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- PDB-8xk2: A neutralizing nanobody VHH60 against wt SARS-CoV-2 -

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Basic information

Entry
Database: PDB / ID: 8xk2
TitleA neutralizing nanobody VHH60 against wt SARS-CoV-2
Components
  • Spike protein S1
  • VHH60 nanobody
KeywordsANTIVIRAL PROTEIN/IMMUNE SYSTEM / COVID-19 / spike glycoprotein / virus / antibody / VIRAL PROTEIN/IMMUNE SYSTEM / VIRAL PROTEIN-IMMUNE SYSTEM complex / ANTIVIRAL PROTEIN / ANTIVIRAL PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / host cell endoplasmic reticulum-Golgi intermediate compartment membrane ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / entry receptor-mediated virion attachment to host cell / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / identical protein binding / membrane / plasma membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like ...Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal
Similarity search - Domain/homology
Biological speciesSevere acute respiratory syndrome coronavirus 2
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.4 Å
AuthorsLu, Y. / Guo, H. / Ji, X. / Yang, H.
Funding support2items
OrganizationGrant numberCountry
Other government92169109
Other governmentZD2021CY001
CitationJournal: Cell Death Dis / Year: 2024
Title: A broad neutralizing nanobody against SARS-CoV-2 engineered from an approved drug.
Authors: Qianyun Liu / Yuchi Lu / Chenguang Cai / Yanyan Huang / Li Zhou / Yanbin Guan / Shiying Fu / Youyou Lin / Huan Yan / Zhen Zhang / Xiang Li / Xiuna Yang / Haitao Yang / Hangtian Guo / Ke Lan ...Authors: Qianyun Liu / Yuchi Lu / Chenguang Cai / Yanyan Huang / Li Zhou / Yanbin Guan / Shiying Fu / Youyou Lin / Huan Yan / Zhen Zhang / Xiang Li / Xiuna Yang / Haitao Yang / Hangtian Guo / Ke Lan / Yu Chen / Shin-Chen Hou / Yi Xiong /
Abstract: SARS-CoV-2 infection is initiated by Spike glycoprotein binding to the human angiotensin-converting enzyme 2 (ACE2) receptor via its receptor binding domain. Blocking this interaction has been proven ...SARS-CoV-2 infection is initiated by Spike glycoprotein binding to the human angiotensin-converting enzyme 2 (ACE2) receptor via its receptor binding domain. Blocking this interaction has been proven to be an effective approach to inhibit virus infection. Here we report the discovery of a neutralizing nanobody named VHH60, which was directly produced from an engineering nanobody library based on a commercialized nanobody within a very short period. VHH60 competes with human ACE2 to bind the receptor binding domain of the Spike protein at S, Sand S as determined by structural analysis, with an affinity of 2.56 nM. It inhibits infections of both ancestral SARS-CoV-2 strain and pseudotyped viruses harboring SARS-CoV-2 wildtype, key mutations or variants at the nanomolar level. Furthermore, VHH60 suppressed SARS-CoV-2 infection and propagation 50-fold better and protected mice from death for twice as long as the control group after SARS-CoV-2 nasal infections in vivo. Therefore, VHH60 is not only a powerful nanobody with a promising profile for disease control but also provides evidence for a highly effective and rapid approach to generating therapeutic nanobodies.
History
DepositionDec 22, 2023Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jul 10, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Spike protein S1
B: VHH60 nanobody
C: Spike protein S1
D: VHH60 nanobody
E: Spike protein S1
F: VHH60 nanobody
G: Spike protein S1
H: VHH60 nanobody


Theoretical massNumber of molelcules
Total (without water)152,4038
Polymers152,4038
Non-polymers00
Water00
1
A: Spike protein S1
B: VHH60 nanobody


Theoretical massNumber of molelcules
Total (without water)38,1012
Polymers38,1012
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: Spike protein S1
D: VHH60 nanobody


Theoretical massNumber of molelcules
Total (without water)38,1012
Polymers38,1012
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
E: Spike protein S1
F: VHH60 nanobody


Theoretical massNumber of molelcules
Total (without water)38,1012
Polymers38,1012
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
G: Spike protein S1
H: VHH60 nanobody


Theoretical massNumber of molelcules
Total (without water)38,1012
Polymers38,1012
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)80.850, 100.610, 227.430
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number17
Space group name H-MP2221
Symmetry operation#1: x,y,z
#2: x,-y,-z
#3: -x,y,-z+1/2
#4: -x,-y,z+1/2

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Components

#1: Protein
Spike protein S1


Mass: 24689.797 Da / Num. of mol.: 4 / Fragment: receptor binding domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: S, 2 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P0DTC2
#2: Antibody
VHH60 nanobody


Mass: 13410.872 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli)

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.03 Å3/Da / Density % sol: 59.47 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop
Details: 1.0 M Sodium acetate trihydrate, 0.1 M Sodium HEPES 7.5

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.97853 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 3, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97853 Å / Relative weight: 1
ReflectionResolution: 3.4→31.21 Å / Num. obs: 26210 / % possible obs: 99.84 % / Redundancy: 12.66 % / CC1/2: 0.988 / CC star: 0.997 / Rmerge(I) obs: 0.09947 / Rpim(I) all: 0.09947 / Rrim(I) all: 0.1407 / Net I/σ(I): 6.56
Reflection shell
Resolution (Å)Rmerge(I) obsNum. unique obsCC1/2Diffraction-IDRrim(I) all
3.4-3.61.39541640.8311
3.6-3.850.94539470.91710.983
3.85-4.150.58236790.96810.606
4.15-4.550.37533930.98610.391
4.55-5.080.32831070.98810.342
5.08-5.850.34727530.9810.362
5.85-7.140.32123590.98610.335
7.14-100.18418830.99310.192

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Processing

Software
NameVersionClassification
PHENIX1.20.1-4487refinement
autoPXdata reduction
XDSdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7VNB/tFold model

7vnb


Resolution: 3.4→31.21 Å / SU ML: 0.52 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 31.05 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.3 1998 7.62 %
Rwork0.2557 --
obs0.2592 26210 99.84 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.4→31.21 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9406 0 0 0 9406
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0039643
X-RAY DIFFRACTIONf_angle_d0.5613085
X-RAY DIFFRACTIONf_dihedral_angle_d4.0871350
X-RAY DIFFRACTIONf_chiral_restr0.0441368
X-RAY DIFFRACTIONf_plane_restr0.0061723
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.4-3.480.37011390.34771684X-RAY DIFFRACTION100
3.48-3.580.3571410.30581712X-RAY DIFFRACTION100
3.58-3.680.3241390.29661696X-RAY DIFFRACTION100
3.68-3.80.3331400.28751694X-RAY DIFFRACTION100
3.8-3.940.3331400.28161700X-RAY DIFFRACTION100
3.94-4.10.29171410.25711713X-RAY DIFFRACTION100
4.1-4.280.28111430.23431719X-RAY DIFFRACTION100
4.28-4.510.25451420.21221724X-RAY DIFFRACTION100
4.51-4.790.25731430.22291724X-RAY DIFFRACTION100
4.79-5.160.27211420.21751723X-RAY DIFFRACTION100
5.16-5.670.30231430.24181730X-RAY DIFFRACTION100
5.67-6.490.3071440.25261754X-RAY DIFFRACTION100
6.49-8.150.33721470.2661783X-RAY DIFFRACTION100
8.15-31.210.27721540.25331856X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.44042.5147-0.09228.1327-0.1637-0.0217-0.13220.52640.69591.00310.3723-0.10080.56070.2148-0.32980.7403-0.0080.1430.5614-0.00640.5108-19.1494-23.3736-20.9777
25.88515.8082-0.15286.4821-1.06256.04210.93560.38231.67011.6235-0.74060.90940.19150.1174-0.34970.94430.07790.2220.7106-0.13780.7756-21.8824-28.2238-7.8357
37.1215-2.1198-4.57063.4472-0.60976.76870.76030.19121.59930.61391.4742-0.0557-1.20481.18430.08510.5184-0.10370.02471.0431-0.12780.7759-7.7185-25.545-9.4356
43.5045-1.18480.83462.72090.69621.2001-0.1148-0.3304-0.1492-0.4828-0.0120.0286-0.12870.03280.06890.6442-0.03640.04560.56350.00840.4426-23.9572-39.3026-21.8159
50.92730.29340.55373.92370.77972.47430.08880.0969-0.16840.2591-0.02780.0230.57360.05790.04980.47660.0991-0.00910.5914-0.01370.5504-26.6373-41.4554-26.9387
65.0366-1.05950.47921.90870.6383.47810.0884-0.30650.238-0.0737-0.1346-0.2868-0.71590.07110.07070.7543-0.0786-0.03310.5580.07180.4462-33.0435-35.256-47.5052
70.05070.1004-0.04760.1268-0.05170.08010.31121.03070.83250.06330.66-1.39250.86731.4977-0.79360.61930.1506-0.16950.9227-0.10310.8983-18.7411-36.1375-47.8534
86.6312.2889-3.05095.3678-1.24099.07751.4414-0.2831-0.0061-0.1984-1.56460.442-1.1346-0.6570.0280.70190.0219-0.04010.8146-0.18080.4922-32.6309-27.7389-46.2983
93.08581.835-0.78274.2504-2.07835.7899-0.0613-0.65-0.0757-0.0276-0.0859-0.2524-0.06730.53390.27210.43540.1185-0.12670.4775-0.04710.5429-32.8467-39.4162-49.535
106.39255.8659-1.00166.6653-0.02331.1281-0.60760.5757-0.5769-0.24250.4862-0.55640.2745-0.33550.00370.518-0.0327-0.00940.61970.00120.5329-67.1494-78.4643-23.483
112.36321.77362.71581.37711.16416.16460.6572-0.3922-0.96812.06250.2635-0.5320.3396-1.2069-0.78121.0040.0269-0.29670.72860.14920.9328-70.7497-74.3119-11.1557
121.9579-0.3377-1.25581.8568-0.62330.4856-0.0855-0.0983-0.15330.14770.078-0.1395-0.0811-0.1319-0.01720.5158-0.0132-0.03010.60180.00510.4848-60.1585-61.1759-27.3472
132.71450.43033.70353.41940.30274.93640.96330.935-0.32550.23-0.43290.55342.55540.851-0.18980.9910.14850.07720.91340.04110.477-52.373-69.3902-58.7889
146.5001-4.63443.28395.3408-3.62866.036-0.18840.4251.1554-0.5341-0.6338-1.03480.69240.62680.49810.9216-0.0035-0.01380.7832-0.01840.5695-50.6868-61.8098-48.6716
153.9094-1.121-4.60547.30451.33235.38230.0676-0.46890.0330.36240.3492-0.09090.3702-0.3336-0.28760.67520.0016-0.07150.80210.09190.6444-60.5426-68.1021-46.4041
161.47030.03692.69495.356-1.78078.70550.3210.0588-0.2827-0.5281-0.0159-0.15990.07220.2863-0.20580.7310.01360.10660.72240.08960.5394-52.8644-64.5023-51.9494
171.6861-0.2421.80673.19841.0243.9129-0.26310.3953-0.7212-0.83220.4691-0.3747-0.23910.4089-0.42150.68370.0520.17320.51090.14850.8907-61.3758-6.7148-16.3398
182.6916-2.57542.64636.67911.11555.6244-0.2284-0.4458-0.2768-0.0871.13130.6848-1.1457-1.18720.0770.98750.21920.17960.66940.130.9587-70.7595-0.3024-17.2259
196.0522.2611-1.68272.76771.59083.3457-0.07250.65440.5416-1.83270.2422-0.2426-0.36850.4343-0.30940.53460.05840.11140.86320.38361.3869-62.45432.0666-28.3699
205.1436-0.65250.01334.1219-0.3683.91080.71691.02680.6758-0.8946-0.4332-0.0265-0.86040.1131-0.28690.55630.04160.1110.80320.20160.7795-62.5356-12.1656-29.2259
213.5382-0.27890.87763.2295-0.81851.50810.50020.465-0.4102-0.3424-0.1794-0.0975-0.2837-0.3195-0.29090.75030.078-0.09420.7765-0.10470.6099-58.8339-23.5257-22.3349
227.4516-1.4891-2.51652.8195-0.5737.2404-0.87980.0377-0.7347-1.35-0.11410.67781.52360.41590.84590.75360.26670.02810.9753-0.0390.6162-56.7581-35.9398-21.8664
237.5199-1.976-0.30543.655-0.88762.91960.68520.54470.7987-0.2893-0.462-0.6373-0.40760.6697-0.29860.7232-0.11710.04950.70460.13660.5907-52.0402-11.1796-25.0797
249.36630.6029-3.02525.9721-1.81275.6279-0.44820.4905-1.02150.48190.3971-0.56760.11210.4565-0.10420.90410.0774-0.13350.7931-0.01390.4185-59.2576-35.55423.3816
253.7918-0.0366-0.88955.556-0.31444.89050.35070.21010.04060.1436-0.2609-0.01511.16040.2681-0.10850.36930.06030.01070.52520.01350.369-59.1497-30.9177-9.0719
261.9963-2.6634-1.15994.9906-1.6858.68530.7908-0.4739-1.67210.33190.3419-0.4156-0.0092-2.0194-1.06640.74130.0570.09120.68760.19990.7732-68.8393-29.4364-5.3966
276.80096.24914.6167.65610.81459.03832.2816-0.1943-1.02261.071-1.0818-0.588-0.21730.6179-0.93031.20510.22540.15320.6443-0.01340.7491-57.8662-26.79921.2747
284.45854.2147-4.36255.9688-2.02958.830.2413-0.3283-0.5155-0.0775-0.50720.07260.3337-0.15050.19330.69210.1105-0.12230.61160.0750.7674-59.1335-36.6635-6.3415
295.4356-1.1201-1.06043.6196-0.13322.3416-0.7793-0.1490.0121-0.15480.2344-0.99420.17640.22490.25780.6094-0.1132-0.09930.5080.01710.7045-21.9436-97.5259-17.4218
304.3227-0.32252.30930.0278-0.2161.21530.65851.57850.2451-0.4879-1.10780.20470.08610.2355-0.7580.43680.1713-0.17470.8714-0.16621.0331-17.8967-102.1891-29.9778
313.3061-1.83660.79586.12030.31884.35210.30760.7173-0.6126-0.9696-0.34030.10020.647-0.17350.00640.8297-0.0601-0.05440.5864-0.15810.7717-23.6738-90.3951-30.4758
325.5084-6.3957-3.14117.49114.30649.25330.17271.64610.01522.18380.0950.18991.0215-0.87040.04670.6201-0.0495-0.04880.73960.11460.7314-39.5006-87.978-18.9308
336.4082-1.0681-1.13575.3070.17546.73770.39280.84510.781-0.8670.0351-0.42190.06310.446-0.51110.7050.07660.17270.691-0.04510.6047-20.4608-78.8492-26.9675
341.95490.150.99486.9734-3.20044.8486-0.2844-0.03020.1909-1.3217-0.6224-0.12970.4112-0.13250.7470.60670.0802-0.0540.72560.17210.5257-26.9479-65.3954-25.7273
353.1076-3.05810.44483.34350.79064.7081-0.39620.4092-0.034-0.350.54380.27620.0226-0.47620.11311.0381-0.1866-0.2560.7015-0.05850.7834-34.0341-91.1612-26.1465
362.57133.62881.70795.08552.76865.0696-0.17320.042-0.027-0.51090.1390.1501-0.05520.34350.07290.92260.02550.00540.6504-0.10330.6351-25.1121-62.37740.9851
376.89136.67793.45787.65031.57054.2323-0.751-0.0741.5910.3033-0.01610.45430.6248-0.23930.49530.9088-0.096-0.06150.692-0.09450.3648-27.0872-68.26160.2678
382.01452.666-5.02894.75761.17763.92040.29790.10360.55570.7543-0.2844-0.37172.0175-2.02850.20971.07-0.2867-0.15020.7864-0.25280.6702-39.9656-75.459-11.4987
391.60611.8071-0.59032.35290.36993.2166-0.4778-0.22-0.2636-0.3960.67760.1012-0.0801-0.0855-0.06830.6087-0.1578-0.04310.6967-0.04360.5939-25.4161-65.8863-10.1339
401.83961.71991.13182.77513.0974.6693-0.80530.22360.4311-0.20170.99020.1033-1.27440.40530.07080.30530.233-0.09130.70420.13980.4281-21.9878-63.2626-12.4507
414.91113.01842.55551.86361.46981.6654-0.33230.46840.4879-0.9889-0.26220.10370.4665-0.8392-0.3290.4109-0.07490.03040.43260.11710.9678-24.5367-80.3554-12.6781
424.21364.8962-1.49158.3585-2.51820.7575-0.80060.54250.5735-0.74961.1123-0.88590.22240.72270.57580.0135-0.06140.29820.9601-0.14641.2681-13.3073-69.7803-10.9092
433.0045-2.4708-0.13435.17150.58464.68240.2052-0.1759-2.3393-1.2115-0.59170.17070.7931-0.8536-1.1281.092-0.1554-0.21120.6495-0.04640.6419-27.8418-75.6852-3.0921
443.11271.46243.22036.8873-0.58764.07462.172-0.2963-0.8274-0.6227-1.53030.9891.16930.3645-0.01840.7568-0.0594-0.08410.53240.08310.7671-22.5281-70.6641-1.0702
458.4801-5.3924.9618.6579-1.14393.66880.07521.36420.87560.5048-0.9899-1.446-0.32322.11310.74570.9495-0.12070.09940.85550.27210.8599-12.169-60.2144-3.6934
462.5931.22673.06611.28390.74894.3104-0.30520.54390.40860.67380.10710.8253-0.9309-0.07060.12660.72360.13870.05240.6937-0.16860.6466-26.7515-62.0545-7.4727
475.54545.89742.68766.89634.72454.68510.2002-0.3260.211-0.0699-0.08620.0151-0.508-0.3644-0.12710.58760.07190.01160.6205-0.06360.7404-31.5349-65.5965-11.6955
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 332 through 365 )
2X-RAY DIFFRACTION2chain 'A' and (resid 366 through 380 )
3X-RAY DIFFRACTION3chain 'A' and (resid 381 through 394 )
4X-RAY DIFFRACTION4chain 'A' and (resid 395 through 469 )
5X-RAY DIFFRACTION5chain 'A' and (resid 470 through 526 )
6X-RAY DIFFRACTION6chain 'B' and (resid 1 through 59 )
7X-RAY DIFFRACTION7chain 'B' and (resid 60 through 67 )
8X-RAY DIFFRACTION8chain 'B' and (resid 68 through 83 )
9X-RAY DIFFRACTION9chain 'B' and (resid 84 through 121 )
10X-RAY DIFFRACTION10chain 'C' and (resid 332 through 365 )
11X-RAY DIFFRACTION11chain 'C' and (resid 366 through 394 )
12X-RAY DIFFRACTION12chain 'C' and (resid 395 through 525 )
13X-RAY DIFFRACTION13chain 'D' and (resid 2 through 24 )
14X-RAY DIFFRACTION14chain 'D' and (resid 25 through 45 )
15X-RAY DIFFRACTION15chain 'D' and (resid 46 through 73 )
16X-RAY DIFFRACTION16chain 'D' and (resid 74 through 121 )
17X-RAY DIFFRACTION17chain 'E' and (resid 335 through 353 )
18X-RAY DIFFRACTION18chain 'E' and (resid 354 through 365 )
19X-RAY DIFFRACTION19chain 'E' and (resid 366 through 394 )
20X-RAY DIFFRACTION20chain 'E' and (resid 395 through 437 )
21X-RAY DIFFRACTION21chain 'E' and (resid 438 through 479 )
22X-RAY DIFFRACTION22chain 'E' and (resid 480 through 494 )
23X-RAY DIFFRACTION23chain 'E' and (resid 495 through 515 )
24X-RAY DIFFRACTION24chain 'F' and (resid 1 through 25 )
25X-RAY DIFFRACTION25chain 'F' and (resid 26 through 59 )
26X-RAY DIFFRACTION26chain 'F' and (resid 60 through 73 )
27X-RAY DIFFRACTION27chain 'F' and (resid 74 through 83 )
28X-RAY DIFFRACTION28chain 'F' and (resid 84 through 121 )
29X-RAY DIFFRACTION29chain 'G' and (resid 334 through 375 )
30X-RAY DIFFRACTION30chain 'G' and (resid 376 through 395 )
31X-RAY DIFFRACTION31chain 'G' and (resid 396 through 437 )
32X-RAY DIFFRACTION32chain 'G' and (resid 438 through 451 )
33X-RAY DIFFRACTION33chain 'G' and (resid 452 through 469 )
34X-RAY DIFFRACTION34chain 'G' and (resid 470 through 494 )
35X-RAY DIFFRACTION35chain 'G' and (resid 495 through 515 )
36X-RAY DIFFRACTION36chain 'H' and (resid 2 through 16 )
37X-RAY DIFFRACTION37chain 'H' and (resid 17 through 25 )
38X-RAY DIFFRACTION38chain 'H' and (resid 26 through 32 )
39X-RAY DIFFRACTION39chain 'H' and (resid 33 through 39 )
40X-RAY DIFFRACTION40chain 'H' and (resid 40 through 52 )
41X-RAY DIFFRACTION41chain 'H' and (resid 53 through 59 )
42X-RAY DIFFRACTION42chain 'H' and (resid 60 through 67 )
43X-RAY DIFFRACTION43chain 'H' and (resid 68 through 77 )
44X-RAY DIFFRACTION44chain 'H' and (resid 78 through 84 )
45X-RAY DIFFRACTION45chain 'H' and (resid 85 through 91 )
46X-RAY DIFFRACTION46chain 'H' and (resid 92 through 98 )
47X-RAY DIFFRACTION47chain 'H' and (resid 99 through 121 )

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