PDB-9d7d: OXA-58-NA-1-157 20 min complex

ID or keywords:

Entry

Database: PDB / ID: 9d7d

Title

OXA-58-NA-1-157 20 min complex

Components

Beta-lactamase

Keywords

HYDROLASE/INHIBITOR / beta-lactamase / carbapenemase / antibiotic resistance / inhibitor / HYDROLASE / HYDROLASE-INHIBITOR complex

Function / homology

Function and homology information

penicillin binding / antibiotic catabolic process / cell wall organization / beta-lactamase / beta-lactamase activity / response to antibiotic / plasma membrane
Similarity search - Function

Biological species

Acinetobacter baumannii (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

FOURIER SYNTHESIS / Resolution: 2.05 Å

Authors

Smith, C.A. / Maggiolo, A.O. / Toth, M. / Vakulenko, S.B.

Funding support

United States, 1items

Citation

Journal: Acs Infect Dis. / Year: 2024
Title: Decarboxylation of the Catalytic Lysine Residue by the C5 alpha-Methyl-Substituted Carbapenem NA-1-157 Leads to Potent Inhibition of the OXA-58 Carbapenemase.
Authors: Toth, M. / Stewart, N.K. / Maggiolo, A.O. / Quan, P. / Khan, M.M.K. / Buynak, J.D. / Smith, C.A. / Vakulenko, S.B.

History

Deposition	Aug 16, 2024	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Dec 11, 2024	Provider: repository / Type: Initial release
Revision 1.1	Dec 25, 2024	Group: Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

Structure viewer	Molecule: MolmilJmol/JSmol

-
Download

PDBx/mmCIF format	9d7d.cif.gz	397.7 KB	Display	PDBx/mmCIF format
PDB format	pdb9d7d.ent.gz	327.6 KB	Display	PDB format
PDBx/mmJSON format	9d7d.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

-
Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/d7/9d7d ftp://data.pdbj.org/pub/pdb/validation_reports/d7/9d7d	HTTPS FTP

-
Related structure data

Related structure data	9d78C 9d79C 9d7aC 9d7bC 9d7cC 9d8cC C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

-
Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#263 - Nov 2021 Acetohydroxyacid Synthase similarity (1) #261 - Sep 2021 DNA-Sequencing Nanopores similarity (1) #22 - Oct 2001 Photosystem I similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (1) #29 - May 2002 Penicillin-binding Proteins similarity (1) #95 - Nov 2007 Multidrug Resistance Transporters similarity (1)

Deposited unit

A: Beta-lactamase

B: Beta-lactamase

C: Beta-lactamase

D: Beta-lactamase

hetero molecules

128 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Beta-lactamase

hetero molecules

defined by author&software
31.9 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Beta-lactamase

hetero molecules

defined by author&software
31.9 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

C: Beta-lactamase

hetero molecules

defined by author&software
31.9 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

D: Beta-lactamase

hetero molecules

defined by author&software
31.9 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	36.930, 66.584, 193.460
Angle α, β, γ (deg.)	90.00, 91.32, 90.00
Int Tables number	4
Space group name H-M	P12₁1

#1: Protein	Beta-lactamase Mass: 31455.205 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acinetobacter baumannii (bacteria) Gene: blaOXA-58, bla-oxa-58, bla-oxa58, GSE42_20550, P9867_20895 Production host: Escherichia coli (E. coli) / References: UniProt: Q2TR58, beta-lactamase
#2: Chemical	ChemComp-Y33 / (5R)-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-5-[(2S,3R)-3-hydroxy-1-oxobutan-2-yl]-5-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid Mass: 401.478 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₁₇H₂₇N₃O₆S / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical	ChemComp-CO2 / CARBON DIOXIDE Mass: 44.010 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: CO₂ / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical	ChemComp-ACT / ACETATE ION Mass: 59.044 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₂H₃O₂
#5: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 179 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	Y
Has protein modification	Y

-
Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

-
Sample preparation

Crystal	Density Matthews: 2.12 Å³/Da / Density % sol: 41.97 %
Crystal grow	Temperature: 289 K / Method: vapor diffusion, sitting drop Details: 0.2 M sodium acetate, 0.1 M Tris-HCl pH 8.5, 30% PEG4000

-
Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 0.9795 Å
Detector	Type: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: May 16, 2024
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.9795 Å / Relative weight: 1
Reflection	Resolution: 2.05→39.12 Å / Num. obs: 56857 / % possible obs: 96.4 % / Redundancy: 7.3 % / CC_1/2: 0.997 / R_pim(I) all: 0.058 / R_rim(I) all: 0.156 / Net I/σ(I): 8.8
Reflection shell	Resolution: 2.05→2.11 Å / Mean I/σ(I) obs: 1.4 / Num. unique obs: 4358 / CC_1/2: 0.585 / R_pim(I) all: 0.594

-
Processing

Software

Name	Version	Classification
PHENIX	(1.21.1_5286: ???)	refinement
XDS		data reduction
Aimless		data scaling
MOLREP		phasing

Refinement

Method to determine structure:

FOURIER SYNTHESIS / Resolution: 2.05→39.12 Å / SU ML: 0.26 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 24.08 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2328	2758	4.86 %
R_work	0.1838	-	-
obs	0.1862	56782	96.08 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 2.05→39.12 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	7874	0	4	179	8057

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.007	8055
X-RAY DIFFRACTION	f_angle_d	1.013	10907
X-RAY DIFFRACTION	f_dihedral_angle_d	18.62	2990
X-RAY DIFFRACTION	f_chiral_restr	0.055	1178
X-RAY DIFFRACTION	f_plane_restr	0.007	1409

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.05-2.09	0.319	145	0.2831	2599	X-RAY DIFFRACTION	95
2.09-2.12	0.339	135	0.2647	2690	X-RAY DIFFRACTION	97
2.12-2.16	0.2928	160	0.241	2742	X-RAY DIFFRACTION	97
2.16-2.21	0.2857	132	0.2388	2674	X-RAY DIFFRACTION	97
2.21-2.26	0.2936	129	0.2307	2685	X-RAY DIFFRACTION	96
2.26-2.31	0.2467	143	0.2267	2660	X-RAY DIFFRACTION	92
2.31-2.37	0.2618	116	0.2219	2455	X-RAY DIFFRACTION	90
2.37-2.43	0.2957	121	0.2215	2735	X-RAY DIFFRACTION	98
2.43-2.5	0.2713	141	0.2173	2735	X-RAY DIFFRACTION	98
2.5-2.58	0.2896	124	0.2201	2763	X-RAY DIFFRACTION	98
2.58-2.67	0.2976	148	0.2155	2750	X-RAY DIFFRACTION	98
2.68-2.78	0.2627	135	0.2035	2761	X-RAY DIFFRACTION	98
2.78-2.91	0.2669	130	0.2011	2748	X-RAY DIFFRACTION	98
2.91-3.06	0.2347	146	0.209	2762	X-RAY DIFFRACTION	97
3.06-3.25	0.2745	130	0.2064	2642	X-RAY DIFFRACTION	95
3.25-3.5	0.2205	145	0.1757	2556	X-RAY DIFFRACTION	90
3.51-3.86	0.2067	156	0.1633	2774	X-RAY DIFFRACTION	99
3.86-4.41	0.1719	132	0.1378	2800	X-RAY DIFFRACTION	99
4.42-5.56	0.1745	153	0.1359	2767	X-RAY DIFFRACTION	97
5.56-39.12	0.2122	137	0.1506	2726	X-RAY DIFFRACTION	93

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.2643	0.1253	-0.0749	0.1434	-0.1556	0.1902	0.1617	-0.7218	0.0795	0.652	0.1813	-0.432	0.0563	0.5083	0.0016	0.5643	-0.088	0.0043	0.8244	0.1227	0.5343	-14.5748	-6.2438	86.5032
2	1.4229	0.5161	0.3341	0.6706	0.5101	1.403	0.0567	-0.2521	0.0965	0.0116	-0.0529	-0.2077	-0.1122	0.3111	0.0001	0.3082	-0.0314	0.0086	0.3941	0.0155	0.2905	-18.6236	1.3455	69.6288
3	2.7197	0.0822	0.4986	0.9701	0.7867	1.9001	0.0721	0.2893	-0.3576	0.1412	-0.015	-0.0775	-0.0244	-0.0731	-0.0001	0.3282	0.0259	-0.0166	0.4009	-0.08	0.3148	-30.4152	-5.1442	51.8094
4	2.4073	-0.3664	1.201	1.236	-0.6387	3.1994	0.0968	-0.0875	-0.2381	-0.054	-0.0535	0.0452	-0.1822	0.0142	0.0001	0.3282	-0.0072	0.0044	0.3065	-0.0219	0.2791	-25.6074	0.503	64.461
5	0.7322	0.7433	0.621	1.5975	-0.1236	1.1684	-0.2445	-0.7274	-0.4928	0.4324	0.097	0.1268	0.2465	0.1875	-0.0004	0.353	0.0028	0.0377	0.5577	0.0418	0.3073	-23.7795	-1.2781	74.8992
6	0.4557	-0.0216	0.1718	0.4928	-0.4955	0.5337	0.0771	-0.5282	-0.7863	0.1809	-0.0928	0.0616	0.545	0.0924	-0.0009	0.4156	-0.0204	-0.0313	0.5317	0.1063	0.472	-22.3801	-10.7049	77.5319
7	1.708	0.3158	0.2025	1.2767	0.252	1.3478	0.0653	0.118	0.21	-0.1023	-0.0812	0.2727	-0.0501	-0.2739	0.0002	0.2721	0.0261	0.0339	0.208	0.0005	0.306	-19.3405	-28.9551	19.894
8	1.8361	-0.1313	0.6269	1.7273	-0.6982	2.2041	0.0657	-0.3496	-0.1046	0.0271	-0.0296	-0.158	0.0978	0.1317	-0.0001	0.2977	-0.0317	0.0383	0.2916	-0.0197	0.3076	-6.4267	-31.8451	41.9871
9	1.7715	0.3536	-0.0656	1.0333	0.7255	2.64	0.02	0.0169	-0.0314	-0.0018	0.0142	-0.0115	-0.1062	0.0066	0.0001	0.274	0.0111	0.0077	0.1802	0.0037	0.2943	-11.3949	-27.3529	28.8208
10	1.5082	-0.7774	0.3963	1.2703	0.4568	1.9737	0.0544	0.1854	-0.1709	-0.1353	-0.0552	0.0093	0.1582	0.1952	-0.0002	0.2536	-0.0198	-0.006	0.2587	-0.0156	0.3404	-14.0986	-36.2411	17.2937
11	0.0894	0.0647	-0.004	0.1384	-0.0562	0.309	-0.048	-0.8294	-0.1017	-0.1677	0.3338	0.8798	0.4151	-1.2345	-0.0017	0.4426	0.0221	-0.0454	0.6019	-0.0299	0.3875	-6.5623	5.3829	40.1431
12	0.8691	0.5938	-0.1946	0.5054	-0.525	1.3848	-0.0377	-0.003	-0.1088	-0.0237	-0.0051	0.0836	0.0217	-0.1156	0.0002	0.2672	0.0009	-0.0225	0.1931	-0.003	0.2719	-2.3143	-0.7058	22.5399
13	2.5527	-0.4271	0.2901	1.2851	-0.3961	0.6564	-0.2009	0.312	0.3125	-0.2075	0.1105	-0.0294	-0.1053	0.1288	-0.0002	0.3665	-0.0231	-0.0282	0.3293	0.0337	0.2623	9.3671	7.1136	5.2085
14	1.8017	-0.5025	-0.6306	0.6788	0.4145	1.396	-0.0034	0.0264	0.0943	-0.058	-0.0161	0.0756	0.0705	-0.0016	-0.0001	0.2927	-0.0106	-0.0183	0.174	0.0201	0.2639	4.6328	0.5732	17.5098
15	0.965	1.0148	-0.9329	1.3189	-0.7444	1.0945	-0.2411	-0.4684	0.2253	0.3489	0.1388	-0.2036	-0.0471	-0.0788	-0.0001	0.253	-0.0122	-0.059	0.2471	-0.0671	0.2727	2.8668	1.6127	27.971
16	0.8053	0.7643	-0.1837	0.8985	0.1376	0.5784	-0.1626	-0.3063	0.4141	0.2309	0.0498	0.1749	-0.2698	-0.1854	-0.0004	0.3108	0.03	-0.0104	0.2463	-0.0615	0.3245	1.3261	10.6231	31.5697
17	1.2814	-1.4594	-0.9687	1.7343	1.1829	0.8342	0.5101	0.5594	0.6418	-0.7606	0.0104	-0.5511	-0.0545	0.4536	0.1407	0.599	0.1617	0.0715	0.6408	0.183	0.4524	1.3906	26.4019	58.3952
18	1.1587	-0.0379	0.1812	1.0223	0.7754	1.5971	-0.068	0.0427	0.0967	0.1524	-0.0183	-0.2408	0.371	0.3045	-0.0001	0.3293	0.01	0.0213	0.4935	-0.0136	0.3317	-2.0259	24.8224	77.2656
19	1.2063	-0.0434	0.4055	0.2209	0.1187	0.6878	-0.1931	-0.3351	0.9586	-0.0325	-0.2477	-0.1664	-0.4051	-0.3963	-0.0303	0.5427	0.0188	-0.0178	0.7512	-0.1876	0.6752	-16.0238	40.2336	93.8257
20	1.0745	0.6502	0.3031	0.8731	0.8773	1.1473	-0.2851	-0.4458	0.1235	0.1088	0.1125	0.0056	0.1537	-0.3385	0.0001	0.4552	0.0345	0.0496	0.4948	-0.1053	0.3498	-12.4307	31.7612	92.9531
21	0.2726	0.0351	-0.2706	0.2296	-0.1796	0.3489	-0.1605	-0.3496	0.1812	0.2531	0.0388	-0.0616	0.3182	0.7225	-0.0001	0.4456	0.0564	0.0012	0.5483	-0.0693	0.3651	-0.781	27.0759	92.5834
22	1.514	-0.0443	0.2459	1.2282	-0.2659	1.7132	0.0591	0.2901	0.342	0.1278	-0.116	0.2522	0.1198	-0.4444	0.0003	0.3575	-0.0581	0.0196	0.4956	-0.0513	0.4178	-14.8878	25.2306	78.0776
23	0.4907	-0.2594	-0.3578	0.4684	-0.2447	0.8297	-0.0723	-0.0986	0.6417	-0.206	-0.1007	-0.3054	-0.2351	-0.0601	-0.0008	0.3157	0.0216	0.0279	0.5055	-0.0773	0.4758	-2.4177	31.8353	78.8613
24	0.7182	-0.5828	-0.5218	1.0152	-0.3152	1.3643	-0.0926	0.4055	0.3239	-0.5426	0.1275	0.1399	-0.1734	0.0981	-0.0004	0.4262	-0.0376	-0.0116	0.4765	0.0365	0.5058	-4.3994	31.4003	72.5122
25	0.2265	-0.0565	-0.188	0.1101	0.234	0.4929	0.2717	0.6188	0.5714	-0.7323	-0.1093	0.0212	-0.6518	-1.3722	0.0051	0.3955	0.0359	0.0183	0.701	0.1384	0.4601	-8.1151	32.3382	64.222

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 38 through 50 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 51 through 95 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 96 through 154 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 155 through 239 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 240 through 254 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 255 through 280 )
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 38 through 95 )
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 96 through 154 )
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 155 through 239 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 240 through 280 )
11	X-RAY DIFFRACTION	11	chain 'C' and (resid 38 through 50 )
12	X-RAY DIFFRACTION	12	chain 'C' and (resid 51 through 95 )
13	X-RAY DIFFRACTION	13	chain 'C' and (resid 96 through 154 )
14	X-RAY DIFFRACTION	14	chain 'C' and (resid 155 through 239 )
15	X-RAY DIFFRACTION	15	chain 'C' and (resid 240 through 254 )
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 255 through 280 )
17	X-RAY DIFFRACTION	17	chain 'D' and (resid 36 through 50 )
18	X-RAY DIFFRACTION	18	chain 'D' and (resid 51 through 95 )
19	X-RAY DIFFRACTION	19	chain 'D' and (resid 96 through 117 )
20	X-RAY DIFFRACTION	20	chain 'D' and (resid 118 through 154 )
21	X-RAY DIFFRACTION	21	chain 'D' and (resid 155 through 177 )
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 178 through 224 )
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 225 through 239 )
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 240 through 261 )
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 262 through 280 )

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

Version 3 of the EMDB header file is now the official format.
The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+
Jul 12, 2017. Major update of PDB

-
Yorodumi

Thousand views of thousand structures

Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

-
Movie

-
Structure viewers

+
Viewer log

-
This page

-
This web site

-
Options

+
Open data

-
Basic information

-
Structure visualization

-
Downloads & links

-
Download

-
Validation report

-
Related structure data

-
Links

-
Assembly

-
Components

-
Experimental details

-
Experiment

-
Sample preparation

-
Data collection

-
Processing

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

-
Aug 12, 2020. Covid-19 info

+
Mar 5, 2020. Novel coronavirus structure data

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+
Jul 12, 2017. Major update of PDB

-
Yorodumi

-Movie

-Structure viewers

+Viewer log

-This page

-This web site

-Options

+Open data

-Basic information

-Structure visualization

-Downloads & links

-Download

-Validation report

-Related structure data

-Links

-Assembly

-Components

-Experimental details

-Experiment

-Sample preparation

-Data collection

-Processing

+About Yorodumi

-News

-Feb 9, 2022. New format data for meta-information of EMDB entries

-Aug 12, 2020. Covid-19 info

+Mar 5, 2020. Novel coronavirus structure data

+Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+Jul 12, 2017. Major update of PDB

-Yorodumi

-
Movie

-
Structure viewers

+
Viewer log

-
This page

-
This web site

-
Options

+
Open data

-
Basic information

-
Structure visualization

-
Downloads & links

-
Download

-
Validation report

-
Related structure data

-
Links

-
Assembly

-
Components

-
Experimental details

-
Experiment

-
Sample preparation

-
Data collection

-
Processing

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

-
Aug 12, 2020. Covid-19 info

+
Mar 5, 2020. Novel coronavirus structure data

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+
Jul 12, 2017. Major update of PDB

-
Yorodumi