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- EMDB-39724: A homotrimeric GPCR architecture of the human cytomegalovirus (UL... -
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Open data
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Basic information
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Title | A homotrimeric GPCR architecture of the human cytomegalovirus (UL78) revealed by cryo-EM | |||||||||
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![]() | human cytomegalovirus (HCMV) / UL78 / homotrimeric architecture / STRUCTURAL PROTEIN / ANTIVIRAL PROTEIN | |||||||||
Function / homology | GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile. / membrane / Uncharacterized protein UL78![]() | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.12 Å | |||||||||
![]() | Chen Y / Li Y / Zhou Q / Cong Z / Lin S / Yan J / Chen X / Yang D / Ying T / Wang M-W | |||||||||
Funding support | ![]()
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![]() | ![]() Title: A homotrimeric GPCR architecture of the human cytomegalovirus revealed by cryo-EM. Authors: Yanyan Chen / Yang Li / Qingtong Zhou / Zhaotong Cong / Shi Lin / Jiahui Yan / Xianyue Chen / Dehua Yang / Tianlei Ying / Ming-Wei Wang / ![]() ![]() | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 59.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.2 KB 14.2 KB | Display Display | ![]() |
Images | ![]() | 82.3 KB | ||
Filedesc metadata | ![]() | 5.2 KB | ||
Others | ![]() ![]() | 59.5 MB 59.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 889.5 KB | Display | ![]() |
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Full document | ![]() | 889 KB | Display | |
Data in XML | ![]() | 12.3 KB | Display | |
Data in CIF | ![]() | 14.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8z1eMC ![]() 39613 M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.071 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_39724_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_39724_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : A homotrimeric GPCR architecture of the human cytomegalovirus rev...
Entire | Name: A homotrimeric GPCR architecture of the human cytomegalovirus revealed by cryo-EM |
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Components |
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-Supramolecule #1: A homotrimeric GPCR architecture of the human cytomegalovirus rev...
Supramolecule | Name: A homotrimeric GPCR architecture of the human cytomegalovirus revealed by cryo-EM type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Uncharacterized protein UL78
Macromolecule | Name: Uncharacterized protein UL78 / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 31.873582 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: SADRAASDLL IGMFGSVSLV NLLTIIGCLW VLRVTRPPVS VMIFTWNLVL SQFFSILATM LSKGIMLRGA LNLSLCRLVL FVDDVGLYS TALFFLFLIL DRLSAISYGR DLWHHETREN AGVALYAVAF AWVLSIVAAV PTAATGSLDY RWLGCQIPIQ Y AAVDLTIK ...String: SADRAASDLL IGMFGSVSLV NLLTIIGCLW VLRVTRPPVS VMIFTWNLVL SQFFSILATM LSKGIMLRGA LNLSLCRLVL FVDDVGLYS TALFFLFLIL DRLSAISYGR DLWHHETREN AGVALYAVAF AWVLSIVAAV PTAATGSLDY RWLGCQIPIQ Y AAVDLTIK MWFLLGAPMI AVLANVVELA YSDRRDHVWS YVGRVCTFYV TCLMLFVPYY CFRVLRGVLQ PASAAGTGFG IM DYVELAT RTLLTMRLGI LPLFIIAFFS REPTKDLDDS FDYLVERC UniProtKB: Uncharacterized protein UL78 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 80.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: OTHER |
Electron optics | Illumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Applied symmetry - Point group: C3 (3 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 3.12 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 85264 |
Initial angle assignment | Type: OTHER |
Final angle assignment | Type: OTHER |