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- PDB-9leq: paralysed flagellum protein PflA N-terminal domain and PflB complex -

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Basic information

Entry
Database: PDB / ID: 9leq
Titleparalysed flagellum protein PflA N-terminal domain and PflB complex
Components
  • Flagellar protein
  • Motility protein PflB
KeywordsSTRUCTURAL PROTEIN / paralysed flagellum protein
Function / homology
Function and homology information


: / Domain of unknown function (DUF7494) / : / Tetratricopeptide repeat / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Tetratricopeptide-like helical domain superfamily
Similarity search - Domain/homology
Flagellar protein / Motility protein PflB
Similarity search - Component
Biological speciesCampylobacter jejuni subsp. jejuni serotype O:23/36 (Campylobacter)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.23 Å
AuthorsHe, J. / Gao, X.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: Nat Microbiol / Year: 2025
Title: The architecture, assembly, and evolution of a complex flagellar motor
Authors: He, J. / Gao, X.
History
DepositionJan 7, 2025Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Oct 15, 2025Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Flagellar protein
B: Motility protein PflB


Theoretical massNumber of molelcules
Total (without water)90,3302
Polymers90,3302
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein Flagellar protein


Mass: 16950.250 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: The sequence of organism Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) is not available during the biocuration, replaced by A0A5Z4HSG8 temporarily.
Source: (gene. exp.) Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) (Campylobacter)
Gene: B9I30_07525, CNS88_00980 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A5Z4HSG8
#2: Protein Motility protein PflB


Mass: 73380.180 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: The sequence of organism Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) is not available during the biocuration, replaced by A0A6F9L7Y3 temporarily.
Source: (gene. exp.) Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) (Campylobacter)
Gene: pflB, FVY41_04030 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A6F9L7Y3
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Campylobacter jejuni flagellum protein PflA N-terminal domain and PflB
Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Source (natural)Organism: Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) (Campylobacter)
Source (recombinant)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

MicroscopyModel: TFS GLACIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1200 nm
Image recordingElectron dose: 40 e/Å2 / Film or detector model: TFS FALCON 4i (4k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.23 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 182785 / Symmetry type: POINT
RefinementCross valid method: NONE

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