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- EMDB-63032: paralysed flagellum protein PflA N-terminal domain and PflB complex -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-63032
Titleparalysed flagellum protein PflA N-terminal domain and PflB complex
Map data
Sample
  • Complex: Campylobacter jejuni flagellum protein PflA N-terminal domain and PflB
    • Protein or peptide: Flagellar protein
    • Protein or peptide: Motility protein PflB
Keywordsparalysed flagellum protein / STRUCTURAL PROTEIN
Function / homology
Function and homology information


: / Domain of unknown function (DUF7494) / : / Tetratricopeptide repeat / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Tetratricopeptide-like helical domain superfamily
Similarity search - Domain/homology
Flagellar protein / Motility protein PflB
Similarity search - Component
Biological speciesCampylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) (Campylobacter)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.23 Å
AuthorsHe J / Gao X
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Nat Microbiol / Year: 2025
Title: The architecture, assembly, and evolution of a complex flagellar motor
Authors: He J / Gao X
History
DepositionJan 7, 2025-
Header (metadata) releaseOct 15, 2025-
Map releaseOct 15, 2025-
UpdateOct 15, 2025-
Current statusOct 15, 2025Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_63032.png

Downloads & links

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Map

FileDownload / File: emd_63032.map.gz / Format: CCP4 / Size: 40.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.89 Å/pix.
x 220 pix.
= 195.8 Å		0.89 Å/pix.
x 220 pix.
= 195.8 Å		0.89 Å/pix.
x 220 pix.
= 195.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.89 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.32
Minimum - Maximum-1.7856119 - 2.3232207
Average (Standard dev.)0.0011759669 (±0.062143527)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions220220220
Spacing220220220
CellA=B=C: 195.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_63032_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_63032_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Campylobacter jejuni flagellum protein PflA N-terminal domain and PflB

EntireName: Campylobacter jejuni flagellum protein PflA N-terminal domain and PflB
Components
  • Complex: Campylobacter jejuni flagellum protein PflA N-terminal domain and PflB
    • Protein or peptide: Flagellar protein
    • Protein or peptide: Motility protein PflB

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Supramolecule #1: Campylobacter jejuni flagellum protein PflA N-terminal domain and PflB

SupramoleculeName: Campylobacter jejuni flagellum protein PflA N-terminal domain and PflB
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) (Campylobacter)

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Macromolecule #1: Flagellar protein

MacromoleculeName: Flagellar protein / type: protein_or_peptide / ID: 1
Details: The sequence of organism Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) is not available during the biocuration, replaced by A0A5Z4HSG8 temporarily.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) (Campylobacter)
Molecular weightTheoretical: 16.95025 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MAFELVLNTG RENNQAFAVL HASNDLEFTC QKFITEAKVH FECDIVGMVD NKLKDQSFSA FDLKFIQEAQ KIKMIILPKI QARMFDTSQ NIYIDKELSS SSSHKSKAFT FIFTPELAPI KDYDGLDFNI NFPHESLPYV GALDLNSDPV

UniProtKB: Flagellar protein

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Macromolecule #2: Motility protein PflB

MacromoleculeName: Motility protein PflB / type: protein_or_peptide / ID: 2
Details: The sequence of organism Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) is not available during the biocuration, replaced by A0A6F9L7Y3 temporarily.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) (Campylobacter)
Molecular weightTheoretical: 73.38018 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: LSNYNLGVSQ MNENKFQEAF ESFKKAIANG ENQSVAAINA AVCALKLNDK EKFRYYIDLA QVYLPKEGKS KLYDYYLSLI NYYKGYYPE ALQMLQRTDM EPYSDVAKYL SAKIYAKMDF DSKAIQQLNS QGNFESSLSL GLLYARIGEY AKAKVALSTA M KIERDFNQ ...String:
LSNYNLGVSQ MNENKFQEAF ESFKKAIANG ENQSVAAINA AVCALKLNDK EKFRYYIDLA QVYLPKEGKS KLYDYYLSLI NYYKGYYPE ALQMLQRTDM EPYSDVAKYL SAKIYAKMDF DSKAIQQLNS QGNFESSLSL GLLYARIGEY AKAKVALSTA M KIERDFNQ SLAALTLVDI KTGNYQDMLA RLQNSYDNDK DRYKILDAYK IQVRLNKDLF NIAVAQNSFS KDLLKKQKDQ FD LLFYFAP YQVFDSKQAA LYIKKANVTD FVDDSADAGV YLNTSKALSS TNVKIANIIN YALNQKLRLA NQEFQKLIKD YPE HSILHY NLALTYAQMQ NYELAYKHFS SSYHLNPKNY LAGAFAMFCA KLIDIDTTKL YNEILDNIAA DSNFKANMQK SMLF LVNNN YISMLPYLDE TKKDTPLSLI FEAIVAKNNN LNNQVDVKIA KLRSELPEDI LANILYFNSL NSNLNIKEYA QNAQI HFKN LKLDYRSVFG GPNIAREFYV NLMHIAGLLN LERQKIKELI NVSGAKDEGI LQTLAYLDIF AQQYEEAYAL YNSLID DYG AKDTKTLFLA AVAAIGANNP NSAIALLQLS KLTDKNNKES KAALGMLYQE VKNYEAAISQ YKTLPNNFKS EFFTFDI NN N

UniProtKB: Motility protein PflB

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.23 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 182785
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
FSC plot (resolution estimation)

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