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- EMDB-48672: Consensus map of the endogenous Pfs230-Pfs48/45 complex -

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Basic information

Entry
Database: EMDB / ID: EMD-48672
TitleConsensus map of the endogenous Pfs230-Pfs48/45 complex
Map data
Sample
  • Complex: Heterodimeric complex of endogenous Plasmodium falciparum proteins Pfs230 and Pfs48/45
Keywords6-cysteine protein / Plasmodium falciparum / malaria transmission / PROTEIN BINDING
Biological speciesPlasmodium falciparum (malaria parasite P. falciparum)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.36 Å
AuthorsDietrich MH / Glukhova A / Shakeel S / Tham WH
Funding support Australia, 2 items
OrganizationGrant numberCountry
National Health and Medical Research Council (NHMRC, Australia)APP2001385 Australia
National Health and Medical Research Council (NHMRC, Australia)APP2016908 Australia
CitationJournal: Science / Year: 2025
Title: Cryo-EM structure of endogenous Pfs230 and Pfs48/45 fertilization complex.
Authors: Melanie H Dietrich / Jill Chmielewski / Li-Jin Chan / Li Lynn Tan / Amy Adair / Frankie M T Lyons / Mikha Gabriela / Sash Lopaticki / Toby A Dite / Laura F Dagley / Lucia Pazzagli / Priya ...Authors: Melanie H Dietrich / Jill Chmielewski / Li-Jin Chan / Li Lynn Tan / Amy Adair / Frankie M T Lyons / Mikha Gabriela / Sash Lopaticki / Toby A Dite / Laura F Dagley / Lucia Pazzagli / Priya Gupta / Mohd Kamil / Ashley M Vaughan / Rattanaporn Rojrung / Anju Abraham / Ramin Mazhari / Rhea J Longley / Kathleen Zeglinski / Quentin Gouil / Ivo Mueller / Stewart A Fabb / Rekha Shandre-Mugan / Colin W Pouton / Alisa Glukhova / Shabih Shakeel / Wai-Hong Tham /
Abstract: Malaria parasite fertilization occurs in the midgut of a female mosquito. Blocking fertilization within the mosquito can prevent malaria transmission. Pfs230 and Pfs48/45 proteins are critical for ...Malaria parasite fertilization occurs in the midgut of a female mosquito. Blocking fertilization within the mosquito can prevent malaria transmission. Pfs230 and Pfs48/45 proteins are critical for male fertility and transmission of the malaria parasite. They form a core fertilization complex, but it is unknown how they interact. We determined a cryo-electron microscopy structure of the endogenous Pfs230-Pfs48/45 complex showing that Pfs48/45 interacts with Pfs230 domains 13 and 14. Transgenic parasite lines with these domains removed were defective in Pfs230 gamete localization and showed reduced oocyst formation. Nanobodies against domains 13 and 14 inhibited Pfs230-Pfs48/45 complex formation and reduced transmission, and structural analyses revealed their epitopes. These Pfs230 domains were targets of naturally acquired immunity and immune sera from messenger RNA lipid nanoparticle immunizations blocked parasite transmission.
History
DepositionJan 16, 2025-
Header (metadata) releaseAug 6, 2025-
Map releaseAug 6, 2025-
UpdateSep 24, 2025-
Current statusSep 24, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_48672.png

Downloads & links

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Map

FileDownload / File: emd_48672.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.81 Å/pix.
x 360 pix.
= 290.88 Å		0.81 Å/pix.
x 360 pix.
= 290.88 Å		0.81 Å/pix.
x 360 pix.
= 290.88 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.808 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0489
Minimum - Maximum-0.119806446 - 14.227244000000001
Average (Standard dev.)-0.024382327 (±0.4046718)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 290.88 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_48672_additional_1.map
Projections & Slices
AxesZYX

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Half map: #1

Fileemd_48672_half_map_1.map
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Half map: #2

Fileemd_48672_half_map_2.map
Projections & Slices
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Histogram (log scale)

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Sample components

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Entire : Heterodimeric complex of endogenous Plasmodium falciparum protein...

EntireName: Heterodimeric complex of endogenous Plasmodium falciparum proteins Pfs230 and Pfs48/45
Components
  • Complex: Heterodimeric complex of endogenous Plasmodium falciparum proteins Pfs230 and Pfs48/45

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Supramolecule #1: Heterodimeric complex of endogenous Plasmodium falciparum protein...

SupramoleculeName: Heterodimeric complex of endogenous Plasmodium falciparum proteins Pfs230 and Pfs48/45
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Source (natural)Organism: Plasmodium falciparum (malaria parasite P. falciparum)
Molecular weightTheoretical: 400 KDa

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.6 µm / Nominal defocus min: 0.4 µm
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.36 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 87061
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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