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- PDB-9mvv: Pfs230 (D9-D14) with Pfs48/45 of the endogenous Pfs230-Pfs48/45 c... -

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Basic information

Entry
Database: PDB / ID: 9mvv
TitlePfs230 (D9-D14) with Pfs48/45 of the endogenous Pfs230-Pfs48/45 complex
Components
  • Gametocyte surface protein P230
  • Gametocyte surface protein P45/48
KeywordsPROTEIN BINDING / 6-cysteine protein / Plasmodium falciparum / malaria transmission
Function / homology
Function and homology information


side of membrane / cell surface / plasma membrane
Similarity search - Function
: / 6-Cysteine (6-Cys) domain / 6-Cysteine (6-Cys) domain superfamily / Sexual stage antigen s48/45 domain / 6-Cysteine (6-Cys) domain profile. / Sexual stage antigen s48/45 domain
Similarity search - Domain/homology
Gametocyte surface protein P230 / Gametocyte surface protein P45/48
Similarity search - Component
Biological speciesPlasmodium falciparum (malaria parasite P. falciparum)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.36 Å
AuthorsDietrich, M.H. / Glukhova, A. / Shakeel, S. / Tham, W.H.
Funding support Australia, 2items
OrganizationGrant numberCountry
National Health and Medical Research Council (NHMRC, Australia)APP2001385 Australia
National Health and Medical Research Council (NHMRC, Australia)APP2016908 Australia
CitationJournal: To Be Published
Title: Cryo-EM structure of the native Pfs230 and Pfs48/45 fertilization complex from Plasmodium falciparum
Authors: Dietrich, M.H. / Chmielewski, J. / Chan, L.J. / Tan, L.L. / Adair, A. / Lyons, F.M.T. / Gabriela, M. / Lopaticki, S. / Dite, T.A. / Dagley, L.F. / Longley, R. / Mueller, I. / Glukhova, A. / ...Authors: Dietrich, M.H. / Chmielewski, J. / Chan, L.J. / Tan, L.L. / Adair, A. / Lyons, F.M.T. / Gabriela, M. / Lopaticki, S. / Dite, T.A. / Dagley, L.F. / Longley, R. / Mueller, I. / Glukhova, A. / Shakeel, S. / Tham, W.H.
History
DepositionJan 16, 2025Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 30, 2025Provider: repository / Type: Initial release
Revision 1.0Jul 30, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Jul 30, 2025Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Jul 30, 2025Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Gametocyte surface protein P230
C: Gametocyte surface protein P45/48


Theoretical massNumber of molelcules
Total (without water)412,2082
Polymers412,2082
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

#1: Protein Gametocyte surface protein P230


Mass: 363701.750 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Plasmodium falciparum (malaria parasite P. falciparum)
References: UniProt: P68874
#2: Protein Gametocyte surface protein P45/48


Mass: 48506.062 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Plasmodium falciparum (malaria parasite P. falciparum)
References: UniProt: Q8I6T1
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Heterodimeric complex of endogenous Plasmodium falciparum proteins Pfs230 and Pfs48/45
Type: COMPLEX / Entity ID: all / Source: NATURAL
Molecular weightValue: 0.4 MDa / Experimental value: NO
Source (natural)Organism: Plasmodium falciparum (malaria parasite P. falciparum)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: GOLD / Grid type: UltrAuFoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 %

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 1600 nm / Nominal defocus min: 400 nm / Cs: 2.7 mm / C2 aperture diameter: 70 µm
Specimen holderCryogen: NITROGEN
Image recordingElectron dose: 50 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)

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Processing

EM software
IDNameCategory
1cryoSPARCparticle selection
4cryoSPARCCTF correction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.36 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 87061 / Symmetry type: POINT
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 104.34 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.003510668
ELECTRON MICROSCOPYf_angle_d0.754114529
ELECTRON MICROSCOPYf_chiral_restr0.05561707
ELECTRON MICROSCOPYf_plane_restr0.00581855
ELECTRON MICROSCOPYf_dihedral_angle_d5.65611459

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