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- PDB-9pdj: Composite map of open form of PIC-Med-SWI/SNF -

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Basic information

Entry
Database: PDB / ID: 9pdj
TitleComposite map of open form of PIC-Med-SWI/SNF
Components
  • (DNA-directed RNA polymerase II subunit ...) x 7
  • (DNA-directed RNA polymerases I, II, and III subunit ...) x 5
  • (General transcription and DNA repair factor IIH helicase subunit ...) x 2
  • (General transcription and DNA repair factor IIH subunit ...) x 5
  • (Mediator of RNA polymerase II transcription subunit ...) x 21
  • (SWI/SNF chromatin-remodeling complex subunit ...) x 2
  • (Transcription initiation factor ...) x 8
  • (Transcription regulatory protein ...) x 3
  • (promoter DNA) x 2
  • Actin-like protein ARP9
  • Actin-related protein 7
  • RNA polymerase II transcription factor B subunit 3
  • Regulator of Ty1 transposition protein 102
  • SWI/SNF complex subunit SWI3
  • SWI/SNF global transcription activator complex subunit SWP82
  • TATA-box-binding protein
  • Transcription elongation factor S-II
  • unsigned peptide of SWISNF
KeywordsTRANSCRIPTION/DNA / SWI/SNF / PIC / TRANSCRIPTION / TRANSCRIPTION-DNA complex
Function / homology
Function and homology information


TFIIE-class transcription factor complex binding / regulation of establishment of protein localization to chromosome / carbon catabolite activation of transcription / meiotic gene conversion / NuA3b histone acetyltransferase complex / positive regulation of cell adhesion involved in single-species biofilm formation / NuA3a histone acetyltransferase complex / NuA3 histone acetyltransferase complex / positive regulation of mating type switching / positive regulation of invasive growth in response to glucose limitation ...TFIIE-class transcription factor complex binding / regulation of establishment of protein localization to chromosome / carbon catabolite activation of transcription / meiotic gene conversion / NuA3b histone acetyltransferase complex / positive regulation of cell adhesion involved in single-species biofilm formation / NuA3a histone acetyltransferase complex / NuA3 histone acetyltransferase complex / positive regulation of mating type switching / positive regulation of invasive growth in response to glucose limitation / HDACs deacetylate histones / regulation of mitotic recombination / TFIIA-class transcription factor complex binding / aggrephagy / transcription open complex formation at RNA polymerase II promoter / RNA polymerase III transcription regulatory region sequence-specific DNA binding / TFIIH-class transcription factor complex binding / RNA polymerase III preinitiation complex assembly / phosphatidylinositol-5-phosphate binding / RNA polymerase II promoter clearance / regulation of mRNA 3'-end processing / RNA polymerase I general transcription initiation factor binding / positive regulation of mitotic recombination / core mediator complex / transcription factor TFIIE complex / nucleotide-excision repair factor 3 complex / nucleotide-excision repair, preincision complex assembly / DNA translocase activity / negative regulation of ribosomal protein gene transcription by RNA polymerase II / TFIIF-class transcription factor complex binding / transcription factor TFIIK complex / DNA strand invasion / rDNA binding / transcriptional start site selection at RNA polymerase II promoter / transcription factor TFIIF complex / RPB4-RPB7 complex / phosphatidylinositol-3-phosphate binding / SUMOylation of chromatin organization proteins / mediator complex / transcription factor TFIIIB complex / transcription factor TFIIA complex / histone H3K14ac reader activity / RNA polymerase I preinitiation complex assembly / transcription factor TFIIH core complex / transcription factor TFIIH holo complex / Ino80 complex / cyclin-dependent protein serine/threonine kinase activator activity / RSC-type complex / transcription elongation factor activity / DNA 5'-3' helicase / nucleosome disassembly / DNA binding, bending / ATP-dependent chromatin remodeler activity / transcription preinitiation complex / poly(A)+ mRNA export from nucleus / SWI/SNF complex / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / RNA Polymerase I Transcription Initiation / transcription factor TFIID complex / double-strand break repair via break-induced replication / 5'-3' DNA helicase activity / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA polymerase II general transcription initiation factor activity / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / Formation of TC-NER Pre-Incision Complex / mRNA Capping / Estrogen-dependent gene expression / RNA polymerase II transcribes snRNA genes / RNA Polymerase I Promoter Escape / TP53 Regulates Transcription of DNA Repair Genes / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Polymerase II Pre-transcription Events / ATPase activator activity / RNA-templated transcription / positive regulation of transcription by RNA polymerase I / TFIIB-class transcription factor binding / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / nuclear chromosome / Gap-filling DNA repair synthesis and ligation in TC-NER / RNA polymerase II complex binding / termination of RNA polymerase II transcription / TFIID-class transcription factor complex binding / histone H4 reader activity / DNA 3'-5' helicase / protein phosphatase activator activity / termination of RNA polymerase I transcription / Dual incision in TC-NER / positive regulation of transcription initiation by RNA polymerase II / positive regulation of RNA polymerase II transcription preinitiation complex assembly / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / positive regulation of translational initiation / transcription initiation at RNA polymerase I promoter / transcription by RNA polymerase III / nuclear-transcribed mRNA catabolic process
Similarity search - Function
Mediator of RNA polymerase II transcription subunit 3 / Mediator complex, subunit Med2, fungi / Mediator complex subunit 2 / Mediator complex subunit 3 fungal / : / Mediator complex, subunit Med15, fungi / Mediator complex, subunit Med5, fungi / Gal11, coactivator domain / Mediator complex subunit 15 / Mediator complex subunit Med5 ...Mediator of RNA polymerase II transcription subunit 3 / Mediator complex, subunit Med2, fungi / Mediator complex subunit 2 / Mediator complex subunit 3 fungal / : / Mediator complex, subunit Med15, fungi / Mediator complex, subunit Med5, fungi / Gal11, coactivator domain / Mediator complex subunit 15 / Mediator complex subunit Med5 / Gal11 activator-binding domain (ABD1) / Mediator complex subunit 15, KIX domain / KIX domain / Mediator complex, subunit Med19, fungi / Rox3 mediator complex subunit / SAS complex subunit SAS5/transcription initiation factor TFIID subunit 14 / Mediator of RNA polymerase II transcription subunit 22, Saccharomycetes / Mediator of RNA polymerase II transcription subunit 20 / Transcription regulator protein Rtt102 / Rtt102p-like transcription regulator protein / : / Chromatin-remodelling complex, RSC SWI/SNF subunit Rsc7/Swp82 / Chromatin remodelling complex Rsc7/Swp82 subunit / Mediator complex, subunit Med6, fungi / : / Mediator complex subunit 16, C-terminal / Mediator complex, subunit Med16 / : / Mediator complex subunit 16, N-terminal / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / : / Tfa2 E-tether / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / : / SOH1 / Subunit 21 of Mediator complex / SWIFT domain / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med7 superfmaily / : / MED7 protein / YEATS / Mediator complex, subunit Med20 / SMARCC, C-terminal / : / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / SWIRM-associated region 1 / Mediator complex, subunit Med6 / Mediator complex, subunit Med14 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Mediator complex subunit MED14 / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / SNF5/SMARCB1/INI1 / Bacterial type XPD DNA helicase, FeS cluster domain / SNF5 / SMARCB1 / INI1 / Transcription elongation factor, TFIIS / Transcription elongation factor, IIS-type / Transcription factor S-II (TFIIS), central domain / Domain in the central regions of transcription elongation factor S-II (and elsewhere) / Transcription elongation factor S-II, central domain superfamily / Transcription elongation factor S-II, central domain / TFIIS central domain profile. / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / : / YEATS superfamily / YEATS family / YEATS domain profile. / QLQ / Mediator complex, subunit Med1 / Mediator of RNA polymerase II transcription subunit 1 / Glutamine-Leucine-Glutamine, QLQ / QLQ domain profile. / QLQ / TFIIS N-terminal domain profile. / Snf2-ATP coupling, chromatin remodelling complex / Snf2, ATP coupling domain / Snf2-ATP coupling, chromatin remodelling complex / TFIIS helical bundle-like domain / Transcription factor IIS, N-terminal / Transcription factor TFIIE beta subunit, DNA-binding domain / Transcription initiation factor TFIIE, beta subunit
Similarity search - Domain/homology
IRON/SULFUR CLUSTER / : / : / DNA / DNA (> 10) / DNA-directed RNA polymerase II subunit RPB1 / General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / Transcription elongation factor S-II / DNA-directed RNA polymerase II subunit RPB2 / SWI/SNF chromatin-remodeling complex subunit SWI1 ...IRON/SULFUR CLUSTER / : / : / DNA / DNA (> 10) / DNA-directed RNA polymerase II subunit RPB1 / General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / Transcription elongation factor S-II / DNA-directed RNA polymerase II subunit RPB2 / SWI/SNF chromatin-remodeling complex subunit SWI1 / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB3 / SWI/SNF chromatin-remodeling complex subunit SNF5 / Transcription regulatory protein SNF6 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 15 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / SWI/SNF chromatin-remodeling complex subunit SNF2 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Mediator of RNA polymerase II transcription subunit 19 / DNA-directed RNA polymerase II subunit RPB9 / Transcription initiation factor IIB / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 18 / SWI/SNF complex subunit SWI3 / Transcription initiation factor IIA large subunit / Transcription initiation factor IIA subunit 2 / General transcription and DNA repair factor IIH subunit TFB1 / Mediator of RNA polymerase II transcription subunit 9 / DNA-directed RNA polymerase II subunit RPB7 / Mediator of RNA polymerase II transcription subunit 20 / Transcription initiation factor TFIID subunit 14 / Transcription initiation factor IIE subunit alpha / Transcription initiation factor IIE subunit beta / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 31 / Mediator of RNA polymerase II transcription subunit 6 / DNA-directed RNA polymerase II subunit RPB11 / Mediator of RNA polymerase II transcription subunit 3 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / Transcription initiation factor IIF subunit alpha / Transcription initiation factor IIF subunit beta / SWI/SNF global transcription activator complex subunit SWP82 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 5 / Regulator of Ty1 transposition protein 102 / Transcription regulatory protein SNF12 / General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2 / General transcription and DNA repair factor IIH subunit TFB2 / RNA polymerase II transcription factor B subunit 3 / General transcription and DNA repair factor IIH subunit SSL1 / Actin-like protein ARP9 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 7 / General transcription and DNA repair factor IIH subunit TFB4 / Mediator of RNA polymerase II transcription subunit 2 / Mediator of RNA polymerase II transcription subunit 1 / Mediator of RNA polymerase II transcription subunit 4 / Actin-related protein 7 / General transcription and DNA repair factor IIH subunit TFB5 / Mediator of RNA polymerase II transcription subunit 11
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsYang, C. / Nagai, S. / Chen, D.-H.
Funding support United States, 1items
OrganizationGrant numberCountry
Other private United States
CitationJournal: To Be Published
Title: Composite map of open form of PIC-Med-SWI/SNF
Authors: Yang, C. / Nagai, S. / Chen, D.-H.
History
DepositionJun 30, 2025Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 14, 2026Provider: repository / Type: Initial release
Revision 1.0Oct 14, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A0: General transcription and DNA repair factor IIH helicase subunit XPD/RAD3
A1: General transcription and DNA repair factor IIH subunit TFB1
A2: General transcription and DNA repair factor IIH subunit TFB2
A3: RNA polymerase II transcription factor B subunit 3
A4: General transcription and DNA repair factor IIH subunit TFB4
A5: General transcription and DNA repair factor IIH subunit TFB5
A6: General transcription and DNA repair factor IIH subunit SSL1
A7: General transcription and DNA repair factor IIH helicase subunit XPB
A8: SWI/SNF chromatin-remodeling complex subunit SNF5
A9: Transcription initiation factor TFIID subunit 14
AA: DNA-directed RNA polymerase II subunit RPB1
AB: DNA-directed RNA polymerase II subunit RPB2
AC: DNA-directed RNA polymerase II subunit RPB3
AD: DNA-directed RNA polymerase II subunit RPB4
AE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
AF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
AG: DNA-directed RNA polymerase II subunit RPB7
AH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
AI: DNA-directed RNA polymerase II subunit RPB9
AJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
AK: DNA-directed RNA polymerase II subunit RPB11
AL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
AM: Transcription initiation factor IIB
AN: promoter DNA
AO: TATA-box-binding protein
AQ: Transcription initiation factor IIF subunit alpha
AR: Transcription initiation factor IIF subunit beta
AS: Transcription elongation factor S-II
AT: promoter DNA
AU: Transcription initiation factor IIA large subunit
AV: Transcription initiation factor IIA subunit 2
AW: Transcription initiation factor IIE subunit alpha
AX: Transcription initiation factor IIE subunit beta
MA: DNA-directed RNA polymerase II subunit RPB1
Ma: Mediator of RNA polymerase II transcription subunit 6
Mb: Mediator of RNA polymerase II transcription subunit 8
Mc: Mediator of RNA polymerase II transcription subunit 11
Md: Mediator of RNA polymerase II transcription subunit 17
Me: Mediator of RNA polymerase II transcription subunit 18
Mf: Mediator of RNA polymerase II transcription subunit 20
Mg: Mediator of RNA polymerase II transcription subunit 22
Mh: Mediator of RNA polymerase II transcription subunit 4
Mi: Mediator of RNA polymerase II transcription subunit 7
Mj: Mediator of RNA polymerase II transcription subunit 9
Mk: Mediator of RNA polymerase II transcription subunit 10
Ml: Mediator of RNA polymerase II transcription subunit 14
Mm: Mediator of RNA polymerase II transcription subunit 19
Mn: Mediator of RNA polymerase II transcription subunit 21
Mo: Mediator of RNA polymerase II transcription subunit 31
Mp: Mediator of RNA polymerase II transcription subunit 1
SA: Transcription regulatory protein SNF12
SB: SWI/SNF complex subunit SWI3
SC: Transcription regulatory protein SNF6
SD: SWI/SNF complex subunit SWI3
SE: SWI/SNF global transcription activator complex subunit SWP82
SF: SWI/SNF chromatin-remodeling complex subunit SNF5
SG: unsigned peptide of SWISNF
SH: Transcription regulatory protein SNF2
SI: SWI/SNF chromatin-remodeling complex subunit SWI1
SJ: Regulator of Ty1 transposition protein 102
SK: Actin-related protein 7
SL: Actin-like protein ARP9
B0: General transcription and DNA repair factor IIH helicase subunit XPD/RAD3
B1: General transcription and DNA repair factor IIH subunit TFB1
B2: General transcription and DNA repair factor IIH subunit TFB2
B3: RNA polymerase II transcription factor B subunit 3
B4: General transcription and DNA repair factor IIH subunit TFB4
B5: General transcription and DNA repair factor IIH subunit TFB5
B6: General transcription and DNA repair factor IIH subunit SSL1
B7: General transcription and DNA repair factor IIH helicase subunit XPB
B8: SWI/SNF chromatin-remodeling complex subunit SNF5
B9: Transcription initiation factor TFIID subunit 14
BA: DNA-directed RNA polymerase II subunit RPB1
BB: DNA-directed RNA polymerase II subunit RPB2
BC: DNA-directed RNA polymerase II subunit RPB3
BD: DNA-directed RNA polymerase II subunit RPB4
BE: DNA-directed RNA polymerases I, II, and III subunit RPABC1
BF: DNA-directed RNA polymerases I, II, and III subunit RPABC2
BG: DNA-directed RNA polymerase II subunit RPB7
BH: DNA-directed RNA polymerases I, II, and III subunit RPABC3
BI: DNA-directed RNA polymerase II subunit RPB9
BJ: DNA-directed RNA polymerases I, II, and III subunit RPABC5
BK: DNA-directed RNA polymerase II subunit RPB11
BL: DNA-directed RNA polymerases I, II, and III subunit RPABC4
BM: Transcription initiation factor IIB
BN: promoter DNA
BO: TATA-box-binding protein
BQ: Transcription initiation factor IIF subunit alpha
BR: Transcription initiation factor IIF subunit beta
BS: Transcription elongation factor S-II
BT: promoter DNA
BU: Transcription initiation factor IIA large subunit
BV: Transcription initiation factor IIA subunit 2
BW: Transcription initiation factor IIE subunit alpha
BX: Transcription initiation factor IIE subunit beta
mA: DNA-directed RNA polymerase II subunit RPB1
ma: Mediator of RNA polymerase II transcription subunit 6
mb: Mediator of RNA polymerase II transcription subunit 8
mc: Mediator of RNA polymerase II transcription subunit 11
md: Mediator of RNA polymerase II transcription subunit 17
me: Mediator of RNA polymerase II transcription subunit 18
mf: Mediator of RNA polymerase II transcription subunit 20
mg: Mediator of RNA polymerase II transcription subunit 22
mh: Mediator of RNA polymerase II transcription subunit 4
mi: Mediator of RNA polymerase II transcription subunit 7
mj: Mediator of RNA polymerase II transcription subunit 9
mk: Mediator of RNA polymerase II transcription subunit 10
ml: Mediator of RNA polymerase II transcription subunit 14
mm: Mediator of RNA polymerase II transcription subunit 19
mn: Mediator of RNA polymerase II transcription subunit 21
mo: Mediator of RNA polymerase II transcription subunit 31
mp: Mediator of RNA polymerase II transcription subunit 1
sA: Transcription regulatory protein SNF12
sB: SWI/SNF complex subunit SWI3
sC: Transcription regulatory protein SNF6
sD: SWI/SNF complex subunit SWI3
sE: SWI/SNF global transcription activator complex subunit SWP82
sF: SWI/SNF chromatin-remodeling complex subunit SNF5
sG: unsigned peptide of SWISNF
sH: Transcription regulatory protein SNF2
sI: SWI/SNF chromatin-remodeling complex subunit SWI1
sJ: Regulator of Ty1 transposition protein 102
sK: Actin-related protein 7
sL: Actin-like protein ARP9
E3: Mediator of RNA polymerase II transcription subunit 5
E6: Mediator of RNA polymerase II transcription subunit 16
T1: Mediator of RNA polymerase II transcription subunit 2
T2: Mediator of RNA polymerase II transcription subunit 3
T3: Mediator of RNA polymerase II transcription subunit 5
T4: Mediator of RNA polymerase II transcription subunit 14
T5: Mediator of RNA polymerase II transcription subunit 15
T6: Mediator of RNA polymerase II transcription subunit 16
t1: Mediator of RNA polymerase II transcription subunit 2
t2: Mediator of RNA polymerase II transcription subunit 3
t3: Mediator of RNA polymerase II transcription subunit 5
t4: Mediator of RNA polymerase II transcription subunit 14
t5: Mediator of RNA polymerase II transcription subunit 15
t6: Mediator of RNA polymerase II transcription subunit 16
hetero molecules


Theoretical massNumber of molelcules
Total (without water)7,771,764178
Polymers7,768,657138
Non-polymers3,10740
Water00
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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General transcription and DNA repair factor IIH helicase subunit ... , 2 types, 4 molecules A0B0A7B7

#1: Protein General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / TFIIH subunit XPD / DNA 5'-3' helicase RAD3 / DNA repair helicase RAD3 / RNA polymerase II ...TFIIH subunit XPD / DNA 5'-3' helicase RAD3 / DNA repair helicase RAD3 / RNA polymerase II transcription factor B subunit RAD3 / TFB subunit RAD3


Mass: 89899.047 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P06839, DNA 5'-3' helicase
#8: Protein General transcription and DNA repair factor IIH helicase subunit XPB / TFIIH subunit XPB / DNA repair helicase RAD25 / RNA polymerase II transcription factor B subunit ...TFIIH subunit XPB / DNA repair helicase RAD25 / RNA polymerase II transcription factor B subunit SSL2 / TFB subunit SSL2 / Suppressor of stem-loop mutation 2


Mass: 95461.664 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q00578, DNA helicase

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General transcription and DNA repair factor IIH subunit ... , 5 types, 10 molecules A1B1A2B2A4B4A5B5A6B6

#2: Protein General transcription and DNA repair factor IIH subunit TFB1 / TFIIH subunit TFB1 / RNA polymerase II transcription factor B 73 kDa subunit / RNA polymerase II ...TFIIH subunit TFB1 / RNA polymerase II transcription factor B 73 kDa subunit / RNA polymerase II transcription factor B p73 subunit / RNA polymerase II transcription factor B subunit 1


Mass: 72993.328 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P32776
#3: Protein General transcription and DNA repair factor IIH subunit TFB2 / TFIIH subunit TFB2 / RNA polymerase II transcription factor B 52 kDa subunit / RNA polymerase II ...TFIIH subunit TFB2 / RNA polymerase II transcription factor B 52 kDa subunit / RNA polymerase II transcription factor B p52 subunit / RNA polymerase II transcription factor B subunit 2


Mass: 58602.312 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q02939
#5: Protein General transcription and DNA repair factor IIH subunit TFB4 / TFIIH subunit TFB4 / RNA polymerase II transcription factor B 34 kDa subunit / RNA polymerase II ...TFIIH subunit TFB4 / RNA polymerase II transcription factor B 34 kDa subunit / RNA polymerase II transcription factor B p34 subunit / RNA polymerase II transcription factor B subunit 4


Mass: 37506.422 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q12004
#6: Protein General transcription and DNA repair factor IIH subunit TFB5 / TFIIH subunit TFB5 / RNA polymerase II transcription factor B subunit 5


Mass: 8243.490 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q3E7C1
#7: Protein General transcription and DNA repair factor IIH subunit SSL1 / TFIIH subunit SSL1 / RNA polymerase II transcription factor B subunit SSL1 / TFB subunit SSL1 / ...TFIIH subunit SSL1 / RNA polymerase II transcription factor B subunit SSL1 / TFB subunit SSL1 / Suppressor of stem-loop protein 1


Mass: 52370.035 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q04673

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Protein , 8 types, 18 molecules A3B3AOBOASBSSBSDsBsDSEsESJsJSKsKSLsL

#4: Protein RNA polymerase II transcription factor B subunit 3 / RNA polymerase II transcription factor B 38 kDa subunit / RNA polymerase II transcription factor B p38 subunit


Mass: 38188.438 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q03290
#25: Protein TATA-box-binding protein / TATA sequence-binding protein / TBP / TATA-binding factor / TATA-box factor / Transcription factor ...TATA sequence-binding protein / TBP / TATA-binding factor / TATA-box factor / Transcription factor D / Transcription initiation factor TFIID TBP subunit


Mass: 27042.275 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: SPT15, BTF1, TBP1, YER148W / Production host: Escherichia coli (E. coli) / References: UniProt: P13393
#28: Protein Transcription elongation factor S-II / DNA strand transfer protein alpha / STP-alpha / DNA strand transferase 1 / Pyrimidine pathway ...DNA strand transfer protein alpha / STP-alpha / DNA strand transferase 1 / Pyrimidine pathway regulatory protein 2


Mass: 34903.551 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: DST1, PPR2, YGL043W / Production host: Escherichia coli (E. coli) / References: UniProt: P07273
#51: Protein
SWI/SNF complex subunit SWI3 / Transcription factor TYE2 / Transcription regulatory protein SWI3


Mass: 93034.164 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P32591
#53: Protein SWI/SNF global transcription activator complex subunit SWP82


Mass: 70366.617 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P43554
#57: Protein Regulator of Ty1 transposition protein 102


Mass: 17817.615 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P53330
#58: Protein Actin-related protein 7 / Actin-like protein ARP7 / Chromatin structure-remodeling complex protein ARP7 / SWI/SNF complex component ARP7


Mass: 53863.016 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q12406
#59: Protein Actin-like protein ARP9 / Chromatin structure-remodeling complex protein ARP9 / SWI/SNF complex component ARP9


Mass: 53131.930 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q05123

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SWI/SNF chromatin-remodeling complex subunit ... , 2 types, 6 molecules A8SFB8sFSIsI

#9: Protein
SWI/SNF chromatin-remodeling complex subunit SNF5 / SWI/SNF complex subunit SNF5 / Transcription factor TYE4 / Transcription regulatory protein SNF5


Mass: 102642.172 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P18480
#56: Protein SWI/SNF chromatin-remodeling complex subunit SWI1 / Regulatory protein GAM3 / SWI/SNF complex subunit SWI1 / Transcription regulatory protein ADR6 / ...Regulatory protein GAM3 / SWI/SNF complex subunit SWI1 / Transcription regulatory protein ADR6 / Transcription regulatory protein SWI1


Mass: 148065.188 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P09547

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Transcription initiation factor ... , 8 types, 16 molecules A9B9AMBMAQBQARBRAUBUAVBVAWBWAXBX

#10: Protein Transcription initiation factor TFIID subunit 14 / Actin non-complementing mutant 1 / Chromosome stability protein 10 / SWI/SNF chromatin-remodeling ...Actin non-complementing mutant 1 / Chromosome stability protein 10 / SWI/SNF chromatin-remodeling complex subunit TAF14 / SWI/SNF complex 29 kDa subunit / SWI/SNF complex subunit TAF14 / TBP-associated factor 14 / TBP-associated factor 30 kDa / Transcription factor G 30 kDa subunit / Transcription initiation factor TFIIF 30 kDa subunit


Mass: 27473.154 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P35189
#23: Protein Transcription initiation factor IIB / General transcription factor TFIIB / Transcription factor E


Mass: 38257.340 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: SUA7, YPR086W, P9513.4 / Production host: Escherichia coli (E. coli) / References: UniProt: P29055
#26: Protein Transcription initiation factor IIF subunit alpha / TFIIF-alpha / TFIIF large subunit / Transcription factor G 105 kDa subunit / P105


Mass: 82320.570 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P41895
#27: Protein Transcription initiation factor IIF subunit beta / TFIIF medium subunit / TFIIF-beta / Transcription factor G 54 kDa subunit


Mass: 46684.492 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P41896
#30: Protein Transcription initiation factor IIA large subunit / TFIIA large subunit / TFIIA 32 kDa subunit


Mass: 32230.805 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: TOA1, YOR194C / Production host: Escherichia coli (E. coli) / References: UniProt: P32773
#31: Protein Transcription initiation factor IIA subunit 2 / General transcription factor IIA subunit 2 / TFIIA 13.5 kDa subunit / Transcription initiation ...General transcription factor IIA subunit 2 / TFIIA 13.5 kDa subunit / Transcription initiation factor IIA small chain / Transcription initiation factor IIA small subunit


Mass: 13473.070 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: TOA2, YKL058W / Production host: Escherichia coli (E. coli) / References: UniProt: P32774
#32: Protein Transcription initiation factor IIE subunit alpha / TFIIE-alpha / Factor A 66 kDa subunit / Transcription factor A large subunit


Mass: 54804.809 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P36100
#33: Protein Transcription initiation factor IIE subunit beta / TFIIE-beta / Factor A 43 kDa subunit / Transcription factor A small subunit


Mass: 37050.434 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P36145

+
DNA-directed RNA polymerase II subunit ... , 7 types, 16 molecules AAMABAmAABBBACBCADBDAGBGAIBIAKBK

#11: Protein
DNA-directed RNA polymerase II subunit RPB1 / RNA polymerase II subunit 1 / RNA polymerase II subunit B1 / DNA-directed RNA polymerase III ...RNA polymerase II subunit 1 / RNA polymerase II subunit B1 / DNA-directed RNA polymerase III largest subunit / RNA polymerase II subunit B220


Mass: 191821.578 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P04050, DNA-directed RNA polymerase
#12: Protein DNA-directed RNA polymerase II subunit RPB2 / RNA polymerase II subunit 2 / B150 / DNA-directed RNA polymerase II 140 kDa polypeptide


Mass: 138937.297 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P08518, DNA-directed RNA polymerase
#13: Protein DNA-directed RNA polymerase II subunit RPB3 / RNA polymerase II subunit 3 / RNA polymerase II subunit B3 / B44.5 / DNA-directed RNA polymerase II ...RNA polymerase II subunit 3 / RNA polymerase II subunit B3 / B44.5 / DNA-directed RNA polymerase II 45 kDa polypeptide


Mass: 35330.457 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P16370
#14: Protein DNA-directed RNA polymerase II subunit RPB4 / RNA polymerase II subunit B4 / B32 / DNA-directed RNA polymerase II 32 kDa polypeptide


Mass: 25451.191 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P20433
#17: Protein DNA-directed RNA polymerase II subunit RPB7 / RNA polymerase II subunit B7 / B16


Mass: 19081.053 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P34087
#19: Protein DNA-directed RNA polymerase II subunit RPB9 / RNA polymerase II subunit B9 / B12.6 / DNA-directed RNA polymerase II 14.2 kDa polypeptide / DNA- ...RNA polymerase II subunit B9 / B12.6 / DNA-directed RNA polymerase II 14.2 kDa polypeptide / DNA-directed RNA polymerase II subunit 9


Mass: 14308.161 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P27999
#21: Protein DNA-directed RNA polymerase II subunit RPB11 / RNA polymerase II subunit B11 / B13.6 / DNA-directed RNA polymerase II 13.6 kDa polypeptide


Mass: 13633.493 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P38902

+
DNA-directed RNA polymerases I, II, and III subunit ... , 5 types, 10 molecules AEBEAFBFAHBHAJBJALBL

#15: Protein DNA-directed RNA polymerases I, II, and III subunit RPABC1 / RNA polymerases I / II / and III subunit ABC1 / ABC27 / DNA-directed RNA polymerases I / and III 27 ...RNA polymerases I / II / and III subunit ABC1 / ABC27 / DNA-directed RNA polymerases I / and III 27 kDa polypeptide


Mass: 25117.094 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P20434
#16: Protein DNA-directed RNA polymerases I, II, and III subunit RPABC2


Mass: 17931.834 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P20435
#18: Protein DNA-directed RNA polymerases I, II, and III subunit RPABC3 / RNA polymerases I / II / and III subunit ABC3 / ABC14.4 / ABC14.5 / DNA-directed RNA polymerases I ...RNA polymerases I / II / and III subunit ABC3 / ABC14.4 / ABC14.5 / DNA-directed RNA polymerases I / and III 14.5 kDa polypeptide


Mass: 16525.363 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P20436
#20: Protein DNA-directed RNA polymerases I, II, and III subunit RPABC5 / RNA polymerases I / II / and III subunit ABC5 / ABC10-beta / ABC8 / DNA-directed RNA polymerases I ...RNA polymerases I / II / and III subunit ABC5 / ABC10-beta / ABC8 / DNA-directed RNA polymerases I / and III 8.3 kDa polypeptide


Mass: 8290.732 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P22139
#22: Protein DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerases I / II / and III subunit ABC4 / ABC10-alpha


Mass: 7729.969 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P40422

+
DNA chain , 2 types, 4 molecules ANBNATBT

#24: DNA chain promoter DNA


Mass: 21252.670 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 173135
#29: DNA chain promoter DNA


Mass: 21279.705 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 536627

+
Mediator of RNA polymerase II transcription subunit ... , 21 types, 46 molecules MamaMbmbMcmcMdmdMemeMfmfMgmgMhmhMimiMjmjMkmkMlmlT4t4MmmmMnmn...

#34: Protein Mediator of RNA polymerase II transcription subunit 6 / Mediator complex subunit 6


Mass: 32844.746 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P38782
#35: Protein Mediator of RNA polymerase II transcription subunit 8 / Mediator complex subunit 8


Mass: 25297.234 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P38304
#36: Protein Mediator of RNA polymerase II transcription subunit 11 / Mediator complex subunit 11


Mass: 13324.189 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q99278
#37: Protein Mediator of RNA polymerase II transcription subunit 17 / Mediator complex subunit 17 / Suppressor of RNA polymerase B 4


Mass: 78582.836 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P32569
#38: Protein Mediator of RNA polymerase II transcription subunit 18 / Mediator complex subunit 18 / Suppressor of RNA polymerase B 5


Mass: 34316.539 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P32585
#39: Protein Mediator of RNA polymerase II transcription subunit 20 / Hyper-recombination suppressor protein 2 / Mediator complex subunit 20 / Suppressor of RNA polymerase B 2


Mass: 22918.068 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P34162
#40: Protein Mediator of RNA polymerase II transcription subunit 22 / Mediator complex subunit 22 / Suppressor of RNA polymerase B 6


Mass: 13875.731 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P32570
#41: Protein Mediator of RNA polymerase II transcription subunit 4 / Mediator complex subunit 4


Mass: 32244.895 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q12343
#42: Protein Mediator of RNA polymerase II transcription subunit 7 / Mediator complex subunit 7


Mass: 25616.062 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q08278
#43: Protein Mediator of RNA polymerase II transcription subunit 9 / Chromosome segregation protein 2 / Mediator complex subunit 9


Mass: 17400.377 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P33308
#44: Protein Mediator of RNA polymerase II transcription subunit 10 / Mediator complex subunit 10 / Negative regulator of URS2 protein 2


Mass: 17927.926 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q06213
#45: Protein
Mediator of RNA polymerase II transcription subunit 14 / Glucose repression regulatory protein 1 / Mediator complex subunit 14


Mass: 123500.695 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P19263
#46: Protein Mediator of RNA polymerase II transcription subunit 19 / Hypoxic gene repressor protein 3 / Mediator complex subunit 19 / Negative regulator of URS2 protein ...Hypoxic gene repressor protein 3 / Mediator complex subunit 19 / Negative regulator of URS2 protein 3 / SNF1 suppressor protein 7


Mass: 24885.305 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P25046
#47: Protein Mediator of RNA polymerase II transcription subunit 21 / Mediator complex subunit 21 / RNAPII complex component SRB7 / Suppressor of RNA polymerase B 7


Mass: 16093.368 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P47822
#48: Protein Mediator of RNA polymerase II transcription subunit 31 / Hpr1 suppressor protein 1 / Mediator complex subunit 31


Mass: 14747.739 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P38633
#49: Protein Mediator of RNA polymerase II transcription subunit 1 / Mediator complex subunit 1


Mass: 64314.953 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q12321
#60: Protein Mediator of RNA polymerase II transcription subunit 5 / Mediator complex subunit 5 / Negative regulator of URS2 protein 1


Mass: 128918.750 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P53114
#61: Protein Mediator of RNA polymerase II transcription subunit 16 / Global transcriptional regulator SIN4 / Mediator complex subunit 16 / SNF1 suppressor protein 4 / ...Global transcriptional regulator SIN4 / Mediator complex subunit 16 / SNF1 suppressor protein 4 / SWI4 suppressor protein 5 / Transcriptional silencing factor 3 / YGP1 expression regulatory protein 1


Mass: 111423.422 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P32259
#62: Protein Mediator of RNA polymerase II transcription subunit 2 / Mediator complex subunit 2


Mass: 47754.270 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q12124
#63: Protein Mediator of RNA polymerase II transcription subunit 3 / Hyper-recombination suppressor protein 1 / Mediator complex subunit 3 / Poly-glutamine domain protein 1


Mass: 43116.039 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P40356
#64: Protein Mediator of RNA polymerase II transcription subunit 15 / Autonomous replication regulatory protein 3 / Basal expression activator protein 1 / Defective ...Autonomous replication regulatory protein 3 / Basal expression activator protein 1 / Defective silencing suppressor protein 4 / Mediator complex subunit 15 / Transcription regulatory protein GAL11 / Ty insertion suppressor protein 13


Mass: 120432.242 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P19659

+
Transcription regulatory protein ... , 3 types, 6 molecules SAsASCsCSHsH

#50: Protein Transcription regulatory protein SNF12 / SWI/SNF complex component SWP73


Mass: 63947.633 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P53628
#52: Protein Transcription regulatory protein SNF6 / SWI/SNF complex component SNF6


Mass: 37652.582 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P18888
#55: Protein Transcription regulatory protein SNF2 / ATP-dependent helicase SNF2 / Regulatory protein GAM1 / Regulatory protein SWI2 / SWI/SNF complex ...ATP-dependent helicase SNF2 / Regulatory protein GAM1 / Regulatory protein SWI2 / SWI/SNF complex component SNF2 / Transcription factor TYE3


Mass: 194315.094 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast)
References: UniProt: P22082, Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement

+
Protein/peptide , 1 types, 2 molecules SGsG

#54: Protein/peptide unsigned peptide of SWISNF


Mass: 4591.546 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast)

+
Non-polymers , 3 types, 40 molecules

#65: Chemical ChemComp-SF4 / IRON/SULFUR CLUSTER


Mass: 351.640 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Fe4S4
#66: Chemical...
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 36 / Source method: obtained synthetically / Formula: Zn
#67: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg

+
Details

Has ligand of interestN
Has protein modificationY

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Open form of PIC-Med-SWI/SNF / Type: COMPLEX / Entity ID: #1-#64 / Source: MULTIPLE SOURCES
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 64000 X / Nominal defocus max: 2500 nm / Nominal defocus min: 1000 nm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingAverage exposure time: 4 sec. / Electron dose: 40.8 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)
EM imaging opticsPhase plate: OTHER

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Processing

EM software
IDNameVersionCategory
1RELION5particle selection
2UCSF ChimeraX1.11/v10model fitting
3SerialEMimage acquisition
13RELION53D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 3090211
Details: this is the composite map , the information above is parameter of the consensus map before focused refinement for each part.
Symmetry type: POINT
Atomic model buildingProtocol: RIGID BODY FIT

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