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Open data
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Basic information
Entry | Database: PDB / ID: 8wwy | ||||||
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Title | Crystal structure of mouse TIFA/TIFAB heterodimer | ||||||
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![]() | SIGNALING PROTEIN / Complex | ||||||
Function / homology | ![]() : / regulation of muscle organ development / learned vocalization behavior / negative regulation of saliva secretion / hard palate morphogenesis / negative regulation of relaxation of muscle / trigeminal nerve development / regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator / craniofacial suture morphogenesis / auditory behavior ...: / regulation of muscle organ development / learned vocalization behavior / negative regulation of saliva secretion / hard palate morphogenesis / negative regulation of relaxation of muscle / trigeminal nerve development / regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator / craniofacial suture morphogenesis / auditory behavior / thorax and anterior abdomen determination / vestibulocochlear nerve formation / Alpha-protein kinase 1 signaling pathway / cochlea morphogenesis / genitalia morphogenesis / TAK1-dependent IKK and NF-kappa-B activation / mastication / genitalia development / peristalsis / myeloid cell differentiation / deubiquitinase activator activity / inner ear morphogenesis / cytoplasmic pattern recognition receptor signaling pathway / cochlea development / inner ear development / neuromuscular process controlling balance / hematopoietic progenitor cell differentiation / canonical NF-kappaB signal transduction / lipopolysaccharide-mediated signaling pathway / tumor necrosis factor-mediated signaling pathway / protein homooligomerization / regulation of gene expression / positive regulation of canonical NF-kappaB signal transduction / innate immune response / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Nakamura, T. | ||||||
Funding support | ![]()
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![]() | ![]() Title: TIFAB regulates the TIFA-TRAF6 signaling pathway involved in innate immunity by forming a heterodimer complex with TIFA. Authors: Nakamura, T. / Ohyama, C. / Sakamoto, M. / Toma, T. / Tateishi, H. / Matsuo, M. / Chirifu, M. / Ikemizu, S. / Morioka, H. / Fujita, M. / Inoue, J.I. / Yamagata, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 299.7 KB | Display | ![]() |
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PDB format | ![]() | 202.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 17577.070 Da / Num. of mol.: 2 / Mutation: C36S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 16167.834 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Chemical | ChemComp-HX2 / ( #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 50.9 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: HEPES, PEG 2000, Hexanediol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 15, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.79→47.04 Å / Num. obs: 65696 / % possible obs: 99.7 % / Redundancy: 19.5 % / Biso Wilson estimate: 31.4 Å2 / CC1/2: 1 / Net I/σ(I): 21.4 |
Reflection shell | Resolution: 1.79→1.9 Å / Num. unique obs: 10329 / CC1/2: 0.738 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 40.83 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.79→47.04 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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