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データを開く
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基本情報
| 登録情報 | データベース: PDB / ID: 9btj | |||||||||||||||||||||||||||||||||
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| タイトル | Rhesus Fab 6561-a.01 in complex with HIV-1 Ce1176.A3 RnS SOSIP Env | |||||||||||||||||||||||||||||||||
要素 |
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キーワード | VIRAL PROTEIN/IMMUNE SYSTEM / HIV-1 / SOSIP / Vaccine / VIRAL PROTEIN-IMMUNE SYSTEM complex / V2-Apex / multi-donor / SHIV / IMMUNE SYSTEM | |||||||||||||||||||||||||||||||||
| 機能・相同性 | 機能・相同性情報symbiont-mediated perturbation of host defense response / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / host cell endosome membrane / clathrin-dependent endocytosis of virus by host cell / viral protein processing / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell ...symbiont-mediated perturbation of host defense response / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / host cell endosome membrane / clathrin-dependent endocytosis of virus by host cell / viral protein processing / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / structural molecule activity / membrane 類似検索 - 分子機能 | |||||||||||||||||||||||||||||||||
| 生物種 | ![]() ![]() Human immunodeficiency virus 1 (ヒト免疫不全ウイルス) | |||||||||||||||||||||||||||||||||
| 手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.22 Å | |||||||||||||||||||||||||||||||||
データ登録者 | Gorman, J. / Kwong, P.D. | |||||||||||||||||||||||||||||||||
| 資金援助 | 米国, 2件
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引用 | ジャーナル: J Exp Med / 年: 2025タイトル: Structural and genetic basis of HIV-1 envelope V2 apex recognition by rhesus broadly neutralizing antibodies. 著者: Ryan S Roark / Rumi Habib / Jason Gorman / Hui Li / Andrew Jesse Connell / Mattia Bonsignori / Yicheng Guo / Michael P Hogarty / Adam S Olia / Kirsten J Sowers / Baoshan Zhang / Frederic ...著者: Ryan S Roark / Rumi Habib / Jason Gorman / Hui Li / Andrew Jesse Connell / Mattia Bonsignori / Yicheng Guo / Michael P Hogarty / Adam S Olia / Kirsten J Sowers / Baoshan Zhang / Frederic Bibollet-Ruche / Tatsiana Bylund / Sean Callaghan / John W Carey / Gabriele Cerutti / Darcy R Harris / Wanting He / Emily Lewis / Tracy Liu / Rosemarie D Mason / Yujie Qiao / Younghoon Park / Juliette M Rando / Ajay Singh / Jeremy J Wolff / Q Paula Lei / Mark K Louder / Raiees Andrabi / Nicole A Doria-Rose / Kevin O Saunders / Michael S Seaman / Barton F Haynes / Daniel W Kulp / John R Mascola / Mario Roederer / Theodore C Pierson / Zizhang Sheng / Beatrice H Hahn / George M Shaw / Peter D Kwong / Lawrence Shapiro / ![]() 要旨: Broadly neutralizing antibodies targeting the V2 apex of HIV-1 envelope are desired as vaccine design templates, but few have been described. Here, we report 11 lineages of V2 apex-neutralizing ...Broadly neutralizing antibodies targeting the V2 apex of HIV-1 envelope are desired as vaccine design templates, but few have been described. Here, we report 11 lineages of V2 apex-neutralizing antibodies from simian-human immunodeficiency virus (SHIV)-infected rhesus macaques and determine cryo-EM structures for 9. A single V2 apex-neutralizing lineage accounted for cross-clade breadth in most macaques, and somatic hypermutation relative to breadth was generally low, exemplified by antibody V033-a.01 with <5% nucleotide mutation and 37% breadth (208-strain panel). Envelope complex structures revealed eight different antibody classes (one multi-donor) and the complete repertoire of all five possible recognition topologies, recapitulating canonical human modes of apex insertion and C-strand hydrogen bonding. Despite this diversity in recognition, all rhesus-V2 apex antibodies were derived from reading frame two of the DH3-15*01 gene. Collectively, these results define-in rhesus-the structural and genetic basis of HIV-1 V2 apex recognition and demonstrate unprecedented structural plasticity of a highly selected immunogenetic element. | |||||||||||||||||||||||||||||||||
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構造の表示
| 構造ビューア | 分子: Molmil Jmol/JSmol |
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ダウンロードとリンク
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ダウンロード
| PDBx/mmCIF形式 | 9btj.cif.gz | 418.9 KB | 表示 | PDBx/mmCIF形式 |
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| PDB形式 | pdb9btj.ent.gz | 342.7 KB | 表示 | PDB形式 |
| PDBx/mmJSON形式 | 9btj.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
| その他 | その他のダウンロード |
-検証レポート
| 文書・要旨 | 9btj_validation.pdf.gz | 3.8 MB | 表示 | wwPDB検証レポート |
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| 文書・詳細版 | 9btj_full_validation.pdf.gz | 3.9 MB | 表示 | |
| XML形式データ | 9btj_validation.xml.gz | 71 KB | 表示 | |
| CIF形式データ | 9btj_validation.cif.gz | 103.6 KB | 表示 | |
| アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/bt/9btj ftp://data.pdbj.org/pub/pdb/validation_reports/bt/9btj | HTTPS FTP |
-関連構造データ
| 関連構造データ | ![]() 44892MC ![]() 9bnkC ![]() 9bnlC ![]() 9bnmC ![]() 9bnpC ![]() 9bthC ![]() 9btiC ![]() 9btlC ![]() 9btvC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
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集合体
| 登録構造単位 | ![]()
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| 1 |
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要素
-Envelope glycoprotein ... , 2種, 6分子 GADBCE
| #3: タンパク質 | 分子量: 53648.887 Da / 分子数: 3 / 由来タイプ: 組換発現 由来: (組換発現) ![]() Human immunodeficiency virus 1 (ヒト免疫不全ウイルス)遺伝子: env / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: C6G0D7#4: タンパク質 | 分子量: 17240.510 Da / 分子数: 3 / 由来タイプ: 組換発現 由来: (組換発現) ![]() Human immunodeficiency virus 1 (ヒト免疫不全ウイルス)遺伝子: env / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: C6G0E7 |
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-抗体 , 2種, 2分子 LH
| #1: 抗体 | 分子量: 23076.508 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() Homo sapiens (ヒト) |
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| #2: 抗体 | 分子量: 27288.469 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() Homo sapiens (ヒト) |
-糖 , 9種, 84分子 
| #5: 多糖 | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose #6: 多糖 | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose #7: 多糖 | #8: 多糖 | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | #9: 多糖 | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | #10: 多糖 | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | #11: 多糖 | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | #12: 多糖 | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | #13: 糖 | ChemComp-NAG / |
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-詳細
| 研究の焦点であるリガンドがあるか | N |
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| Has protein modification | Y |
-実験情報
-実験
| 実験 | 手法: 電子顕微鏡法 |
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| EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
| 構成要素 | 名称: Rhesus Fab 6561-a.01 in complex with HIV-1 Ce1176.A3 RnS SOSIP Env タイプ: COMPLEX / Entity ID: #1-#2, #4, #3 / 由来: RECOMBINANT |
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| 分子量 | 実験値: NO |
| 由来(天然) | 生物種: ![]() Human immunodeficiency virus 1 (ヒト免疫不全ウイルス) |
| 由来(組換発現) | 生物種: Homo sapiens (ヒト) |
| 緩衝液 | pH: 7.4 / 詳細: PBS |
| 試料 | 濃度: 2 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
| 試料支持 | グリッドの材料: COPPER / グリッドのタイプ: C-flat-1.2/1.3 |
| 急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE / 湿度: 90 % / 凍結前の試料温度: 293 K |
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電子顕微鏡撮影
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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| 顕微鏡 | モデル: FEI TITAN KRIOS |
| 電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
| 電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2500 nm / 最小 デフォーカス(公称値): 1000 nm / C2レンズ絞り径: 70 µm |
| 撮影 | 平均露光時間: 10 sec. / 電子線照射量: 69.48 e/Å2 / 検出モード: COUNTING フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 撮影したグリッド数: 1 |
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解析
| EMソフトウェア | 名称: PHENIX / バージョン: dev_5278 / カテゴリ: モデル精密化 |
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| CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
| 対称性 | 点対称性: C1 (非対称) |
| 3次元再構成 | 解像度: 4.22 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 150641 / 対称性のタイプ: POINT |
| 原子モデル構築 | プロトコル: FLEXIBLE FIT / 空間: REAL |
| 原子モデル構築 | PDB-ID: 6VTT Accession code: 6VTT / Source name: PDB / タイプ: experimental model |
| 精密化 | 交差検証法: NONE |
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コントローラー
万見について






Human immunodeficiency virus 1 (ヒト免疫不全ウイルス)
米国, 2件
引用
















PDBj







Homo sapiens (ヒト)
FIELD EMISSION GUN
