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- PDB-9f4q: UP1 in complex with Z641239276 -

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Basic information

Entry
Database: PDB / ID: 9f4q
TitleUP1 in complex with Z641239276
ComponentsHeterogeneous nuclear ribonucleoprotein A1, N-terminally processed
KeywordsRNA BINDING PROTEIN / fragment screening / hnRNP A1 / UP1 / RNA/DNA BINDING PROTEIN / UP1-fragment complex
Function / homology
Function and homology information


cellular response to sodium arsenite / SARS-CoV-1-host interactions / import into nucleus / telomeric repeat-containing RNA binding / G-rich strand telomeric DNA binding / pre-mRNA binding / nuclear export / RNA export from nucleus / miRNA binding / FGFR2 alternative splicing ...cellular response to sodium arsenite / SARS-CoV-1-host interactions / import into nucleus / telomeric repeat-containing RNA binding / G-rich strand telomeric DNA binding / pre-mRNA binding / nuclear export / RNA export from nucleus / miRNA binding / FGFR2 alternative splicing / regulation of alternative mRNA splicing, via spliceosome / intracellular non-membrane-bounded organelle / SARS-CoV-1 modulates host translation machinery / regulation of RNA splicing / negative regulation of telomere maintenance via telomerase / Processing of Capped Intron-Containing Pre-mRNA / mRNA transport / localization / cellular response to glucose starvation / positive regulation of telomere maintenance via telomerase / catalytic step 2 spliceosome / molecular condensate scaffold activity / mRNA Splicing - Major Pathway / mRNA 3'-UTR binding / spliceosomal complex / mRNA splicing, via spliceosome / single-stranded DNA binding / amyloid fibril formation / single-stranded RNA binding / ribonucleoprotein complex / protein domain specific binding / DNA binding / RNA binding / extracellular exosome / nucleoplasm / membrane / identical protein binding / nucleus / cytosol / cytoplasm
Similarity search - Function
hnRNP A3, RNA recognition motif 2 / hnRNP A1, RNA recognition motif 1 / Heterogeneous nuclear ribonucleoprotein A1/A2, C-terminal / Heterogeneous nuclear ribonucleoprotein A1, LC domain / RNA recognition motif / RNA recognition motif / Eukaryotic RNA Recognition Motif (RRM) profile. / RNA recognition motif domain / RNA-binding domain superfamily / Nucleotide-binding alpha-beta plait domain superfamily
Similarity search - Domain/homology
: / Heterogeneous nuclear ribonucleoprotein A1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.4 Å
AuthorsDunnett, L. / Prischi, F.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Enhanced identification of small molecules binding to hnRNP A1 via in silico hotspot and cryptic pockets mapping coupled with X-Ray fragment screening
Authors: Dunnett, L. / Prischi, F.
History
DepositionApr 28, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 8, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed
hetero molecules


Theoretical massNumber of molelcules
Total (without water)22,5882
Polymers22,3571
Non-polymers2311
Water3,549197
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: NMR relaxation study
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area0 Å2
ΔGint0 kcal/mol
Surface area9860 Å2
MethodPISA
Unit cell
Length a, b, c (Å)37.930, 43.934, 55.973
Angle α, β, γ (deg.)90.000, 94.070, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

#1: Protein Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed


Mass: 22357.109 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HNRNPA1, HNRPA1 / Plasmid: pETM-14 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P09651
#2: Chemical ChemComp-A1H9S / 5-(2-phenylethylamino)pyrimidine-2,4-diol


Mass: 231.251 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C12H13N3O2 / Feature type: SUBJECT OF INVESTIGATION
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 197 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.07 Å3/Da / Density % sol: 40.66 %
Crystal growTemperature: 289 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: 0.1 M Tris-HCl, pH 8.50, 25% PEG-4000, 8% 2-Methyl-2,4-pentanediol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.91587 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jun 29, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.91587 Å / Relative weight: 1
ReflectionResolution: 1.4→55.83 Å / Num. obs: 33320 / % possible obs: 92 % / Redundancy: 1.6 % / Biso Wilson estimate: 14.03 Å2 / CC1/2: 0.995 / Rmerge(I) obs: 0.043 / Rpim(I) all: 0.043 / Rrim(I) all: 0.061 / Χ2: 0.79 / Net I/σ(I): 9.1 / Num. measured all: 54783
Reflection shellResolution: 1.4→1.42 Å / % possible obs: 65.4 % / Redundancy: 1.2 % / Rmerge(I) obs: 0.19 / Num. measured all: 1418 / Num. unique obs: 1173 / CC1/2: 0.898 / Rpim(I) all: 0.19 / Rrim(I) all: 0.269 / Χ2: 0.79 / Net I/σ(I) obs: 2.4

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
Aimlessdata scaling
xia2data reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.4→55.83 Å / SU ML: 0.1281 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 20.7846
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2009 1692 5.08 %
Rwork0.1819 31617 -
obs0.1828 33309 91.65 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 18.82 Å2
Refinement stepCycle: LAST / Resolution: 1.4→55.83 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1330 0 17 197 1544
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00861402
X-RAY DIFFRACTIONf_angle_d1.08161887
X-RAY DIFFRACTIONf_chiral_restr0.09201
X-RAY DIFFRACTIONf_plane_restr0.0122245
X-RAY DIFFRACTIONf_dihedral_angle_d15.5028536
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.4-1.440.24511030.24372008X-RAY DIFFRACTION70.7
1.44-1.490.24811240.22612384X-RAY DIFFRACTION82.53
1.49-1.540.23881080.20822447X-RAY DIFFRACTION85.57
1.54-1.60.22591160.18682442X-RAY DIFFRACTION84.76
1.6-1.680.23171500.18832725X-RAY DIFFRACTION95.58
1.68-1.760.19411480.19422747X-RAY DIFFRACTION95.92
1.76-1.870.21111660.19062761X-RAY DIFFRACTION96.38
1.87-2.020.20771430.17342771X-RAY DIFFRACTION96.84
2.02-2.220.18291170.17062833X-RAY DIFFRACTION96.91
2.22-2.540.1791790.17882774X-RAY DIFFRACTION97.56
2.54-3.210.21871840.18192810X-RAY DIFFRACTION98.2
3.21-55.830.18441540.1742915X-RAY DIFFRACTION98.43

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