+
Open data
-
Basic information
Entry | Database: PDB / ID: 8y6q | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of the Dark/Dronc complex | ||||||
![]() |
| ||||||
![]() | APOPTOSIS / Dark / Dronc / cryo-EM | ||||||
Function / homology | ![]() hemocyte development / TP53 Regulates Transcription of Caspase Activators and Caspases / nurse cell apoptotic process / negative regulation of humoral immune response / positive regulation of glial cell apoptotic process / Formation of apoptosome / head involution / embryonic development via the syncytial blastoderm / salivary gland histolysis / positive regulation of compound eye retinal cell programmed cell death ...hemocyte development / TP53 Regulates Transcription of Caspase Activators and Caspases / nurse cell apoptotic process / negative regulation of humoral immune response / positive regulation of glial cell apoptotic process / Formation of apoptosome / head involution / embryonic development via the syncytial blastoderm / salivary gland histolysis / positive regulation of compound eye retinal cell programmed cell death / melanization defense response / sarcosine catabolic process / Activation of caspases through apoptosome-mediated cleavage / Regulation of the apoptosome activity / central nervous system formation / metamorphosis / compound eye development / chaeta development / sperm individualization / apoptosome / autophagic cell death / programmed cell death involved in cell development / Neutrophil degranulation / programmed necrotic cell death / S-adenosylmethionine cycle / CARD domain binding / programmed cell death / cysteine-type endopeptidase activity involved in apoptotic signaling pathway / triglyceride homeostasis / zymogen activation / dendrite morphogenesis / neuron remodeling / response to starvation / cysteine-type endopeptidase activator activity involved in apoptotic process / protein autoprocessing / ectopic germ cell programmed cell death / regulation of autophagy / central nervous system development / determination of adult lifespan / response to gamma radiation / ADP binding / neuron cellular homeostasis / endopeptidase activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / positive regulation of apoptotic process / negative regulation of cell population proliferation / cysteine-type endopeptidase activity / apoptotic process / protein homodimerization activity / ATP binding / identical protein binding / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 7 Å | ||||||
![]() | Tian, L. / Li, Y. / Shi, Y. | ||||||
Funding support | 1items
| ||||||
![]() | ![]() Title: Dark and Dronc activation in . Authors: Lu Tian / Yini Li / Yigong Shi / ![]() Abstract: The onset of apoptosis is characterized by a cascade of caspase activation, where initiator caspases are activated by a multimeric adaptor complex known as the apoptosome. In , the initiator caspase ...The onset of apoptosis is characterized by a cascade of caspase activation, where initiator caspases are activated by a multimeric adaptor complex known as the apoptosome. In , the initiator caspase Dronc undergoes autocatalytic activation in the presence of the Dark apoptosome. Despite rigorous investigations, the activation mechanism for Dronc remains elusive. Here, we report the cryo-EM structures of an auto-inhibited Dark monomer and a single-layered, multimeric Dark/Dronc complex. Our biochemical analysis suggests that the auto-inhibited Dark oligomerizes upon binding to Dronc, which is sufficient for the activation of both Dark and Dronc. In contrast, the previously observed double-ring Dark apoptosome may represent a non-functional or "off-pathway" conformation. These findings expand our understanding on the molecular mechanism of apoptosis in . | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 1.6 MB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 1.3 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.8 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 2.4 MB | Display | |
Data in XML | ![]() | 328.3 KB | Display | |
Data in CIF | ![]() | 476.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 38995MC ![]() 8y6pC M: map data used to model this data C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
|
---|---|
1 |
|
-
Components
#1: Protein | Mass: 142710.344 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: Dark, anon-53Fa, Apaf, APAF-1, Apaf-1, apaf-1, APAF1, Apaf1, apaf1, arc, ARK, Ark, ark, Ark-RB, D-Apaf-1, dapaf, Dapaf-1, dAPAF-1, dApaf-1, dapaf-1, dApaf-1/DARK/HAC-1, Dapaf-1/HAC-1, dapaf-1L, ...Gene: Dark, anon-53Fa, Apaf, APAF-1, Apaf-1, apaf-1, APAF1, Apaf1, apaf1, arc, ARK, Ark, ark, Ark-RB, D-Apaf-1, dapaf, Dapaf-1, dAPAF-1, dApaf-1, dapaf-1, dApaf-1/DARK/HAC-1, Dapaf-1/HAC-1, dapaf-1L, dapaf-1S, dApaf1, DARK, dArk, dark, Dark/Apaf-I, dark/dapaf-1/hac-1, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, dark/hac-1/dapaf-1, Dark/Hac-1/dApaf1, Dmel\CG6829, Hac-1, hac-1, Hac-1/Dark, Hac1, hac1, l(2)SH0173, T1, CG6829, Dmel_CG6829 Production host: ![]() ![]() #2: Protein | Mass: 12039.956 Da / Num. of mol.: 8 / Fragment: CARD Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q9XYF4, Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases Has ligand of interest | N | |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-
Sample preparation
Component |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Source (natural) | Organism: ![]() ![]() | ||||||||||||||||||||||||
Source (recombinant) |
| ||||||||||||||||||||||||
Buffer solution | pH: 8 | ||||||||||||||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||||||||||||||
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-
Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
3D reconstruction | Resolution: 7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 2710 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
|