0.0820155887655-0.06972301731870.796901225976-0.01570778244080.6329151712527.876192929246.36988796545.0758106671
81.2764763442-0.5335479905070.4103226273273.4723793353-0.6617969236211.59637688078-0.04550757194630.2325706307940.11848392051-0.1171320568010.2914575233340.241494596175-0.520426871565-0.57851010128-0.2607622693330.899166725244-0.01464403325270.001767474994860.941635582590.1073844993110.828637104506-8.5010884909966.451373385237.0039000893
91.380064473450.4384094655130.8197212764962.539243043530.3850203701092.71094965598-0.1473246417420.2561052016420.0195755375632-0.9631954268980.115354213566-0.0720887199598-0.585671482789-0.686313385286-0.02432255833121.33651444740.114333725540.0685075591481.114345667590.1418756913790.912871242554-17.825317707575.766017326621.7660589055
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 31 through 87 )AA31 - 871 - 51
22chain 'A' and (resid 88 through 219 )AA88 - 21952 - 183
33chain 'A' and (resid 220 through 553 )AA220 - 553184 - 517
44chain 'A' and (resid 554 through 623 )AA554 - 623518 - 587
55chain 'A' and (resid 624 through 757 )AA624 - 757588 - 721
66chain 'B' and (resid 31 through 127 )BB31 - 1271 - 97
77chain 'B' and (resid 128 through 553 )BB128 - 55398 - 523
88chain 'B' and (resid 554 through 656 )BB554 - 656524 - 626
99chain 'B' and (resid 657 through 757 )BB657 - 757627 - 727

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