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- PDB-9frw: Yeast 20S proteasome with human beta1i (1-51) -

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Basic information

Entry
Database: PDB / ID: 9frw
TitleYeast 20S proteasome with human beta1i (1-51)
Components
  • (Proteasome subunit alpha type- ...) x 6
  • (Proteasome subunit beta type- ...) x 7
  • Probable proteasome subunit alpha type-7
KeywordsHYDROLASE / Proteasome / Mutant / Human Chimeric Subunit / Protein Engineering
Function / homology
Function and homology information


spermatoproteasome complex / Proteasome assembly / proteasome core complex / proteasome core complex assembly / nuclear outer membrane-endoplasmic reticulum membrane network / Proteasome assembly / Cross-presentation of soluble exogenous antigens (endosomes) / TNFR2 non-canonical NF-kB pathway / Ubiquitin-Mediated Degradation of Phosphorylated Cdc25A / proteasomal ubiquitin-independent protein catabolic process ...spermatoproteasome complex / Proteasome assembly / proteasome core complex / proteasome core complex assembly / nuclear outer membrane-endoplasmic reticulum membrane network / Proteasome assembly / Cross-presentation of soluble exogenous antigens (endosomes) / TNFR2 non-canonical NF-kB pathway / Ubiquitin-Mediated Degradation of Phosphorylated Cdc25A / proteasomal ubiquitin-independent protein catabolic process / Regulation of PTEN stability and activity / CDK-mediated phosphorylation and removal of Cdc6 / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / KEAP1-NFE2L2 pathway / Neddylation / Orc1 removal from chromatin / MAPK6/MAPK4 signaling / proteasome storage granule / Antigen processing: Ubiquitination & Proteasome degradation / immune system process / proteasome endopeptidase complex / proteasome core complex, beta-subunit complex / endopeptidase activator activity / Ub-specific processing proteases / proteasome assembly / threonine-type endopeptidase activity / proteasome core complex, alpha-subunit complex / Neutrophil degranulation / proteasome complex / peroxisome / endopeptidase activity / proteasome-mediated ubiquitin-dependent protein catabolic process / mRNA binding / endoplasmic reticulum membrane / mitochondrion / extracellular exosome / nucleus / cytosol
Similarity search - Function
Proteasome beta subunit, C-terminal / Proteasome beta subunits C terminal / Proteasome subunit beta 4 / Proteasome subunit beta 2 / Proteasome beta 3 subunit / Proteasome subunit alpha5 / Proteasome subunit alpha6 / Proteasome beta-type subunits signature. / Peptidase T1A, proteasome beta-subunit / Proteasome beta-type subunit, conserved site ...Proteasome beta subunit, C-terminal / Proteasome beta subunits C terminal / Proteasome subunit beta 4 / Proteasome subunit beta 2 / Proteasome beta 3 subunit / Proteasome subunit alpha5 / Proteasome subunit alpha6 / Proteasome beta-type subunits signature. / Peptidase T1A, proteasome beta-subunit / Proteasome beta-type subunit, conserved site / Proteasome subunit A N-terminal signature / Proteasome alpha-type subunits signature. / Proteasome alpha-subunit, N-terminal domain / Proteasome subunit A N-terminal signature Add an annotation / Proteasome B-type subunit / Proteasome beta-type subunit profile. / : / Proteasome alpha-type subunit / Proteasome alpha-type subunit profile. / Proteasome subunit / Proteasome, subunit alpha/beta / Nucleophile aminohydrolases, N-terminal
Similarity search - Domain/homology
Probable proteasome subunit alpha type-7 / Proteasome subunit alpha type-1 / Proteasome subunit beta type-4 / Proteasome subunit alpha type-3 / Proteasome subunit alpha type-2 / Proteasome subunit beta type-6 / Proteasome subunit beta type-2 / Proteasome subunit beta type-3 / Proteasome subunit beta type-9 / Proteasome subunit beta type-5 ...Probable proteasome subunit alpha type-7 / Proteasome subunit alpha type-1 / Proteasome subunit beta type-4 / Proteasome subunit alpha type-3 / Proteasome subunit alpha type-2 / Proteasome subunit beta type-6 / Proteasome subunit beta type-2 / Proteasome subunit beta type-3 / Proteasome subunit beta type-9 / Proteasome subunit beta type-5 / Proteasome subunit beta type-7 / Proteasome subunit alpha type-5 / Proteasome subunit beta type-1 / Proteasome subunit alpha type-6 / Proteasome subunit alpha type-4
Similarity search - Component
Biological speciesHomo sapiens (human)
Saccharomyces cerevisiae (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.85 Å
AuthorsMaurits, E. / Huber, E.M. / Dekker, P.M. / Wang, X. / Heinemeyer, W. / Florea, B.I. / Groll, M. / Overkleeft, H.S.
Funding support Germany, 1items
OrganizationGrant numberCountry
German Research Foundation (DFG)GR 1861/10-3 Germany
CitationJournal: To Be Published
Title: Structure-based design of peptide epoxyketones selectively targeting the three human immunoproteasome active sites
Authors: Maurits, E. / Huber, E.M. / Dekker, P.M. / Wang, X. / Heinemeyer, W. / Florea, B.I. / Groll, M. / Overkleeft, H.S.
History
DepositionJun 19, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Apr 30, 2025Provider: repository / Type: Initial release
Revision 1.1Sep 10, 2025Group: Database references / Structure summary / Category: audit_author / citation_author / Item: _audit_author.name / _citation_author.name

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Proteasome subunit alpha type-2
B: Proteasome subunit alpha type-3
C: Proteasome subunit alpha type-4
D: Proteasome subunit alpha type-5
E: Proteasome subunit alpha type-6
F: Probable proteasome subunit alpha type-7
G: Proteasome subunit alpha type-1
H: Proteasome subunit beta type-2
I: Proteasome subunit beta type-3
J: Proteasome subunit beta type-4
K: Proteasome subunit beta type-5
L: Proteasome subunit beta type-6
M: Proteasome subunit beta type-7
N: Proteasome subunit beta type-9,Proteasome subunit beta type-1
O: Proteasome subunit alpha type-2
P: Proteasome subunit alpha type-3
Q: Proteasome subunit alpha type-4
R: Proteasome subunit alpha type-5
S: Proteasome subunit alpha type-6
T: Probable proteasome subunit alpha type-7
U: Proteasome subunit alpha type-1
V: Proteasome subunit beta type-2
W: Proteasome subunit beta type-3
X: Proteasome subunit beta type-4
Y: Proteasome subunit beta type-5
Z: Proteasome subunit beta type-6
a: Proteasome subunit beta type-7
b: Proteasome subunit beta type-9,Proteasome subunit beta type-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)731,80648
Polymers730,75628
Non-polymers1,05020
Water2,774154
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration, AU contains one biological assembly
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area120950 Å2
ΔGint-551 kcal/mol
Surface area216130 Å2
MethodPISA
Unit cell
Length a, b, c (Å)135.430, 301.910, 145.530
Angle α, β, γ (deg.)90.00, 112.83, 90.00
Int Tables number4
Space group name H-MP1211

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Components

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Proteasome subunit alpha type- ... , 6 types, 12 molecules AOBPCQDRESGU

#1: Protein Proteasome subunit alpha type-2 / Macropain subunit Y7 / Multicatalytic endopeptidase complex subunit Y7 / Proteasome component Y7 / ...Macropain subunit Y7 / Multicatalytic endopeptidase complex subunit Y7 / Proteasome component Y7 / Proteinase YSCE subunit 7


Mass: 27191.828 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P23639
#2: Protein Proteasome subunit alpha type-3 / Macropain subunit Y13 / Multicatalytic endopeptidase complex subunit Y13 / Proteasome component Y13 ...Macropain subunit Y13 / Multicatalytic endopeptidase complex subunit Y13 / Proteasome component Y13 / Proteinase YSCE subunit 13


Mass: 28748.230 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P23638
#3: Protein Proteasome subunit alpha type-4 / Macropain subunit PRE6 / Multicatalytic endopeptidase complex subunit PRE6 / Proteasome component ...Macropain subunit PRE6 / Multicatalytic endopeptidase complex subunit PRE6 / Proteasome component PRE6 / Proteinase YSCE subunit PRE6


Mass: 28478.111 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P40303
#4: Protein Proteasome subunit alpha type-5 / Macropain subunit PUP2 / Multicatalytic endopeptidase complex subunit PUP2 / Proteasome component ...Macropain subunit PUP2 / Multicatalytic endopeptidase complex subunit PUP2 / Proteasome component PUP2 / Proteinase YSCE subunit PUP2


Mass: 28649.086 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P32379
#5: Protein Proteasome subunit alpha type-6 / Macropain subunit PRE5 / Multicatalytic endopeptidase complex subunit PRE5 / Proteasome component ...Macropain subunit PRE5 / Multicatalytic endopeptidase complex subunit PRE5 / Proteasome component PRE5 / Proteinase YSCE subunit PRE5


Mass: 25634.000 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P40302
#7: Protein Proteasome subunit alpha type-1 / Macropain subunit C7-alpha / Multicatalytic endopeptidase complex C7 / Proteasome component C7- ...Macropain subunit C7-alpha / Multicatalytic endopeptidase complex C7 / Proteasome component C7-alpha / Proteasome component Y8 / Proteinase YSCE subunit 7 / SCL1 suppressor protein


Mass: 28033.830 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P21243

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Protein , 1 types, 2 molecules FT

#6: Protein Probable proteasome subunit alpha type-7 / Macropain subunit C1 / Multicatalytic endopeptidase complex subunit C1 / Proteasome component C1 / ...Macropain subunit C1 / Multicatalytic endopeptidase complex subunit C1 / Proteasome component C1 / Proteinase YSCE subunit 1


Mass: 31575.068 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P21242

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Proteasome subunit beta type- ... , 7 types, 14 molecules HVIWJXKYLZMaNb

#8: Protein Proteasome subunit beta type-2 / Macropain subunit PUP1 / Multicatalytic endopeptidase complex subunit PUP1 / Proteasome component ...Macropain subunit PUP1 / Multicatalytic endopeptidase complex subunit PUP1 / Proteasome component PUP1 / Proteinase YSCE subunit PUP1


Mass: 25114.459 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast)
References: UniProt: P25043, proteasome endopeptidase complex
#9: Protein Proteasome subunit beta type-3 / Macropain subunit PUP3 / Multicatalytic endopeptidase complex subunit PUP3 / Proteasome component PUP3


Mass: 22627.842 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P25451
#10: Protein Proteasome subunit beta type-4 / Macropain subunit C11 / Multicatalytic endopeptidase complex subunit C11 / Proteasome component C11 ...Macropain subunit C11 / Multicatalytic endopeptidase complex subunit C11 / Proteasome component C11 / Proteinase YSCE subunit 11


Mass: 22545.676 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P22141
#11: Protein Proteasome subunit beta type-5 / Macropain subunit PRE2 / Multicatalytic endopeptidase complex subunit PRE2 / Proteasome component ...Macropain subunit PRE2 / Multicatalytic endopeptidase complex subunit PRE2 / Proteasome component PRE2 / Proteinase YSCE subunit PRE2


Mass: 23325.248 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast)
References: UniProt: P30656, proteasome endopeptidase complex
#12: Protein Proteasome subunit beta type-6 / Multicatalytic endopeptidase complex subunit C5 / Proteasome component C5


Mass: 24883.928 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P23724
#13: Protein Proteasome subunit beta type-7 / Macropain subunit PRE4 / Multicatalytic endopeptidase complex subunit PRE4 / Proteasome component ...Macropain subunit PRE4 / Multicatalytic endopeptidase complex subunit PRE4 / Proteasome component PRE4 / Proteinase YSCE subunit PRE4


Mass: 27200.893 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P30657
#14: Protein Proteasome subunit beta type-9,Proteasome subunit beta type-1 / Low molecular mass protein 2 / Macropain chain 7 / Multicatalytic endopeptidase complex chain 7 / ...Low molecular mass protein 2 / Macropain chain 7 / Multicatalytic endopeptidase complex chain 7 / Proteasome chain 7 / Proteasome subunit beta-1i / Really interesting new gene 12 protein / Macropain subunit PRE3 / Multicatalytic endopeptidase complex subunit PRE3 / Proteasome component PRE3 / Proteinase YSCE subunit PRE3


Mass: 21369.938 Da / Num. of mol.: 2 / Mutation: Sequence 1-53 from human beta1i
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: PSMB9, LMP2, PSMB6i, RING12, PRE3, YJL001W, J1407 / Production host: Saccharomyces cerevisiae (brewer's yeast)
References: UniProt: P28065, UniProt: P38624, proteasome endopeptidase complex

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Non-polymers , 5 types, 174 molecules

#15: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: Cl
#16: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 9 / Source method: obtained synthetically / Formula: Mg
#17: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#18: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#19: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 154 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.75 Å3/Da / Density % sol: 67.19 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.8 / Details: 20 mM MgAC2, 13% MPD, 0.1 M MES

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 23, 2021
RadiationMonochromator: LN2 COOLED FIXED-EXIT. SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.85→30 Å / Num. obs: 234648 / % possible obs: 93.8 % / Redundancy: 3 % / Rmerge(I) obs: 0.085 / Net I/σ(I): 9.4
Reflection shellResolution: 2.85→2.95 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.662 / Mean I/σ(I) obs: 2.1 / Num. unique obs: 23855 / % possible all: 97.3

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Processing

Software
NameVersionClassification
REFMAC5.8.0267refinement
XDSdata reduction
XSCALEdata scaling
REFMACphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.85→30 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.937 / SU B: 35.223 / SU ML: 0.277 / Cross valid method: THROUGHOUT / ESU R Free: 0.317 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.22743 11725 5 %RANDOM
Rwork0.18257 ---
obs0.18484 222774 93.87 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 65.325 Å2
Baniso -1Baniso -2Baniso -3
1--0.55 Å2-0 Å2-4.28 Å2
2--1.74 Å20 Å2
3---1.78 Å2
Refinement stepCycle: 1 / Resolution: 2.85→30 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms49255 0 50 154 49459
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0020.01350205
X-RAY DIFFRACTIONr_bond_other_d0.0010.01547717
X-RAY DIFFRACTIONr_angle_refined_deg1.1881.64167921
X-RAY DIFFRACTIONr_angle_other_deg1.061.582109967
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.11356305
X-RAY DIFFRACTIONr_dihedral_angle_2_deg31.41822.8272536
X-RAY DIFFRACTIONr_dihedral_angle_3_deg14.702158727
X-RAY DIFFRACTIONr_dihedral_angle_4_deg14.40715283
X-RAY DIFFRACTIONr_chiral_restr0.0350.26616
X-RAY DIFFRACTIONr_gen_planes_refined0.0020.0257118
X-RAY DIFFRACTIONr_gen_planes_other0.0010.0211284
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it2.6536.36925304
X-RAY DIFFRACTIONr_mcbond_other2.6536.36925303
X-RAY DIFFRACTIONr_mcangle_it3.9469.54731581
X-RAY DIFFRACTIONr_mcangle_other3.9469.54731582
X-RAY DIFFRACTIONr_scbond_it2.4396.66424901
X-RAY DIFFRACTIONr_scbond_other2.4376.66324891
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other3.5859.88336329
X-RAY DIFFRACTIONr_long_range_B_refined5.49272.44851968
X-RAY DIFFRACTIONr_long_range_B_other5.47872.44551954
X-RAY DIFFRACTIONr_rigid_bond_restr0.349397922
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 2.85→2.923 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.344 890 -
Rwork0.297 16897 -
obs--97.41 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.0834-0.01660.01840.0699-0.01240.0346-0.00660.00120.00320.00120.0022-0.006-0.00730.00410.00440.0872-0.00140.01870.004-0.00160.076566.4828-92.171846.2072
20.07460.03120.00510.01580.01550.09420.0045-0.00430.0032-0.00220.00190.00280.00010.0114-0.00640.08550.00010.01760.00530.00370.077759.0669-87.904216.4566
30.06790.030.0190.01560.00080.0711-0.00230.01350.0052-0.0060.00580.0001-0.0056-0.0015-0.00350.08990.00160.01840.00340.00260.076831.8315-87.62451.1575
40.03040.0102-0.01320.0492-0.0170.0303-0.0017-0.00470.0034-0.01010.00210.0103-0.0052-0.0049-0.00040.08660.00240.01570.00280.0010.08292.6389-90.651913.7118
50.01580.00370.0370.01420.01120.0941-0.0092-0.00640.0052-0.00540.00360.0096-0.0042-0.01330.00560.08230.00320.02030.006-0.00270.0851-3.653-95.111645.8533
60.1025-0.0046-0.00610.01040.00690.0661-0.0003-0.00710.00620.0048-0.00040.0102-0.0007-0.00350.00070.0855-0.00060.02110.0052-0.00480.077714.8704-95.691170.0804
70.0434-0.01830.00750.04090.01430.1286-0.00230.00280.00130.012-0.0032-0.0048-0.00120.00630.00550.0872-0.0030.01860.0027-0.00220.076947.3048-93.756871.3386
80.02160.00810.00230.024-0.00340.02310.001-0.0043-0.00020.00430.0004-0.0003-0.00250.0146-0.00140.0876-0.00070.01530.012-0.00070.076467.7436-129.634847.6038
90.0867-0.0162-0.01920.13910.02250.0394-0.00340.00330.003-0.00270.0062-0.0059-0.0020.0038-0.00290.08560.00030.01920.00640.00120.07468.3271-127.386320.9934
100.1344-0.03970.05810.04250.01030.05110.01030.01520.0022-0.0067-0.00540.00420.00220.0068-0.00490.090.0010.01810.0097-0.0010.073444.6227-126.9574-0.6262
110.03980.0585-0.00110.12260.01210.0396-0.00510.0099-0.0009-0.01220.00590.0085-0.00160.0061-0.00080.08850.00220.01520.00740.00140.078510.9321-131.51752.6302
120.04640.01130.0070.04380.02940.05480.0009-0.0016-0.0005-0.003-0.00330.01370.0027-0.00240.00230.08420.00090.01810.00630.00070.0818-4.4634-135.368628.4855
130.0564-0.00510.01390.06340.03170.0319-0.0015-0.0032-0.00090.009500.00540.0003-0.00520.00150.08570.00020.02190.0064-0.00120.07717.4228-137.218660.3216
140.1279-0.07080.01120.09540.03280.04220.0017-0.01460.00360.01120.0082-0.0057-0.00040.0014-0.00990.0877-0.00320.01510.0034-0.00020.076139.6814-134.568570.949
150.0951-0.0209-0.03260.04640.01490.0564-0.00480.0034-0.00360.00470.00050.00610.0135-0.00830.00430.0887-0.00460.01550.00180.00380.07762.6475-207.733437.0063
160.10840.0560.02190.03020.01320.09820.0021-0.0001-0.0013-0.0011-0.00120.00790.005-0.0062-0.00090.0871-0.00070.01720.0006-0.0040.07679.1994-206.78946.8337
170.03470.0132-0.01690.04780.02450.04930.00350.0059-0.0026-0.0173-0.00060.01150.0049-0.0007-0.0030.09220.00290.01320.0029-0.00250.078936.3201-204.6419-8.9497
180.05660.0281-0.00530.0526-0.00120.0347-0.003-0.0014-0.0024-0.0089-0.0051-0.00630.00860.00270.00810.08460.00470.01850.0062-0.00250.075665.7532-203.72053.5447
190.0490.0213-0.02610.0498-0.04440.0413-0.0012-0.0113-0.0105-0.0014-0.0037-0.00910.00390.00530.00490.0850.00590.0160.0036-0.00310.083472.7633-204.664435.7585
200.10790.01490.02660.00260.00630.03610.0066-0.0087-0.01360.001-0.0002-0.00660.0072-0.0045-0.00640.09330.00150.01470.00360.00340.080854.7598-208.269660.0766
210.068-0.01390.00710.0353-0.0480.0688-0.0025-0.00170.00360.0041-0.00030.00350.00140.0010.00280.0895-0.00270.01630.00040.00370.07722.4106-210.600161.5248
220.0267-0.00770.02120.0033-0.00590.0421-0.0052-0.00590.0068-0.004-0.00060.0038-0.0017-0.0160.00570.08470.00060.00940.0166-0.00520.08311.5189-171.050344.7704
230.1080.02530.02490.1255-0.02890.08350.00140-0.0038-0.00850.00280.00660.0026-0.0067-0.00420.08430.00030.01570.0008-0.00190.07890.347-168.780618.1281
240.10110.0255-0.01550.1163-0.0320.01250.011-0.0034-0.0001-0.0122-0.00820.01370.00730.0067-0.00280.08830.00230.01670.0072-0.00280.072623.5053-165.5046-3.714
250.00470.00610.00750.1184-0.02390.02510.00310.0010.001-0.01490.0021-0.00140.00910.005-0.00520.08640.00250.01960.0067-0.00160.075257.3046-161.5236-0.3831
260.01450.0211-0.00350.0597-0.01860.02780.002-0.0053-0.0015-0.00180.0006-0.00910.0030.0065-0.00250.0840.00130.01930.0101-0.00110.078773.2869-162.091625.4329
270.064-0.029-0.00160.0671-0.03380.02390.0001-0.00470.00370.00590.0048-0.0112-0.002-0.0016-0.00490.08760.00140.01660.0053-0.00060.075361.9828-165.158857.4918
280.1261-0.10660.02230.17970.01950.067-0.0054-0.0045-0.00820.01250.00760.0162-0.00250.012-0.00210.0817-0.00640.01920.00880.00060.071830.1177-170.131968.0397
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A1 - 250
2X-RAY DIFFRACTION2B1 - 244
3X-RAY DIFFRACTION3C1 - 240
4X-RAY DIFFRACTION4D1 - 242
5X-RAY DIFFRACTION5E3 - 233
6X-RAY DIFFRACTION6F2 - 244
7X-RAY DIFFRACTION7G2 - 242
8X-RAY DIFFRACTION8H1 - 226
9X-RAY DIFFRACTION9I1 - 204
10X-RAY DIFFRACTION10J1 - 195
11X-RAY DIFFRACTION11K1 - 212
12X-RAY DIFFRACTION12L1 - 222
13X-RAY DIFFRACTION13M1 - 225
14X-RAY DIFFRACTION14N1 - 196
15X-RAY DIFFRACTION15O1 - 250
16X-RAY DIFFRACTION16P1 - 244
17X-RAY DIFFRACTION17Q1 - 240
18X-RAY DIFFRACTION18R1 - 242
19X-RAY DIFFRACTION19S3 - 233
20X-RAY DIFFRACTION20T2 - 244
21X-RAY DIFFRACTION21U2 - 242
22X-RAY DIFFRACTION22V1 - 226
23X-RAY DIFFRACTION23W1 - 204
24X-RAY DIFFRACTION24X1 - 195
25X-RAY DIFFRACTION25Y1 - 212
26X-RAY DIFFRACTION26Z1 - 222
27X-RAY DIFFRACTION27a1 - 228
28X-RAY DIFFRACTION28b1 - 196

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