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- PDB-9eni: L-amino acid oxidase 4 (HcLAAO4) from the fungus Hebeloma cylindr... -

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Basic information

Entry
Database: PDB / ID: 9eni
TitleL-amino acid oxidase 4 (HcLAAO4) from the fungus Hebeloma cylindrosporum in complex with L-glutamine
ComponentsL-amino acid oxidase 4
KeywordsOXIDOREDUCTASE / L-AMINO ACID OXIDASE / FAD / FLAVOPROTEIN
Function / homology
Function and homology information


L-amino-acid oxidase activity / amino acid catabolic process
Similarity search - Function
: / Amine oxidase / Flavin containing amine oxidoreductase / FAD/NAD(P)-binding domain superfamily
Similarity search - Domain/homology
DIHYDROFLAVINE-ADENINE DINUCLEOTIDE / GLUTAMINE / S-1,2-PROPANEDIOL / R-1,2-PROPANEDIOL / L-amino acid oxidase 4
Similarity search - Component
Biological speciesHebeloma cylindrosporum (fungus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å
AuthorsGilzer, D. / Koopmeiners, S. / Fischer von Mollard, G. / Niemann, H.H.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: Febs Lett. / Year: 2024
Title: Crystal structure and enzyme engineering of the broad substrate spectrum l-amino acid oxidase 4 from the fungus Hebeloma cylindrosporum.
Authors: Koopmeiners, S. / Gilzer, D. / Widmann, C. / Berelsmann, N. / Spross, J. / Niemann, H.H. / Fischer von Mollard, G.
History
DepositionMar 13, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 14, 2024Provider: repository / Type: Initial release
Revision 1.1Aug 28, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.2Oct 9, 2024Group: Database references / Structure summary / Category: citation / citation_author / pdbx_entry_details
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID / _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: L-amino acid oxidase 4
B: L-amino acid oxidase 4
C: L-amino acid oxidase 4
D: L-amino acid oxidase 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)257,76269
Polymers248,7914
Non-polymers8,97165
Water20,3391129
1
A: L-amino acid oxidase 4
B: L-amino acid oxidase 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)129,17738
Polymers124,3952
Non-polymers4,78236
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: L-amino acid oxidase 4
D: L-amino acid oxidase 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)128,58431
Polymers124,3952
Non-polymers4,18929
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)84.710, 131.740, 107.250
Angle α, β, γ (deg.)90.000, 95.949, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 65 through 83 or resid 85...
d_2ens_1(chain "B" and (resid 65 through 83 or resid 85...
d_3ens_1(chain "C" and (resid 65 through 83 or resid 85...
d_4ens_1(chain "D" and (resid 65 through 83 or resid 85...

NCS domain segments:

Ens-ID: ens_1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11GLUGLULEULEUAA65 - 8312 - 30
d_12SERSERGLYGLYAA85 - 14432 - 91
d_13LEULEUVALVALAA146 - 20593 - 152
d_14ASPASPPROPROAA207 - 249154 - 196
d_15PROPROSERSERAA251 - 257198 - 204
d_16HISHISLYSLYSAA259 - 322206 - 269
d_17ALAALAALAALAAA327 - 332274 - 279
d_18VALVALLYSLYSAA334 - 357281 - 304
d_19LYSLYSALAALAAA359 - 475306 - 422
d_110TYRTYRGLUGLUAA477 - 478424 - 425
d_111GLNGLNLEULEUAA480 - 486427 - 433
d_112ASNASNVALVALAA488 - 505435 - 452
d_113VALVALASNASNAA507 - 513454 - 460
d_114ASNASNPHEPHEAA515 - 530462 - 477
d_115ASPASPGLUGLUAA532 - 598479 - 545
d_117GLNGLNGLNGLNAF702
d_21GLUGLULEULEUBB65 - 8312 - 30
d_22SERSERGLYGLYBB85 - 14432 - 91
d_23LEULEUVALVALBB146 - 20593 - 152
d_24ASPASPPROPROBB207 - 249154 - 196
d_25PROPROSERSERBB251 - 257198 - 204
d_26HISHISGLUGLUBB259 - 294206 - 241
d_27GLYGLYLYSLYSBB298 - 322245 - 269
d_28ALAALAALAALABB327 - 332274 - 279
d_29VALVALLYSLYSBB334 - 357281 - 304
d_210LYSLYSALAALABB359 - 475306 - 422
d_211TYRTYRGLUGLUBB477 - 478424 - 425
d_212GLNGLNLEULEUBB480 - 486427 - 433
d_213ASNASNVALVALBB488 - 505435 - 452
d_214VALVALASNASNBB507 - 513454 - 460
d_215ASNASNPHEPHEBB515 - 530462 - 477
d_216ASPASPGLUGLUBB532 - 598479 - 545
d_218GLNGLNGLNGLNBZ702
d_31GLUGLULEULEUCC65 - 8312 - 30
d_32SERSERGLYGLYCC85 - 14432 - 91
d_33LEULEUVALVALCC146 - 20593 - 152
d_34ASPASPPROPROCC207 - 249154 - 196
d_35PROPROSERSERCC251 - 257198 - 204
d_36HISHISGLUGLUCC259 - 294206 - 241
d_37GLYGLYALAALACC298 - 332245 - 279
d_38VALVALLYSLYSCC334 - 357281 - 304
d_39LYSLYSALAALACC359 - 475306 - 422
d_310TYRTYRGLUGLUCC477 - 478424 - 425
d_311GLNGLNLEULEUCC480 - 486427 - 433
d_312ASNASNVALVALCC488 - 505435 - 452
d_313VALVALASNASNCC507 - 513454 - 460
d_314ASNASNPHEPHECC515 - 530462 - 477
d_315ASPASPGLUGLUCC532 - 598479 - 545
d_317GLNGLNGLNGLNCPA702
d_41GLUGLULEULEUDD65 - 8312 - 30
d_42SERSERGLYGLYDD85 - 14432 - 91
d_43LEULEUVALVALDD146 - 20593 - 152
d_44ASPASPPROPRODD207 - 249154 - 196
d_45PROPROSERSERDD251 - 257198 - 204
d_46HISHISGLUGLUDD259 - 294206 - 241
d_47GLYGLYLYSLYSDD298 - 322245 - 269
d_48ALAALAALAALADD327 - 332274 - 279
d_49VALVALLYSLYSDD334 - 357281 - 304
d_410LYSLYSALAALADD359 - 475306 - 422
d_411TYRTYRGLUGLUDD477 - 478424 - 425
d_412GLNGLNLEULEUDD480 - 486427 - 433
d_413ASNASNVALVALDD488 - 505435 - 452
d_414VALVALASNASNDD507 - 513454 - 460
d_415ASNASNPHEPHEDD515 - 530462 - 477
d_416ASPASPGLUGLUDD532 - 598479 - 545
d_418GLNGLNGLNGLNDEB702

NCS oper:
IDCodeMatrixVector
1given(-0.999960874687, 0.00535644536848, -0.00703971509605), (-0.00586253606241, -0.997244350946, 0.0739549536982), (-0.00662418044259, 0.0739933307711, 0.997236735803)40.3676616632, -9.95376814549, 0.502108430777
2given(0.418710724577, -0.908092077663, -0.00707867296078), (0.90809073525, 0.418746885392, -0.00471831833878), (0.00724883975837, -0.00445246684313, 0.999963814276)-1.25097208239, -21.0762120913, 51.7187897591
3given(-0.41615293445, 0.905599583975, -0.0818909558696), (-0.909227353147, -0.415526733471, 0.0253604822449), (-0.011061439223, 0.0850113361573, 0.996318582225)24.8763593901, 11.1010865211, 52.5566569815

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Components

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Protein , 1 types, 4 molecules ABCD

#1: Protein
L-amino acid oxidase 4


Mass: 62197.676 Da / Num. of mol.: 4 / Mutation: K474A, K475A
Source method: isolated from a genetically manipulated source
Details: residues 54 - 615 obtained by limited proteolysis; surface entropy reduction mutations K474A and K475A
Source: (gene. exp.) Hebeloma cylindrosporum (fungus) / Plasmid: pET28b / Production host: Escherichia coli (E. coli) / Strain (production host): Arctic Express / References: UniProt: S4S6Z0, L-amino-acid oxidase

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Non-polymers , 6 types, 1194 molecules

#2: Chemical
ChemComp-FDA / DIHYDROFLAVINE-ADENINE DINUCLEOTIDE


Mass: 787.566 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C27H35N9O15P2 / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical
ChemComp-GLN / GLUTAMINE


Type: L-peptide linking / Mass: 146.144 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C5H10N2O3 / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical...
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 45 / Source method: obtained synthetically / Formula: SO4
#5: Chemical
ChemComp-PGR / R-1,2-PROPANEDIOL


Mass: 76.094 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C3H8O2
#6: Chemical ChemComp-PGO / S-1,2-PROPANEDIOL


Mass: 76.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C3H8O2
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1129 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.39 Å3/Da / Density % sol: 48.59 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.1
Details: protein: 10 mg/ml; reservoir solution: 2.1 M (NH4)2SO4, 0.1 M sodium citrate, pH 7.1; drop with 1000 nl protein + 500 nl reservoir

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: MASSIF-3 / Wavelength: 0.9677 Å
DetectorType: DECTRIS EIGER X 4M / Detector: PIXEL / Date: Feb 22, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9677 Å / Relative weight: 1
ReflectionResolution: 2.1→50 Å / Num. obs: 127166 / % possible obs: 93.3 % / Redundancy: 7.57 % / Biso Wilson estimate: 40.56 Å2 / CC1/2: 0.998 / Rrim(I) all: 0.084 / Net I/σ(I): 15.86
Reflection shellResolution: 2.1→2.15 Å / Redundancy: 7.73 % / Mean I/σ(I) obs: 1.64 / Num. unique obs: 9532 / CC1/2: 0.636 / Rrim(I) all: 1.629 / % possible all: 95

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Processing

Software
NameVersionClassification
PHENIX1.21_5207refinement
XDSVERSION Jun 30, 2023 BUILT=20230630data reduction
XSCALEVERSION Jun 30, 2023 BUILT=20230630data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.1→42.13 Å / SU ML: 0.2874 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 21.3479
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2026 6353 5 %
Rwork0.1715 120752 -
obs0.173 127105 93.33 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 45.16 Å2
Refinement stepCycle: LAST / Resolution: 2.1→42.13 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms16798 0 537 1129 18464
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002817949
X-RAY DIFFRACTIONf_angle_d0.562424491
X-RAY DIFFRACTIONf_chiral_restr0.04132562
X-RAY DIFFRACTIONf_plane_restr0.00443117
X-RAY DIFFRACTIONf_dihedral_angle_d11.41886437
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AAX-RAY DIFFRACTIONTorsion NCS0.397949992132
ens_1d_3AAX-RAY DIFFRACTIONTorsion NCS0.399988623579
ens_1d_4AAX-RAY DIFFRACTIONTorsion NCS0.439944682797
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.1-2.120.4212160.39714107X-RAY DIFFRACTION95.18
2.12-2.150.34082140.33344055X-RAY DIFFRACTION94.72
2.15-2.180.36052140.28864094X-RAY DIFFRACTION94.93
2.18-2.20.27332140.25694053X-RAY DIFFRACTION94.82
2.2-2.230.29812160.24344115X-RAY DIFFRACTION94.4
2.23-2.260.2682100.2523991X-RAY DIFFRACTION94.75
2.26-2.290.30862140.26994088X-RAY DIFFRACTION94.34
2.29-2.330.31982130.26564029X-RAY DIFFRACTION94.06
2.33-2.370.29062140.25084067X-RAY DIFFRACTION94.59
2.37-2.40.26352130.22274055X-RAY DIFFRACTION94.11
2.4-2.450.24162130.1964044X-RAY DIFFRACTION93.7
2.45-2.490.22912130.18594047X-RAY DIFFRACTION93.52
2.49-2.540.24312090.17733970X-RAY DIFFRACTION93.41
2.54-2.590.22112110.17844003X-RAY DIFFRACTION93.11
2.59-2.650.23072110.18154023X-RAY DIFFRACTION92.57
2.65-2.710.23682060.17863916X-RAY DIFFRACTION91.97
2.71-2.770.21192060.18853917X-RAY DIFFRACTION90.94
2.77-2.850.23942050.19583908X-RAY DIFFRACTION90.1
2.85-2.930.23762000.18863800X-RAY DIFFRACTION88.13
2.93-3.030.24591940.19153681X-RAY DIFFRACTION85.31
3.03-3.140.21541690.18373219X-RAY DIFFRACTION74.27
3.14-3.260.20421730.18083279X-RAY DIFFRACTION76.3
3.26-3.410.21092220.17414218X-RAY DIFFRACTION97.52
3.41-3.590.18682220.15654226X-RAY DIFFRACTION98.63
3.59-3.820.16592270.14844309X-RAY DIFFRACTION99.13
3.82-4.110.14562240.12254259X-RAY DIFFRACTION98.98
4.11-4.520.14632260.1234295X-RAY DIFFRACTION99.1
4.52-5.180.14612260.11864295X-RAY DIFFRACTION99.28
5.18-6.520.17532290.15014354X-RAY DIFFRACTION99.35
6.52-42.130.17442290.17014335X-RAY DIFFRACTION98.49
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.6775361071120.0291447262434-0.1664522204960.6096069297530.03752054424010.6535841260.0325023463383-0.0224594466378-0.0564075225191-0.0604330161676-0.0473897721375-0.07730098547650.0182403963330.162447128685.31548988827E-70.3736969656070.02354854253130.00180257524330.4591951651340.02346755717880.40357350715239.4936803836-4.9807372207932.3752967194
20.220175600991-0.1485312726480.2113156150230.103904263999-0.1528495566840.3649672607560.127178776869-0.0645507596022-0.160028061780.00237353701702-0.089005576983-0.05080253361560.1325764171910.0809766578341-2.59661057696E-60.4076264982850.00703582373925-0.007701827770480.4673239619970.01565021068890.46202125738338.3391737088-6.4463294274531.3995953276
30.5399523537440.044404594688-0.2419411109130.330774851750.3354127561950.6091472851880.141213818849-0.1498225852970.2662571770970.0448168713818-0.118940433213-0.095237846075-0.1908251459030.138852085169-1.92209495063E-60.443002928668-0.1026133294280.02227777287630.491176945847-0.03301080510430.49456054781338.869472381418.88518377548.4390430029
40.8300102688030.102589851525-0.1656437274980.774135048694-0.04510487362760.5835034668910.0437094640951-0.116175347925-0.006803599343370.0186562633703-0.0536487308314-0.0554633856914-0.03623861701120.1102199705293.15502290257E-70.368334835546-0.0137161280602-0.01907312352630.4465514187050.006804477110030.38702889399834.0073429232-0.79879772289641.6648547951
50.5269438417580.0898823145605-0.1305695629760.664335232597-0.1196338170760.6444435916510.05021848637930.09882830811610.0465594392601-0.0530896022883-0.0558765593840.0823926515364-0.0248348368022-0.168524579804-6.10732804218E-50.3713800770210.0208509016654-0.03757274637340.470233175173-0.01858640070390.4235089062010.716725941387-2.139363580232.1037867565
60.302983595358-0.0892042530523-0.3114991116990.08247535191940.01538035636330.4139416949320.08376090681390.1537237745160.222010985961-0.0715585800853-0.03917536232990.085667080894-0.0916280670494-0.3101019912853.51433977507E-50.402087054720.00110657066952-0.0243941893130.49848128074-0.01807661624510.4604976804581.02162554226-1.5597031201130.4099531067
70.164327698683-0.1839438623220.2067022056970.617509470054-0.2962229961460.738963804172-0.0953835313351-0.263553420425-0.3318952999840.3625470064450.03641513287190.1026678043030.3231646133210.0451826937103-0.000749788223410.396850341892-0.07594180295410.007466756032560.41615530455-0.009274476331470.4935634470131.78170915386-25.50934319849.6890950785
80.5514168866550.215338213701-0.2093543178160.6880921502860.09558796474620.7102678524150.0319892128618-0.0554242459012-0.0131133569264-0.00293133629903-0.01740108255130.0499432112475-0.00524007675277-0.05675276908847.36807843915E-60.371558072267-0.00901423512649-0.03056103762630.42457951133-0.01166245521890.4070409300236.14697190916-6.2486829506841.7442484189
90.683965016566-0.2688905552920.03404690957031.33245738505-0.05917745670240.971220587276-0.07478703266280.162120166980.112553749116-0.0668285727795-0.0166808879028-0.250557320713-0.2222271162020.1903486739113.98600129895E-50.458853716295-0.04794704355410.007875181056360.5065122221590.06213523467430.45783612472920.172307203412.103688474784.3591537027
100.173511544347-0.006290660239980.0705215305710.1893584853190.244157097750.348642133217-0.0753360492420.236369888719-0.03315119919470.01754961329760.0640799167105-0.20858278565-0.122853899550.3373008750087.71819304646E-50.46226600148-0.0398245159428-0.03366059663050.4977749934830.06154384074890.46942520722820.741601314711.628594057782.7501036732
110.411077006574-0.159399743454-0.4896158240740.2975829364240.08870544334010.625313406755-0.218816260896-0.05469016344520.2316783047530.3299687933470.1023539967810.197366006901-0.325135306677-0.159935295603-3.09400093745E-50.5772594439220.115783428501-0.02421164927810.4268961299130.03596679195960.510528118289-2.9223925444520.865795277799.8269782113
120.28728191121-0.06639555168610.0428516950980.311992991659-0.1677850488990.271442167184-0.05385565953480.1073566861420.04674453154440.128845065191-0.0479786044970.390445480551-0.0549208551604-0.218151889308-2.58195563704E-50.4579354359370.09704501909050.04194585470740.4985071937320.00932405878380.496237436639-8.0479316411111.416624437792.8746794068
130.889066791757-0.2203179737040.08848360753621.24549244356-0.1088458287810.933669149247-0.0541101056342-0.008155632004240.04306713088270.175891771829-0.0181011787262-0.234472660427-0.08320675483660.139261566611-0.0001371351336520.439582083565-0.00065024406641-0.02958358371320.4430756148050.02281995425110.41882922279321.38437829563.9904207062696.18247671
140.209884207839-0.114656786450.2066589019740.271957460494-0.1483205723970.25979952569-0.1248190955870.1735479588770.179808293829-0.01477409561550.02101204327590.126541635974-0.110315577359-0.012481689296-0.0001431695205810.5671271756120.053859375758-0.03645077673870.4952866676040.05540928212940.502786497883.0618621272228.607450755983.5741192585
150.699697312959-0.3292912974680.1144585945491.09889277407-0.01451362360221.04320157659-0.03354661885430.0577316154072-0.1690737779450.02289073503040.006365259381940.2263758869910.229467933814-0.1112913601293.13287244443E-50.410052988324-0.04146246052570.04661194016030.437352343979-0.0508988891920.4736847823810.756717531689-21.576754071583.8629592117
160.402366049629-0.247239279572-0.0932118143180.26269917101-0.1299377438770.332518296742-0.02750485656940.049739165226-0.145040368782-0.107592981594-0.05271326198590.4492020926140.203316582428-0.2220044140424.6592581935E-50.535384569086-0.0126598690376-0.004362800648980.517613113831-0.05049762269880.5464434452930.22705792083-21.083293595782.2075614985
170.401035115074-0.1632332744510.406089677280.373696380094-0.069592988250.479511741851-0.15165477027-0.175172164594-0.5490947109980.378235710980.0550959454062-0.1689090653630.3215752308630.212339140289-1.35206526911E-50.5974483513990.1226315956410.05461877535830.474396927146-0.003976608496710.57393056866121.795684063-30.8231078507101.986810508
180.844577537982-0.6683860173350.004516285898991.6885231233-0.01846383737460.886023285172-0.067105394410.0181926213731-0.1175918141950.1661813074280.02769725808430.1958864424460.114558590015-0.0450428131106-6.83659949702E-50.4376714564880.01062092679860.05346013022170.433011638464-0.01144063591040.428914414036.72101589768-18.412197103593.6914188592
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 60 through 272 )AA60 - 2721 - 213
22chain 'A' and (resid 273 through 308 )AA273 - 308214 - 246
33chain 'A' and (resid 309 through 348 )AA309 - 348247 - 286
44chain 'A' and (resid 349 through 599 )AA349 - 599287 - 537
55chain 'B' and (resid 65 through 272 )BD65 - 2721 - 208
66chain 'B' and (resid 273 through 308 )BD273 - 308209 - 244
77chain 'B' and (resid 309 through 348 )BD309 - 348245 - 281
88chain 'B' and (resid 349 through 598 )BD349 - 598282 - 531
99chain 'C' and (resid 64 through 272 )CG64 - 2721 - 209
1010chain 'C' and (resid 273 through 308 )CG273 - 308210 - 245
1111chain 'C' and (resid 309 through 348 )CG309 - 348246 - 281
1212chain 'C' and (resid 349 through 392 )CG349 - 392282 - 325
1313chain 'C' and (resid 393 through 559 )CG393 - 559326 - 492
1414chain 'C' and (resid 560 through 599 )CG560 - 599493 - 532
1515chain 'D' and (resid 64 through 272 )DJ64 - 2721 - 209
1616chain 'D' and (resid 273 through 308 )DJ273 - 308210 - 245
1717chain 'D' and (resid 309 through 348 )DJ309 - 348246 - 285
1818chain 'D' and (resid 349 through 598 )DJ349 - 598286 - 535

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