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- PDB-9ek4: Crystal structure of HLA-B*07:02 with the 9-mer TP53 mutant pepti... -

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Basic information

Entry
Database: PDB / ID: 9ek4
TitleCrystal structure of HLA-B*07:02 with the 9-mer TP53 mutant peptide MPILTIITL
Components
  • Beta-2-microglobulin
  • HLA class I histocompatibility antigen, B alpha chain
  • Mutant peptide from Cellular tumor antigen p53
KeywordsIMMUNE SYSTEM / HLA-B:07:02 / TP53 / neoantigen
Function / homology
Function and homology information


regulation of interleukin-12 production / regulation of dendritic cell differentiation / regulation of T cell anergy / negative regulation of helicase activity / Loss of function of TP53 in cancer due to loss of tetramerization ability / Regulation of TP53 Expression / signal transduction by p53 class mediator / negative regulation of G1 to G0 transition / negative regulation of glucose catabolic process to lactate via pyruvate / Transcriptional activation of cell cycle inhibitor p21 ...regulation of interleukin-12 production / regulation of dendritic cell differentiation / regulation of T cell anergy / negative regulation of helicase activity / Loss of function of TP53 in cancer due to loss of tetramerization ability / Regulation of TP53 Expression / signal transduction by p53 class mediator / negative regulation of G1 to G0 transition / negative regulation of glucose catabolic process to lactate via pyruvate / Transcriptional activation of cell cycle inhibitor p21 / regulation of intrinsic apoptotic signaling pathway by p53 class mediator / negative regulation of pentose-phosphate shunt / Activation of NOXA and translocation to mitochondria / ATP-dependent DNA/DNA annealing activity / regulation of cell cycle G2/M phase transition / regulation of interleukin-6 production / oligodendrocyte apoptotic process / negative regulation of miRNA processing / intrinsic apoptotic signaling pathway in response to hypoxia / oxidative stress-induced premature senescence / regulation of tissue remodeling / positive regulation of thymocyte apoptotic process / positive regulation of mitochondrial membrane permeability / germ cell nucleus / regulation of fibroblast apoptotic process / bone marrow development / cellular response to actinomycin D / circadian behavior / histone deacetylase regulator activity / positive regulation of programmed necrotic cell death / : / regulation of mitochondrial membrane permeability involved in apoptotic process / RUNX3 regulates CDKN1A transcription / T cell proliferation involved in immune response / TP53 Regulates Transcription of Death Receptors and Ligands / Activation of PUMA and translocation to mitochondria / TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain / mRNA transcription / negative regulation of glial cell proliferation / negative regulation of neuroblast proliferation / regulation of DNA damage response, signal transduction by p53 class mediator / Regulation of TP53 Activity through Association with Co-factors / Formation of Senescence-Associated Heterochromatin Foci (SAHF) / mitochondrial DNA repair / T cell lineage commitment / thymocyte apoptotic process / ER overload response / TP53 Regulates Transcription of Caspase Activators and Caspases / cardiac septum morphogenesis / B cell lineage commitment / entrainment of circadian clock by photoperiod / negative regulation of DNA replication / Zygotic genome activation (ZGA) / negative regulation of mitophagy / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / necroptotic process / PI5P Regulates TP53 Acetylation / Association of TriC/CCT with target proteins during biosynthesis / positive regulation of release of cytochrome c from mitochondria / negative regulation of telomere maintenance via telomerase / SUMOylation of transcription factors / TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain / protection from natural killer cell mediated cytotoxicity / rRNA transcription / negative regulation of reactive oxygen species metabolic process / intrinsic apoptotic signaling pathway by p53 class mediator / TFIID-class transcription factor complex binding / Transcriptional Regulation by VENTX / cellular response to UV-C / viral process / neuroblast proliferation / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / TAP binding / replicative senescence / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / Pyroptosis / positive regulation of RNA polymerase II transcription preinitiation complex assembly / chromosome organization / general transcription initiation factor binding / positive regulation of execution phase of apoptosis / hematopoietic stem cell differentiation / detection of bacterium / embryonic organ development / type II interferon-mediated signaling pathway / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest / response to X-ray / hematopoietic progenitor cell differentiation / somitogenesis / negative regulation of stem cell proliferation / core promoter sequence-specific DNA binding / glial cell proliferation / negative regulation of fibroblast proliferation / cis-regulatory region sequence-specific DNA binding / cellular response to glucose starvation / mitophagy / Regulation of TP53 Activity through Acetylation / negative regulation of proteolysis / mitotic G1 DNA damage checkpoint signaling
Similarity search - Function
Cellular tumor antigen p53, transactivation domain 2 / Transactivation domain 2 / p53 transactivation domain / P53 transactivation motif / : / p53 family signature. / p53, tetramerisation domain / P53 tetramerisation motif / p53, DNA-binding domain / P53 DNA-binding domain ...Cellular tumor antigen p53, transactivation domain 2 / Transactivation domain 2 / p53 transactivation domain / P53 transactivation motif / : / p53 family signature. / p53, tetramerisation domain / P53 tetramerisation motif / p53, DNA-binding domain / P53 DNA-binding domain / p53 tumour suppressor family / p53-like tetramerisation domain superfamily / p53/RUNT-type transcription factor, DNA-binding domain superfamily / p53-like transcription factor, DNA-binding / MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
ACETIC ACID / NICKEL (II) ION / HLA class I histocompatibility antigen, B alpha chain / Cellular tumor antigen p53 / Beta-2-microglobulin
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.05 Å
AuthorsTan, K. / Maillis, R.J. / Reinherz, E.L.
Funding support United States, 3items
OrganizationGrant numberCountry
National Institutes of Health/National Cancer Institute (NIH/NCI)R01CA265928 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)P01AI143565 United States
National Institutes of Health/Office of the DirectorS10OD030394 United States
CitationJournal: To Be Published
Title: Crystal structure of HLA-B*07:02 with the 9-mer TP53 mutant peptide MPILTIITL
Authors: Tan, K. / Maillis, R.J. / Reinherz, E.L.
History
DepositionNov 30, 2024Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 24, 2026Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: HLA class I histocompatibility antigen, B alpha chain
B: Beta-2-microglobulin
C: Mutant peptide from Cellular tumor antigen p53
D: HLA class I histocompatibility antigen, B alpha chain
E: Beta-2-microglobulin
F: Mutant peptide from Cellular tumor antigen p53
hetero molecules


Theoretical massNumber of molelcules
Total (without water)92,14037
Polymers90,1686
Non-polymers1,97231
Water4,792266
1
A: HLA class I histocompatibility antigen, B alpha chain
B: Beta-2-microglobulin
C: Mutant peptide from Cellular tumor antigen p53
hetero molecules


Theoretical massNumber of molelcules
Total (without water)46,21220
Polymers45,0843
Non-polymers1,12817
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6780 Å2
ΔGint-75 kcal/mol
Surface area19060 Å2
MethodPISA
2
D: HLA class I histocompatibility antigen, B alpha chain
E: Beta-2-microglobulin
F: Mutant peptide from Cellular tumor antigen p53
hetero molecules


Theoretical massNumber of molelcules
Total (without water)45,92817
Polymers45,0843
Non-polymers84414
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6350 Å2
ΔGint-65 kcal/mol
Surface area19120 Å2
MethodPISA
Unit cell
Length a, b, c (Å)46.984, 92.674, 108.548
Angle α, β, γ (deg.)90.000, 94.180, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

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Protein , 2 types, 4 molecules ADBE

#1: Protein HLA class I histocompatibility antigen, B alpha chain / Human leukocyte antigen B / HLA-B


Mass: 32190.324 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-B, HLAB / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P01889
#2: Protein Beta-2-microglobulin


Mass: 11879.356 Da / Num. of mol.: 2 / Fragment: UNP residues 21-119
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P61769

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Protein/peptide , 1 types, 2 molecules CF

#3: Protein/peptide Mutant peptide from Cellular tumor antigen p53 / Antigen NY-CO-13 / Phosphoprotein p53 / Tumor suppressor p53


Mass: 1014.322 Da / Num. of mol.: 2 / Fragment: residues 249-257 (Uniprot numbering) / Mutation: R249M (Uniprot numbering) / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P04637

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Non-polymers , 7 types, 297 molecules

#4: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: C2H6O2
#5: Chemical
ChemComp-ACY / ACETIC ACID


Mass: 60.052 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C2H4O2
#6: Chemical ChemComp-NI / NICKEL (II) ION


Mass: 58.693 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ni
#7: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: SO4
#8: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#9: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Cl
#10: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 266 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.61 Å3/Da / Density % sol: 52.94 %
Crystal growTemperature: 289 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: 0.18 M Ammonium Sulfate, 0.09 M Tris:HCl, 22.5% (w/v) PEG3350, 0.01 M Zinc Chloride

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: NSLS-II / Beamline: 19-ID / Wavelength: 0.9786 Å
DetectorType: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: Nov 4, 2024
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9786 Å / Relative weight: 1
ReflectionResolution: 2.05→47 Å / Num. obs: 57450 / % possible obs: 99.5 % / Redundancy: 4.1 % / Biso Wilson estimate: 27.63 Å2 / CC1/2: 0.988 / CC star: 0.997 / Rmerge(I) obs: 0.139 / Rpim(I) all: 0.074 / Rrim(I) all: 0.158 / Χ2: 1.056 / Net I/σ(I): 10.86
Reflection shellResolution: 2.05→2.09 Å / Redundancy: 2.4 % / Rmerge(I) obs: 0.736 / Mean I/σ(I) obs: 1.06 / Num. unique obs: 2747 / CC1/2: 0.484 / CC star: 0.808 / Rpim(I) all: 0.539 / Rrim(I) all: 0.918 / Χ2: 0.515 / % possible all: 95.9

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Processing

Software
NameVersionClassification
PHENIX1.21.2_5419refinement
HKL-3000data reduction
HKL-3000data scaling
HKL-3000phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.05→46.74 Å / SU ML: 0.2638 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 26.8785
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2507 2746 4.79 %
Rwork0.21 54596 -
obs0.212 57342 98.72 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 37.88 Å2
Refinement stepCycle: LAST / Resolution: 2.05→46.74 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6338 0 107 266 6711
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00246609
X-RAY DIFFRACTIONf_angle_d0.52278944
X-RAY DIFFRACTIONf_chiral_restr0.0406905
X-RAY DIFFRACTIONf_plane_restr0.00431181
X-RAY DIFFRACTIONf_dihedral_angle_d18.14192457
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.05-2.090.37931060.28312238X-RAY DIFFRACTION81.67
2.09-2.120.34251330.28532707X-RAY DIFFRACTION97.49
2.13-2.170.34391460.28472719X-RAY DIFFRACTION98.9
2.17-2.210.31111410.26162699X-RAY DIFFRACTION99.4
2.21-2.260.28131330.24392779X-RAY DIFFRACTION99.97
2.26-2.310.28841370.23862738X-RAY DIFFRACTION99.58
2.31-2.370.28471540.23412723X-RAY DIFFRACTION99.86
2.37-2.430.29241440.24272745X-RAY DIFFRACTION100
2.43-2.50.29521300.24392772X-RAY DIFFRACTION99.93
2.5-2.580.29531330.22892772X-RAY DIFFRACTION99.93
2.58-2.680.28981280.23482749X-RAY DIFFRACTION99.97
2.68-2.780.26311150.2312791X-RAY DIFFRACTION100
2.78-2.910.26271250.22272787X-RAY DIFFRACTION99.97
2.91-3.060.26051400.22962751X-RAY DIFFRACTION99.86
3.06-3.260.24971380.22792751X-RAY DIFFRACTION99.83
3.26-3.510.26171610.20372741X-RAY DIFFRACTION99.9
3.51-3.860.23991500.18862776X-RAY DIFFRACTION99.93
3.86-4.420.21061260.16372783X-RAY DIFFRACTION99.86
4.42-5.570.18911480.16142761X-RAY DIFFRACTION99.32
5.57-46.740.18711580.18022814X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.69781356902-0.2091560500970.5668742843692.146614298760.52918076451.298408966980.019745659370.3059635677350.254191887591-0.112675805221-0.04322974664370.0804646313645-0.07906978438880.07563793164140.02901960363880.1111086308260.0125440137919-0.01396272390860.2197250287890.01221122380980.14980166056911.444643679514.431946296819.2069808943
24.143370078811.2376690064-0.1855869111582.248025401930.2813135110981.31133531470.10442860554-0.434019234582-0.08848673915670.359819065027-0.1295685388590.03523102420740.09300547341420.01097624872440.02169893817510.1925924224180.019398599735-0.01464157374870.2026234514540.02250117418820.16269250113415.23399904488.4779077751332.4297720426
31.13672012532-0.874256931334-0.6821465768013.51391142620.8140480702522.37080420043-0.887280148080.0121382815416-0.8591189536591.05290768917-0.00171948086160.9499224962181.43050185861-0.1061356739920.4002827159830.7293551049740.003689930109380.2830352365020.204069319378-0.002862416480570.46449571585911.206291315-20.422660522810.3816612996
45.986423404741.528429204252.218847500485.593339678722.29830893077.563353259020.1183196455240.040732970246-0.0757888395819-0.2619084675630.0188226482332-0.0667150337361-0.031093960259-0.199907724662-0.1492433688960.1622804288990.05454359975190.03432171539960.1183583547170.01152536956420.11660400106317.1013130176-4.386754991764.40194131666
51.66317504544-0.504114842807-0.1188097403091.849151796242.601860106357.876370174950.1472468343580.2088758378080.259905633437-0.574875403490.105608179372-0.225712191332-0.6133611958190.250364831516-0.1513718981330.28423127368-0.001339256767560.07185122194040.1561022008540.006782784227050.24325661323119.37721979945.118414277451.96879378401
63.189776908070.5158180209551.279322291232.588317400263.025930700958.184067097370.4053561554980.366719620695-0.299169849581-0.4699606996910.0555214385074-0.1771365196020.3637180310370.0607911753276-0.4267775237620.3346681414980.0506387766474-0.0186396243960.142531627114-0.002958305677750.2677143156915.9596033588-1.79935844612.99687788567
73.389015536852.56700092192.118031735174.445703953193.490207773234.95178299305-0.159942905870.289104084963-0.423832805107-0.5087649685990.506293728097-0.664865495722-0.1831546212650.671994873635-0.3574845473380.2954658838170.01705349429870.08387320763280.284675241299-0.07406885453670.30330687641724.7749639872-4.18607680345-0.0327425517471
81.075645547061.655789936531.12127343892.637036128111.890922330011.358974068660.179695060089-0.1057565060990.6411347836050.477597813366-0.2976175771180.1553611607850.2228083644180.09432690684630.1348729635250.226235507964-0.04527728313510.002917168307310.2933332114840.005335812366010.24558294570913.29581323417.353633183630.1851047224
93.80875253682-1.046022998031.294817479512.333791930920.2471864638072.30148217605-0.042120538799-0.179209800812-0.2829476609210.07206545687510.092339849750.1679500284260.0682295203557-0.11703862382-0.07087015694170.245231301883-0.04423195475390.06377015431780.160905722989-0.03597342576510.1690353577254.55298317452-59.332755006733.1255770343
103.02695374-0.8959956784130.4811632023962.32663943791-0.5062505695772.86472610734-0.009845772201980.2686393614420.129962320413-0.4188547117470.0141998275769-0.431188301689-0.07055053446370.5345296757520.006341347841930.320091612131-0.070480915350.09535712071930.229095768078-0.05813901055090.29554836463117.2298818243-53.274747462527.9265521705
111.592724653890.635158298976-0.3361795481153.697044951370.8391440536781.95238539770.1373066708710.0870566261770.24571371224-0.5437810102340.0003821861279370.00195539111834-0.524322084795-0.058706990146-0.135407565290.4483261333290.0314721033910.07572274811530.1909528524920.02105551300560.24617374214-3.65066074996-24.542811075237.9184056094
121.81464508969-1.38281082105-0.1692202092769.06664460181-3.350588143182.00148772945-0.144259340866-0.416558076020.4176555161410.192176299641-0.394064787141-1.05033309155-0.08750781259190.7527999641350.476519752380.391957331163-0.06658929373670.003971263108490.467866641275-0.01710024839130.30316051458213.7855422659-45.94766249650.1324240828
139.13302423213-2.975521126581.463285780845.44864553149-1.525305790915.787467524360.1277513451750.06934623415730.475401325419-0.1432875395240.0514650311781-0.113989533522-0.5428698976510.0524043663876-0.1384795154040.254012077092-0.00409000876080.05445603130.1030416169080.0002307618376370.177219342589-3.3602468895-38.096971311447.2956733263
143.444506080750.913534170909-3.415365176670.419343385333-0.5487335802174.11402579302-0.07113973280131.193466189620.7916233862770.04727936570070.09470909645371.07292824373-0.873032136534-1.33430778597-0.04185773401550.396468589820.327419197906-0.03487589894050.8345323738560.1450090867630.5046109411-18.7573742071-35.675257153143.7785239357
156.29836754906-2.35550233873-2.353816012252.790380471290.588573030482.27914433253-0.0281711189568-0.224627617936-0.09330158019210.08505726288720.1300707717750.01621448091610.1833200207350.0677146391166-0.1075603200480.287068438-0.0275794686698-0.008859177971950.206830251855-0.04969790346090.157790395789-2.90706081831-45.845986888948.6992661603
167.64391370765-2.86494135777-7.724230931524.970959574092.917005646739.03486251356-0.697175331266-0.00753596251371-1.049329604170.781641751685-0.06924980887410.668138848871.41884749033-0.4258394523930.6747273095710.42270451855-0.05549773617190.03465720089930.2643672148040.02603254252780.309375761823-11.5396225201-50.369480255852.7911846369
172.68086923-0.69509012197-2.917288332231.060253785460.2568575977433.48221514939-0.0148990050695-0.06733230466390.4243752342530.1419829234880.20410143972-0.197226598155-0.0831882154370.21040966163-0.1510293581670.288724108842-0.01069028598570.0081185173460.184585938718-0.05459474375250.2384091182997.81966337658-47.478100033738.8807080351
182.28411658661-1.70055691436-1.78472287953.837976309660.3833136956333.57079794621-0.236999789578-0.1580963219090.4939562641930.6569882558360.2971455862340.392217027546-0.178623415932-0.5718775855970.02428807396040.299986843702-0.01125939643080.005721471326940.276886285819-0.02485286784810.329376181439-11.3899895056-42.869298771148.9272245833
197.44002187923-5.1763259534-2.204821945293.846554298741.311339114410.8792628345-0.452374377569-0.9405603737070.1125916814270.3706757809440.577143786415-0.0918921463570.03653568210960.299960763213-0.09013335676580.3302545022570.03359743662870.002959294375980.386178732621-0.005707413608070.2060734066250.876418494466-45.336213639357.0433010951
201.187971761631.576056191310.3850977819832.83351743941-1.578358869636.306347262050.0755657627371-0.03576221509910.5977169866590.7997140737450.1419570122470.320581424494-0.742495629377-0.0529394758237-0.3615469946510.5208172523820.03345433015280.1627047834540.345593171806-0.0641353689150.339624770364-7.80919262053-34.614744516353.1734961049
210.972371161866-1.33386405394-0.4431858326972.624813591250.3365191837810.3002252811150.3097952708380.3411230960330.0285427706393-0.0628199588527-0.201273006341-0.3256021558770.1651009982180.0631588725705-0.09571243665550.339018828648-0.04353449226690.02807037345130.282624759365-0.06276331945070.22136795474114.2867971169-62.221153135527.9473051324
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 84 )AA1 - 841 - 84
22chain 'A' and (resid 85 through 174 )AA85 - 17485 - 174
33chain 'A' and (resid 175 through 276 )AA175 - 276175 - 276
44chain 'B' and (resid 0 through 30 )BI0 - 301 - 31
55chain 'B' and (resid 31 through 56 )BI31 - 5632 - 57
66chain 'B' and (resid 57 through 77 )BI57 - 7758 - 78
77chain 'B' and (resid 78 through 99 )BI78 - 9979 - 100
88chain 'C' and (resid 1 through 9 )CL1 - 91 - 9
99chain 'D' and (resid 1 through 84 )DO1 - 841 - 84
1010chain 'D' and (resid 85 through 174 )DO85 - 17485 - 174
1111chain 'D' and (resid 175 through 276 )DO175 - 276175 - 276
1212chain 'E' and (resid 0 through 5 )EV0 - 51 - 6
1313chain 'E' and (resid 6 through 11 )EV6 - 117 - 12
1414chain 'E' and (resid 12 through 19 )EV12 - 1913 - 20
1515chain 'E' and (resid 20 through 41 )EV20 - 4121 - 42
1616chain 'E' and (resid 42 through 51 )EV42 - 5143 - 52
1717chain 'E' and (resid 52 through 61 )EV52 - 6153 - 62
1818chain 'E' and (resid 62 through 77 )EV62 - 7763 - 78
1919chain 'E' and (resid 78 through 90 )EV78 - 9079 - 91
2020chain 'E' and (resid 91 through 99 )EV91 - 9992 - 100
2121chain 'F' and (resid 1 through 9 )FZ1 - 91 - 9

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