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- PDB-8xtg: Crystal structure of methyltransferase MpaG' in complex with SAH ... -

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Basic information

Entry
Database: PDB / ID: 8xtg
TitleCrystal structure of methyltransferase MpaG' in complex with SAH and DMMPA
ComponentsO-methyltransferase mpaG'
KeywordsBIOSYNTHETIC PROTEIN / methyltransferase / MpaG' / MPA biosynthesis
Function / homology
Function and homology information


mycophenolic acid biosynthetic process / polyketide synthase activity / terpenoid biosynthetic process / O-methyltransferase activity / Transferases; Transferring one-carbon groups; Methyltransferases / methyltransferase activity / methylation / cytosol
Similarity search - Function
O-methyltransferase domain / O-methyltransferase COMT-type / O-methyltransferase domain / SAM-dependent O-methyltransferase class II-type profile. / N-6 Adenine-specific DNA methylases signature. / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / S-adenosyl-L-methionine-dependent methyltransferase superfamily
Similarity search - Domain/homology
: / S-ADENOSYL-L-HOMOCYSTEINE / O-methyltransferase mpaG'
Similarity search - Component
Biological speciesPenicillium brevicompactum (fungus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å
AuthorsYou, C. / Pan, Y.J. / Li, S.Y. / Feng, Y.G.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32070125 China
CitationJournal: Protein Sci. / Year: 2024
Title: Structural basis for substrate flexibility of the O-methyltransferase MpaG' involved in mycophenolic acid biosynthesis.
Authors: You, C. / Pan, Y. / Liu, R. / Li, S. / Feng, Y.
History
DepositionJan 10, 2024Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Aug 28, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: O-methyltransferase mpaG'
B: O-methyltransferase mpaG'
C: O-methyltransferase mpaG'
D: O-methyltransferase mpaG'
E: O-methyltransferase mpaG'
F: O-methyltransferase mpaG'
hetero molecules


Theoretical massNumber of molelcules
Total (without water)266,30318
Polymers262,1586
Non-polymers4,14412
Water31,9951776
1
A: O-methyltransferase mpaG'
B: O-methyltransferase mpaG'
hetero molecules


Theoretical massNumber of molelcules
Total (without water)88,7686
Polymers87,3862
Non-polymers1,3814
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8910 Å2
ΔGint-68 kcal/mol
Surface area29440 Å2
MethodPISA
2
C: O-methyltransferase mpaG'
D: O-methyltransferase mpaG'
hetero molecules


Theoretical massNumber of molelcules
Total (without water)88,7686
Polymers87,3862
Non-polymers1,3814
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9140 Å2
ΔGint-70 kcal/mol
Surface area29380 Å2
MethodPISA
3
E: O-methyltransferase mpaG'
F: O-methyltransferase mpaG'
hetero molecules


Theoretical massNumber of molelcules
Total (without water)88,7686
Polymers87,3862
Non-polymers1,3814
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9010 Å2
ΔGint-70 kcal/mol
Surface area28990 Å2
MethodPISA
Unit cell
Length a, b, c (Å)199.541, 199.541, 67.154
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number145
Space group name H-MP32
Space group name HallP32

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Components

#1: Protein
O-methyltransferase mpaG' / Mycophenolic acid biosynthesis cluster protein G'


Mass: 43693.082 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Penicillium brevicompactum (fungus) / Strain: NRRL 864 / Gene: mpaG' / Plasmid: pETM-11 / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: A0A0B5L781, Transferases; Transferring one-carbon groups; Methyltransferases
#2: Chemical
ChemComp-SAH / S-ADENOSYL-L-HOMOCYSTEINE


Type: L-peptide linking / Mass: 384.411 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C14H20N6O5S / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical
ChemComp-A1LWD / O-desmethyl mycophenolic acid / (~{E})-4-methyl-6-[7-methyl-4,6-bis(oxidanyl)-3-oxidanylidene-1~{H}-2-benzofuran-5-yl]hex-4-enoic acid


Mass: 306.311 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C16H18O6 / Feature type: SUBJECT OF INVESTIGATION
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1776 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.94 Å3/Da / Density % sol: 58.22 %
Crystal growTemperature: 289 K / Method: vapor diffusion
Details: 15% PEG3350 (w/v), 0.1 M sodium cacodylate, pH 6.0 and 0.2 M magnesium chloride

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.97853 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 14, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97853 Å / Relative weight: 1
ReflectionResolution: 2→47.97 Å / Num. obs: 202861 / % possible obs: 99.9 % / Redundancy: 5.2 % / Biso Wilson estimate: 23.29 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.131 / Rpim(I) all: 0.064 / Rrim(I) all: 0.146 / Χ2: 0.97 / Net I/σ(I): 10.8
Reflection shellResolution: 2→2.03 Å / % possible obs: 98.2 % / Redundancy: 4.9 % / Rmerge(I) obs: 0.942 / Num. measured all: 48859 / Num. unique obs: 9929 / CC1/2: 0.666 / Rpim(I) all: 0.472 / Rrim(I) all: 1.056 / Χ2: 0.9 / Net I/σ(I) obs: 1.8

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
Aimlessdata scaling
autoPROCdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: AlphaFold

Resolution: 2→47.93 Å / Cross valid method: FREE R-VALUE / σ(F): 164.13 / Phase error: 35.2064
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflectionSelection details
Rfree0.2112 10417 5.14 %RANDOM
Rwork0.1618 192412 --
obs0.1649 202829 99.88 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 30.46 Å2
Refinement stepCycle: LAST / Resolution: 2→47.93 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms18239 0 288 1776 20303
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.003418983
X-RAY DIFFRACTIONf_angle_d0.651625822
X-RAY DIFFRACTIONf_chiral_restr0.04322814
X-RAY DIFFRACTIONf_plane_restr0.00563391
X-RAY DIFFRACTIONf_dihedral_angle_d5.53792748
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2-2.030.26255280.2329487X-RAY DIFFRACTION93.09
2.03-2.070.25534980.22129643X-RAY DIFFRACTION95.06
2.07-2.110.28264740.21459708X-RAY DIFFRACTION95.34
2.11-2.150.26895410.20949604X-RAY DIFFRACTION94.64
2.15-2.20.25685260.20259570X-RAY DIFFRACTION94.72
2.2-2.250.25055280.19349665X-RAY DIFFRACTION94.81
2.25-2.310.25544660.18969684X-RAY DIFFRACTION95.4
2.31-2.370.25655580.18499565X-RAY DIFFRACTION94.45
2.37-2.440.25475410.1839566X-RAY DIFFRACTION94.62
2.44-2.520.22224990.1769697X-RAY DIFFRACTION95.11
2.52-2.610.2164900.17419646X-RAY DIFFRACTION95.16
2.61-2.710.23915070.16969628X-RAY DIFFRACTION95
2.71-2.830.21195100.16579703X-RAY DIFFRACTION95.01
2.83-2.980.20875480.15899557X-RAY DIFFRACTION94.58
2.98-3.170.20085490.15269642X-RAY DIFFRACTION94.58
3.17-3.410.20365300.14019594X-RAY DIFFRACTION94.76
3.41-3.760.1855670.13429599X-RAY DIFFRACTION94.36
3.76-4.30.15525020.12149634X-RAY DIFFRACTION95.03
4.3-5.420.18175770.12959552X-RAY DIFFRACTION94.21
5.42-47.930.21654710.18039675X-RAY DIFFRACTION95.21
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
10.288258787238-0.00724575677666-0.2224198026250.852579829682-0.1216419259420.941823721971-0.01896200156210.0295472623363-0.07749244932150.0166646524822-0.002212311059650.07243698420170.164117367072-0.2157006665010.01156414940860.304843348938-0.0663035096539-0.02037992242910.303121337447-0.01138968170620.087534103894435.5061456245-0.6024743024961.56188559581
20.6675203844780.5376774716670.3631114339861.394519141420.7435166502960.9709024436030.000728627666633-0.00270741209781-0.03983639586460.0031475095074-0.0232929173315-0.03084388112190.0501451787083-0.03823140178870.02261104798810.235932276291-0.0361240806696-0.002865736518810.2447689241530.006146811028890.084968742879739.7065845644-16.5742362463-20.6539951049
32.94601593193-1.69575603196-1.281661297745.655328333453.500108595333.20739344268-0.450502918813-0.281794016797-0.1941108504630.5038279946010.086267560190.564383825520.105638903565-0.5166868388190.3285537979150.3102189434950.0285457442960.02070269807860.637080539706-0.01561311347480.20087118185415.31375198334.753630414674.31681799453
40.154575103656-0.0361026799849-0.08204057750340.2514526110950.2098670152520.5049884073720.02947386488760.04649561431990.00473742507133-0.0764453552441-0.02816340191380.0200635413986-0.0217441092754-0.09977968638440.001055439987480.232662539674-0.0205067056106-0.01201100092020.2759494543440.01639826079020.086097096141136.008726009918.60091168330.27490507037
55.36321313567-1.95904732523-0.915304469611.06179189770.6095457336070.4049732166580.3982067626880.3865721019580.601660248409-0.368869549772-0.0870881516694-0.258500169782-0.275979693298-0.0900643962632-0.1752667700650.26019321758-0.01914706009920.001216925914790.3560693015840.06793250468380.13322972942234.433611505639.819717135511.2591357344
60.344972344215-0.12976080845-0.4797402877280.8822100319890.4132661651221.11575658305-0.0327720279078-0.03147773477190.06624102510710.09045323128510.02149757905560.08777939950490.1357399682620.0608802321584-0.0008762992295080.262386520023-0.0041241901293-0.02404726246950.3360483135930.01224761253510.060332796804133.033709515622.327672935623.0937032805
70.747557157755-0.05758188141660.2501092867530.3522338347540.02842272021660.609894593854-0.003880649401210.0258244533190.06037944184080.00473164272321-0.0152501872525-0.0251226540313-0.06703654319280.09802199135730.01776886278380.240039091878-0.01971485192050.001171820583940.2844127207950.01261323723260.086963160563771.290494706933.6226795528-4.30764225012
80.894014939352-0.4214097758480.207848790651.64049847873-0.3061926879330.638958035553-0.010006040150.07404660038560.00986430582443-0.08418449639240.008315181560860.0669748463986-0.1464206565850.08046501871840.0001993767034560.258302027482-0.04595721113070.002413763775480.2422108022960.01072010947690.079980035012959.951831414944.5050427287-26.9246979351
97.01765994884-2.567597155463.562073295334.7602969455-1.998289848792.15740826385-0.626503263695-0.5536804146450.6463688955330.4329035084650.17681176902-0.577819612103-0.5220154627950.4348009150790.3941345908660.405724117474-0.0894690383176-0.07945459511410.5365993692740.02602653889730.24226347597592.043463908543.6521791252-0.684411200131
100.493085766-0.1138601254440.1954145282840.282082097019-0.1255611016920.1823353542810.01880481052190.130730791315-0.0588850311514-0.0449036396807-0.0231252038851-0.001818526539870.03684451539170.1211082142220.01486957609190.225973250995-0.00426402736040.004697632250350.349156447625-0.02144430709270.085396481220985.816968687919.4438693418-4.57437395165
115.479441739872.27436577837-0.1214022392891.251576330130.3380633418610.499361821087-0.1386986855490.767371867574-0.762126430008-0.1505302731910.166651513715-0.173737182889-0.005947217006570.2174598703280.00714540092240.2450114565380.0468491826196-0.03911862685220.381382642196-0.06212147159760.179113695072101.3597154254.82627479666.50617280406
120.708696931005-0.3131155753440.2810673227010.640235279139-0.5550763365880.9187671938610.0501953033978-0.0195518723278-0.02120952261650.0938827523775-0.06467725699710.00253652276522-0.107830560969-0.008674643450790.01654352736960.244469227005-0.006579418783920.004624394779180.336710594477-0.01871113473270.093604005704390.557800062218.569312374618.4493868038
137.107105962763.43257921013-3.397994144635.84344931577-1.928390522782.81061539998-0.307128106112-1.12405242014-0.7527478279340.221955373777-0.246453218835-0.6036261867250.0878071733090.005179542491890.5141185563450.6313786695420.172136154268-0.05590928320750.5699733882010.02689912029790.33781296524288.9966565333-45.06610765881.45099584341
145.970790291851.23281839618-0.1546939447940.310886631352-0.01126811673480.0222232383634-0.08834780894390.169543270257-0.2048347906710.1052220058020.0611643736155-0.00813114690805-0.09173720649370.1755771536270.01771077560750.5791339128660.009584344742920.009179518915810.437200567989-0.004966240942840.19977873900383.8306890554-24.1434661765-8.89567431806
152.305983773120.648469756442-0.2359678074740.459473474164-0.8859296616752.70040169259-0.2350219033330.309093308535-0.0423041923873-0.1418118543220.298091153114-0.025556970848-0.202714366905-0.622495074072-0.05071927688440.47669248032-0.09807833594240.02792933775050.663054694073-0.02655947828670.22889057752972.8720191262-30.7488743799-22.4101043736
161.431458536040.6930361877021.086833755152.000527586520.04111125547120.9823464137080.07692585601920.212187520705-0.0612720797582-0.402602764797-0.0706928860436-0.0937459437745-0.0884411054788-0.186036039771-0.02007507942510.4560238044120.01929884216720.03970633195450.4783637441930.02215334508170.16664668885172.4951256056-32.6434612523-12.2256005219
172.909832205581.937029789552.017274067672.146247099071.294535909041.49353373882-0.064318589477-0.8414183801520.4244889078960.132022801149-0.1762448670840.346207620963-0.0401942539722-0.1025833067090.2099025246120.4631528957760.00873310545010.06991694860930.505715331062-0.06068049399660.29077302559374.6704079968-12.47728378539.06865298103
180.5363236157560.0748491787596-0.2017036916922.12385866046-2.227616275322.3381019902-0.02001489908670.0301080355225-0.0959646415951-0.1525066150760.02705945381330.01735766127470.3027259921410.1375803356820.02298508264390.4974323434290.00571633510515-0.02897956695350.456641740523-0.03741285863780.18659804752264.4119116875-32.91798600717.02576236944
191.67311632912-0.0257946166964-0.8147846715777.73083819619-1.267903685080.6978898033860.0517765473250.34315225072-0.00274454629272-0.04576624682640.04689504833360.379629158618-0.050938649909-0.0798027780133-0.1049781113090.37189871422-0.0188006339226-0.03902244958960.4347551927840.0153880361150.17328763768153.0569473767-40.253091624311.9566684547
202.05499018717-0.154143494953-1.433575803310.9872469576310.297183916651.02459617688-0.0111166627232-0.32530442140.01952012268020.1085812398430.05758887546770.077080398385-0.02526720656790.188933971005-0.04083764562680.423191318980.04898722010250.02576678408950.4528178152980.02026376067370.17270089423763.5218360976-33.19008455921.8184816804
211.913963537441.24444322497-0.7501259984813.16460352231-2.070639414991.38631612069-0.0727391503784-0.3081071380680.0512311695743-0.0314947592008-0.19628415924-0.07917711477050.2285091637140.3865411558290.2586039152510.446741978590.01717597072460.001581725007110.5167053732150.01817123081290.18417126705472.6185224016-33.246593003419.5950007458
221.574899126730.336749413988-0.02866161219521.723193789740.006309995423951.598356524920.0265162714773-0.0726220078739-0.0227303474266-0.0277536299902-0.0154356180223-0.1303623958350.05211030321250.407589323625-0.0159295789510.4142023181760.03723949316630.02961749559360.543368851821-0.01145328782990.16260624815188.9008816208-23.7111364775-0.956287684867
233.40230148306-0.145309927081-1.497763541740.711976164417-0.2315717462010.9891266559980.0723964622480.07345177983840.1100519870980.0026680934911-0.01841595617580.00565352779025-0.0165584479859-0.129149456756-0.04440287978540.386494144053-0.008784272544840.006367238891520.403301700384-0.01931572503440.169572576036102.581405689-14.9649574734-23.4496997339
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 5 through 181 )AA5 - 1811 - 177
22chain 'A' and (resid 182 through 398 )AA182 - 398178 - 394
33chain 'B' and (resid 4 through 42 )BD4 - 421 - 39
44chain 'B' and (resid 43 through 252 )BD43 - 25240 - 249
55chain 'B' and (resid 253 through 284 )BD253 - 284250 - 281
66chain 'B' and (resid 285 through 398 )BD285 - 398282 - 395
77chain 'C' and (resid 6 through 201 )CG6 - 2011 - 196
88chain 'C' and (resid 202 through 398 )CG202 - 398197 - 393
99chain 'D' and (resid 4 through 42 )DJ4 - 421 - 39
1010chain 'D' and (resid 43 through 252 )DJ43 - 25240 - 249
1111chain 'D' and (resid 253 through 284 )DJ253 - 284250 - 281
1212chain 'D' and (resid 285 through 398 )DJ285 - 398282 - 395
1313chain 'E' and (resid 8 through 42 )EM8 - 421 - 35
1414chain 'E' and (resid 43 through 69 )EM43 - 6936 - 62
1515chain 'E' and (resid 70 through 108 )EM70 - 10863 - 101
1616chain 'E' and (resid 109 through 142 )EM109 - 142102 - 135
1717chain 'E' and (resid 143 through 181 )EM143 - 181136 - 174
1818chain 'E' and (resid 182 through 221 )EM182 - 221175 - 214
1919chain 'E' and (resid 222 through 284 )EM222 - 284215 - 277
2020chain 'E' and (resid 285 through 344 )EM285 - 344278 - 337
2121chain 'E' and (resid 345 through 398 )EM345 - 398338 - 391
2222chain 'F' and (resid 5 through 181 )FP5 - 1811 - 177
2323chain 'F' and (resid 182 through 398 )FP182 - 398178 - 389

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