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- PDB-9lju: Structural insights into the polymerase catalyzed FAD-capping of ... -

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Basic information

Entry
Database: PDB / ID: 9lju
TitleStructural insights into the polymerase catalyzed FAD-capping of hepatitis C viral RNA
Components
  • RNA (5'-R(P*CP*CP*U)-3')
  • RNA-directed RNA polymerase
KeywordsVIRAL PROTEIN / hepatitis C virus / RNA polymerase complex / FAD cap
Function / homology
Function and homology information


hepacivirin / host cell mitochondrial membrane / host cell lipid droplet / symbiont-mediated transformation of host cell / symbiont-mediated suppression of host TRAF-mediated signal transduction / Dectin-2 family / symbiont-mediated perturbation of host cell cycle G1/S transition checkpoint / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / SH3 domain binding ...hepacivirin / host cell mitochondrial membrane / host cell lipid droplet / symbiont-mediated transformation of host cell / symbiont-mediated suppression of host TRAF-mediated signal transduction / Dectin-2 family / symbiont-mediated perturbation of host cell cycle G1/S transition checkpoint / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / SH3 domain binding / nucleoside-triphosphate phosphatase / channel activity / viral nucleocapsid / monoatomic ion transmembrane transport / clathrin-dependent endocytosis of virus by host cell / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / RNA helicase activity / host cell perinuclear region of cytoplasm / host cell endoplasmic reticulum membrane / RNA helicase / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / ribonucleoprotein complex / symbiont-mediated activation of host autophagy / serine-type endopeptidase activity / RNA-directed RNA polymerase / cysteine-type endopeptidase activity / viral RNA genome replication / RNA-directed RNA polymerase activity / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell nucleus / host cell plasma membrane / virion membrane / structural molecule activity / ATP hydrolysis activity / proteolysis / RNA binding / zinc ion binding / ATP binding / identical protein binding / membrane
Similarity search - Function
Hepatitus C virus, Non-structural 5a protein, C-terminal / Hepatitis C virus NS5A, 1B domain superfamily / Hepatitis C virus non-structural protein NS2, C-terminal domain / Hepatitis C virus non-structural protein NS2, N-terminal domain / Hepatitis C virus non-structural protein NS2 / HCV NS5a protein C-terminal region / Hepatitis C virus, Non-structural protein NS4b / Hepatitis C virus, Core protein, N-terminal / Hepatitis C virus core protein, chain A superfamily / : ...Hepatitus C virus, Non-structural 5a protein, C-terminal / Hepatitis C virus NS5A, 1B domain superfamily / Hepatitis C virus non-structural protein NS2, C-terminal domain / Hepatitis C virus non-structural protein NS2, N-terminal domain / Hepatitis C virus non-structural protein NS2 / HCV NS5a protein C-terminal region / Hepatitis C virus, Non-structural protein NS4b / Hepatitis C virus, Core protein, N-terminal / Hepatitis C virus core protein, chain A superfamily / : / Hepatitis C virus non-structural protein NS4b / Hepatitis C virus capsid protein / Hepatitis C virus, Non-structural protein NS2 / Hepatitis C virus, Non-structural 5a protein / Hepatitis C virus, Non-structural 5a protein, domain 1a / Hepatitis C virus non-structural 5a, 1B domain / NS5A domain 1a superfamily / : / Hepatitis C virus non-structural 5a protein membrane anchor / Hepatitis C virus non-structural 5a zinc finger domain / Hepatitis C virus non-structural 5a domain 1b / NS3 RNA helicase, C-terminal helical domain / Hepacivirus nonstructural protein 2 (NS2) protease domain profile. / Hepatitis C virus, Non-structural protein NS4a / Hepatitis C virus non-structural protein NS4a / Hepatitis C virus, Core protein, C-terminal / Hepatitis C virus core protein / Hepatitis C virus, Non-structural protein E2/NS1 / Hepatitis C virus non-structural protein E2/NS1 / Hepatitis C virus, Envelope glycoprotein E1 / Hepatitis C virus envelope glycoprotein E1 / RNA dependent RNA polymerase, hepatitis C virus / Viral RNA dependent RNA polymerase / Hepatitis C virus, NS3 protease, Peptidase S29 / Hepatitis C virus NS3 protease / Hepacivirus/Pegivirus NS3 protease domain profile. / DEAD box, Flavivirus / Flavivirus DEAD domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / Reverse transcriptase/Diguanylate cyclase domain / RNA-directed RNA polymerase, catalytic domain / RdRp of positive ssRNA viruses catalytic domain profile. / Peptidase S1, PA clan, chymotrypsin-like fold / Peptidase S1, PA clan / DNA/RNA polymerase superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
FLAVIN-ADENINE DINUCLEOTIDE / GUANOSINE-5'-DIPHOSPHATE / : / RNA / Genome polyprotein
Similarity search - Component
Biological speciesHepatitis C virus JFH-1
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.92 Å
AuthorsWang, D.P. / Zhao, R. / Hu, W.S. / Li, H.N. / Cao, J.M. / Zhou, X. / Xiang, Y.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)2401055, 31925023, 21827810, 31861143027 China
CitationJournal: Nat Commun / Year: 2025
Title: Structural insights into polymerase-catalyzed FAD capping of hepatitis C virus RNA.
Authors: Wang, D.P. / Zhao, R. / Hu, W.S. / Li, H.N. / Cao, J.M. / Zhou, X. / Xiang, Y.
History
DepositionJan 15, 2025Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Aug 13, 2025Provider: repository / Type: Initial release
Revision 1.1Aug 20, 2025Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: RNA-directed RNA polymerase
T: RNA (5'-R(P*CP*CP*U)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)64,1016
Polymers62,7632
Non-polymers1,3394
Water1,08160
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3210 Å2
ΔGint-36 kcal/mol
Surface area23400 Å2
MethodPISA
Unit cell
Length a, b, c (Å)139.313, 139.313, 93.140
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number170
Space group name H-MP65

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Components

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Protein / RNA chain , 2 types, 2 molecules AT

#1: Protein RNA-directed RNA polymerase / NS5B / p68


Mass: 61891.078 Da / Num. of mol.: 1 / Mutation: S15G/E86Q/E87Q/C223H/V321I
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Hepatitis C virus JFH-1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q99IB8, RNA-directed RNA polymerase
#2: RNA chain RNA (5'-R(P*CP*CP*U)-3')


Mass: 871.572 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Non-polymers , 4 types, 64 molecules

#3: Chemical ChemComp-FAD / FLAVIN-ADENINE DINUCLEOTIDE


Mass: 785.550 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C27H33N9O15P2 / Comment: FAD*YM
#4: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#5: Chemical ChemComp-MN / MANGANESE (II) ION


Mass: 54.938 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mn
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 60 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.16 Å3/Da / Density % sol: 70.41 %
Crystal growTemperature: 289.15 K / Method: vapor diffusion, sitting drop
Details: PEG 3350, 100 mM Bis-Tris propane/hydrochloric acid, pH 8.5, 200 mM Sodium malonate dibasic precipitant

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Data collection

DiffractionMean temperature: 289.15 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL10U2 / Wavelength: 0.97918 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Dec 24, 2023
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 2.74→93.02 Å / Num. obs: 26742 / % possible obs: 98.7 % / Redundancy: 10.8 % / CC1/2: 0.986 / Net I/σ(I): 8.8
Reflection shellResolution: 2.74→2.89 Å / Num. unique obs: 3635 / CC1/2: 0.45

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Processing

Software
NameVersionClassification
PHENIX(1.21.2_5419)refinement
XDSdata scaling
XDSdata reduction
PHENIXphasing
PDB_EXTRACTdata extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.92→21.63 Å / SU ML: 0.37 / Cross valid method: THROUGHOUT / Phase error: 23.17 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2161 3827 -
Rwork0.1738 --
obs-22228 98.88 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.92→21.63 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4341 60 83 60 4544
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0094604
X-RAY DIFFRACTIONf_angle_d1.0626279
X-RAY DIFFRACTIONf_dihedral_angle_d17.4381714
X-RAY DIFFRACTIONf_chiral_restr0.051706
X-RAY DIFFRACTIONf_plane_restr0.009776
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.92-2.960.34451380.29921414X-RAY DIFFRACTION96
2.96-30.28191520.29071428X-RAY DIFFRACTION95
3-3.040.34851360.27251422X-RAY DIFFRACTION98
3.04-3.080.29821400.26641480X-RAY DIFFRACTION98
3.08-3.130.2871400.2431419X-RAY DIFFRACTION98
3.13-3.180.2691450.24471481X-RAY DIFFRACTION100
3.18-3.230.33291520.24161468X-RAY DIFFRACTION98
3.23-3.280.27691380.2251470X-RAY DIFFRACTION100
3.28-3.340.28521300.22681454X-RAY DIFFRACTION99
3.34-3.410.3241380.21681466X-RAY DIFFRACTION100
3.41-3.480.27361480.21561461X-RAY DIFFRACTION99
3.48-3.550.24031420.18811465X-RAY DIFFRACTION99
3.55-3.640.22871420.19011442X-RAY DIFFRACTION99
3.64-3.730.29361320.17851505X-RAY DIFFRACTION99
3.73-3.830.20161460.16451447X-RAY DIFFRACTION100
3.83-3.940.21961420.16071447X-RAY DIFFRACTION99
3.94-4.060.19891460.14941472X-RAY DIFFRACTION99
4.07-4.210.18351330.14491458X-RAY DIFFRACTION98
4.21-4.380.16791470.13191464X-RAY DIFFRACTION99
4.38-4.570.1791500.12891434X-RAY DIFFRACTION100
4.57-4.810.17661500.13681504X-RAY DIFFRACTION100
4.81-5.110.17491440.13461439X-RAY DIFFRACTION99
5.11-5.50.17651390.14911473X-RAY DIFFRACTION100
5.5-6.040.18541360.16251478X-RAY DIFFRACTION100
6.04-6.890.23361400.16641473X-RAY DIFFRACTION100
6.89-8.580.14851470.13931480X-RAY DIFFRACTION100
8.6-21.630.14181480.12611415X-RAY DIFFRACTION97

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