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- PDB-8z1e: A homotrimeric GPCR architecture of the human cytomegalovirus (UL... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8z1e | ||||||
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Title | A homotrimeric GPCR architecture of the human cytomegalovirus (UL78) revealed by cryo-EM | ||||||
![]() | Uncharacterized protein UL78 | ||||||
![]() | ANTIVIRAL PROTEIN / human cytomegalovirus (HCMV) / UL78 / homotrimeric architecture / STRUCTURAL PROTEIN | ||||||
Function / homology | GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile. / membrane / Uncharacterized protein UL78![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.12 Å | ||||||
![]() | Chen, Y. / Li, Y. / Zhou, Q. / Cong, Z. / Lin, S. / Yan, J. / Chen, X. / Yang, D. / Ying, T. / Wang, M.-W. | ||||||
Funding support | ![]()
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![]() | ![]() Title: A homotrimeric GPCR architecture of the human cytomegalovirus revealed by cryo-EM. Authors: Yanyan Chen / Yang Li / Qingtong Zhou / Zhaotong Cong / Shi Lin / Jiahui Yan / Xianyue Chen / Dehua Yang / Tianlei Ying / Ming-Wei Wang / ![]() ![]() | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 165.4 KB | Display | ![]() |
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PDB format | ![]() | 131.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 32.3 KB | Display | |
Data in CIF | ![]() | 45.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 39724MC ![]() 39613 M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 31873.582 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: A homotrimeric GPCR architecture of the human cytomegalovirus revealed by cryo-EM Type: COMPLEX / Entity ID: all / Source: NATURAL |
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Source (natural) | Organism: ![]() |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: OTHER / Accelerating voltage: 300 kV / Illumination mode: OTHER |
Electron lens | Mode: OTHER / Nominal defocus max: 2200 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 80 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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Symmetry | Point symmetry: C3 (3 fold cyclic) | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.12 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 85264 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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