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- PDB-9pfo: Structure of POU2F3 POU domains bound to coactivator OCA-T2 and D... -

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Basic information

Entry
Database: PDB / ID: 9pfo
TitleStructure of POU2F3 POU domains bound to coactivator OCA-T2 and DNA (2.1 angstrom resolution)
Components
  • DNA containing POU domain recognition element octamer (antisense strand)
  • DNA containing POU domain recognition element octamer (sense strand)
  • POU class 2 homeobox associating factor 3
  • POU domain, class 2, transcription factor 3
KeywordsTRANSCRIPTION / transcription factor / DNA binding / coactivator complex
Function / homology
Function and homology information


POU domain binding / host-mediated suppression of viral transcription / epidermis development / keratinocyte differentiation / wound healing / sequence-specific double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific / transcription regulator complex / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific ...POU domain binding / host-mediated suppression of viral transcription / epidermis development / keratinocyte differentiation / wound healing / sequence-specific double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific / transcription regulator complex / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / transcription coactivator activity / nuclear body / RNA polymerase II cis-regulatory region sequence-specific DNA binding / regulation of transcription by RNA polymerase II / chromatin / nucleolus / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / identical protein binding / nucleus / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Colorectal cancer-associated protein 2 / : / OCA domain profile. / Octamer-binding transcription factor / POU-specific domain / POU domain / Pou domain - N-terminal to homeobox domain / POU-specific (POUs) domain signature 1. / POU-specific (POUs) domain signature 2. / POU-specific (POUs) domain profile. ...Colorectal cancer-associated protein 2 / : / OCA domain profile. / Octamer-binding transcription factor / POU-specific domain / POU domain / Pou domain - N-terminal to homeobox domain / POU-specific (POUs) domain signature 1. / POU-specific (POUs) domain signature 2. / POU-specific (POUs) domain profile. / Found in Pit-Oct-Unc transcription factors / : / Homeobox, conserved site / 'Homeobox' domain signature. / Homeodomain / 'Homeobox' domain profile. / Homeodomain / Homeobox domain / Lambda repressor-like, DNA-binding domain superfamily / Homeobox-like domain superfamily
Similarity search - Domain/homology
DNA / DNA (> 10) / POU class 2 homeobox associating factor 3 / POU domain, class 2, transcription factor 3
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å
AuthorsIpsaro, J.J. / Alpsoy, A. / Vakoc, C.R. / Joshua-Tor, L.
Funding support United States, 8items
OrganizationGrant numberCountry
National Institutes of Health/National Cancer Institute (NIH/NCI)CA045508 United States
National Institutes of Health/National Cancer Institute (NIH/NCI)CA013106 United States
National Institutes of Health/National Cancer Institute (NIH/NCI)CA242919 United States
Department of Defense (DOD, United States)W81XWH1910317 United States
Howard Hughes Medical Institute (HHMI) United States
Other privateCold Spring Harbor Laboratory and Northwell Health Affiliation
Other privatePershing Square Sohn Cancer Research Alliance
Other privateTreeline Biosciences
CitationJournal: To Be Published
Title: Structural Basis of DNA-Dependent Coactivator Recruitment by the Tuft Cell Master Regulator POU2F3
Authors: Alpsoy, A. / Ipsaro, J.J. / Skopelitis, D. / Pal, S. / Chung, F.S. / Carpenter, S. / Desmarais, J. / Wu, X.S. / Chang, K. / DiMare, M.T. / Harten, E. / Bergman, S. / Kinney, J. / Engelman, J. ...Authors: Alpsoy, A. / Ipsaro, J.J. / Skopelitis, D. / Pal, S. / Chung, F.S. / Carpenter, S. / Desmarais, J. / Wu, X.S. / Chang, K. / DiMare, M.T. / Harten, E. / Bergman, S. / Kinney, J. / Engelman, J.A. / Bhang, H.C. / Joshua-Tor, L. / Vakoc, C.R.
History
DepositionJul 6, 2025Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 1, 2025Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: POU domain, class 2, transcription factor 3
B: POU class 2 homeobox associating factor 3
C: DNA containing POU domain recognition element octamer (sense strand)
D: DNA containing POU domain recognition element octamer (antisense strand)
E: POU domain, class 2, transcription factor 3
F: POU class 2 homeobox associating factor 3
G: DNA containing POU domain recognition element octamer (sense strand)
H: DNA containing POU domain recognition element octamer (antisense strand)
I: POU domain, class 2, transcription factor 3
J: POU class 2 homeobox associating factor 3
K: DNA containing POU domain recognition element octamer (sense strand)
L: DNA containing POU domain recognition element octamer (antisense strand)
M: POU domain, class 2, transcription factor 3
N: POU class 2 homeobox associating factor 3
O: DNA containing POU domain recognition element octamer (sense strand)
P: DNA containing POU domain recognition element octamer (antisense strand)


Theoretical massNumber of molelcules
Total (without water)127,59216
Polymers127,59216
Non-polymers00
Water13,998777
1
A: POU domain, class 2, transcription factor 3
B: POU class 2 homeobox associating factor 3
C: DNA containing POU domain recognition element octamer (sense strand)
D: DNA containing POU domain recognition element octamer (antisense strand)


Theoretical massNumber of molelcules
Total (without water)31,8984
Polymers31,8984
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6830 Å2
ΔGint-46 kcal/mol
Surface area13840 Å2
MethodPISA
2
E: POU domain, class 2, transcription factor 3
F: POU class 2 homeobox associating factor 3
G: DNA containing POU domain recognition element octamer (sense strand)
H: DNA containing POU domain recognition element octamer (antisense strand)


Theoretical massNumber of molelcules
Total (without water)31,8984
Polymers31,8984
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6750 Å2
ΔGint-40 kcal/mol
Surface area13160 Å2
MethodPISA
3
I: POU domain, class 2, transcription factor 3
J: POU class 2 homeobox associating factor 3
K: DNA containing POU domain recognition element octamer (sense strand)
L: DNA containing POU domain recognition element octamer (antisense strand)


Theoretical massNumber of molelcules
Total (without water)31,8984
Polymers31,8984
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6670 Å2
ΔGint-41 kcal/mol
Surface area13730 Å2
MethodPISA
4
M: POU domain, class 2, transcription factor 3
N: POU class 2 homeobox associating factor 3
O: DNA containing POU domain recognition element octamer (sense strand)
P: DNA containing POU domain recognition element octamer (antisense strand)


Theoretical massNumber of molelcules
Total (without water)31,8984
Polymers31,8984
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6790 Å2
ΔGint-41 kcal/mol
Surface area13930 Å2
MethodPISA
Unit cell
Length a, b, c (Å)79.019, 95.802, 85.637
Angle α, β, γ (deg.)90.00, 101.24, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein
POU domain, class 2, transcription factor 3 / Octamer-binding protein 11 / Oct-11 / Octamer-binding transcription factor 11 / OTF-11 / ...Octamer-binding protein 11 / Oct-11 / Octamer-binding transcription factor 11 / OTF-11 / Transcription factor PLA-1 / Transcription factor Skn-1


Mass: 18503.135 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: POU2F3, OTF11, PLA1 / Plasmid: pHIS-Parallel-GFP / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): RIPL / References: UniProt: Q9UKI9
#2: Protein/peptide
POU class 2 homeobox associating factor 3 / Cancer susceptibility candidate protein 13 / Colorectal cancer-associated protein 2 / Protein OCA-T2


Mass: 4218.865 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: POU2AF3, C11orf93, CASC13, COLCA2 / Plasmid: pGEX-4T1 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): RIPL / References: UniProt: A8K830
#3: DNA chain
DNA containing POU domain recognition element octamer (sense strand)


Mass: 4657.060 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#4: DNA chain
DNA containing POU domain recognition element octamer (antisense strand)


Mass: 4518.959 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 777 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.49 Å3/Da / Density % sol: 50.64 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 6
Details: 0.1 M MMT (1:2:2 molar ratio of DL-malic acid:MES:Tris base), pH 6.0, 25% PEG-1500

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: NSLS-II / Beamline: 17-ID-1 / Wavelength: 0.92011 Å
DetectorType: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Feb 2, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.92011 Å / Relative weight: 1
ReflectionResolution: 2.1→29.85 Å / Num. obs: 71525 / % possible obs: 97.9 % / Redundancy: 3.1 % / CC1/2: 0.872 / Rmerge(I) obs: 0.246 / Rpim(I) all: 0.173 / Rrim(I) all: 0.303 / Χ2: 0.98 / Net I/σ(I): 4.6 / Num. measured all: 224565
Reflection shellResolution: 2.1→2.15 Å / % possible obs: 97.8 % / Redundancy: 3.2 % / Rmerge(I) obs: 0.676 / Num. measured all: 14046 / Num. unique obs: 4421 / CC1/2: 0.531 / Rpim(I) all: 0.473 / Rrim(I) all: 0.83 / Χ2: 1.38 / Net I/σ(I) obs: 2.9

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Processing

Software
NameVersionClassification
XDSFebruary 5, 2021data reduction
pointless1.12.10data scaling
Aimless0.7.7data scaling
PHASER2.8.3phasing
Coot0.9.6model building
PHENIX1.20.1_4487refinement
MolProbitymodel building
PDB_EXTRACTdata extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.1→29.83 Å / SU ML: 0.25 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 28.77 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2589 3510 4.97 %
Rwork0.2192 --
obs0.2211 70600 96.64 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.1→29.83 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5458 2436 0 777 8671
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0088263
X-RAY DIFFRACTIONf_angle_d0.96811565
X-RAY DIFFRACTIONf_dihedral_angle_d21.7313399
X-RAY DIFFRACTIONf_chiral_restr0.0471271
X-RAY DIFFRACTIONf_plane_restr0.0111062
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.1-2.130.33781250.26712652X-RAY DIFFRACTION95
2.13-2.160.30781430.25362608X-RAY DIFFRACTION95
2.16-2.190.29841320.25292644X-RAY DIFFRACTION95
2.19-2.230.34161430.25342645X-RAY DIFFRACTION96
2.23-2.260.30971190.2452660X-RAY DIFFRACTION96
2.26-2.30.30671180.2332690X-RAY DIFFRACTION96
2.3-2.340.26511290.23172701X-RAY DIFFRACTION97
2.34-2.390.26411390.23862685X-RAY DIFFRACTION97
2.39-2.440.32191500.23572636X-RAY DIFFRACTION97
2.44-2.490.26951460.23142701X-RAY DIFFRACTION97
2.49-2.550.29611510.23092662X-RAY DIFFRACTION96
2.55-2.610.31751670.22782661X-RAY DIFFRACTION97
2.61-2.680.25441480.22132695X-RAY DIFFRACTION98
2.68-2.760.24031460.21072719X-RAY DIFFRACTION98
2.76-2.850.25831400.20852687X-RAY DIFFRACTION97
2.85-2.950.27331620.21892706X-RAY DIFFRACTION98
2.95-3.070.2791390.23072747X-RAY DIFFRACTION99
3.07-3.210.27631530.21882690X-RAY DIFFRACTION98
3.21-3.380.21961490.19932718X-RAY DIFFRACTION97
3.38-3.590.22461240.20282735X-RAY DIFFRACTION98
3.59-3.870.22741460.20712696X-RAY DIFFRACTION97
3.87-4.250.2451690.19912629X-RAY DIFFRACTION96
4.25-4.870.23141330.20782618X-RAY DIFFRACTION93
4.87-6.120.23911340.22282694X-RAY DIFFRACTION95
6.12-29.830.23091050.21662811X-RAY DIFFRACTION97
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.57674.32630.09927.4483-0.45323.29891.0065-0.48450.02671.2208-0.68170.5361-0.29450.0712-0.16450.5444-0.0359-0.08060.2683-0.04830.220233.836517.423320.441
24.0575-1.29320.94583.70430.57733.77490.405-0.2539-0.0190.3666-0.2642-0.73750.25650.3561-0.22370.3752-0.1299-0.14740.27760.02760.402750.341616.20413.0856
31.90970.89650.10662.66380.2223.91760.0982-0.2438-0.01350.1996-0.0745-0.1269-0.03670.2124-0.00990.2648-0.0028-0.05570.21330.01220.13840.63412.091114.8313
41.86231.34421.6113.19743.23134.15930.3285-0.1312-0.22360.56050.2053-0.812-0.52310.4332-0.57660.647-0.1466-0.13810.27810.00540.562650.31177.10446.9564
53.89870.2802-0.00434.2572-0.46253.0249-0.04430.2087-0.2549-0.55530.049-0.05880.24770.3886-0.00740.2864-0.01310.01660.2286-0.01690.123138.9699-5.2036-13.4918
63.53150.8697-0.46912.92872.91443.2828-0.19040.32710.4351-0.5362-0.1062-0.0552-0.4222-0.05180.28450.28520.0059-0.05070.16160.04520.252227.07479.1-2.715
78.53652.21370.44974.43371.77794.10280.184-1.20051.1159-0.3151-0.61320.6052-0.7779-0.8420.44390.44350.169-0.10810.584-0.13460.297319.79615.634815.834
83.20660.1378-1.2120.83471.55366.3848-0.14210.2098-0.23360.0718-0.08880.0157-0.2943-0.11110.22260.181-0.0139-0.05850.2390.01430.587855.2441-7.131938.6578
92.0971-0.0863-0.0321.63740.18851.90530.04490.209-0.0488-0.0473-0.0564-0.0424-0.0631-0.07810.00420.15640.0357-0.01220.21150.00840.598159.847-0.922339.7039
103.3446-2.1004-0.02324.3809-0.18584.43410.11370.18070.2559-0.1893-0.2196-0.179-0.09880.36630.09490.1159-0.0171-0.01190.21430.04190.370487.547715.729640.6952
117.58980.427-2.11978.2347-0.56655.10160.1847-0.46480.3780.5727-0.15770.073-0.11430.32260.0630.08770.0171-0.01390.21440.00340.313579.513213.182648.1412
125.94842.60010.53333.11772.88473.66030.3931-1.20240.25040.6221-0.1346-0.42650.20250.6634-0.15580.2633-0.0836-0.06990.51630.11840.505472.31050.756154.6095
133.26180.4634-3.21419.0055-3.02257.28860.6556-0.7539-0.25520.9806-0.3384-0.47890.5948-0.9273-0.25830.5313-0.2299-0.17090.6660.06630.544554.2157-6.615463.0743
144.411-1.1332-0.3372.60611.44821.3113-0.0932-0.1519-0.47790.20440.01580.28760.1336-0.02140.03590.21460.0131-0.05220.14290.0440.28665.029728.2249-0.5321
153.62891.44210.83653.1293-0.00953.55680.03540.0125-0.03240.07920.00130.05970.06270.1869-0.00620.17880.0995-0.0350.18660.01050.254791.6469.26060.5208
166.0691-1.90612.75350.86830.14795.0321-0.25630.7340.9358-0.6822-0.1684-1.54220.17560.66440.28160.25510.0656-0.03630.27640.08650.731882.352823.3033-12.9899
178.78341.08850.17244.0396-2.18398.4158-0.58080.55780.1904-0.51680.0579-1.1225-0.9965-0.17980.48470.42620.0895-0.06070.28330.0650.416668.511131.3598-23.115
182.92514.7688-1.26087.9681-1.39555.86860.1803-0.50260.36421.3641-0.15980.1666-0.6024-0.35090.0120.48240.0857-0.08920.351-0.08340.350230.408521.825460.6498
191.61770.35670.21952.14580.67072.9516-0.081-0.2277-0.04140.48760.1789-0.3851-0.0670.1538-0.10210.24670.0422-0.08820.1921-0.03530.196536.705614.776655.1121
200.41540.50930.38165.0681-0.06570.3924-0.4988-0.274-0.3932-0.99670.5019-0.9689-0.01810.43360.02330.9380.50350.19970.9394-0.0710.850251.444115.318658.2388
218.40140.44095.88393.63760.91137.42830.0463-0.2796-0.56320.01650.7468-0.39030.01370.5147-0.67060.2507-0.0118-0.03240.2336-0.03520.414940.41822.813743.1469
224.46635.8474-1.12398.6285-2.00715.9581-0.48751.21020.2648-1.43020.30790.44320.057-0.05130.04850.26450.0799-0.11990.343-0.04670.287129.3482-2.796124.5307
237.23671.06381.54095.0743-1.73853.29320.09170.4084-0.5477-0.46320.0942-0.4510.4830.1526-0.17740.2968-0.00320.05350.2608-0.13190.464935.1131-11.348329.6771
247.3935-0.7775-0.43132.06270.054.1931-0.0004-0.09370.0132-0.3050.0940.46510.062-0.0911-0.0920.1520.0041-0.07950.1158-0.03930.344825.4293-4.152235.3499
253.96111.4402-3.65927.11062.85485.89270.3410.0902-0.1975-0.4524-0.11570.4571-0.4975-0.8501-0.26770.14190.0539-0.08850.27280.01760.405417.66219.049639.4689
268.0044-0.9078-1.28192.00782.30515.48030.3435-1.16080.32830.4947-0.26760.4338-0.7455-1.6285-0.06430.49380.11510.01870.5827-0.04170.34612.841419.03759.3296
270.50070.07360.70816.39071.60081.729-0.0473-0.09220.00320.5082-0.0619-0.0787-0.2804-0.01850.09430.1854-0.0001-0.00460.2971-0.00560.143837.73556.19351.2055
281.66721.80030.74045.00210.25752.95870.05060.0945-0.0741-0.0497-0.0608-0.1867-0.07690.179-0.00590.11330.0242-0.01780.2316-0.0190.13537.44073.1328-1.1478
292.59612.08130.48391.4806-0.0321.36090.0451-0.15530.00380.1652-0.08340.0723-0.0396-0.16890.0240.13970.0259-0.00170.36630.01790.475569.72523.327543.9834
302.6411.78820.66693.57341.27710.88140.0910.1372-0.0222-0.1383-0.1362-0.1075-0.0952-0.11120.06190.16060.0035-0.02660.36670.01820.425172.05866.363244.4523
311.3628-1.53060.12953.5434-0.72210.07330.08050.1427-0.0556-0.0419-0.10510.04510.054-0.02550.02940.1952-0.0056-0.07970.24370.00780.18978.065720.7354-2.7483
322.7155-1.62680.16154.01430.62221.0930.06990.1299-0.02150.0446-0.0673-0.16850.02050.0333-0.01460.18530.0507-0.0680.2570.04790.24580.41517.6946-2.7887
330.38490.336-0.36416.34730.88250.743-0.05020.0433-0.19940.1845-0.0698-0.2374-0.0537-0.01090.08310.08820.001-0.03550.2062-0.03420.166929.49986.533343.7419
341.44081.19520.22574.64790.54091.9602-0.00160.0584-0.1727-0.0712-0.16260.10910.01370.13330.13820.05670.03370.0120.204-0.05960.198928.9243.435941.7722
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 182 through 204 )
2X-RAY DIFFRACTION2chain 'A' and (resid 205 through 220 )
3X-RAY DIFFRACTION3chain 'A' and (resid 221 through 257 )
4X-RAY DIFFRACTION4chain 'A' and (resid 258 through 289 )
5X-RAY DIFFRACTION5chain 'A' and (resid 290 through 341 )
6X-RAY DIFFRACTION6chain 'B' and (resid 5 through 16 )
7X-RAY DIFFRACTION7chain 'B' and (resid 17 through 30 )
8X-RAY DIFFRACTION8chain 'E' and (resid 186 through 225 )
9X-RAY DIFFRACTION9chain 'E' and (resid 226 through 289 )
10X-RAY DIFFRACTION10chain 'E' and (resid 290 through 321 )
11X-RAY DIFFRACTION11chain 'E' and (resid 322 through 341 )
12X-RAY DIFFRACTION12chain 'F' and (resid 6 through 16 )
13X-RAY DIFFRACTION13chain 'F' and (resid 17 through 33 )
14X-RAY DIFFRACTION14chain 'I' and (resid 184 through 289 )
15X-RAY DIFFRACTION15chain 'I' and (resid 290 through 341 )
16X-RAY DIFFRACTION16chain 'J' and (resid 6 through 16 )
17X-RAY DIFFRACTION17chain 'J' and (resid 17 through 30 )
18X-RAY DIFFRACTION18chain 'M' and (resid 186 through 204 )
19X-RAY DIFFRACTION19chain 'M' and (resid 205 through 258 )
20X-RAY DIFFRACTION20chain 'M' and (resid 259 through 277 )
21X-RAY DIFFRACTION21chain 'M' and (resid 278 through 289 )
22X-RAY DIFFRACTION22chain 'M' and (resid 290 through 302 )
23X-RAY DIFFRACTION23chain 'M' and (resid 303 through 321 )
24X-RAY DIFFRACTION24chain 'M' and (resid 322 through 341 )
25X-RAY DIFFRACTION25chain 'N' and (resid 4 through 16 )
26X-RAY DIFFRACTION26chain 'N' and (resid 17 through 31 )
27X-RAY DIFFRACTION27chain 'C' and (resid 1 through 15 )
28X-RAY DIFFRACTION28chain 'D' and (resid 1 through 15 )
29X-RAY DIFFRACTION29chain 'G' and (resid 1 through 15 )
30X-RAY DIFFRACTION30chain 'H' and (resid 1 through 15 )
31X-RAY DIFFRACTION31chain 'K' and (resid 1 through 15 )
32X-RAY DIFFRACTION32chain 'L' and (resid 1 through 15 )
33X-RAY DIFFRACTION33chain 'O' and (resid 1 through 15 )
34X-RAY DIFFRACTION34chain 'P' and (resid 1 through 15 )

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