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- PDB-9egd: AclA from Tenacibaculum discolor in complex with intermediate for... -

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Open data

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Basic information

Entry

Database: PDB / ID: 9egd

Title

AclA from Tenacibaculum discolor in complex with intermediate formed by C8-Lysine attack on ADP

Components

THIF-type NAD/FAD binding fold domain-containing protein

Keywords

TRANSFERASE / N-acyl-cyclolysine / biosynthesis / signaling

Function / homology

Function and homology information

tRNA threonylcarbamoyladenosine dehydratase / cyclic threonylcarbamoyladenosine biosynthetic process / ubiquitin-like modifier activating enzyme activity
Similarity search - Function

Biological species

Tenacibaculum discolor (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 3.15 Å

Authors

Shirkey, J.D. / Jeffrey, P.D. / Linares-Otoya, L. / Khatri Chhetri, B. / Donia, M.S. / Hughson, F.M.

Funding support

United States, 1items

Citation

Journal: To Be Published
Title: Discovery and characterization of a widespread chemical signaling pathway in environmental and host-associated Bacteroidetes
Authors: Linares-Otoya, L. / Shirkey, J.D. / Khatri Chhetri, B. / Mira, A. / Biswas, A. / Neff, S.L. / Linares-Otoya, M.V. / Chen, Y. / Campos-Florian, J.V. / Ganoza-Yupanqui, M.L. / Jeffrey, P.D. / ...

History

Deposition	Nov 21, 2024	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Aug 13, 2025	Provider: repository / Type: Initial release

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Structure visualization

Structure viewer	Molecule: MolmilJmol/JSmol

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Downloads & links

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Download

PDBx/mmCIF format	9egd.cif.gz	870.4 KB	Display	PDBx/mmCIF format
PDB format	pdb9egd.ent.gz		Display	PDB format
PDBx/mmJSON format	9egd.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	9egd_validation.pdf.gz	2.9 MB	Display	wwPDB validaton report
Full document	9egd_full_validation.pdf.gz	3 MB	Display
Data in XML	9egd_validation.xml.gz	73.7 KB	Display
Data in CIF	9egd_validation.cif.gz	90.4 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/eg/9egd ftp://data.pdbj.org/pub/pdb/validation_reports/eg/9egd	HTTPS FTP

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Related structure data

Related structure data	9egbC 9egcC C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#203 - Nov 2016 Aminopeptidase 1 and Autophagy similarity (3) #60 - Dec 2004 Ubiquitin similarity (3) #129 - Sep 2010 Isocitrate Dehydrogenase similarity (1) #154 - Oct 2012 Citric Acid Cycle similarity (1) #24 - Dec 2001 Glycogen Phosphorylase similarity (1) #55 - Jul 2004 DNA Ligase similarity (1) #16 - Apr 2001 Aminoacyl-tRNA Synthetases similarity (1)

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Assembly

Deposited unit

A: THIF-type NAD/FAD binding fold domain-containing protein

B: THIF-type NAD/FAD binding fold domain-containing protein

C: THIF-type NAD/FAD binding fold domain-containing protein

D: THIF-type NAD/FAD binding fold domain-containing protein

E: THIF-type NAD/FAD binding fold domain-containing protein

F: THIF-type NAD/FAD binding fold domain-containing protein

G: THIF-type NAD/FAD binding fold domain-containing protein

hetero molecules

215 kDa, 7 polymers
Search similar-shape structures of this assembly by Omokage search (details)

A: THIF-type NAD/FAD binding fold domain-containing protein

B: THIF-type NAD/FAD binding fold domain-containing protein

hetero molecules

defined by author&software
Evidence: gel filtration
61.6 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

C: THIF-type NAD/FAD binding fold domain-containing protein

D: THIF-type NAD/FAD binding fold domain-containing protein

hetero molecules

defined by author&software
Evidence: gel filtration
61.4 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

E: THIF-type NAD/FAD binding fold domain-containing protein

F: THIF-type NAD/FAD binding fold domain-containing protein

hetero molecules

defined by author&software
Evidence: gel filtration
61.4 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

G: THIF-type NAD/FAD binding fold domain-containing protein

hetero molecules

G: THIF-type NAD/FAD binding fold domain-containing protein

hetero molecules

defined by author&software
Evidence: gel filtration
61.6 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	204.122, 83.875, 162.948
Angle α, β, γ (deg.)	90.000, 105.491, 90.000
Int Tables number	5
Space group name H-M	C121
Space group name Hall	C2y

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID
1	1
2	1
3	1
4	1
5	1
6	1
7	1

NCS domain segments:

Ens-ID: 1

Dom-ID	Component-ID	Beg auth comp-ID	Beg label comp-ID	End auth comp-ID	End label comp-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	LYS	LYS	GLU	GLU	(chain 'A' and (resid 2 through 250 or resid 301 through 302))	A	A	2 - 249	16 - 263
1	2	AMP	AMP	A1BIH	A1BIH	(chain 'A' and (resid 2 through 250 or resid 301 through 302))	A	H - I	301 - 302
2	1	LYS	LYS	GLU	GLU	(chain 'B' and (resid 2 through 249 or (resid 250...	B	B	2 - 249	16 - 263
2	2	AMP	AMP	A1BIH	A1BIH	(chain 'B' and (resid 2 through 249 or (resid 250...	B	J - K	301 - 302
3	1	LYS	LYS	GLU	GLU	(chain 'C' and (resid 2 through 249 or (resid 250...	C	C	2 - 249	16 - 263
3	2	AMP	AMP	A1BIH	A1BIH	(chain 'C' and (resid 2 through 249 or (resid 250...	C	N - O	301 - 302
4	1	LYS	LYS	GLU	GLU	(chain 'D' and (resid 2 through 250 or resid 301 through 302))	D	D	2 - 249	16 - 263
4	2	AMP	AMP	A1BIH	A1BIH	(chain 'D' and (resid 2 through 250 or resid 301 through 302))	D	P - Q	301 - 302
5	1	LYS	LYS	GLU	GLU	(chain 'E' and (resid 2 through 249 or (resid 250...	E	E	2 - 249	16 - 263
5	2	AMP	AMP	A1BIH	A1BIH	(chain 'E' and (resid 2 through 249 or (resid 250...	E	R - S	301 - 302
6	1	LYS	LYS	GLU	GLU	(chain 'F' and (resid 2 through 249 or (resid 250...	F	F	2 - 249	16 - 263
6	2	AMP	AMP	A1BIH	A1BIH	(chain 'F' and (resid 2 through 249 or (resid 250...	F	T - U	301 - 302
7	1	LYS	LYS	GLU	GLU	(chain 'G' and (resid 2 through 249 or (resid 250...	G	G	2 - 249	16 - 263
7	2	AMP	AMP	A1BIH	A1BIH	(chain 'G' and (resid 2 through 249 or (resid 250...	G	V - W	301 - 302

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Components

#1: Protein	THIF-type NAD/FAD binding fold domain-containing protein Mass: 30056.287 Da / Num. of mol.: 7 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Tenacibaculum discolor (bacteria) / Gene: CSC81_02270 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A2G1BYE5
#2: Chemical	ChemComp-AMP / ADENOSINE MONOPHOSPHATE Mass: 347.221 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C₁₀H₁₄N₅O₇P / Comment: AMP*YM
#3: Chemical	ChemComp-A1BIH / N~2~-octanoyl-L-lysine Mass: 272.384 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C₁₄H₂₈N₂O₃ / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical	ChemComp-PEG / DI(HYDROXYETHYL)ETHER Mass: 106.120 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C₄H₁₀O₃
Has ligand of interest	Y
Has protein modification	N

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Experimental details

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.2 Å³/Da / Density % sol: 61.51 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7 / Details: pH 7.0, DL-Malic Acid, PEG-3350, MgCl2

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: NSLS-II / Beamline: 17-ID-1 / Wavelength: 0.9201 Å
Detector	Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Jul 28, 2023
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.9201 Å / Relative weight: 1
Reflection	Resolution: 3.15→29.62 Å / Num. obs: 45960 / % possible obs: 99 % / Redundancy: 3.9 % / CC_1/2: 0.987 / R_merge(I) obs: 0.231 / R_pim(I) all: 0.134 / R_rim(I) all: 0.268 / Χ²: 0.98 / Net I/σ(I): 5.3
Reflection shell	Resolution: 3.15→3.26 Å / Redundancy: 4 % / R_merge(I) obs: 1.536 / Num. unique obs: 4488 / CC_1/2: 0.541 / R_pim(I) all: 0.867 / R_rim(I) all: 1.767 / Χ²: 0.9

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Processing

Software

Name	Version	Classification
PHENIX	1.17_3644	refinement
Aimless		data scaling
XDS		data reduction
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 3.15→29.62 Å / SU ML: 0.4709 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 28.0731
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2494	2354	5.13 %
R_work	0.1935	43551	-
obs	0.1964	45905	99.3 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 88.97 Å²

Refinement step

Cycle: LAST / Resolution: 3.15→29.62 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	13987	0	308	0	14295

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0082	14606
X-RAY DIFFRACTION	f_angle_d	1.2727	19699
X-RAY DIFFRACTION	f_chiral_restr	0.0724	2145
X-RAY DIFFRACTION	f_plane_restr	0.0077	2493
X-RAY DIFFRACTION	f_dihedral_angle_d	23.2938	5500

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
3.15-3.21	0.4154	128	0.368	2522	X-RAY DIFFRACTION	98.88
3.21-3.28	0.3457	131	0.3164	2553	X-RAY DIFFRACTION	99.44
3.28-3.36	0.3753	136	0.3044	2551	X-RAY DIFFRACTION	99.04
3.36-3.44	0.3692	145	0.283	2547	X-RAY DIFFRACTION	99.3
3.44-3.54	0.3262	137	0.2843	2529	X-RAY DIFFRACTION	99.22
3.54-3.64	0.2983	148	0.2556	2530	X-RAY DIFFRACTION	99.33
3.64-3.76	0.319	131	0.2206	2540	X-RAY DIFFRACTION	99.11
3.76-3.89	0.2665	135	0.2187	2548	X-RAY DIFFRACTION	99.22
3.89-4.05	0.275	132	0.2042	2550	X-RAY DIFFRACTION	99.3
4.05-4.23	0.243	135	0.1762	2586	X-RAY DIFFRACTION	99.52
4.23-4.45	0.2277	150	0.1515	2541	X-RAY DIFFRACTION	99.34
4.45-4.73	0.1807	143	0.142	2533	X-RAY DIFFRACTION	98.75
4.73-5.1	0.1967	143	0.1542	2572	X-RAY DIFFRACTION	99.23
5.1-5.61	0.2503	145	0.1648	2579	X-RAY DIFFRACTION	99.49
5.61-6.41	0.2082	128	0.169	2602	X-RAY DIFFRACTION	99.71
6.41-8.05	0.2113	149	0.1677	2599	X-RAY DIFFRACTION	99.82
8.05-29.62	0.1901	138	0.1469	2669	X-RAY DIFFRACTION	99.4

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.366987955019	1.08281872042	-1.47172458314	9.25715932453	-7.10994128041	7.18691783873	-0.188097927229	-0.127021330405	1.31027269416	1.78577935568	-0.410448722795	0.205989924601	-0.670913027666	-0.968310560518	0.733753741434	0.739718649302	0.237154163297	-0.0701047083343	0.608771500005	-0.226538713987	1.21069972832	-59.331397734	18.0728895082	62.822530767
2	3.27615273876	1.5350427809	1.16618273325	3.54775189555	1.00110000236	4.70558819015	-0.308067872169	-0.130279108779	1.34477416809	0.302968918059	0.055117309656	-0.0596773530526	-0.457956845421	0.0270772377475	0.160165866013	0.811243958195	0.0127513740632	-0.361133712066	0.335416254005	-0.0489824297306	1.06449283184	-41.7063411906	23.054711011	61.6902190021
3	0.333065791092	0.403740999109	0.263214951401	1.64220175648	-0.0447602744924	0.684640193736	-0.459134406625	-0.128204821098	0.591332589507	0.218298375242	-0.248015787844	-0.0241691699749	-0.660018964186	-0.178523485437	-0.113459429984	1.10332990016	-0.0268634034417	-0.633897820285	0.314533831118	-0.0685510827346	1.41946248022	-36.9842061029	30.6814418523	59.4152460004
4	0.00501776808581	0.070741038683	0.0291886652521	1.12675858932	0.502546079914	0.220575951667	0.606392738787	-0.705742024285	0.63627778234	0.408171988451	-0.182853931047	0.0166355770815	-0.499141384547	0.332445876183	0.931879426879	0.953242811429	-0.288914525507	-0.48943442621	0.382117642815	-0.371366660233	0.853023986103	-32.699294331	21.5663760743	72.3498313156
5	0.976632613942	-0.336267061233	0.362890111555	3.04993377378	1.21409493825	2.34143155174	0.144369588243	-0.364848700496	0.0717054417586	0.621646543463	0.103773590884	-0.809501029099	-0.118627222191	0.121461389296	0.20382312188	0.79526221002	-0.0323341877852	-0.434224003265	0.154282672055	-0.0582950707204	0.978586520981	-29.527830063	13.9830105191	63.6945156155
6	2.72096055631	-0.161910212843	1.23280964976	6.65357406534	2.43931112873	2.60549790905	-0.112063777407	0.145099994166	0.0230825497811	-0.0101107555993	-0.19701190068	-1.03731222856	0.288092888417	0.847292617947	-0.087826514666	0.693419224534	0.0221048933232	-0.242170490743	0.472340143628	0.0269505377893	0.921167018898	-24.1133886441	4.78585603976	59.5801562128
7	3.17145892745	-1.62680743363	-1.52886801916	3.50493294401	-0.413111972158	6.55623405338	0.263984147869	0.303246067694	0.502326186338	-0.136278659544	-0.133841020058	-0.406324303524	0.301246396496	0.672939637929	-0.19413566624	0.611016321449	-0.0700728814534	-0.444467903628	0.514969289198	-0.0531869489014	0.902763472261	-32.4108057698	9.59373703386	50.4025912192
8	5.08762135532	-4.91460226133	0.306583547515	7.50380960201	-2.62722399352	6.76683976036	0.095430884586	-0.173172474267	-0.188699167692	0.520569110557	-0.137919310375	0.0330201262759	0.823445321066	-0.493231536323	-0.110318702356	0.653994706343	-0.124491765801	-0.356248105166	0.289407940397	0.0146838814883	0.687727718903	-39.2704269995	3.75085420226	60.4509786692
9	2.58515673026	-4.81096486535	-4.03986187094	9.39117446539	8.23679937318	7.45122402508	-1.01201237716	-0.0611596511697	0.760300251527	-1.57704880692	0.0685097570822	-0.358324886713	-2.28359111502	1.58680655772	0.475292673357	0.871102549423	-0.330207981384	-0.537316656482	0.956452130561	0.246296240983	1.54152042316	-30.4848299688	20.2576402423	39.2953563895
10	1.97922158654	-0.997316158543	0.481745832807	3.24768632512	0.632027666043	3.12163298407	-0.628098611776	0.328020878877	0.994052015569	-0.575039662596	-0.221238349605	0.468254030492	-0.946129422441	-0.209195951069	0.532994517977	0.862866567494	0.00746177178788	-0.484262658121	0.545094311495	0.0663844561172	1.21087772785	-51.9935368274	24.7829908141	40.262745833
11	2.42478890706	0.110837908004	-0.426966696009	2.24022090065	-2.13632883605	7.61348410309	-0.0475299158576	0.661472932808	0.109475343891	-0.102705292906	0.0771209694226	0.601902713135	-0.386177660945	-0.216320277837	-0.0747659358205	0.744865654616	0.0653426085189	-0.486295405164	0.472033662016	0.0771011347854	1.09020976158	-57.9586055648	11.8468754038	37.3245115246
12	1.59782776429	-0.142439801977	1.75086906774	2.29233196878	0.500519867091	7.25180177238	-0.193231171366	0.126269604163	-0.283450439772	-0.219589741557	-0.432511613383	0.690145226421	-0.403818499651	-0.699242290207	-0.025329675837	0.663962290269	0.0675459484685	-0.278015012152	0.557451942935	-0.212449347315	1.01776641519	-61.95455589	0.222305344892	43.2031154411
13	1.35420773816	-1.91766951887	-0.27873740879	5.07332341593	3.53789420108	7.87548653821	-0.194811842694	-0.0339525728614	-0.500845951758	0.993934234266	-0.238380480524	1.29644977543	0.535748757195	-0.839706093173	0.703942160411	0.685175201327	-0.118822592625	-0.176005583785	0.522098847849	-0.0857057699323	1.04385411796	-62.1171262657	5.29399533876	58.2817810902
14	5.26072624399	2.62986192633	-3.07408742207	1.33372931025	-1.62531072586	9.26868694424	-0.0524072967412	0.349747433509	-0.07272085679	0.0039244338329	-0.134045284375	-0.343483784821	-0.342617080465	0.151311202636	0.345080505575	0.504285640254	0.0225347430758	-0.191387021054	0.394971131902	0.0577314908866	0.922585738744	-45.553936112	12.6122874179	43.8266941965
15	8.22400149495	4.46053909832	-1.19861228227	4.03720352185	2.06688939087	5.57074299374	-0.138076310686	0.654074631307	-0.89270147068	-0.179813267425	-0.0786293441272	-0.350455840824	1.14594001276	0.0581711479946	0.188898468772	0.591189735236	0.0901293866165	-0.336284165088	0.376706381678	-0.112047109796	1.0103454192	-47.1046741476	2.81327175895	41.9608990459
16	9.78517105988	0.785795674639	0.177489377077	3.25614805695	-4.67768245648	6.91489252308	-0.405091072364	-0.502493067118	1.65813834639	0.840407696685	0.297083261341	-0.752676965147	-1.26044134546	-0.18246500449	-0.0482718766574	0.693132188763	0.126789849229	-0.256948865544	0.690177657395	-0.197867596764	1.4259058357	-4.84424901605	59.0180116119	24.8086760196
17	4.28720089206	-1.22139324579	-0.935132135293	6.22254349329	-0.620112302501	2.87596427671	0.0352359964847	-0.253762636815	0.784860735985	-0.252142756362	0.297548059657	0.833850153086	-0.255490004065	-1.00609707915	0.0167083907565	0.302580323588	0.104010768872	0.0581813637259	0.988007490244	-0.0816786557201	0.551741290319	-15.7363386813	46.6305604384	25.9224235403
18	2.79355584217	-1.94575282136	-1.59444196005	3.5127604567	1.12961080828	2.37563297866	-0.192576307922	-0.493447274362	0.449724867516	0.152150669131	0.13809207719	1.16979159992	-0.586428637535	-0.786718006295	-0.145824815054	0.328222257301	0.138758842098	0.0488822481408	1.10762384613	-0.129454215362	0.780775436001	-19.4011749573	47.2447816909	31.0578489982
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55	5.58068949165	3.20681724418	0.437398735597	2.46130839697	1.13795108618	1.31589663548	0.188887102124	0.451727104936	-0.477355138481	-0.579306931979	-0.232158232703	0.274940509148	0.0830893927419	-0.384105355331	0.0191983073228	0.779814389379	-0.0210494685271	-0.487025995518	0.230613181301	0.0395717210507	0.966380474019	-33.4911201789	58.2950286733	62.874687998
56	5.54408909246	2.1590394503	2.04720286021	3.48913487025	0.637526397974	2.08100075332	0.429740206224	0.949369120235	0.113784683926	-1.21999959745	-0.0174122797842	0.676696571875	-0.151610761818	0.306040111491	-0.40263908177	1.31293979865	0.135884933954	-0.686853205198	0.629408205959	-0.0680321283245	1.24135229699	-31.7636659956	66.9559772978	54.8429001163
57	5.20476779651	-3.47719346596	2.37973941048	5.68074364952	0.646574009676	2.66365221343	-0.0975700277627	0.850791221271	0.428957173698	-0.310904582252	-0.0911690137847	-0.375795077725	-0.185548902133	0.475876278792	-0.0791057966414	0.685459663554	0.0107405464155	-0.206939866057	0.374740408316	0.00366067224664	0.972429765412	-15.9285572585	68.0670922982	62.2933175348
58	3.63410304	-1.00686121474	-0.0568120102049	0.6257521912	-1.1552326628	4.13505257835	0.294178620659	-0.179570623582	0.0642681452846	-0.0774663448426	-0.461674729462	0.285312088621	-0.439187261611	-0.184565198498	0.377682812348	0.734761197892	-0.0172062613817	-0.378275684275	0.361811685836	0.0521547105961	0.956875807786	-29.6817884439	63.7896398465	74.7170125349

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 1 through 13 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 14 through 62 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 63 through 103 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 104 through 116 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 117 through 154 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 155 through 186 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 187 through 233 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 234 through 250 )
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 1 through 13 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 14 through 108 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 109 through 154 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 155 through 187 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 188 through 213 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 214 through 232 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 233 through 250 )
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 2 through 31 )
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 32 through 60 )
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 61 through 95 )
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 96 through 123 )
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 124 through 154 )
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 155 through 172 )
22	X-RAY DIFFRACTION	22	chain 'C' and (resid 173 through 213 )
23	X-RAY DIFFRACTION	23	chain 'C' and (resid 214 through 250 )
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 1 through 13 )
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 14 through 74 )
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 75 through 95 )
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 96 through 108 )
28	X-RAY DIFFRACTION	28	chain 'D' and (resid 109 through 123 )
29	X-RAY DIFFRACTION	29	chain 'D' and (resid 124 through 135 )
30	X-RAY DIFFRACTION	30	chain 'D' and (resid 136 through 154 )
31	X-RAY DIFFRACTION	31	chain 'D' and (resid 155 through 172 )
32	X-RAY DIFFRACTION	32	chain 'D' and (resid 173 through 187 )
33	X-RAY DIFFRACTION	33	chain 'D' and (resid 188 through 232 )
34	X-RAY DIFFRACTION	34	chain 'D' and (resid 233 through 250 )
35	X-RAY DIFFRACTION	35	chain 'E' and (resid 2 through 31 )
36	X-RAY DIFFRACTION	36	chain 'E' and (resid 32 through 74 )
37	X-RAY DIFFRACTION	37	chain 'E' and (resid 75 through 95 )
38	X-RAY DIFFRACTION	38	chain 'E' and (resid 96 through 108 )
39	X-RAY DIFFRACTION	39	chain 'E' and (resid 109 through 123 )
40	X-RAY DIFFRACTION	40	chain 'E' and (resid 124 through 154 )
41	X-RAY DIFFRACTION	41	chain 'E' and (resid 155 through 172 )
42	X-RAY DIFFRACTION	42	chain 'E' and (resid 173 through 213 )
43	X-RAY DIFFRACTION	43	chain 'E' and (resid 214 through 233 )
44	X-RAY DIFFRACTION	44	chain 'E' and (resid 234 through 250 )
45	X-RAY DIFFRACTION	45	chain 'F' and (resid 2 through 62 )
46	X-RAY DIFFRACTION	46	chain 'F' and (resid 63 through 74 )
47	X-RAY DIFFRACTION	47	chain 'F' and (resid 75 through 95 )
48	X-RAY DIFFRACTION	48	chain 'F' and (resid 96 through 108 )
49	X-RAY DIFFRACTION	49	chain 'F' and (resid 109 through 154 )
50	X-RAY DIFFRACTION	50	chain 'F' and (resid 155 through 172 )
51	X-RAY DIFFRACTION	51	chain 'F' and (resid 173 through 232 )
52	X-RAY DIFFRACTION	52	chain 'F' and (resid 233 through 250 )
53	X-RAY DIFFRACTION	53	chain 'G' and (resid 2 through 31 )
54	X-RAY DIFFRACTION	54	chain 'G' and (resid 32 through 108 )
55	X-RAY DIFFRACTION	55	chain 'G' and (resid 109 through 154 )
56	X-RAY DIFFRACTION	56	chain 'G' and (resid 155 through 172 )
57	X-RAY DIFFRACTION	57	chain 'G' and (resid 173 through 213 )
58	X-RAY DIFFRACTION	58	chain 'G' and (resid 214 through 250 )

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