+
Open data
-
Basic information
Entry | Database: PDB / ID: 9emb | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Structure of KefC Asp156Asn variant | |||||||||
![]() | Glutathione-regulated potassium-efflux system protein KefC | |||||||||
![]() | TRANSPORT PROTEIN / KefC / Potassium / GSH / Mutant | |||||||||
Function / homology | ![]() glutathione-regulated potassium exporter activity / response to methylglyoxal / potassium:proton antiporter complex / intracellular pH elevation / regulation of pH / antiporter activity / toxic substance binding / proton transmembrane transport / regulation of intracellular pH / potassium ion transport ...glutathione-regulated potassium exporter activity / response to methylglyoxal / potassium:proton antiporter complex / intracellular pH elevation / regulation of pH / antiporter activity / toxic substance binding / proton transmembrane transport / regulation of intracellular pH / potassium ion transport / response to hydrogen peroxide / response to toxic substance / nucleotide binding / enzyme binding / protein homodimerization activity / membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.98 Å | |||||||||
![]() | Gulati, A. / Kokane, S. / Drew, D. | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Structure of KefC Asp156Asn variant Authors: Gulati, A. / Kokane, S. / Drew, D. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 514.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 342.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.5 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.5 MB | Display | |
Data in XML | ![]() | 43.1 KB | Display | |
Data in CIF | ![]() | 63.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 19816MC M: map data used to model this data C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
|
---|---|
1 |
|
-
Components
#1: Protein | Mass: 61182.238 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Chemical | Has ligand of interest | Y | |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-
Sample preparation
Component | Name: Homodimer of KefC Asp156Asn variant / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
---|---|
Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 600 nm |
Image recording | Electron dose: 54.6 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-
Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
3D reconstruction | Resolution: 2.98 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 327001 / Symmetry type: POINT | ||||||||||||||||||||||||
Refinement | Cross valid method: NONE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2 | ||||||||||||||||||||||||
Displacement parameters | Biso mean: 115.78 Å2 | ||||||||||||||||||||||||
Refine LS restraints |
|