+Open data
-Basic information
Entry | Database: PDB / ID: 8yaa | ||||||
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Title | Cryo-EM structure of MIK2-SCOOP12-BAK1 | ||||||
Components |
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Keywords | TRANSFERASE/PLANT PROTEIN / LRR-RLK / SERK / MIK2 / SCOOP / BAK1 / PLANT PROTEIN / TRANSFERASE / PLANT PROTEIN complex / TRANSFERASE-PLANT PROTEIN complex | ||||||
Function / homology | Function and homology information plant-type cell wall organization / indole glucosinolate biosynthetic process / regulation of unidimensional cell growth / positive regulation of defense response / regulation of root development / regulation of defense response to fungus / pollen tube / cell-cell signaling involved in cell fate commitment / regulation of defense response to bacterium / pollen tube guidance ...plant-type cell wall organization / indole glucosinolate biosynthetic process / regulation of unidimensional cell growth / positive regulation of defense response / regulation of root development / regulation of defense response to fungus / pollen tube / cell-cell signaling involved in cell fate commitment / regulation of defense response to bacterium / pollen tube guidance / response to herbivore / regulation of root meristem growth / defense response to insect / response to insect / peptide receptor activity / LRR domain binding / jasmonic acid biosynthetic process / cellular response to peptide / apoplast / plasmodesma / receptor serine/threonine kinase binding / regulation of reactive oxygen species metabolic process / negative regulation of reactive oxygen species metabolic process / transmembrane receptor protein tyrosine kinase activity / response to bacterium / peptide binding / response to molecule of bacterial origin / receptor protein-tyrosine kinase / defense response / response to wounding / non-specific serine/threonine protein kinase / endosome membrane / signaling receptor binding / protein serine kinase activity / protein serine/threonine kinase activity / ATP binding / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.34 Å | ||||||
Authors | Jia, F.S. / Xiao, Y. / Chai, J.J. | ||||||
Funding support | China, 1items
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Citation | Journal: Nat.Plants / Year: 2024 Title: N-glycosylation facilitates the activation of a plant cell-surface receptor Authors: Jia, F. / Xiao, Y. / Feng, Y. / Yan, J. / Fan, M. / Sun, Y. / Huang, S. / Li, W. / Zhao, T. / Han, Z. / Hou, S. / Chai, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8yaa.cif.gz | 170.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8yaa.ent.gz | 131.9 KB | Display | PDB format |
PDBx/mmJSON format | 8yaa.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8yaa_validation.pdf.gz | 1.7 MB | Display | wwPDB validaton report |
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Full document | 8yaa_full_validation.pdf.gz | 1.7 MB | Display | |
Data in XML | 8yaa_validation.xml.gz | 36.3 KB | Display | |
Data in CIF | 8yaa_validation.cif.gz | 52 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ya/8yaa ftp://data.pdbj.org/pub/pdb/validation_reports/ya/8yaa | HTTPS FTP |
-Related structure data
Related structure data | 39093MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-Protein , 2 types, 2 molecules CA
#1: Protein | Mass: 19160.684 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: BAK1, ELG, SERK3, At4g33430, F17M5.190 / Production host: Trichoplusia ni (cabbage looper) References: UniProt: Q94F62, receptor protein-tyrosine kinase, non-specific serine/threonine protein kinase |
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#2: Protein | Mass: 71858.742 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: MIK2, At4g08850, T32A17.160 / Production host: Trichoplusia ni (cabbage looper) References: UniProt: Q8VZG8, non-specific serine/threonine protein kinase |
-Protein/peptide , 1 types, 1 molecules B
#3: Protein/peptide | Mass: 1318.399 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Arabidopsis thaliana (thale cress) / References: UniProt: B3H7I1 |
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-Sugars , 4 types, 20 molecules
#4: Polysaccharide | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | ||||
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#5: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose #6: Polysaccharide | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | #7: Sugar | ChemComp-NAG / |
-Details
Has ligand of interest | N |
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Has protein modification | Y |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Ternary complex of MIK2-SCOOP12-BAK1 / Type: COMPLEX / Entity ID: #1-#3 / Source: RECOMBINANT |
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Source (natural) | Organism: Arabidopsis (plant) |
Source (recombinant) | Organism: Trichoplusia ni (cabbage looper) |
Buffer solution | pH: 6 |
Specimen | Conc.: 1 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 3.34 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 319127 / Symmetry type: POINT |
Refinement | Highest resolution: 3.34 Å |