PDB-9k7c: Crystal structure of GH57 family amylopullulanase from Aquifex ae...

ID or keywords:

Entry

Database: PDB / ID: 9k7c

Title

Crystal structure of GH57 family amylopullulanase from Aquifex aeolicus mutant E256Q in complex with maltopentaose

Components

Glycoside hydrolase family 57 N-terminal domain-containing protein

Keywords

HYDROLASE / GH57 / amylopullulanase / Aquifex aeolicus

Function / homology

: / Glycoside hydrolase family 57, N-terminal domain / Glycosyl hydrolase family 57 / Glycoside hydrolase 38, N-terminal domain superfamily / Glycoside hydrolase/deacetylase, beta/alpha-barrel / catalytic activity / carbohydrate metabolic process / alpha-maltotriose / Glycoside hydrolase family 57 N-terminal domain-containing protein

Function and homology information

Biological species

Aquifex aeolicus VF5 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.85 Å

Authors

Zhu, Z.M. / Wang, W.W. / Li, M.J. / Xu, Q. / Zhou, H. / Huang, L.Q. / Wang, Q.S. / Yu, F.

Funding support

1items

Citation

Journal: Commun Biol / Year: 2025
Title: The crystal structure of GH57 family amylopullulanase reveals its dual binding pockets sharing the same catalytic dyad.
Authors: Zhu, Z. / Wang, W. / Li, M. / Xu, Q. / Zhou, H. / Huang, L. / Wang, Q. / Yu, F.

History

Deposition	Oct 23, 2024	Deposition site: PDBJ / Processing site: PDBC
Revision 1.0	Jun 4, 2025	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	9k7c.cif.gz	813.5 KB	Display	PDBx/mmCIF format
PDB format	pdb9k7c.ent.gz	680.9 KB	Display	PDB format
PDBx/mmJSON format	9k7c.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	9k7c_validation.pdf.gz	2.6 MB	Display	wwPDB validaton report
Full document	9k7c_full_validation.pdf.gz	2.6 MB	Display
Data in XML	9k7c_validation.xml.gz	90.8 KB	Display
Data in CIF	9k7c_validation.cif.gz	120.8 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/k7/9k7c ftp://data.pdbj.org/pub/pdb/validation_reports/k7/9k7c	HTTPS FTP

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Related structure data

Related structure data	8zyiC 9ihtC 9ihuC 9ihvC 9ihwC 9ihxC 9ii0C 9ii1C 9k7dC C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#203 - Nov 2016 Aminopeptidase 1 and Autophagy similarity (2)

Deposited unit

A: Glycoside hydrolase family 57 N-terminal domain-containing protein

B: Glycoside hydrolase family 57 N-terminal domain-containing protein

C: Glycoside hydrolase family 57 N-terminal domain-containing protein

D: Glycoside hydrolase family 57 N-terminal domain-containing protein

hetero molecules

234 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Glycoside hydrolase family 57 N-terminal domain-containing protein

hetero molecules

defined by author&software
Evidence: gel filtration
58.7 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Glycoside hydrolase family 57 N-terminal domain-containing protein

hetero molecules

defined by author&software
58.7 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

C: Glycoside hydrolase family 57 N-terminal domain-containing protein

hetero molecules

defined by author&software
58.1 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

D: Glycoside hydrolase family 57 N-terminal domain-containing protein

hetero molecules

defined by author&software
58.6 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	40.820, 61.373, 195.564
Angle α, β, γ (deg.)	83.98, 89.08, 89.18
Int Tables number	1
Space group name H-M	P1

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Protein , 1 types, 4 molecules ABCD

#1: Protein	Glycoside hydrolase family 57 N-terminal domain-containing protein Mass: 57252.250 Da / Num. of mol.: 4 / Mutation: E256Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aquifex aeolicus VF5 (bacteria) / Gene: aq_720 / Production host: Escherichia coli (E. coli) / References: UniProt: O66934

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Sugars , 3 types, 7 molecules

#2: Polysaccharide

alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose

Type: oligosaccharide, Oligosaccharide / Class: Nutrient / Mass: 504.438 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: oligosaccharide / References: alpha-maltotriose

Descriptor	Type	Program
DGlcpa1-4DGlcpa1-4DGlcpa1-ROH	Glycam Condensed Sequence	GMML 1.0
WURCS=2.0/1,3,2/[a2122h-1a_1-5]/1-1-1/a4-b1_b4-c1	WURCS	PDB2Glycan 1.1.0
[][a-D-Glcp]{[(4+1)][a-D-Glcp]{[(4+1)][a-D-Glcp]{}}}	LINUCS	PDB-CARE

#3: Polysaccharide

alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D- ...

Type: oligosaccharide / Mass: 828.719 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source

Descriptor	Type	Program
DGlcpa1-4DGlcpa1-4DGlcpa1-4DGlcpa1-4DGlcpa1-ROH	Glycam Condensed Sequence	GMML 1.0
WURCS=2.0/1,5,4/[a2122h-1a_1-5]/1-1-1-1-1/a4-b1_b4-c1_c4-d1_d4-e1	WURCS	PDB2Glycan 1.1.0
[][a-D-Glcp]{[(4+1)][a-D-Glcp]{[(4+1)][a-D-Glcp]{[(4+1)][a-D-Glcp]{[(4+1)][a-D-Glcp]{}}}}}	LINUCS	PDB-CARE

#4: Polysaccharide

alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose

Type: oligosaccharide / Mass: 666.578 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source

Descriptor	Type	Program
DGlcpa1-4DGlcpa1-4DGlcpa1-4DGlcpa1-ROH	Glycam Condensed Sequence	GMML 1.0
WURCS=2.0/1,4,3/[a2122h-1a_1-5]/1-1-1-1/a4-b1_b4-c1_c4-d1	WURCS	PDB2Glycan 1.1.0
[][a-D-Glcp]{[(4+1)][a-D-Glcp]{[(4+1)][a-D-Glcp]{[(4+1)][a-D-Glcp]{}}}}	LINUCS	PDB-CARE

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Non-polymers , 2 types, 1008 molecules

#5: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₃H₈O₃
#6: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 1007 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y
Has protein modification	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.09 Å³/Da / Density % sol: 41.07 %
Crystal grow	Temperature: 338 K / Method: vapor diffusion, sitting drop / Details: 0.1 M Sodium citrate, pH 4.2, 20% PEG 3350

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SSRF / Beamline: BL02U1 / Wavelength: 0.97918 Å
Detector	Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Oct 17, 2024
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.97918 Å / Relative weight: 1
Reflection	Resolution: 1.85→194.46 Å / Num. obs: 157091 / % possible obs: 97.5 % / Redundancy: 1.9 % / CC_1/2: 0.982 / R_merge(I) obs: 0.12 / R_pim(I) all: 0.117 / R_rim(I) all: 0.167 / Χ²: 0.36 / Net I/σ(I): 2.7 / Num. measured all: 292110
Reflection shell	Resolution: 1.85→1.95 Å / % possible obs: 96.2 % / Redundancy: 1.8 % / R_merge(I) obs: 0.729 / Num. measured all: 41962 / Num. unique obs: 22720 / CC_1/2: 0.43 / R_pim(I) all: 0.713 / R_rim(I) all: 1.021 / Χ²: 0.31 / Net I/σ(I) obs: 0.9

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Processing

Software

Name	Version	Classification
PHENIX	(1.13_2998: ???)	refinement
Aimless		data scaling
XDS		data reduction
PHENIX		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 1.85→34.312 Å / SU ML: 0.24 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 23.29 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2258	7651	4.91 %
R_work	0.1888	-	-
obs	0.1906	155761	96.67 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 1.85→34.312 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	16487	0	40	1007	17534

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.004	17011
X-RAY DIFFRACTION	f_angle_d	0.76	23053
X-RAY DIFFRACTION	f_dihedral_angle_d	5.775	11169
X-RAY DIFFRACTION	f_chiral_restr	0.047	2488
X-RAY DIFFRACTION	f_plane_restr	0.004	2851

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.85-1.8709	0.3046	238	0.2714	4303	X-RAY DIFFRACTION	85
1.8709-1.8929	0.3296	270	0.2832	4488	X-RAY DIFFRACTION	89
1.8929-1.916	0.3439	229	0.2797	4821	X-RAY DIFFRACTION	94
1.916-1.9403	0.322	220	0.2801	4853	X-RAY DIFFRACTION	94
1.9403-1.9658	0.283	250	0.2665	4854	X-RAY DIFFRACTION	96
1.9658-1.9927	0.2904	230	0.2556	4916	X-RAY DIFFRACTION	96
1.9927-2.0212	0.3008	257	0.2436	4977	X-RAY DIFFRACTION	96
2.0212-2.0514	0.2892	275	0.2335	4961	X-RAY DIFFRACTION	97
2.0514-2.0834	0.2641	240	0.2218	4861	X-RAY DIFFRACTION	97
2.0834-2.1176	0.2638	252	0.2182	5042	X-RAY DIFFRACTION	97
2.1176-2.1541	0.2895	258	0.2182	5023	X-RAY DIFFRACTION	97
2.1541-2.1932	0.2585	256	0.2187	4871	X-RAY DIFFRACTION	97
2.1932-2.2354	0.265	225	0.2189	5021	X-RAY DIFFRACTION	97
2.2354-2.281	0.2632	254	0.211	4974	X-RAY DIFFRACTION	97
2.281-2.3306	0.2432	274	0.2002	4857	X-RAY DIFFRACTION	97
2.3306-2.3848	0.2529	266	0.2075	5074	X-RAY DIFFRACTION	98
2.3848-2.4444	0.2475	253	0.2039	4907	X-RAY DIFFRACTION	97
2.4444-2.5105	0.2393	246	0.1918	4981	X-RAY DIFFRACTION	98
2.5105-2.5844	0.2453	244	0.1903	5010	X-RAY DIFFRACTION	98
2.5844-2.6677	0.226	282	0.183	5027	X-RAY DIFFRACTION	98
2.6677-2.7631	0.2182	257	0.1785	5005	X-RAY DIFFRACTION	98
2.7631-2.8736	0.2234	245	0.1793	5000	X-RAY DIFFRACTION	98
2.8736-3.0043	0.2347	246	0.1762	4995	X-RAY DIFFRACTION	98
3.0043-3.1626	0.2194	246	0.1839	5051	X-RAY DIFFRACTION	98
3.1626-3.3606	0.199	242	0.1761	5057	X-RAY DIFFRACTION	99
3.3606-3.6198	0.2037	313	0.1607	4985	X-RAY DIFFRACTION	99
3.6198-3.9836	0.1781	256	0.1493	5016	X-RAY DIFFRACTION	99
3.9836-4.5589	0.1521	241	0.1377	5123	X-RAY DIFFRACTION	99
4.5589-5.7394	0.1722	297	0.1508	5025	X-RAY DIFFRACTION	99
5.7394-34.312	0.2001	289	0.1784	5032	X-RAY DIFFRACTION	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.5026	0.0142	0.2765	0.8921	0.0965	1.0631	0.0451	-0.0006	-0.0012	-0.1539	-0.0704	0.185	0.2286	-0.1013	-0.0037	0.009	-0.0252	0.0115	0.0825	-0.0038	0.12	-24.1772	-33.7018	26.1726
2	0.5382	0.4993	0.4446	1.5889	0.0964	1.3186	-0.0805	0.164	-0.1519	-0.5773	0.2484	-0.3487	-0.0411	0.282	-0.0002	0.1702	0.0175	0.0506	0.1394	-0.0214	0.1698	-11.1184	-44.8676	9.6328
3	0.5242	-0.003	-0.5612	0.7756	0.3911	1.6171	-0.0811	-0.0508	-0.1118	0.0022	0.1332	0.0702	0.4343	0.1127	0.0854	0.0754	-0.0225	-0.0222	0.0508	0.0078	0.1595	-21.2326	-48.2936	23.0047
4	0.0868	-0.1093	-0.0515	0.5944	0.0092	0.5051	-0.1951	-0.0413	0.1022	-0.4583	-0.0615	0.2017	-1.1842	-0.1877	-0.3799	-0.6565	0.0041	0.0381	0.0983	0.0071	0.1674	-26.4449	-14.0201	29.5053
5	0.6031	-0.1886	0.1899	0.4191	0.0095	0.2314	-0.0279	-0.0318	0.0863	0.6374	0.0164	-0.1819	-0.7489	0.1618	-0.0132	-0.4867	-0.0451	0.0217	0.1012	-0.0185	0.1658	-11.8124	-17.1609	40.096
6	0.5019	0.153	-0.0517	1.4911	0.5351	0.9564	0.0419	-0.0905	0.0191	0.3966	0.0571	-0.1926	0.2054	0.014	0.0193	0.05	-0.0115	-0.0308	0.1027	0.0027	0.1205	-11.8775	-30.4035	44.0712
7	0.248	0.0168	-0.0999	0.8781	0.117	1.0798	-0.006	-0.022	0.0966	-0.176	-0.0377	-0.1541	-0.2074	0.0974	-0.0291	0.0754	-0.0175	-0.0099	0.0861	0.0018	0.1288	-17.6584	-10.1603	21.4695
8	1.1654	-0.2763	-0.1277	1.6296	-0.0303	1.4271	-0.0188	0.0556	0.0329	-0.3015	-0.0334	0.0461	0.0463	0.0825	-0.0005	0.1328	-0.0061	-0.023	0.0915	0.006	0.0985	-23.3595	-20.3391	11.6901
9	0.5228	-0.046	0.0289	1.0604	-0.0127	0.3301	-0.0067	-0.0142	0.0093	0.2253	0.051	0.099	-0.032	-0.1733	-0.0096	0.1135	0.0065	0.031	0.1347	0.0082	0.1091	-4.6951	-47.2964	73.4158
10	0.2029	-0.253	-0.3276	0.6839	0.0781	1.3093	0.0547	-0.1818	-0.0918	0.8281	0.004	-0.6346	-0.1707	0.4107	0.0084	0.5811	-0.0234	-0.1461	0.2852	-0.0144	0.056	8.4721	-38.5164	91.6716
11	0.3283	-0.0686	0.2389	0.8083	-0.0131	0.9164	-0.0406	-0.0579	0.1108	0.2991	0.0366	0.0548	-0.3561	-0.0421	0.0069	0.2858	0.014	0.0391	0.1601	-0.0039	0.1541	-2.2239	-33.3025	79.4942
12	0.5946	-0.3135	0.0338	0.6558	0.236	0.7477	-0.0303	0.014	-0.0786	0.099	0.0182	0.0903	0.1392	-0.143	-0.0057	0.0844	-0.0235	0.0226	0.1456	0.0222	0.141	-6.9135	-66.2384	67.447
13	0.6422	0.7145	0.0216	1.1078	-0.2179	0.4913	-0.0446	-0.0548	-0.05	-0.2413	-0.0259	-0.1828	0.0754	0.0247	-0.0263	0.1003	0.0141	0.0233	0.1313	-0.0095	0.1791	7.592	-61.0549	57.3471
14	0.5666	0.0589	0.2627	0.8218	0.1855	1.1793	0.0304	0.0466	0.0402	-0.1378	0.0043	-0.1762	0.0338	0.2062	-0.0415	0.0831	0.0089	0.0356	0.1428	0.0188	0.1243	10.9457	-49.2396	57.1668
15	0.7192	-0.2362	-0.3624	0.9441	-0.2798	0.874	-0.0113	0.0933	0.0057	-0.188	-0.0005	-0.0432	-0.0072	-0.0012	-0.0181	0.0698	0.0061	0.0148	0.1048	0.0276	0.1252	3.3307	-45.4839	53.7043
16	0.738	-0.0256	0.0483	0.8497	-0.0122	1.6003	-0.0574	-0.1402	-0.0907	0.2112	0.0061	-0.1725	0.299	0.2529	0.0213	0.2111	0.0124	-0.0143	0.1304	0.0053	0.1737	2.2293	-71.1065	74.4948
17	1.0045	-0.0466	-0.2878	1.7569	-0.045	1.6788	0.0378	-0.0947	-0.028	0.5824	0.0541	-0.0601	0.008	0.0001	-0.0128	0.2786	0.014	0.0485	0.1611	0.0145	0.0959	-3.5774	-62.7972	85.8906
18	0.4215	-0.1171	0.2113	0.7582	0.2236	0.4401	0.0285	0.0131	0.0305	-0.0002	-0.073	0.0628	-0.0574	-0.0815	0.0549	0.2063	0.0112	0.0025	0.112	0.0058	0.0981	-28.0386	-29.8559	-21.5567
19	0.2685	-0.2346	-0.1463	0.5956	0.238	1.1737	-0.0513	-0.0049	0.094	0.1338	0.111	-0.3651	0.1469	0.2485	-0.0088	0.192	-0.0034	-0.02	0.2049	-0.0123	0.2173	-10.4664	-21.0586	-10.7472
20	0.5398	0.0616	0.4141	0.9088	0.4811	0.7668	0.0019	0.0255	0.1231	-0.1575	-0.04	0.0144	-0.1946	-0.0267	0.0223	0.2653	0.0322	0.0104	0.1098	0.0073	0.1489	-24.5574	-16.5338	-20.6521
21	0.3264	0.0093	0.0005	0.7063	-0.0493	0.9082	0.0285	0.0538	-0.0501	-0.1583	-0.0261	0.0782	0.1903	-0.0721	0.024	0.234	0.0279	-0.0278	0.1464	-0.0166	0.143	-27.7113	-49.7385	-30.3272
22	0.4995	-0.2413	-0.31	0.7899	0.2295	1.1059	0.1535	0.2192	0.0297	-0.2585	-0.0802	-0.0459	-0.0694	0.0458	-0.0223	0.3091	0.0659	0.0155	0.2061	0.0142	0.116	-19.6553	-34.6607	-43.0155
23	0.7431	0.0522	-0.0085	0.8213	-0.039	1.1465	0.0221	0.0502	-0.0446	0.048	-0.0217	-0.0413	0.1186	0.0634	0.0245	0.25	0.011	-0.0097	0.0968	-0.0062	0.1353	-21.9738	-54.7261	-19.7926
24	1.3207	0.2198	0.1598	1.8175	0.0902	1.4282	-0.0253	0.0169	-0.0342	0.2676	-0.0129	0.0085	-0.0514	0.0598	0.0291	0.2299	0.012	0.0057	0.1105	0.006	0.0989	-25.5547	-44.4321	-9.1432
25	0.5	-0.16	-0.1072	1.3686	0.1954	0.7987	-0.1109	0.0631	-0.0181	-0.0682	-0.0144	0.2239	0.1554	-0.0925	0.0405	0.2289	-0.044	-0.0263	0.18	-0.0019	0.1515	-9.4869	-17.2906	-71.5966
26	0.2319	-0.2275	-0.1639	1.8711	0.6561	1.3222	-0.0116	0.0431	-0.0161	-0.2297	0.0147	0.092	0.0595	0.0036	0.0207	0.2056	-0.0371	-0.0177	0.1849	0.0019	0.1669	-5.0618	-18.2822	-77.1205
27	0.3161	-0.1759	-0.0565	1.5303	0.1585	1.1217	-0.0915	-0.1208	-0.0021	0.4444	0.2009	-0.1073	0.0603	0.1332	-0.084	0.2582	0.0243	-0.0524	0.1961	-0.03	0.1599	-2.0026	-8.432	-54.1064
28	0.3463	0.0683	-0.0914	1.1567	0.2431	1.4933	0.0995	0.156	0.1319	-0.9167	0.1025	-0.334	-0.3181	-0.0809	0.0002	0.5911	-0.0159	0.0368	0.1893	0.0288	0.1469	-4.6496	1.4978	-80.8156

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 2 through 81 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 82 through 123 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 124 through 185 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 186 through 278 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 279 through 322 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 323 through 396 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 397 through 435 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 436 through 477 )
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 1 through 81 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 82 through 123 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 124 through 185 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 186 through 278 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 279 through 322 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 323 through 360 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 361 through 396 )
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 397 through 435 )
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 436 through 477 )
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 2 through 88 )
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 89 through 123 )
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 124 through 185 )
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 186 through 322 )
22	X-RAY DIFFRACTION	22	chain 'C' and (resid 323 through 396 )
23	X-RAY DIFFRACTION	23	chain 'C' and (resid 397 through 435 )
24	X-RAY DIFFRACTION	24	chain 'C' and (resid 436 through 477 )
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 2 through 81 )
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 82 through 245 )
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 246 through 405 )
28	X-RAY DIFFRACTION	28	chain 'D' and (resid 406 through 477 )

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