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- PDB-9ija: A local Cryo-EM structure of Bitter taste receptor TAS2R14 with G... -
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Open data
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Basic information
Entry | Database: PDB / ID: 9ija | ||||||
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Title | A local Cryo-EM structure of Bitter taste receptor TAS2R14 with Gi complex | ||||||
![]() | Taste receptor type 2 member 14 | ||||||
![]() | MEMBRANE PROTEIN / GPCR | ||||||
Function / homology | ![]() bitter taste receptor activity / taste receptor activity / detection of chemical stimulus involved in sensory perception of bitter taste / Class C/3 (Metabotropic glutamate/pheromone receptors) / G protein-coupled receptor activity / Sensory perception of sweet, bitter, and umami (glutamate) taste / G alpha (i) signalling events / G protein-coupled receptor signaling pathway / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.05 Å | ||||||
![]() | Yuan, Q. / Duan, J. / Tao, L. / Xu, E.H. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Bitter taste receptor TAS2R14 activation and G protein assembly by an intracellular agonist. Authors: Lele Tao / Dongxue Wang / Qingning Yuan / Fenghui Zhao / Yu Zhang / Tianyuan Du / Shiyi Shen / H Eric Xu / Yi Li / Dehua Yang / Jia Duan / ![]() | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.9 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 23 KB | Display | |
Data in CIF | ![]() | 30.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 60627MC ![]() 9iiwC ![]() 9iixC ![]() 9ij9C M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 36200.070 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-A1AEI / Mass: 321.261 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C13H10F3N7 / Feature type: SUBJECT OF INVESTIGATION |
#3: Chemical | ChemComp-CLR / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: the receptor of TAS2R14 / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7.04 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 18000 nm / Nominal defocus min: 8000 nm / Cs: 2.7 mm |
Image recording | Electron dose: 15 e/Å2 / Film or detector model: GATAN K3 BIOCONTINUUM (6k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 3.05 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 559285 / Symmetry type: POINT |