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- PDB-9og1: Globular domain of monkeypox virus OPG153 (A28) bound to antibody... -

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Basic information

Entry
Database: PDB / ID: 9og1
TitleGlobular domain of monkeypox virus OPG153 (A28) bound to antibody 02M12
Components
  • A28L
  • Monoclonal antibody 02M12 heavy chain
  • Monoclonal antibody 02M12 light chain
KeywordsVIRAL PROTEIN / Monkeypox virus / neutralizing antibody / orthopoxvirus
Function / homologyOrthopoxvirus A26L/A30L / Orthopoxvirus A26L/A30L protein / Chordopoxvirus fusion protein/multifunctional envelope protein A27 / Chordopoxvirus multifunctional envelope protein A27 / fusion of virus membrane with host plasma membrane / viral envelope / A28L
Function and homology information
Biological speciesMonkeypox virus
Homo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsRundlet, E.J. / Zhou, L. / McLellan, J.S.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: Sci Transl Med / Year: 2025
Title: Antigen-agnostic identification of poxvirus broadly neutralizing antibodies targeting OPG153.
Authors: Ida Paciello / Emily J Rundlet / Ling Zhou / Giulia Realini / Federica Perrone / Giulio Pierleoni / Marco Troisi / Jeanne Postal / Florence Guivel-Benhassine / Françoise Porrot / Connor M ...Authors: Ida Paciello / Emily J Rundlet / Ling Zhou / Giulia Realini / Federica Perrone / Giulio Pierleoni / Marco Troisi / Jeanne Postal / Florence Guivel-Benhassine / Françoise Porrot / Connor M Mullins / Davide Moschese / Maria Vittoria Cossu / Federico Sabaini / Silvia Accordini / Natasha Gianesini / Rebeca Passarelli Mantovani / Francesca Panza / Massimiliano Fabbiani / Mario Tumbarello / Spinello Antinori / Concetta Castilletti / Francesca Montagnani / Olivier Schwartz / Rino Rappuoli / Jason S McLellan / Emanuele Andreano /
Abstract: Recurrent mpox outbreaks caused by the monkeypox virus (MPXV) have prompted the World Health Organization to declare a Public Health Emergency of International Concern and have stimulated the ...Recurrent mpox outbreaks caused by the monkeypox virus (MPXV) have prompted the World Health Organization to declare a Public Health Emergency of International Concern and have stimulated the development of medical interventions. Here, we used antigen-agnostic identification of MPXV-neutralizing monoclonal antibodies from convalescent or vaccinated people, AlphaFold 3-based predictive modeling, and cryo-electron microscopy to identify the protein encoded by orthopoxviral gene (OPG) 153 (MPXV A28) as a target of broadly neutralizing antibodies. OPG153-targeting antibodies neutralized MPXV clades Ib and IIb and vaccinia virus (VACV) and cross-reacted with OPG153 orthologs from cowpox and variola viruses. Immunization with MPXV OPG153 elicited a potent neutralizing antibody response against MPXV and VACV in mice, substantiating OPG153 as both a promising vaccine antigen and a potent target for preventive and therapeutic antibodies.
History
DepositionApr 30, 2025Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 24, 2025Provider: repository / Type: Initial release
Revision 1.0Dec 24, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Dec 24, 2025Data content type: Additional map / Part number: 1 / Data content type: Additional map / Provider: repository / Type: Initial release
Revision 1.0Dec 24, 2025Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Dec 24, 2025Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Dec 24, 2025Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Dec 24, 2025Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Dec 24, 2025Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: A28L
B: Monoclonal antibody 02M12 heavy chain
C: Monoclonal antibody 02M12 light chain


Theoretical massNumber of molelcules
Total (without water)74,0373
Polymers74,0373
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

#1: Protein A28L


Mass: 47960.152 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Monkeypox virus / Gene: A28L / Production host: Homo sapiens (human) / References: UniProt: Q8V4V0
#2: Antibody Monoclonal antibody 02M12 heavy chain


Mass: 14123.835 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#3: Antibody Monoclonal antibody 02M12 light chain


Mass: 11953.371 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Monkeypox virus OPG153 (A28) globular domain in complex with antibody 02M12
Type: COMPLEX / Entity ID: all / Source: MULTIPLE SOURCES
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Homo sapiens (human) / Cell: Freestyle 293F
Buffer solutionpH: 8
SpecimenConc.: 0.22 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Details: 1:1 molar ratio of OPG153 (1-366) + nonoclonal antibody 02M12 Fab
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

MicroscopyModel: TFS GLACIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1500 nm
Image recordingElectron dose: 49 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)

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Processing

EM softwareName: PHENIX / Version: 1.21.1_5286: / Category: model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 132110 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0024729
ELECTRON MICROSCOPYf_angle_d0.3966403
ELECTRON MICROSCOPYf_dihedral_angle_d9.3761710
ELECTRON MICROSCOPYf_chiral_restr0.041688
ELECTRON MICROSCOPYf_plane_restr0.003813

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