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- PDB-8zfk: Caenorhabditis elegans ACR-23 in betaine and monepantel bound state -
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Open data
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Basic information
Entry | Database: PDB / ID: 8zfk | |||||||||
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Title | Caenorhabditis elegans ACR-23 in betaine and monepantel bound state | |||||||||
![]() | Betaine receptor acr-23,Soluble cytochrome b562 | |||||||||
![]() | TRANSPORT PROTEIN / ion channel | |||||||||
Function / homology | ![]() Highly calcium permeable postsynaptic nicotinic acetylcholine receptors / Neurotransmitter receptors and postsynaptic signal transmission / excitatory extracellular ligand-gated monoatomic ion channel activity / transmembrane transporter complex / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / electron transport chain / transmembrane signaling receptor activity / postsynapse / periplasmic space / electron transfer activity ...Highly calcium permeable postsynaptic nicotinic acetylcholine receptors / Neurotransmitter receptors and postsynaptic signal transmission / excitatory extracellular ligand-gated monoatomic ion channel activity / transmembrane transporter complex / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / electron transport chain / transmembrane signaling receptor activity / postsynapse / periplasmic space / electron transfer activity / neuron projection / iron ion binding / synapse / heme binding / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.64 Å | |||||||||
![]() | Chen, Q.F. / Liu, F.L. / Li, T.Y. / Gong, H.H. / Guo, F. / Liu, S. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis of acetylcholine receptor like-23 (ACR-23) activation by anthelmintics monepantel and betaine Authors: Liu, F.L. / Li, T.Y. / Gong, H.H. / Guo, F. / Liu, S. / Chen, Q.F. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 439.9 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 60063MC ![]() 8zflC ![]() 8zfmC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
-Protein , 1 types, 5 molecules DABCE
#1: Protein | Mass: 70582.281 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Details: Residues 387-500 correspond to the bril expression tag, whereas resides 604-609 correspond to the thrombin cleavage site, resides 599-603 and 610-612 correspond to flexible linkers, resides ...Details: Residues 387-500 correspond to the bril expression tag, whereas resides 604-609 correspond to the thrombin cleavage site, resides 599-603 and 610-612 correspond to flexible linkers, resides 613-620 correspond to the strep ll tag. Source: (gene. exp.) ![]() ![]() ![]() ![]() Gene: acr-23, 5E130, F59B1.9, cybC Production host: ![]() References: UniProt: G5EG88, UniProt: P0ABE7 |
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-Sugars , 2 types, 15 molecules ![](data/chem/img/NAG.gif)
#2: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #3: Sugar | ChemComp-NAG / |
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-Non-polymers , 3 types, 25 molecules ![](data/chem/img/BET.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-A1D8E / ~{ Mass: 473.392 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C20H13F6N3O2S #5: Chemical | ChemComp-BET / #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: acetylcholine receptor like-23(ACR-23) / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Molecular weight | Value: 0.067 MDa / Experimental value: NO |
Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() Cell: sf9 |
Buffer solution | pH: 8 |
Specimen | Conc.: 4 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: TFS KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2400 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K2 BASE (4k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 2.64 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 219723 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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