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- PDB-9eu3: GH29A alpha-L-fucosidase -

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Basic information

Entry
Database: PDB / ID: 9eu3
TitleGH29A alpha-L-fucosidase
ComponentsAlpha-L-fucosidase
KeywordsHYDROLASE / GH29A / GH29 / fucosidase / fucose
Function / homology
Function and homology information


alpha-L-fucosidase / alpha-L-fucosidase activity / fucose metabolic process / glycoside catabolic process / lysosome
Similarity search - Function
Alpha-L-fucosidase, metazoa-type / Glycoside hydrolase, family 29 / Alpha-L-fucosidase / Alpha-L-fucosidase / Glycoside hydrolase superfamily
Similarity search - Domain/homology
beta-L-fucopyranose / Alpha-L-fucosidase
Similarity search - Component
Biological speciesTannerella forsythia (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.28 Å
AuthorsYang, Y.Y. / Zeuner, B. / Morth, J.P.
Funding support Denmark, 2items
OrganizationGrant numberCountry
Independent Research Fund Denmark - Technology and Production Sciences1134-00002B Denmark
Other government202108320321
Citation
Journal: Febs J. / Year: 2025
Title: Structural elucidation and characterization of GH29A alpha-l-fucosidases and the effect of pH on their transglycosylation.
Authors: Yang, Y. / Holck, J. / Thorhallsson, A.T. / Hunt, C.J. / Yang, H. / Morth, J.P. / Meyer, A.S. / Zeuner, B.
#1: Journal: Acta Crystallogr.,Sect.D / Year: 2012
Title: Towards automated crystallographic structure refinement with phenix.refine.
Authors: Afonine, P.V. / Grosse-Kunstleve, R.W. / Echols, N. / Headd, J.J. / Moriarty, N.W. / Mustyakimov, M. / Terwilliger, T.C. / Urzhumtsev, A. / Zwart, P.H. / Adams, P.D.
#2: Journal: Acta Crystallogr D Struct Biol / Year: 2019
Title: Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix.
Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty ...Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty / Robert D Oeffner / Billy K Poon / Michael G Prisant / Randy J Read / Jane S Richardson / David C Richardson / Massimo D Sammito / Oleg V Sobolev / Duncan H Stockwell / Thomas C Terwilliger / Alexandre G Urzhumtsev / Lizbeth L Videau / Christopher J Williams / Paul D Adams /
Abstract: Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological ...Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological processes and to develop new therapeutics against diseases. The overall structure-solution workflow is similar for these techniques, but nuances exist because the properties of the reduced experimental data are different. Software tools for structure determination should therefore be tailored for each method. Phenix is a comprehensive software package for macromolecular structure determination that handles data from any of these techniques. Tasks performed with Phenix include data-quality assessment, map improvement, model building, the validation/rebuilding/refinement cycle and deposition. Each tool caters to the type of experimental data. The design of Phenix emphasizes the automation of procedures, where possible, to minimize repetitive and time-consuming manual tasks, while default parameters are chosen to encourage best practice. A graphical user interface provides access to many command-line features of Phenix and streamlines the transition between programs, project tracking and re-running of previous tasks.
History
DepositionMar 27, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 5, 2025Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Alpha-L-fucosidase
B: Alpha-L-fucosidase
C: Alpha-L-fucosidase
D: Alpha-L-fucosidase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)200,22011
Polymers199,3974
Non-polymers8237
Water8,053447
1
A: Alpha-L-fucosidase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,1144
Polymers49,8491
Non-polymers2653
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Alpha-L-fucosidase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,0132
Polymers49,8491
Non-polymers1641
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Alpha-L-fucosidase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,0793
Polymers49,8491
Non-polymers2302
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Alpha-L-fucosidase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,0132
Polymers49,8491
Non-polymers1641
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)146.871, 146.871, 196.703
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number154
Space group name H-MP3221
Space group name HallP322"
Symmetry operation#1: x,y,z
#2: -y,x-y,z+2/3
#3: -x+y,-x,z+1/3
#4: x-y,-y,-z+1/3
#5: -x,-x+y,-z+2/3
#6: y,x,-z
Components on special symmetry positions
IDModelComponents
11C-502-

ZN

Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 25 through 53 or resid 55 through 354 or resid 356 through 446 or resid 501))
d_2ens_1(chain "B" and (resid 25 through 53 or resid 55 through 354 or resid 356 through 446 or resid 501))
d_3ens_1(chain "C" and (resid 25 through 53 or resid 55 through 354 or resid 356 through 446 or resid 501))
d_4ens_1(chain "D" and (resid 25 through 53 or resid 55 through 354 or resid 356 through 446 or resid 501))

NCS domain segments:

Ens-ID: ens_1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11GLYGLYTYRTYRAA25 - 536 - 34
d_12METMETTHRTHRAA55 - 35436 - 335
d_13GLNGLNASNASNAA356 - 446337 - 427
d_14FULFULFULFULAE501
d_21GLYGLYTYRTYRBB25 - 536 - 34
d_22METMETTHRTHRBB55 - 35436 - 335
d_23GLNGLNASNASNBB356 - 446337 - 427
d_24FULFULFULFULBH501
d_31GLYGLYTYRTYRCC25 - 536 - 34
d_32METMETTHRTHRCC55 - 35436 - 335
d_33GLNGLNASNASNCC356 - 446337 - 427
d_34FULFULFULFULCI501
d_41GLYGLYTYRTYRDD25 - 536 - 34
d_42METMETTHRTHRDD55 - 35436 - 335
d_43GLNGLNASNASNDD356 - 446337 - 427
d_44FULFULFULFULDK501

NCS oper:
IDCodeMatrixVector
1given(-0.492535568985, -0.870275533712, -0.00540450796371), (0.870287543609, -0.492502808535, -0.00636985270122), (0.00288179160834, -0.0078408549847, 0.999965107526)-72.6676425552, 127.006876306, 31.7797551317
2given(-0.867515917145, 0.0958234471645, -0.48809220489), (-0.134256509473, 0.899740560651, 0.415261499761), (0.478948142459, 0.425775516549, -0.767674336123)-165.690095456, -48.8003594366, 48.5531607492
3given(0.542855868175, -0.834411516055, -0.0952099168321), (-0.680522505186, -0.370614082679, -0.632087273765), (0.492134764392, 0.407924776883, -0.769025844872)51.2457044293, 8.80932127087, 85.4599989381

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Components

#1: Protein
Alpha-L-fucosidase


Mass: 49849.238 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Tannerella forsythia (bacteria) / Gene: BFO_2737 / Plasmid: pET22b(+) / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: G8UMQ6, alpha-L-fucosidase
#2: Sugar
ChemComp-FUL / beta-L-fucopyranose / beta-L-fucose / 6-deoxy-beta-L-galactopyranose / L-fucose / fucose / 6-DEOXY-BETA-L-GALACTOSE


Type: L-saccharide, beta linking / Mass: 164.156 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C6H12O5 / Feature type: SUBJECT OF INVESTIGATION
IdentifierTypeProgram
LFucpbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
b-L-fucopyranoseCOMMON NAMEGMML 1.0
b-L-FucpIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
FucSNFG CARBOHYDRATE SYMBOLGMML 1.0
#3: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#4: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 447 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.07 Å3/Da / Density % sol: 59.95 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.3 / Details: 8% Ethylene glycol 0.1M HEPES pH 7.3 10% PEG 8000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8731 Å
DetectorType: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Apr 4, 2023
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.8731 Å / Relative weight: 1
ReflectionResolution: 2.28→58.28 Å / Num. obs: 111956 / % possible obs: 97.66 % / Redundancy: 20.7 % / Biso Wilson estimate: 60.17 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.2258 / Rpim(I) all: 0.05089 / Rrim(I) all: 0.2316 / Net I/σ(I): 7.92
Reflection shellResolution: 2.28→2.36 Å / Redundancy: 21.3 % / Rmerge(I) obs: 1 / Mean I/σ(I) obs: 0.28 / Num. unique obs: 11067 / CC1/2: 0.369 / CC star: 0.734 / Rpim(I) all: 1 / Rrim(I) all: 1 / % possible all: 78.11

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
autoPROCdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.28→58.28 Å / SU ML: 0.4358 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 33.6883
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2431 10019 5.13 %
Rwork0.2121 185214 -
obs0.2137 111956 90.2 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 76.46 Å2
Refinement stepCycle: LAST / Resolution: 2.28→58.28 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13612 0 47 447 14106
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.013914077
X-RAY DIFFRACTIONf_angle_d1.641819113
X-RAY DIFFRACTIONf_chiral_restr0.07292015
X-RAY DIFFRACTIONf_plane_restr0.02552462
X-RAY DIFFRACTIONf_dihedral_angle_d9.11561870
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AAX-RAY DIFFRACTIONTorsion NCS0.521754565538
ens_1d_3AAX-RAY DIFFRACTIONTorsion NCS0.757523484097
ens_1d_4AAX-RAY DIFFRACTIONTorsion NCS0.746130652073
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.28-2.310.4176350.4327634X-RAY DIFFRACTION9.34
2.31-2.330.4886580.43261051X-RAY DIFFRACTION15.25
2.33-2.360.43651610.41163484X-RAY DIFFRACTION50.75
2.36-2.390.43441820.40544169X-RAY DIFFRACTION60.57
2.39-2.420.38813490.39485562X-RAY DIFFRACTION82.22
2.42-2.460.41053580.39636449X-RAY DIFFRACTION93.5
2.46-2.490.40333900.38286770X-RAY DIFFRACTION98.3
2.49-2.530.38773700.37546740X-RAY DIFFRACTION99.66
2.53-2.570.39083760.3736812X-RAY DIFFRACTION99.93
2.57-2.610.37673280.35786898X-RAY DIFFRACTION99.93
2.61-2.650.37453820.35336819X-RAY DIFFRACTION99.93
2.66-2.70.34624220.33836792X-RAY DIFFRACTION99.94
2.7-2.760.37834420.32526792X-RAY DIFFRACTION99.89
2.76-2.810.3133830.30326741X-RAY DIFFRACTION99.82
2.81-2.870.3013960.28946852X-RAY DIFFRACTION99.86
2.87-2.940.28843800.28196850X-RAY DIFFRACTION99.99
2.94-3.010.29332920.26586884X-RAY DIFFRACTION99.97
3.01-3.090.30353600.26026867X-RAY DIFFRACTION100
3.09-3.190.28663700.2626913X-RAY DIFFRACTION100
3.19-3.290.27343720.24756745X-RAY DIFFRACTION99.99
3.29-3.410.28693460.22236901X-RAY DIFFRACTION99.97
3.41-3.540.27243880.21666824X-RAY DIFFRACTION99.99
3.54-3.70.23373400.19366853X-RAY DIFFRACTION99.96
3.7-3.90.22413810.18696864X-RAY DIFFRACTION99.97
3.9-4.140.19714120.16456765X-RAY DIFFRACTION100
4.14-4.460.17663700.1446912X-RAY DIFFRACTION99.99
4.46-4.910.15823460.13426872X-RAY DIFFRACTION99.97
4.91-5.620.18143210.1526854X-RAY DIFFRACTION99.99
5.62-7.080.20963620.17936863X-RAY DIFFRACTION99.94
7.08-58.280.1783470.16456682X-RAY DIFFRACTION97.42
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.288442299370.5975007249531.265942943981.798767245820.8210209747432.135597277510.101520092894-0.206823040511-0.09780708973010.0760746393933-0.149572109598-0.1724527277160.1308324389550.030725236440.05776731531980.473838223581-0.0495167133349-0.009110313319090.5353190997990.04194249251360.395654200979-60.47129946862.896088470438.3053256359
22.962343205710.440600105424-0.2625825172843.249720273050.03255473862912.234969537010.3375841700860.09392814682950.307364719251-0.234576529879-0.0261874387326-0.4868928523-0.006036166941510.72545111475-0.1840147167290.694938969674-0.05454448052350.006079767607520.807071538860.05255574962650.717838196398-44.503709584957.270494171834.5864319419
31.97317249254-0.5158754159921.374498836012.87150281930.3715076651373.139415428640.234714658770.239450358541-0.179686704285-0.126213793818-0.0750981377536-0.2802245608080.1485262189520.286266334094-0.2493744769810.617296050473-0.0305141745826-0.002472121979280.5942618317260.01065349123270.523960802056-57.33255991651.683541919827.8393895966
42.52402525022-0.225514942255-0.3717262076614.781103381960.263282090763.92453965456-0.06244501491690.257288954504-0.0370941008701-0.6843151395620.210053521899-0.2487120049770.09384742093630.23497317663-0.04287704912850.555022241094-0.0574678114074-0.0693593021130.710595285060.001863564472350.497387453768-65.889383030957.685605732617.4397649764
52.711072900831.613706058781.927257495821.377543437821.352559523722.47793260486-0.175418865253-0.05463339236420.291493593664-0.0321814017606-0.09691775291530.181281511966-0.183108731216-0.1536814556490.2627216424410.472274703289-0.0101482493609-0.02759685452950.522106577031-0.03522531463220.446450012115-81.019917553664.030856169921.3928590043
62.170402329651.537727853310.2616456294433.548968006711.28118615124.51836402744-0.01330845797250.127251662422-0.0002342217310270.0185314638271-0.08385173017670.1159221157410.0808913083067-0.4003674605950.10922615830.468180932744-0.0374797114125-0.08933243985980.642461164925-0.06490678863010.529363176674-92.142169891956.5373649049.85744285402
70.5622854451140.056404761581-0.3547056114831.13315344459-0.1016837053931.105027194960.1084628928850.2617631305070.1949839068140.01527775565490.0314878874784-0.1776018506430.1388558935350.256932075068-0.120329469080.7000469303760.0895660586009-0.03829047852560.853901154017-0.02033297057390.783856617772-94.776269839842.353808456162.5997620556
81.146676847710.265580826217-0.6768013297171.185381756810.6639985968091.64790278574-0.105064395843-0.3303720345620.0807345010340.161324288290.124344844049-0.02731397258990.03565734043390.302046606292-0.01790299587030.622452626220.0578675417628-0.1063792067140.8469555371030.02920435239630.757423744358-99.265841725243.54977081372.6483917135
94.66886824010.1036947433630.797196002531.84185892303-0.5995428157442.322653264760.01333337121970.2948839236870.74356038191-0.3536516517790.2615792832840.369973660188-0.1516625848570.0202013729203-0.1187016088650.851012962775-0.0897471652422-0.04951523251990.8478402653080.006891026575890.976156480243-100.78230558859.854843979665.8187207297
103.256588182711.46401191063-0.4142558786812.335549402850.3765581169932.13319869297-0.09662624093630.1375889887460.55155488822-0.3708817434910.0377356484362-0.347833824107-0.1310860680270.3770200419090.08330909761590.640717018008-0.0121156700770.01983916821910.86716055042-0.001965638766710.921484064878-90.548503931554.769950218161.6703296926
111.68212889207-0.757802389808-0.9665860267471.211816944080.3767423818951.525810738770.3937215723580.1742466545960.338128201144-0.08580291601140.00373960930437-0.4398147622750.152277718890.388928844874-0.3094712688630.605558005120.000423961238301-0.01238581641040.8503154148590.0499569947340.838547174789-87.993159499946.358750932154.9465008474
123.24539128332-0.239435917965-0.4793876305832.93523386008-0.5208203778533.653247818350.1340912329490.1189573725430.210351365752-0.2866871707260.239807486611-0.1882437641850.160413328109-0.0667628448177-0.1614075664540.795606306050.05895555775140.0007825292856920.925107773379-0.04873586681010.84115664098-90.499052443341.17981955848.5877956362
132.24180703907-0.653055023289-1.124008916350.8924522405690.214877368641.77346782044-0.077934479288-0.0717194115137-0.17669686967-0.000578680652769-0.023378822267-0.284689411330.3804012716840.348327276940.07580826383090.6938572534790.113958206128-0.01895165283210.7557941101870.01154096478830.791900596657-91.86760239727.194382576655.5344796458
140.663145663102-1.11213717219-0.2202896870732.08434660063-0.1738180147322.06118745679-0.160080490498-0.173716494683-0.27267641693-0.239644723806-0.155994661874-0.599363751790.8040432931960.7598165374750.3635096084480.9476248808350.2556877005030.04175949819050.8754350758410.06741673272510.918736394906-77.818582300216.905240558942.0990793818
153.141881586720.519976545401-1.301468489880.593755026929-0.05568253700883.239042527260.134849580122-0.104720552123-0.387514827293-0.129131619336-0.154513164634-0.4500207370370.7060246769330.692267300690.1096877381850.841399244080.3502831868580.07258859080910.7725034856030.0935577975221.05841203424-76.58963503418.92549295641.0189976069
162.01977378541-0.6000928719580.5780857041061.890457362030.1951576581711.01367803566-0.01507235124920.00987884817135-0.55832207030.207881829358-0.1202599078120.2920220360340.393976428353-0.2273652099150.1555289109330.747102360563-0.02519515525520.1732332418240.607776265846-0.04733345476920.659497961112-128.30164277627.60827634418.8529070227
171.899080138380.06959533704040.1244538677692.60532865334-0.1468348671861.4169051397-0.0801336937690.00104882043926-0.7546951039930.646811239396-0.111490348738-0.2665664765820.7101490890840.0004675839251770.2189943825450.9438472028660.1120658438090.1808944201830.5968832854430.04771780045250.902919889707-115.38382075217.79325841425.036047347
182.44970589476-1.86749527013-0.5935353373561.566996879920.791720354470.803042335452-0.387873262271-0.3879066606160.03374270230450.2779735059420.335907407198-0.1779401928710.1286951172240.05495908827170.05227094171510.7059056387560.110539091060.1063606799420.5903266558670.005097418570990.686896319816-108.01146309635.631930073221.1450762134
192.53687511185-0.542875805609-0.469389256521.065661106160.1484133625781.88005246731-0.283223798982-0.135359750228-0.0732055381109-0.144078291555-0.0177567526966-0.165007425270.316493330280.3802430421440.311997840790.7988879033030.1638474989810.1347251143190.6340311097060.008103110087310.807666379852-89.325991972220.132538602420.1422362957
203.57022002881-0.6866855237540.2616791095563.34809112539-0.3685844623392.77004602145-0.01299773484620.177932124831-0.4864170117120.099800018227-0.0978550274169-0.07089287309580.2896082635420.2092659068630.005161157795420.7147173972980.1711146095770.1617912810750.658288746534-0.00482561702490.854859054305-85.366908240218.609547206420.793066229
211.27384556250.333566548195-0.4637419691083.13831706751-0.8310360957611.075551146660.02930978594320.139863804902-0.350803070911-0.384425847821-0.232356278528-0.9094683447750.2945423690610.356628845180.213435191510.7397532801140.05393919898910.148797192750.684189690440.04704428585810.864340296412-35.37160609851.3195756932853.3458462625
222.141164533270.157173597838-0.3180750694983.424235257860.1129864155632.277993120720.0380917258985-0.0303003592378-0.2324871722610.0896720554264-0.222590781968-1.213600195850.3188752127430.2973468283910.2480816166990.641936444032-0.00306197593660.09083887630520.7248500183520.1551281589220.971656647165-27.395202057913.227360423158.2523017255
230.499658451857-0.1531425436870.5172085281863.225470164550.01100810466461.57305744919-0.1032083125850.220433985747-0.176400496048-0.1417596282720.0415901995761-0.1124727856150.325522913025-0.1047881724670.05256684714240.575661449741-0.03406118295080.08523065483150.606568905510.03173615245660.541745765412-47.769649996321.688005985555.46118903
241.91812784660.699998511411-0.189120993473.970066161310.6453064502623.04294396211-0.169414518115-0.04287401653780.07497141921640.00240820833710.00680976874497-0.000249288369444-0.2830803135540.01889437848350.1301112205060.633525784596-0.0446114675758-0.03067713500170.6311688566620.1295452494310.543162928412-48.171037719343.916038262455.1935820359
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 25 through 164 )AA25 - 1641 - 140
22chain 'A' and (resid 165 through 195 )AA165 - 195141 - 171
33chain 'A' and (resid 196 through 266 )AA196 - 266172 - 242
44chain 'A' and (resid 267 through 287 )AA267 - 287243 - 263
55chain 'A' and (resid 288 through 411 )AA288 - 411264 - 387
66chain 'A' and (resid 412 through 446 )AA412 - 446388 - 422
77chain 'B' and (resid 25 through 69 )BC25 - 691 - 45
88chain 'B' and (resid 70 through 164 )BC70 - 16446 - 140
99chain 'B' and (resid 165 through 195 )BC165 - 195141 - 171
1010chain 'B' and (resid 196 through 237 )BC196 - 237172 - 213
1111chain 'B' and (resid 238 through 266 )BC238 - 266214 - 242
1212chain 'B' and (resid 267 through 287 )BC267 - 287243 - 263
1313chain 'B' and (resid 288 through 382 )BC288 - 382264 - 358
1414chain 'B' and (resid 383 through 411 )BC383 - 411359 - 387
1515chain 'B' and (resid 412 through 446 )BC412 - 446388 - 422
1616chain 'C' and (resid 25 through 237 )CE25 - 2371 - 213
1717chain 'C' and (resid 238 through 287 )CE238 - 287214 - 263
1818chain 'C' and (resid 288 through 354 )CE288 - 354264 - 330
1919chain 'C' and (resid 355 through 412 )CE355 - 412331 - 388
2020chain 'C' and (resid 413 through 446 )CE413 - 446389 - 422
2121chain 'D' and (resid 25 through 195 )DG25 - 1951 - 171
2222chain 'D' and (resid 196 through 287 )DG196 - 287172 - 263
2323chain 'D' and (resid 288 through 382 )DG288 - 382264 - 358
2424chain 'D' and (resid 383 through 446 )DG383 - 446359 - 422

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