+Open data
-Basic information
Entry | Database: PDB / ID: 8zfl | |||||||||
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Title | Caenorhabditis elegans ACR-23 in apo state | |||||||||
Components | Betaine receptor acr-23,Soluble cytochrome b562 | |||||||||
Keywords | TRANSPORT PROTEIN / ion channel | |||||||||
Function / homology | Function and homology information Highly calcium permeable postsynaptic nicotinic acetylcholine receptors / Neurotransmitter receptors and postsynaptic signal transmission / excitatory extracellular ligand-gated monoatomic ion channel activity / transmembrane transporter complex / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / electron transport chain / transmembrane signaling receptor activity / postsynapse / periplasmic space / electron transfer activity ...Highly calcium permeable postsynaptic nicotinic acetylcholine receptors / Neurotransmitter receptors and postsynaptic signal transmission / excitatory extracellular ligand-gated monoatomic ion channel activity / transmembrane transporter complex / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / electron transport chain / transmembrane signaling receptor activity / postsynapse / periplasmic space / electron transfer activity / neuron projection / iron ion binding / synapse / heme binding / plasma membrane Similarity search - Function | |||||||||
Biological species | Caenorhabditis elegans (invertebrata) Shimwellia blattae (bacteria) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.61 Å | |||||||||
Authors | Chen, Q.F. / Liu, F.L. / Li, T.Y. / Gong, H.H. / Guo, F. / Liu, S. | |||||||||
Funding support | China, 2items
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Citation | Journal: To Be Published Title: Structural basis of acetylcholine receptor like-23 (ACR-23) activation by anthelmintics monepantel and betaine Authors: Liu, F.L. / Li, T.Y. / Gong, H.H. / Guo, F. / Liu, S. / Chen, Q.F. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8zfl.cif.gz | 428.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8zfl.ent.gz | 348.7 KB | Display | PDB format |
PDBx/mmJSON format | 8zfl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zf/8zfl ftp://data.pdbj.org/pub/pdb/validation_reports/zf/8zfl | HTTPS FTP |
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-Related structure data
Related structure data | 60064MC 8zfkC 8zfmC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 70582.281 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Details: Residues 387-500 correspond to the bril expression tag. Residues 599-603 and 610-612 correspond to flexible linkers, residues 604-609 correspond to the thrombin cleavage site, and residues ...Details: Residues 387-500 correspond to the bril expression tag. Residues 599-603 and 610-612 correspond to flexible linkers, residues 604-609 correspond to the thrombin cleavage site, and residues 613-620 correspond to the strep ll tag. Source: (gene. exp.) Caenorhabditis elegans (invertebrata), (gene. exp.) Shimwellia blattae (bacteria) Gene: acr-23, 5E130, F59B1.9, cybC Production host: Spodoptera aff. frugiperda 1 BOLD-2017 (butterflies/moths) References: UniProt: G5EG88, UniProt: P0ABE7 #2: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #3: Sugar | ChemComp-NAG / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: acetylcholine receptor like-23(ACR-23) / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Molecular weight | Value: 0.067 MDa / Experimental value: NO |
Source (natural) | Organism: Caenorhabditis elegans (invertebrata) |
Source (recombinant) | Organism: Spodoptera aff. frugiperda 1 BOLD-2017 (butterflies/moths) Cell: sf9 |
Buffer solution | pH: 8 |
Specimen | Conc.: 4 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company | ||||||||||||
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Microscopy | Model: TFS KRIOS | ||||||||||||
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM | ||||||||||||
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2400 nm / Nominal defocus min: 1200 nm | ||||||||||||
Image recording |
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-Processing
Image processing |
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CTF correction |
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3D reconstruction | Resolution: 2.61 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 402991 / Symmetry type: POINT | ||||||||||||
Refinement | Highest resolution: 2.61 Å |