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- PDB-9enn: L-amino acid oxidase 4 (HcLAAO4) from the fungus Hebeloma cylindr... -

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Basic information

Entry
Database: PDB / ID: 9enn
TitleL-amino acid oxidase 4 (HcLAAO4) from the fungus Hebeloma cylindrosporum in complex with N-epsilon-acetyl-L-lysine
ComponentsL-amino acid oxidase 4
KeywordsOXIDOREDUCTASE / L-AMINO ACID OXIDASE / FAD / FLAVOPROTEIN
Function / homology
Function and homology information


L-arginine oxidase / L-glutamate oxidase / Oxidoreductases; Acting on the CH-NH2 group of donors; With oxygen as acceptor / L-lysine oxidase / L-amino-acid oxidase / L-amino-acid oxidase activity / amino acid catabolic process / extracellular region
Similarity search - Function
: / Amine oxidase / Flavin containing amine oxidoreductase / FAD/NAD(P)-binding domain superfamily
Similarity search - Domain/homology
N(6)-ACETYLLYSINE / DIHYDROFLAVINE-ADENINE DINUCLEOTIDE / S-1,2-PROPANEDIOL / R-1,2-PROPANEDIOL / L-amino-acid oxidase 4
Similarity search - Component
Biological speciesHebeloma cylindrosporum (fungus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 1.8 Å
AuthorsGilzer, D. / Koopmeiners, S. / Fischer von Mollard, G. / Niemann, H.H.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: Febs Lett. / Year: 2024
Title: Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum.
Authors: Koopmeiners, S. / Gilzer, D. / Widmann, C. / Berelsmann, N. / Spross, J. / Niemann, H.H. / Fischer von Mollard, G.
History
DepositionMar 13, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 14, 2024Provider: repository / Type: Initial release
Revision 1.1Aug 28, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.2Oct 9, 2024Group: Database references / Structure summary / Category: citation / citation_author / pdbx_entry_details
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID / _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: L-amino acid oxidase 4
B: L-amino acid oxidase 4
C: L-amino acid oxidase 4
D: L-amino acid oxidase 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)258,44675
Polymers248,7914
Non-polymers9,65671
Water27,0591502
1
A: L-amino acid oxidase 4
B: L-amino acid oxidase 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)128,97335
Polymers124,3952
Non-polymers4,57833
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: L-amino acid oxidase 4
D: L-amino acid oxidase 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)129,47340
Polymers124,3952
Non-polymers5,07838
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)84.880, 132.120, 107.320
Angle α, β, γ (deg.)90.000, 95.928, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 65 through 83 or resid 85...
d_2ens_1(chain "B" and (resid 65 through 83 or resid 85...
d_3ens_1(chain "C" and (resid 65 through 83 or resid 85...
d_4ens_1(chain "D" and (resid 65 through 83 or resid 85...

NCS domain segments:

Ens-ID: ens_1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11GLUGLULEULEUAA65 - 8312 - 30
d_12SERSERASPASPAA85 - 13032 - 77
d_13LYSLYSGLYGLYAA132 - 14479 - 91
d_14LEULEUVALVALAA146 - 20593 - 152
d_15ASPASPARGARGAA207 - 240154 - 187
d_16TYRTYRPROPROAA242 - 249189 - 196
d_17PROPROPROPROAA251198
d_18PHEPHESERSERAA253 - 257200 - 204
d_19HISHISASPASPAA259 - 272206 - 219
d_110SERSERLEULEUAA274 - 287221 - 234
d_111ALAALALYSLYSAA289 - 322236 - 269
d_112ALAALAALAALAAA327 - 332274 - 279
d_113VALVALGLYGLYAA334 - 355281 - 302
d_114LYSLYSLYSLYSAA357304
d_115LYSLYSALAALAAA359 - 475306 - 422
d_116TYRTYRLEULEUAA477 - 486424 - 433
d_117ASNASNTHRTHRAA488 - 495435 - 442
d_118TYRTYRVALVALAA497 - 505444 - 452
d_119VALVALASNASNAA507 - 513454 - 460
d_120ASNASNPHEPHEAA515 - 530462 - 477
d_121ASPASPLEULEUAA532 - 537479 - 484
d_122ARGARGGLUGLUAA539 - 598486 - 545
d_124ALYALYALYALYAF702
d_21GLUGLULEULEUBB65 - 8312 - 30
d_22SERSERASPASPBB85 - 13032 - 77
d_23LYSLYSGLYGLYBB132 - 14479 - 91
d_24LEULEUVALVALBB146 - 20593 - 152
d_25ASPASPARGARGBB207 - 240154 - 187
d_26TYRTYRPROPROBB242 - 249189 - 196
d_27PROPROPROPROBB251198
d_28PHEPHESERSERBB253 - 257200 - 204
d_29HISHISASPASPBB259 - 272206 - 219
d_210SERSERLEULEUBB274 - 287221 - 234
d_211ALAALALYSLYSBB289 - 322236 - 269
d_212ALAALAALAALABB327 - 332274 - 279
d_213VALVALGLYGLYBB334 - 355281 - 302
d_214LYSLYSLYSLYSBB357304
d_215LYSLYSALAALABB359 - 475306 - 422
d_216TYRTYRLEULEUBB477 - 486424 - 433
d_217ASNASNTHRTHRBB488 - 495435 - 442
d_218TYRTYRVALVALBB497 - 505444 - 452
d_219VALVALASNASNBB507 - 513454 - 460
d_220ASNASNPHEPHEBB515 - 530462 - 477
d_221ASPASPLEULEUBB532 - 537479 - 484
d_222ARGARGGLUGLUBB539 - 598486 - 545
d_224ALYALYALYALYBV702
d_31GLUGLULEULEUCC65 - 8312 - 30
d_32SERSERASPASPCC85 - 13032 - 77
d_33LYSLYSGLYGLYCC132 - 14479 - 91
d_34LEULEUVALVALCC146 - 20593 - 152
d_35ASPASPARGARGCC207 - 240154 - 187
d_36TYRTYRPROPROCC242 - 249189 - 196
d_37PROPROPROPROCC251198
d_38PHEPHESERSERCC253 - 257200 - 204
d_39HISHISASPASPCC259 - 272206 - 219
d_310SERSERLEULEUCC274 - 287221 - 234
d_311ALAALAVALVALCC289 - 295236 - 242
d_312GLUGLUALAALACC299 - 332246 - 279
d_313VALVALGLYGLYCC334 - 355281 - 302
d_314LYSLYSLYSLYSCC357304
d_315LYSLYSALAALACC359 - 475306 - 422
d_316TYRTYRLEULEUCC477 - 486424 - 433
d_317ASNASNTHRTHRCC488 - 495435 - 442
d_318TYRTYRVALVALCC497 - 505444 - 452
d_319VALVALASNASNCC507 - 513454 - 460
d_320ASNASNPHEPHECC515 - 530462 - 477
d_321ASPASPLEULEUCC532 - 537479 - 484
d_322ARGARGGLUGLUCC539 - 598486 - 545
d_324ALYALYALYALYCMA702
d_41GLUGLULEULEUDD65 - 8312 - 30
d_42SERSERASPASPDD85 - 13032 - 77
d_43LYSLYSGLYGLYDD132 - 14479 - 91
d_44LEULEUVALVALDD146 - 20593 - 152
d_45ASPASPARGARGDD207 - 240154 - 187
d_46TYRTYRPROPRODD242 - 249189 - 196
d_47PROPROPROPRODD251198
d_48PHEPHESERSERDD253 - 257200 - 204
d_49HISHISASPASPDD259 - 272206 - 219
d_410SERSERLEULEUDD274 - 287221 - 234
d_411ALAALAVALVALDD289 - 295236 - 242
d_412GLUGLULYSLYSDD299 - 322246 - 269
d_413ALAALAALAALADD327 - 332274 - 279
d_414VALVALGLYGLYDD334 - 355281 - 302
d_415LYSLYSLYSLYSDD357304
d_416LYSLYSALAALADD359 - 475306 - 422
d_417TYRTYRLEULEUDD477 - 486424 - 433
d_418ASNASNTHRTHRDD488 - 495435 - 442
d_419TYRTYRVALVALDD497 - 505444 - 452
d_420VALVALASNASNDD507 - 513454 - 460
d_421ASNASNPHEPHEDD515 - 530462 - 477
d_422ASPASPLEULEUDD532 - 537479 - 484
d_423ARGARGGLUGLUDD539 - 598486 - 545
d_425ALYALYALYALYDGB702

NCS oper:
IDCodeMatrixVector
1given(-0.99994190034, 0.00501202811349, -0.00954334944518), (-0.00570721864788, -0.99722184959, 0.0742698482284), (-0.0091445940177, 0.0743199991574, 0.99719251608)40.5484679132, -10.0003219171, 0.571997948131
2given(0.419147110549, -0.907914342715, -0.00269184148272), (0.907911637271, 0.419153563831, -0.00259785227973), (0.00348692229578, -0.00135507193116, 0.999993002552)-1.41409418687, -21.1862597049, 51.8759641
3given(-0.415659086114, 0.905974319189, -0.0802375043299), (-0.909440239853, -0.415173820836, 0.02343392048), (-0.0120819810942, 0.0827117371538, 0.996500273091)24.8933310818, 11.1573373196, 52.6470099339

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Components

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Protein , 1 types, 4 molecules ABCD

#1: Protein
L-amino acid oxidase 4


Mass: 62197.676 Da / Num. of mol.: 4 / Mutation: K474A, K475A
Source method: isolated from a genetically manipulated source
Details: residues 54 - 615 obtained by limited proteolysis; surface entropy reduction mutations K474A and K475A
Source: (gene. exp.) Hebeloma cylindrosporum (fungus) / Plasmid: pET28b / Production host: Escherichia coli (E. coli) / Strain (production host): Arctic Express / References: UniProt: S4S6Z0, L-amino-acid oxidase

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Non-polymers , 6 types, 1573 molecules

#2: Chemical
ChemComp-FDA / DIHYDROFLAVINE-ADENINE DINUCLEOTIDE


Mass: 787.566 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C27H35N9O15P2 / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical
ChemComp-ALY / N(6)-ACETYLLYSINE


Type: L-peptide linking / Mass: 188.224 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C8H16N2O3 / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical...
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 48 / Source method: obtained synthetically / Formula: SO4
#5: Chemical
ChemComp-PGR / R-1,2-PROPANEDIOL


Mass: 76.094 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C3H8O2
#6: Chemical
ChemComp-PGO / S-1,2-PROPANEDIOL


Mass: 76.094 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C3H8O2
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1502 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.41 Å3/Da / Density % sol: 48.87 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7
Details: protein: 10 mg/ml; reservoir solution: 2.15 M (NH4)2SO4, 0.1 M sodium citrate, pH 7.0; drop with 200 nl protein + 200 nl reservoir

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID30B / Wavelength: 0.97625 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Apr 24, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97625 Å / Relative weight: 1
ReflectionResolution: 1.8→50 Å / Num. obs: 214609 / % possible obs: 98.8 % / Redundancy: 6.24 % / Biso Wilson estimate: 31.82 Å2 / CC1/2: 0.999 / Rrim(I) all: 0.08 / Net I/σ(I): 13.42
Reflection shellResolution: 1.8→1.85 Å / Redundancy: 4.03 % / Mean I/σ(I) obs: 0.96 / Num. unique obs: 14146 / CC1/2: 0.32 / Rrim(I) all: 1.844 / % possible all: 88

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Processing

Software
NameVersionClassification
PHENIX1.21_5207refinement
XDSVERSION Jun 30, 2023 BUILT=20230630data reduction
XSCALEVERSION Jun 30, 2023 BUILT=20230630data scaling
PHENIXphasing
RefinementMethod to determine structure: FOURIER SYNTHESIS / Resolution: 1.8→47.38 Å / SU ML: 0.2382 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 19.0965
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.1769 10728 5 %
Rwork0.1541 203831 -
obs0.1553 214559 98.79 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 37.98 Å2
Refinement stepCycle: LAST / Resolution: 1.8→47.38 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms16795 0 579 1502 18876
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.005318036
X-RAY DIFFRACTIONf_angle_d0.857924616
X-RAY DIFFRACTIONf_chiral_restr0.04962575
X-RAY DIFFRACTIONf_plane_restr0.00793130
X-RAY DIFFRACTIONf_dihedral_angle_d12.91396475
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AAX-RAY DIFFRACTIONTorsion NCS0.480651956202
ens_1d_3AAX-RAY DIFFRACTIONTorsion NCS0.469623668511
ens_1d_4AAX-RAY DIFFRACTIONTorsion NCS0.469597641789
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.8-1.820.39723070.37245871X-RAY DIFFRACTION85.4
1.82-1.840.34983230.33936127X-RAY DIFFRACTION89.26
1.84-1.860.36963390.32326436X-RAY DIFFRACTION94.24
1.86-1.890.32913520.28736697X-RAY DIFFRACTION96.72
1.89-1.910.28173590.27476818X-RAY DIFFRACTION99.36
1.91-1.940.28473610.25376844X-RAY DIFFRACTION99.97
1.94-1.970.28043610.23286862X-RAY DIFFRACTION99.99
1.97-20.22993580.20666797X-RAY DIFFRACTION99.99
2-2.030.23113620.19686876X-RAY DIFFRACTION99.97
2.03-2.060.22423610.18486864X-RAY DIFFRACTION99.99
2.06-2.10.20023610.18056856X-RAY DIFFRACTION99.99
2.1-2.130.20623620.17556880X-RAY DIFFRACTION100
2.13-2.180.19393610.17466863X-RAY DIFFRACTION100
2.18-2.220.20573580.16846799X-RAY DIFFRACTION99.97
2.22-2.270.19173620.15756878X-RAY DIFFRACTION100
2.27-2.320.16593620.14476881X-RAY DIFFRACTION99.99
2.32-2.380.17823610.14416856X-RAY DIFFRACTION99.99
2.38-2.440.17993630.13786895X-RAY DIFFRACTION100
2.44-2.510.17163600.1386842X-RAY DIFFRACTION99.99
2.51-2.60.17963630.13946885X-RAY DIFFRACTION100
2.6-2.690.16583600.14226852X-RAY DIFFRACTION99.93
2.69-2.80.17773620.15236885X-RAY DIFFRACTION100
2.8-2.920.20513630.16856897X-RAY DIFFRACTION100
2.92-3.080.18643630.15886889X-RAY DIFFRACTION100
3.08-3.270.17873630.15046902X-RAY DIFFRACTION99.99
3.27-3.520.17283610.1456846X-RAY DIFFRACTION99.88
3.52-3.880.14163640.12716922X-RAY DIFFRACTION99.89
3.88-4.440.12793620.10996887X-RAY DIFFRACTION99.92
4.44-5.590.1263640.11846917X-RAY DIFFRACTION99.77
5.59-47.380.17663700.17027007X-RAY DIFFRACTION99.65
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.7127614146840.0546023249898-0.08701882811460.586221776814-0.04070067682060.6405322775250.0363059340569-0.0190287117097-0.0284058952349-0.0399376602179-0.0498249102676-0.1132345735520.02028174735210.194043498176-3.7420806271E-50.2644033941470.03413692386290.01478397066810.3263864044160.02100814042550.28209890838939.4920791018-5.2445600921932.3008251598
20.1811186200020.002267830853150.07575439246610.157395441125-0.02763572907360.172677551050.109517099081-0.0723822536489-0.1621832677120.0153007221329-0.0695895961155-0.1737486499380.09278187921830.190421975395-0.0001551933206030.2952417497060.0123792677692-0.00933452216370.3743793622080.01228230995820.34112778816338.1196606095-6.4999356973831.210498511
30.267870576269-0.00826759134490.03064888894470.2904255748460.3016049515750.318697611220.0698083015671-0.2122308026940.6068289381060.168307204768-0.126351765109-0.441088772512-0.315288871060.209978929389-8.82481915408E-50.415605032505-0.07982451347460.02197169382620.448369613294-0.03462987385360.52266314814438.942927920518.933568614648.4906350793
40.7920748214170.181729133488-0.1592727142350.769871152482-0.03615127224680.6468101394860.0415846573843-0.116654323315-0.001560005704920.00392970953432-0.0567187613878-0.0671788781336-0.02859076392480.1175897344992.065786248E-60.256411315467-0.00229999657458-0.01119194858870.3263018162990.008063652219030.28165190550534.1076639913-0.82319933408441.6302197557
50.597629286830.0636559846732-0.1487774741230.645116859745-0.1040755971140.7321086583410.05475081410270.08502325515390.0524860848375-0.0427284969551-0.04600993827860.110404055147-0.0439374230453-0.1923505505630.0001045430601220.2586850558310.0284370209802-0.03707928391990.3244960536-0.01765504006660.2781736253390.732372922129-2.1189438644932.03372263
60.665741249065-0.175174706316-0.09672986074470.2147571578750.2194665423830.4005477307960.07233019280570.06695706680210.0916620828145-0.0355543441081-0.04604962008840.123904909220.0128631650865-0.21506074367-0.0002468730467330.265192072224-0.0103565545396-0.01009957519850.328308720112-0.02902476042870.303762921269-0.756128136162-4.9944771738336.5399589655
70.2354143932570.1827556598190.09566379498150.50280163763-0.349680351560.537161921606-0.0866843485735-0.244624278911-0.3440006782090.384990804816-0.1578954657510.2714861480720.388286724266-0.1872835887950.0006317151231620.41951046236-0.0458937001281-0.00426197463850.3401883019010.01940230856940.4425164029546.92600978965-31.600201377950.2407769655
80.5514048303710.158450821616-0.1356284106860.6562055692440.02526337083130.7977999758650.0316081512805-0.03847603790140.02584725217430.00276561566643-0.03254898812320.0403478978958-0.00867948022304-0.0807175254514-1.74072269995E-50.2609210608340.00538207123556-0.01546618675430.303866205182-0.0143851702730.2839332585446.01458397853-5.0206150447941.211942647
90.690691125881-0.1751999939530.055237523231.246425052830.01055120626050.944094205669-0.06428476627850.1590534638620.117115039122-0.05743491048550.00941200010974-0.276101881295-0.2429523250710.215369583756-0.0003768143691890.384071641952-0.03635992875330.004174712529680.386901921470.06158686947830.3432022809520.237598233412.117688507584.3122705062
100.175992522369-0.02131083063030.0988966809920.1465507713810.156473366550.252342442564-0.05184896468310.1557369371070.04776613896640.0382498844276-0.0106153250411-0.295469674043-0.1501256142370.321056508067-0.0003149307863370.383683434369-0.0346464607531-0.02176284768250.4144223865380.04859908036490.34087513051320.647071753111.564111675182.8116196543
110.188892450813-0.0970272307659-0.2897574713080.06602728301540.1500278676440.44364380266-0.0940508609429-0.04210558704030.3358648842850.3759666754310.01010194024290.204479175816-0.350628314894-0.2061145633480.003231593557170.5218889633790.1190760372080.004467006362170.3030974874830.0257997084330.389926236298-2.8557538577221.118071198399.8984306455
120.569278686661-0.2401092928650.006912455466191.4159790072-0.1331783196180.855480691808-0.07887732629140.08121558887370.05301629203640.1650851876870.0203162557653-0.120211806646-0.1640002491460.06649226239388.05597709868E-50.36215847570.0115313456487-0.01562826238580.3145784669880.01429989181570.26390877855313.37749493159.35187883393.6199139154
130.5879732795570.029266714346-0.2015300140080.982476942234-0.3374048073090.831108168243-0.0855757710794-0.017517741424-0.2161756713560.2255526355110.06429058824630.2973174794250.366717114235-0.125060959818-0.0002341308107810.4908174246980.01389758981080.08636526493130.264509530141-0.03506251137240.3747388685736.15972218602-32.260837389396.8611034331
140.447423497386-0.085795108230.206717642950.427135905990.06700985120.885203683827-0.002435593072910.189226299935-0.147511209086-0.2362857482-0.004992290989650.2745572985010.137236513015-0.1757818418263.62406033563E-50.330119029331-0.0123396230379-0.0004257596542710.401658414431-0.05154831291910.377021979082-3.68616084104-13.155664316573.3896194424
150.190479122457-0.149084559006-0.1009939702530.211769793691-0.05805060456150.245732421438-0.002265672448150.189419569736-0.160670392841-0.08962550091730.0002264742487950.3729869713930.114518288774-0.183819017173-0.0004271740822220.389708742928-0.01278123208140.007199352097480.388654816074-0.06054381722030.4066016166970.376794566865-21.012255936182.3369979691
160.4662902648980.09774514507530.1940567010250.4330920992370.006979563213860.527587232425-0.133850026026-0.0586881622945-0.39080022820.4422351161420.0756446506666-0.2197905179180.4161937649560.2802508101020.02226930191220.5131109117770.1145933295410.05584880872590.311961138123-0.01189108464830.38856880253521.9714006541-30.9680123352101.988935164
170.601793887718-0.6660135679980.02336130475671.674532278420.006911789399330.845831603662-0.07030707338340.0387408435775-0.1297491140910.1858252069990.03862517096110.2107896829370.131102179279-0.0577616881145-0.003019476728430.3566301202990.01224896814730.05654077629220.323068245642-0.01432336714310.3258295207016.81653846727-18.500011148693.7302805718
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 61 through 272 )AA61 - 2721 - 212
22chain 'A' and (resid 273 through 308 )AA273 - 308213 - 245
33chain 'A' and (resid 309 through 348 )AA309 - 348246 - 285
44chain 'A' and (resid 349 through 599 )AA349 - 599286 - 536
55chain 'B' and (resid 65 through 272 )BD65 - 2721 - 208
66chain 'B' and (resid 273 through 321 )BD273 - 321209 - 254
77chain 'B' and (resid 322 through 360 )BD322 - 360255 - 293
88chain 'B' and (resid 361 through 598 )BD361 - 598294 - 531
99chain 'C' and (resid 64 through 272 )CG64 - 2721 - 209
1010chain 'C' and (resid 273 through 308 )CG273 - 308210 - 245
1111chain 'C' and (resid 309 through 348 )CG309 - 348246 - 281
1212chain 'C' and (resid 349 through 599 )CG349 - 599282 - 532
1313chain 'D' and (resid 64 through 158 )DJ64 - 1581 - 95
1414chain 'D' and (resid 159 through 272 )DJ159 - 27296 - 209
1515chain 'D' and (resid 273 through 308 )DJ273 - 308210 - 245
1616chain 'D' and (resid 309 through 348 )DJ309 - 348246 - 285
1717chain 'D' and (resid 349 through 598 )DJ349 - 598286 - 535

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