+ Open data
Open data
- Basic information
Basic information
| Entry | Database: PDB / ID: 9n74 | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Title | SSU processome maturation and disassembly, State H | |||||||||||||||
|  Components | 
 | |||||||||||||||
|  Keywords | RIBOSOME / SSU processome / ribosome assembly / RNA folding / RNA-protein interactions | |||||||||||||||
| Function / homology |  Function and homology information 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / nuclear polyadenylation-dependent mRNA catabolic process / regulation of ribosomal protein gene transcription by RNA polymerase II / U1 snRNA 3'-end processing / rRNA small subunit pseudouridine methyltransferase Nep1 / box H/ACA snoRNA binding ...18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / nuclear polyadenylation-dependent mRNA catabolic process / regulation of ribosomal protein gene transcription by RNA polymerase II / U1 snRNA 3'-end processing / rRNA small subunit pseudouridine methyltransferase Nep1 / box H/ACA snoRNA binding / nuclear polyadenylation-dependent CUT catabolic process / RNA fragment catabolic process / U5 snRNA 3'-end processing / Noc4p-Nop14p complex / rRNA 2'-O-methylation / TRAMP-dependent tRNA surveillance pathway / exosome (RNase complex) / CURI complex / UTP-C complex / U4 snRNA 3'-end processing / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / t-UTP complex / nuclear polyadenylation-dependent rRNA catabolic process / poly(A)-dependent snoRNA 3'-end processing / nuclear microtubule / snoRNA guided rRNA 2'-O-methylation / Pwp2p-containing subcomplex of 90S preribosome / Mpp10 complex / nuclear exosome (RNase complex) / rRNA (pseudouridine) methyltransferase activity / rRNA modification / exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / histone H2AQ104 methyltransferase activity / box C/D sno(s)RNA binding / regulation of rRNA processing / septum digestion after cytokinesis / tRNA re-export from nucleus / snRNA binding / rRNA (adenine-N6,N6-)-dimethyltransferase activity / histone mRNA catabolic process / RNA folding chaperone / box C/D sno(s)RNA 3'-end processing / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA methyltransferase activity / rDNA heterochromatin / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear mRNA surveillance / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / regulation of transcription by RNA polymerase I / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / box C/D methylation guide snoRNP complex / U4/U6 snRNP / positive regulation of rRNA processing / tRNA export from nucleus / single-stranded telomeric DNA binding / rRNA primary transcript binding / sno(s)RNA-containing ribonucleoprotein complex / Negative regulators of DDX58/IFIH1 signaling / rRNA base methylation / U4 snRNA binding / O-methyltransferase activity / SUMOylation of RNA binding proteins / protein localization to nucleolus / 90S preribosome assembly / mTORC1-mediated signalling / rRNA methylation / Protein hydroxylation / RNA catabolic process / U4 snRNP / regulation of telomere maintenance / poly(U) RNA binding / U3 snoRNA binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / poly(A)+ mRNA export from nucleus / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / snoRNA binding / preribosome, small subunit precursor / precatalytic spliceosome / Major pathway of rRNA processing in the nucleolus and cytosol / establishment of cell polarity / spliceosomal complex assembly / positive regulation of transcription by RNA polymerase I / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / nucleolar large rRNA transcription by RNA polymerase I / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / 90S preribosome / Ub-specific processing proteases / proteasome assembly / ribosomal subunit export from nucleus / RNA processing / regulation of translational fidelity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / U4/U6 x U5 tri-snRNP complex Similarity search - Function | |||||||||||||||
| Biological species |   Saccharomyces cerevisiae S288C (yeast) | |||||||||||||||
| Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.65 Å | |||||||||||||||
|  Authors | Buzovetsky, O. / Klinge, S. | |||||||||||||||
| Funding support |  United States, 2items 
 | |||||||||||||||
|  Citation |  Journal: To Be Published Title: Mechanism of helicase-mediated SSU processome maturation and disassembly Authors: Buzovetsky, O. / Klinge, S. | |||||||||||||||
| History | 
 | 
- Structure visualization
Structure visualization
| Structure viewer | Molecule:  Molmil  Jmol/JSmol | 
|---|
- Downloads & links
Downloads & links
- Download
Download
| PDBx/mmCIF format |  9n74.cif.gz | 5.3 MB | Display |  PDBx/mmCIF format | 
|---|---|---|---|---|
| PDB format |  pdb9n74.ent.gz | Display |  PDB format | |
| PDBx/mmJSON format |  9n74.json.gz | Tree view |  PDBx/mmJSON format | |
| Others |  Other downloads | 
-Validation report
| Summary document |  9n74_validation.pdf.gz | 1.8 MB | Display |  wwPDB validaton report | 
|---|---|---|---|---|
| Full document |  9n74_full_validation.pdf.gz | 1.9 MB | Display | |
| Data in XML |  9n74_validation.xml.gz | 554.3 KB | Display | |
| Data in CIF |  9n74_validation.cif.gz | 947.7 KB | Display | |
| Arichive directory |  https://data.pdbj.org/pub/pdb/validation_reports/n7/9n74  ftp://data.pdbj.org/pub/pdb/validation_reports/n7/9n74 | HTTPS FTP | 
-Related structure data
| Related structure data |  49084MC  9n73C  9n7bC  48993  48994  48995  48996  48997  48998  48999  49000  49001 M: map data used to model this data C: citing same article ( | 
|---|---|
| Similar structure data | Similarity search - Function & homology  F&H Search | 
- Links
Links
- Assembly
Assembly
| Deposited unit |  
 | 
|---|---|
| 1 | 
 | 
- Components
Components
+RNA chain , 3 types, 3 molecules L0L1L2  
+40S ribosomal protein  ... , 18 types, 18 molecules L3L4L5L6L7L8L9LCLDLELFLGNFNGNPNQOHSR                 
+Protein , 19 types, 22 molecules LHLOLUNBNDNLNMNSNVOAOUSCSDSESFSHSISJSKSVSWSZ                     
+U3 small nucleolar RNA-associated protein  ... , 17 types, 17 molecules LILJLKLLLMLNLPLQLRLSLTLWNANHSPSSSY                
+U3 small nucleolar ribonucleoprotein protein  ... , 2 types, 2 molecules LZSM 
+Ribosomal RNA-processing protein  ... , 2 types, 2 molecules NISG 
+Nucleolar protein  ... , 2 types, 2 molecules SASB 
+RRNA-processing protein  ... , 2 types, 2 molecules SLSQ 
+Nucleolar complex protein  ... , 2 types, 2 molecules STSU 
+Protein/peptide , 1 types, 1 molecules SX
+Non-polymers , 5 types, 45 molecules 








+Details
-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY | 
|---|---|
| EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction | 
- Sample preparation
Sample preparation
| Component | Name: SSU processome maturation and disassembly, State H / Type: COMPLEX / Entity ID: #1-#68 / Source: NATURAL | ||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Source (natural) | Organism:   Saccharomyces cerevisiae (brewer's yeast) | ||||||||||||||||||||||||||||
| Buffer solution | pH: 7.5 | ||||||||||||||||||||||||||||
| Buffer component | 
 | ||||||||||||||||||||||||||||
| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||||||||||||||||||
| Vitrification | Cryogen name: ETHANE | 
- Electron microscopy imaging
Electron microscopy imaging
| Experimental equipment |  Model: Titan Krios / Image courtesy: FEI Company | 
|---|---|
| Microscopy | Model: TFS KRIOS | 
| Electron gun | Electron source:  FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER | 
| Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 25000 nm / Nominal defocus min: 1000 nm | 
| Image recording | Electron dose: 61.6 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) | 
- Processing
Processing
| CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | 
|---|---|
| 3D reconstruction | Resolution: 2.65 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 328554 / Symmetry type: POINT | 
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