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- PDB-9eu2: GH29A alpha-L-fucosidase -

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Basic information

Entry
Database: PDB / ID: 9eu2
TitleGH29A alpha-L-fucosidase
ComponentsAlpha-L-fucosidase
KeywordsHYDROLASE / GH29A / GH29 / fucosidase
Function / homology
Function and homology information


alpha-L-fucosidase / alpha-L-fucosidase activity / fucose metabolic process / glycoside catabolic process / lysosome
Similarity search - Function
Alpha-L-fucosidase, metazoa-type / Glycoside hydrolase, family 29 / Alpha-L-fucosidase / Alpha-L-fucosidase / Glycoside hydrolase superfamily
Similarity search - Domain/homology
Biological speciesTannerella forsythia (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.84 Å
AuthorsYang, Y.Y. / Zeuner, B. / Morth, J.P.
Funding support Denmark, 2items
OrganizationGrant numberCountry
Independent Research Fund Denmark - Technology and Production Sciences1134-00002B Denmark
Other government202108320321
CitationJournal: Febs J. / Year: 2025
Title: Structural elucidation and characterization of GH29A alpha-l-fucosidases and the effect of pH on their transglycosylation.
Authors: Yang, Y. / Holck, J. / Thorhallsson, A.T. / Hunt, C.J. / Yang, H. / Morth, J.P. / Meyer, A.S. / Zeuner, B.
History
DepositionMar 27, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 5, 2025Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Alpha-L-fucosidase
B: Alpha-L-fucosidase
C: Alpha-L-fucosidase
D: Alpha-L-fucosidase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)200,29919
Polymers199,3974
Non-polymers90215
Water32,5351806
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)147.912, 147.912, 197.086
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number154
Space group name H-MP3221
Space group name HallP322"
Symmetry operation#1: x,y,z
#2: -y,x-y,z+2/3
#3: -x+y,-x,z+1/3
#4: x-y,-y,-z+1/3
#5: -x,-x+y,-z+2/3
#6: y,x,-z
Components on special symmetry positions
IDModelComponents
11A-501-

ZN

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Components

#1: Protein
Alpha-L-fucosidase


Mass: 49849.238 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Tannerella forsythia (bacteria) / Gene: BFO_2737 / Plasmid: pET22b(+) / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: G8UMQ6, alpha-L-fucosidase
#2: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Zn
#3: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 11 / Source method: obtained synthetically / Formula: C2H6O2
#4: Chemical ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Na
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1806 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.12 Å3/Da / Density % sol: 60.59 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.3 / Details: 20% Ethylene glycol 0.1M HEPES pH 7.3 10% PEG 8000

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Data collection

DiffractionMean temperature: 100 K / Ambient temp details: 293 / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8731 Å
DetectorType: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Apr 4, 2023
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.8731 Å / Relative weight: 1
ReflectionResolution: 1.84→47.02 Å / Num. obs: 215060 / % possible obs: 99.92 % / Redundancy: 20.7 % / Biso Wilson estimate: 31.79 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.1315 / Rpim(I) all: 0.02981 / Rrim(I) all: 0.1349 / Net I/σ(I): 18
Reflection shellResolution: 1.84→1.906 Å / Redundancy: 20.7 % / Rmerge(I) obs: 1 / Mean I/σ(I) obs: 1.47 / Num. unique obs: 21308 / CC1/2: 0.717 / CC star: 0.914 / Rpim(I) all: 0.5029 / Rrim(I) all: 1 / % possible all: 99.98

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
autoPROCdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.84→47.02 Å / SU ML: 0.2261 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 23.0396
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.188 3908 0.93 %
Rwork0.1623 414262 -
obs0.1625 215060 99.92 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 35.69 Å2
Refinement stepCycle: LAST / Resolution: 1.84→47.02 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13614 0 48 1806 15468
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.006314075
X-RAY DIFFRACTIONf_angle_d0.951719083
X-RAY DIFFRACTIONf_chiral_restr0.05261997
X-RAY DIFFRACTIONf_plane_restr0.01292461
X-RAY DIFFRACTIONf_dihedral_angle_d6.4421880
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.84-1.860.45121310.338714808X-RAY DIFFRACTION99.91
1.86-1.890.34041380.313714715X-RAY DIFFRACTION99.95
1.89-1.910.29221420.29314893X-RAY DIFFRACTION99.97
1.91-1.940.26571400.268814727X-RAY DIFFRACTION99.95
1.94-1.960.26351440.252914875X-RAY DIFFRACTION99.99
1.96-1.990.27031400.235514779X-RAY DIFFRACTION99.97
1.99-2.030.21371360.21914827X-RAY DIFFRACTION99.97
2.03-2.060.2411380.209414806X-RAY DIFFRACTION99.96
2.06-2.090.2311420.20214730X-RAY DIFFRACTION100
2.09-2.130.22091340.191814778X-RAY DIFFRACTION99.99
2.13-2.170.20961440.183214874X-RAY DIFFRACTION99.97
2.17-2.220.23131420.175314797X-RAY DIFFRACTION99.98
2.22-2.270.19931400.181614808X-RAY DIFFRACTION99.96
2.27-2.320.19211380.165114813X-RAY DIFFRACTION99.99
2.32-2.380.19721380.152514771X-RAY DIFFRACTION99.99
2.38-2.440.18491400.146214817X-RAY DIFFRACTION99.97
2.44-2.510.1871440.157814824X-RAY DIFFRACTION99.96
2.51-2.590.18781400.155714859X-RAY DIFFRACTION100
2.59-2.690.19451420.151514727X-RAY DIFFRACTION99.99
2.69-2.790.18061340.143214827X-RAY DIFFRACTION99.93
2.79-2.920.17631420.171214759X-RAY DIFFRACTION99.96
2.92-3.070.18641500.161514811X-RAY DIFFRACTION99.99
3.07-3.270.20861340.155914820X-RAY DIFFRACTION99.98
3.27-3.520.1851420.153314795X-RAY DIFFRACTION100
3.52-3.870.16931340.149114808X-RAY DIFFRACTION99.98
3.87-4.430.14321440.128514796X-RAY DIFFRACTION99.99
4.43-5.580.15951420.121314805X-RAY DIFFRACTION100
5.58-47.020.16941330.166414613X-RAY DIFFRACTION98.57
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
10.714823836427-0.166673759815-0.1304706041111.904294920040.3704959688180.909151671069-0.006626888134420.0555265353918-0.02357045216540.0343032218387-0.1096728993580.3878738039780.0295314736608-0.203404106098-0.001194274106480.2438878694920.017815248388-0.002122289339750.296701577925-0.0599777840460.30173868430333.75627908153.8877739373213.5526986289
20.04640841786290.0625714042155-0.03997803945270.0987725715949-0.04661113458380.08499231876450.07505492828760.155308570590.227864359088-0.333376135061-0.1178791199960.225643358918-0.261307001157-0.238498660423-0.0001436573487470.3860992018260.1100878709-0.09106905529480.367468003733-0.03770325205770.38282041713935.230156178920.92833522564.69510018713
30.1082230436010.260637544608-0.07348286631590.695514470753-0.2062618452830.3502049351810.0109654274326-0.0254662026330.0628264662342-0.034787522453-0.01164117527760.00508436224931-0.058518886396-0.0508718644954-1.14606234087E-50.3108772467850.0556635905145-0.03743119616910.253161108552-0.03811859325540.28072627876448.875024238521.524272907112.17042379
40.1322366424140.1764866295940.0533522428310.2615943225-0.07623133469450.3006438789880.01018042417440.1113840644420.171916539439-0.223431598529-0.0392652718789-0.162096828824-0.1900457426170.160843426412-0.0001453265202480.4249319769360.0837742317003-0.04380142601020.308891271333-0.04671681311360.42181549040950.522570028843.796669199412.5597029978
50.127048714248-0.09040266344730.0185908219730.539041199573-0.01402912240290.5365475600790.001984263295650.08000897267460.134747287812-0.10821418625-0.02841072620520.0746187252825-0.0943624167367-0.0644593651894.44366703633E-50.4273300442850.0770038853572-0.02695528671670.319627905396-0.03675735656040.40699011477547.559728687243.823336595512.0226674241
60.28381659494-0.266824669581-0.2878796133010.4860945398660.08643003364650.4292451270760.02195140457950.04352477978320.0495807503004-0.128632134129-0.056490955440.40853940151-0.0995274561394-0.08364084862556.72733143533E-50.3317631540.00331854782579-0.05754482106940.325887122886-0.0261300219290.42804172182637.4663721773-8.96023453098-42.8240685805
70.2160171607560.1677313155060.08625931633090.25784769182-0.1278430222170.296007374195-0.03120218554180.02774882980690.186700932712-0.41209550790.04654837968210.141967531197-0.1899742860830.02148532495662.72329848189E-50.4056755126140.00061730546681-0.05036667903020.306470496479-0.0007020082202410.34114685544646.52258343812.74468689005-47.2026855861
80.8954410641720.3964827614480.1359895374421.876658367910.2740220967780.660300895262-0.007657119501380.008935006532350.137294119105-0.159438799502-0.1358209856110.719186796476-0.115926829966-0.243798589776-0.0003521697103830.3274000805240.0310240369899-0.09185114844440.35251329504-0.0556584713380.54121128090830.3029634551-3.81887390807-43.3002196455
90.0663034654333-0.0283955306052-0.004004190675290.0857108019973-0.06703175395480.1062180560740.0952338442687-0.139932056688-0.05436465360250.379106291369-0.1275657612890.4824016636150.223035039503-0.103409494207-0.0001159969512420.390203104437-0.05460112516120.04965339239910.368154278217-0.0524149250160.44318378479735.0681475167-21.1425272132-34.9201069976
100.0316964930434-0.04554324776210.05536141363090.5504263062910.1459474896510.305259940128-0.008038425872290.07691703139740.0509121889655-0.019448704940.00760326475636-0.0301345563894-0.08828444237330.0590543858382-5.55400281802E-60.318078587958-0.0251096805318-0.004210770471940.286370782138-0.02343665907970.30206556257251.0160391723-15.9066440788-42.4579238262
110.156063849647-0.102506202149-0.01189413045630.1466565057410.03420466448820.0213413366627-0.07296703040230.0985166208319-0.147242203894-0.1191729854130.03568012300640.2137556737060.0426600147054-0.07659894634121.08962491131E-50.302947614785-0.028299228548-0.01544989995620.294428036598-0.06546882800940.3180540493442.8897542842-35.6381017367-43.3472529714
120.19461632888-0.236926201793-0.1086131452140.3179153283140.007032081147790.31640183108-0.09052146901870.00945340962013-0.002351344905290.02809788348370.00426106281088-0.0204488006190.07963536813480.0788217321315-1.77565837847E-50.334083700958-0.03947312782880.03236958950510.296941950424-0.06667901007270.32691432000250.4481874723-43.8584729434-43.3347688845
130.126381857943-0.0202902796788-0.04299583130650.5764385414890.0006365924376660.459652412236-0.0788607497878-0.0360576304264-0.1040160951920.005888605693830.01187973724830.04153573531760.0962108077509-0.04965688666828.27409248831E-50.312528969625-0.03841603529010.02463588697230.315886980518-0.05703062029510.32482635643848.0917070954-44.0792080377-42.8073568989
140.215022567742-0.06112463608040.1580583193850.1800989334690.07864123042010.2265209173160.07888034915760.305181804787-0.0200009717445-0.1423843438540.10133696027-0.5140224855390.3723493533690.185441358104-9.86599331982E-50.4066512383820.09206409547750.006174928012130.387814972905-0.05566823650040.45009600043274.9243807898-39.0317482458-4.71698252288
150.355158436493-0.01465395986490.09630013470890.9242177865350.430089381810.743109594563-0.02227212298340.0336600522117-0.005825565144810.0346478490411-0.00445893972143-0.05006245026420.03116145762880.0319760893-9.72780237796E-80.2655545686660.0171837789252-0.003293944476570.223927969139-0.002686507952610.1953310475262.9660251804-23.7398313233-1.26864356728
160.381334262230.136176055844-0.02603321664890.7954150384890.06275911208730.886167729616-0.04558470704480.0025842815198-0.0694851680761-0.00386157845831-0.01283661635640.1699758625540.171195206542-0.152923616093-1.22421918877E-50.338740281424-0.02818417808010.01027162120420.289953667697-0.03918085919150.30656729344450.9578136229-51.5525041692-22.2389835881
170.448319920328-0.533576420136-0.1709123344130.855810068421-0.03139748730980.4633199810240.03847869454090.05471812160410.0192203917163-0.08378630623920.02208439440830.0231157946486-0.125425229944-0.01848567070128.52401017269E-60.403789196971-0.01537709811930.01421222177590.269049163420.01449476141980.2827256409862.974643260323.5894774887-29.9871527065
180.2050315726960.208438421789-0.01949705540350.2190390890310.07139788090050.288372361319-0.01318407864510.0856168991266-0.0201175177237-0.240080532819-0.02913925690170.097545049419-0.0802824799452-0.0983246191776-3.75405565607E-50.3971825786938.89610714058E-5-0.01644595715550.2580069849290.02004573103420.28147895609954.950465061617.9515198407-41.2237827076
190.633858121170.3776520959960.04702293921710.4928751798030.2355375878110.8206543734420.01668432925130.1191853877110.0468720851221-0.248388274412-0.0209290091325-0.115516312908-0.1357339002480.060401653413-1.5925566755E-50.404367342683-0.02901782881070.0235373957480.2746259133870.03040908849080.29885069927765.048186065120.2089468591-39.2417744029
200.6757918668530.386211918458-0.01701009576710.7760567184330.3016028023270.9270027403120.0518039206799-0.102441885233-0.05237736983420.07057967050070.0485306661562-0.262108975136-0.02828130613820.1780486734685.13058427361E-50.350456113377-0.03190506175740.01263014402120.3070616138430.01373610499020.35166428884974.499258413319.9251641964-28.2205849514
210.115192984097-0.0805721277565-0.08563394363820.110488571426-0.002232050257980.1102423162090.0236321499774-0.247880015459-0.01290388411580.324581668644-0.00726504731124-0.118018362841-0.09681820899840.02492701993648.97731941162E-50.456891186039-0.02537344134490.001100323832420.3506091786640.006687056866180.33926298996864.60055723528.2550460734-15.7165150076
220.232389784276-0.364371527495-0.07129127712740.4288160344660.07626275739230.518451701588-0.01563733159610.0392707451770.049614016097-0.0688079472765-0.01180952571630.174010075051-0.154703949455-0.135576031982-9.35113715113E-50.4148822410930.0252905159810.007869847956830.2704694834310.0004687170317620.35968512043751.738617124533.8516888236-22.6527961188
230.131298703475-0.156242588134-0.0304755918770.1711846701390.06212030139260.269034831563-0.0691643321918-0.008270485104440.1179054484250.1169179057460.07530693093260.109350124869-0.369759254764-0.205095767622-0.0001244941495350.6261027791250.0888866414034-0.007494468096710.285640273017-0.01441622534020.40748794226449.830491046251.2311724423-9.16473575117
240.1004936716510.08427267447180.1252713789090.35503227702-0.05644458961890.580377645872-0.09786579685330.008721089980230.113335770824-0.01776323263750.1261532882010.0315725062656-0.480585677793-0.0781556025975-5.51058436502E-50.6709435439030.0773514041566-0.0117527262790.356981446465-0.01806016153770.46948851460452.103111375751.3818136175-8.09642377715
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 25 through 266 )AA25 - 2661 - 242
22chain 'A' and (resid 267 through 287 )AA267 - 287243 - 263
33chain 'A' and (resid 288 through 382 )AA288 - 382264 - 358
44chain 'A' and (resid 383 through 412 )AA383 - 412359 - 388
55chain 'A' and (resid 413 through 446 )AA413 - 446389 - 422
66chain 'B' and (resid 26 through 69 )BB26 - 691 - 44
77chain 'B' and (resid 70 through 97 )BB70 - 9745 - 72
88chain 'B' and (resid 98 through 266 )BB98 - 26673 - 241
99chain 'B' and (resid 267 through 287 )BB267 - 287242 - 262
1010chain 'B' and (resid 288 through 354 )BB288 - 354263 - 329
1111chain 'B' and (resid 355 through 382 )BB355 - 382330 - 357
1212chain 'B' and (resid 383 through 412 )BB383 - 412358 - 387
1313chain 'B' and (resid 413 through 445 )BB413 - 445388 - 420
1414chain 'C' and (resid 21 through 42 )CC21 - 421 - 22
1515chain 'C' and (resid 43 through 382 )CC43 - 38223 - 362
1616chain 'C' and (resid 383 through 445 )CC383 - 445363 - 425
1717chain 'D' and (resid 26 through 69 )DD26 - 691 - 44
1818chain 'D' and (resid 70 through 97 )DD70 - 9745 - 72
1919chain 'D' and (resid 98 through 164 )DD98 - 16473 - 139
2020chain 'D' and (resid 165 through 266 )DD165 - 266140 - 241
2121chain 'D' and (resid 267 through 287 )DD267 - 287242 - 262
2222chain 'D' and (resid 288 through 382 )DD288 - 382263 - 357
2323chain 'D' and (resid 383 through 411 )DD383 - 411358 - 386
2424chain 'D' and (resid 412 through 446 )DD412 - 446387 - 421

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