+Open data
-Basic information
Entry | Database: PDB / ID: 8x7w | ||||||
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Title | Structure of dimeric human SCMC complex | ||||||
Components |
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Keywords | CYTOSOLIC PROTEIN / oocyte / subcortical / complex | ||||||
Function / homology | Function and homology information embryonic process involved in female pregnancy / subcortical maternal complex / regulation of localization / establishment of organelle localization / endoplasmic reticulum localization / cortical granule exocytosis / establishment or maintenance of apical/basal cell polarity / SUMO is conjugated to E1 (UBA2:SAE1) / cortical granule / SUMOylation of nuclear envelope proteins ...embryonic process involved in female pregnancy / subcortical maternal complex / regulation of localization / establishment of organelle localization / endoplasmic reticulum localization / cortical granule exocytosis / establishment or maintenance of apical/basal cell polarity / SUMO is conjugated to E1 (UBA2:SAE1) / cortical granule / SUMOylation of nuclear envelope proteins / SUMO is transferred from E1 to E2 (UBE2I, UBC9) / positive regulation of meiotic nuclear division / SUMO is proteolytically processed / positive regulation of embryonic development / SUMOylation of transcription factors / Postmitotic nuclear pore complex (NPC) reformation / SUMOylation of transcription cofactors / regulation of establishment of protein localization / establishment of spindle localization / septin ring / mitochondrion localization / SUMOylation of DNA damage response and repair proteins / SUMOylation of DNA replication proteins / embryonic pattern specification / SUMOylation of SUMOylation proteins / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / SUMOylation of RNA binding proteins / SUMOylation of chromatin organization proteins / positive regulation of double-strand break repair / detection of maltose stimulus / replication fork processing / maltose transport complex / regulation of cell division / ubiquitin-like protein ligase binding / exocytosis / carbohydrate transport / protein sumoylation / positive regulation of double-strand break repair via homologous recombination / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / tubulin binding / ATP-binding cassette (ABC) transporter complex / cell chemotaxis / actin filament organization / condensed nuclear chromosome / negative regulation of canonical Wnt signaling pathway / protein tag activity / transcription corepressor activity / regulation of protein localization / outer membrane-bounded periplasmic space / cell cortex / regulation of inflammatory response / transcription regulator complex / periplasmic space / intracellular membrane-bounded organelle / DNA damage response / nucleolus / Golgi apparatus / negative regulation of transcription by RNA polymerase II / protein-containing complex / mitochondrion / RNA binding / ATP binding / identical protein binding / membrane / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Escherichia coli K-12 (bacteria) Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.36 Å | ||||||
Authors | Chi, P. / Ou, G. / Liu, S. / Lu, Y. / Li, J. / Li, J. / Wang, X. / Deng, D. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: Structure of dimeric human SCMC complex Authors: Chi, P. / Ou, G. / Liu, S. / Lu, Y. / Deng, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8x7w.cif.gz | 531.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8x7w.ent.gz | 408.8 KB | Display | PDB format |
PDBx/mmJSON format | 8x7w.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8x7w_validation.pdf.gz | 1.3 MB | Display | wwPDB validaton report |
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Full document | 8x7w_full_validation.pdf.gz | 1.3 MB | Display | |
Data in XML | 8x7w_validation.xml.gz | 87 KB | Display | |
Data in CIF | 8x7w_validation.cif.gz | 131.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/x7/8x7w ftp://data.pdbj.org/pub/pdb/validation_reports/x7/8x7w | HTTPS FTP |
-Related structure data
Related structure data | 38129MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 171393.734 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K-12 (bacteria), (gene. exp.) Homo sapiens (human) Strain: K-12 / Gene: malE, NLRP5 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: P0AEX9, UniProt: P59047 #2: Protein | Mass: 18479.176 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: OOEP / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: A6NGQ2 #3: Protein | Mass: 59899.367 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K-12 (bacteria), (gene. exp.) Homo sapiens (human) Strain: K-12 / Gene: SMT3, TLE6 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q12306, UniProt: Q9H808 Has protein modification | N | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: human SCMC complex / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1800 nm / Nominal defocus min: 1100 nm |
Image recording | Electron dose: 51.336 e/Å2 / Film or detector model: GATAN K2 IS (4k x 4k) |
-Processing
CTF correction | Type: NONE | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 3.36 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 163605 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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