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- PDB-9i3m: Csu pilus rod antiparallel pair -

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Basic information

Entry
Database: PDB / ID: 9i3m
TitleCsu pilus rod antiparallel pair
ComponentsCsuA/B
KeywordsCELL ADHESION / pili / fimbriae / Acinetobacter baumannii pili / chaperone-usher pathway / archaic chaperone-usher pili / biofilm / 3D biofilm / adhesion / pathogenesis / pilus antiparallel binding junction
Function / homology: / Spore coat protein U / Spore Coat Protein U domain / Spore Coat Protein U domain / Spore coat protein U/FanG domain-containing protein
Function and homology information
Biological speciesAcinetobacter baumannii (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 8.91 Å
AuthorsMalmi, H. / Pakharukova, N. / Zavialov, A.V.
Funding support Finland, 3items
OrganizationGrant numberCountry
Academy of Finland321762 Finland
Academy of Finland360760 Finland
Sigrid Juselius Foundation2023 Finland
CitationJournal: To Be Published
Title: Antiparallel stacking of Csu pili drives Acinetobacter baumannii 3D biofilm assembly
Authors: Malmi, H. / Pakharukova, N. / Paul, B. / Tuittila, M. / Ahmad, I. / Knight, S.D. / Uhlin, B.E. / Ghosal, D. / Zavialov, A.V.
History
DepositionJan 23, 2025Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 17, 2025Provider: repository / Type: Initial release
Revision 1.0Dec 17, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Dec 17, 2025Data content type: Additional map / Part number: 1 / Data content type: Additional map / Provider: repository / Type: Initial release
Revision 1.0Dec 17, 2025Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Dec 17, 2025Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Dec 17, 2025Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Dec 17, 2025Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Dec 17, 2025Data content type: Mask / Part number: 1 / Data content type: Mask / Provider: repository / Type: Initial release
Revision 1.0Dec 17, 2025Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A1: CsuA/B
B1: CsuA/B
C1: CsuA/B
D1: CsuA/B
E1: CsuA/B
F1: CsuA/B
G1: CsuA/B
H1: CsuA/B
I1: CsuA/B
J1: CsuA/B
K1: CsuA/B
L1: CsuA/B
M1: CsuA/B
A2: CsuA/B
B2: CsuA/B
C2: CsuA/B
D2: CsuA/B
E2: CsuA/B
F2: CsuA/B
G2: CsuA/B
H2: CsuA/B
I2: CsuA/B
J2: CsuA/B
K2: CsuA/B
L2: CsuA/B
M2: CsuA/B


Theoretical massNumber of molelcules
Total (without water)417,81126
Polymers417,81126
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

#1: Protein ...
CsuA/B


Mass: 16069.642 Da / Num. of mol.: 26
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Acinetobacter baumannii (bacteria) / Strain: 19096 / Gene: ATCC19606_12570 / Plasmid: pBAD-Csu / Cell (production host): Planktonic bacteria / Production host: Escherichia coli BL21 (bacteria) / Variant (production host): AI / References: UniProt: A0A6F8TDQ5
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Csu pilus rod antiparallel pair / Type: COMPLEX
Details: Csu pilus rods are homopolymers of subunit CsuA/B. The sample contains Csu pilus rods self-assembled into pairs through antiparallel interactions.
Entity ID: all / Source: RECOMBINANT
Molecular weightValue: 16.05974 kDa/nm / Experimental value: NO
Source (natural)Organism: Acinetobacter baumannii (bacteria) / Strain: 19096 / Cellular location: Outer membrane / Organelle: Outer membrane / Tissue: Planktonic bacteria
Source (recombinant)Organism: Escherichia coli (E. coli) / Strain: BL21-AI / Plasmid: pBAD-Csu
Buffer solutionpH: 7.4
Details: Sample is a fraction taken from an ion exchange column elution gradient, so the NaCl concentration may vary.
Buffer component
IDConc.NameFormulaBuffer-ID
120 mMHEPESC8H18N2O4S1
2170 mMsodium chlorideNaCl1
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Details: Csu pilus rods are homopolymers of subunit CsuA/B. The sample contains Csu pilus rods self-assembled into pairs through antiparallel interactions. The pairs and stacks accumulate over time ...Details: Csu pilus rods are homopolymers of subunit CsuA/B. The sample contains Csu pilus rods self-assembled into pairs through antiparallel interactions. The pairs and stacks accumulate over time in a sample tube containing purified Csu pili.
Specimen supportGrid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277.15 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 105000 X / Nominal defocus max: 2500 nm / Nominal defocus min: 800 nm
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingElectron dose: 50 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of grids imaged: 1

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Processing

EM software
IDNameVersionCategoryDetails
1RELION3particle selectionManual picking.
4RELION3CTF correctionCTFFind-4.1 Micrographs with CTF fit better than 3.5 A were used for filament picking.
7UCSF Chimera1.15model fittingUCSF Chimera was used to fit the subunits into the map and adjust their relative angles, and it was also used to adjust map pixel spacing to match model dimensions.
8Coot0.8.9.2 ELmodel fittingCoot was used to adjust side chain and loop positions.
10cryoSPARCv4.5.3initial Euler assignmentAb Initio Reconstruction
11cryoSPARCv4.5.3final Euler assignment
12cryoSPARCv4.5.3classification2D Classification
13cryoSPARCv4.5.33D reconstructionHomogeneous Refinement
14PHENIX1.20.1_4487:model refinementPhenix was used to fit the pair model into the map.
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNum. of particles selected: 35938
3D reconstructionResolution: 8.91 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 12883 / Symmetry type: POINT
Atomic model buildingB value: 304.76 / Protocol: RIGID BODY FIT / Space: REAL
Details: UCSF Chimera was used to first fit the subunits into the single native pilus rod map (PDB Accession ID: 9I37) and average their relative angles, and it was also used to adjust pixel spacing ...Details: UCSF Chimera was used to first fit the subunits into the single native pilus rod map (PDB Accession ID: 9I37) and average their relative angles, and it was also used to adjust pixel spacing of all maps to match model dimensions. Coot was used to adjust side chain and loop positions. A pair of antiparallel pili with subunit angles adjusted to 3-turns-per-7-subunits symmetry was overlapped with a 2D class to adjust the binding junction angle. Finally, Phenix was used to fit the pair model into the map.
Atomic model buildingPDB-ID: 7ZL4

7zl4


Accession code: 7ZL4 / Chain residue range: 24-178
Details: The initial model consisted of a single pilus rod fragment of the same type. The initial model in turn is based on a crystal structure of CsuA/Bsc with PDB accession code 6FM5.
Pdb chain residue range: 24-178 / Source name: PDB / Type: experimental model

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