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- EMDB-70449: Globular domain of monkeypox virus OPG153 (A28) bound to antibody... -

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Basic information

Entry
Database: EMDB / ID: EMD-70449
TitleGlobular domain of monkeypox virus OPG153 (A28) bound to antibody 02M12
Map dataMain map-DeepEMhancer
Sample
  • Complex: Monkeypox virus OPG153 (A28) globular domain in complex with antibody 02M12
    • Protein or peptide: A28L
    • Protein or peptide: Monoclonal antibody 02M12 heavy chain
    • Protein or peptide: Monoclonal antibody 02M12 light chain
KeywordsMonkeypox virus / neutralizing antibody / orthopoxvirus / VIRAL PROTEIN
Function / homologyOrthopoxvirus A26L/A30L / Orthopoxvirus A26L/A30L protein / Chordopoxvirus fusion protein/multifunctional envelope protein A27 / Chordopoxvirus multifunctional envelope protein A27 / fusion of virus membrane with host plasma membrane / viral envelope / A28L
Function and homology information
Biological speciesHomo sapiens (human) / Monkeypox virus
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsRundlet EJ / Zhou L / McLellan JS
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Sci Transl Med / Year: 2025
Title: Antigen-agnostic identification of poxvirus broadly neutralizing antibodies targeting OPG153.
Authors: Ida Paciello / Emily J Rundlet / Ling Zhou / Giulia Realini / Federica Perrone / Giulio Pierleoni / Marco Troisi / Jeanne Postal / Florence Guivel-Benhassine / Françoise Porrot / Connor M ...Authors: Ida Paciello / Emily J Rundlet / Ling Zhou / Giulia Realini / Federica Perrone / Giulio Pierleoni / Marco Troisi / Jeanne Postal / Florence Guivel-Benhassine / Françoise Porrot / Connor M Mullins / Davide Moschese / Maria Vittoria Cossu / Federico Sabaini / Silvia Accordini / Natasha Gianesini / Rebeca Passarelli Mantovani / Francesca Panza / Massimiliano Fabbiani / Mario Tumbarello / Spinello Antinori / Concetta Castilletti / Francesca Montagnani / Olivier Schwartz / Rino Rappuoli / Jason S McLellan / Emanuele Andreano /
Abstract: Recurrent mpox outbreaks caused by the monkeypox virus (MPXV) have prompted the World Health Organization to declare a Public Health Emergency of International Concern and have stimulated the ...Recurrent mpox outbreaks caused by the monkeypox virus (MPXV) have prompted the World Health Organization to declare a Public Health Emergency of International Concern and have stimulated the development of medical interventions. Here, we used antigen-agnostic identification of MPXV-neutralizing monoclonal antibodies from convalescent or vaccinated people, AlphaFold 3-based predictive modeling, and cryo-electron microscopy to identify the protein encoded by orthopoxviral gene (OPG) 153 (MPXV A28) as a target of broadly neutralizing antibodies. OPG153-targeting antibodies neutralized MPXV clades Ib and IIb and vaccinia virus (VACV) and cross-reacted with OPG153 orthologs from cowpox and variola viruses. Immunization with MPXV OPG153 elicited a potent neutralizing antibody response against MPXV and VACV in mice, substantiating OPG153 as both a promising vaccine antigen and a potent target for preventive and therapeutic antibodies.
History
DepositionApr 30, 2025-
Header (metadata) releaseDec 24, 2025-
Map releaseDec 24, 2025-
UpdateDec 24, 2025-
Current statusDec 24, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70449.png

Downloads & links

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Map

FileDownload / File: emd_70449.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMain map-DeepEMhancer
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 300 pix.
= 279.9 Å		0.93 Å/pix.
x 300 pix.
= 279.9 Å		0.93 Å/pix.
x 300 pix.
= 279.9 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.933 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.08
Minimum - Maximum-0.049828578 - 1.7585167
Average (Standard dev.)0.0006346417 (±0.018388623)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 279.9 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Non-uniform refinement map, sharpened

Fileemd_70449_additional_1.map
AnnotationNon-uniform refinement map, sharpened
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map A

Fileemd_70449_half_map_1.map
AnnotationHalf map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map B

Fileemd_70449_half_map_2.map
AnnotationHalf map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Monkeypox virus OPG153 (A28) globular domain in complex with anti...

EntireName: Monkeypox virus OPG153 (A28) globular domain in complex with antibody 02M12
Components
  • Complex: Monkeypox virus OPG153 (A28) globular domain in complex with antibody 02M12
    • Protein or peptide: A28L
    • Protein or peptide: Monoclonal antibody 02M12 heavy chain
    • Protein or peptide: Monoclonal antibody 02M12 light chain

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Supramolecule #1: Monkeypox virus OPG153 (A28) globular domain in complex with anti...

SupramoleculeName: Monkeypox virus OPG153 (A28) globular domain in complex with antibody 02M12
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: A28L

MacromoleculeName: A28L / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Monkeypox virus
Molecular weightTheoretical: 47.960152 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MANIINLWNG IVPTVQDVNV ASITAFKSMI DETWDKKIEA NTCISRKHRN IIHEVIRDFM KAYPKMDENR KSPLGAPMQW LTQYYILKN EYHKTMLAYD DGSLNTKFKT LNIYMITNVG QYILYIVFCI ISGKNHDGTP YIYDSEITSN DKNLINDRIK Y ACKQILHG ...String:
MANIINLWNG IVPTVQDVNV ASITAFKSMI DETWDKKIEA NTCISRKHRN IIHEVIRDFM KAYPKMDENR KSPLGAPMQW LTQYYILKN EYHKTMLAYD DGSLNTKFKT LNIYMITNVG QYILYIVFCI ISGKNHDGTP YIYDSEITSN DKNLINDRIK Y ACKQILHG QLTMALRIRN KFMFIGSPMY LWFNVNGSHV YHEIYDRNVG FHNKEIGRLL YAFMYYLSIS GRFLNDLALL KF TYLGESW TFSLSVPEYI LYGLGYSVFD TIEKFSNDAI LVYIRTNNRN GYDYAEFNKK GIVKVTEDKP DNDKRIHAIR LIN YSSDVQ HIHFGFRNTL IIDNECTSIQ SSAENATDTG HHQDSKINIE GSLEVLFQGP GHHHHHHHHS AWSHPQFEKG GASG GGGSG GSAWSNPQFE K

UniProtKB: A28L

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Macromolecule #2: Monoclonal antibody 02M12 heavy chain

MacromoleculeName: Monoclonal antibody 02M12 heavy chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.123835 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
(PCA)VQLVQSGAE VKKPGASVKV SCKASGYTFT GYYMHWVRQA PGQGLEWMGW INPNTGGTNY AQKFQGRVTM TRDTSI NTA YMDLSRLRSD DTAVYYCARG WRYSYLDCFD PWGQGTLVIV SSASTKGP

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Macromolecule #3: Monoclonal antibody 02M12 light chain

MacromoleculeName: Monoclonal antibody 02M12 light chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.953371 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
AIQMTQSPSS VSASVGDRVT ITCRASQGIS IWLAWYQQKP GKAPKLLINA ASSLQSGVPS RFSGSGSGTD FTLTISSLQP EDFATYYCQ QANSFPYTFG QGTKLEIKRT VAA

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.22 mg/mL
BufferpH: 8
VitrificationCryogen name: ETHANE
Details1:1 molar ratio of OPG153 (1-366) + nonoclonal antibody 02M12 Fab

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 49.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER / Details: Alphafold 3
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 132110
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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