+Open data
-Basic information
Entry | Database: PDB / ID: 8wu1 | ||||||
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Title | Cryo-EM structure of CB1-beta-arrestin1 complex | ||||||
Components |
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Keywords | MEMBRANE PROTEIN / cannabinoid receptor / arrestin / biased signal / class A GPCR | ||||||
Function / homology | Function and homology information renal water retention / Defective AVP does not bind AVPR2 and causes neurohypophyseal diabetes insipidus (NDI) / MAP2K and MAPK activation / Vasopressin-like receptors / cannabinoid signaling pathway / regulation of penile erection / regulation of systemic arterial blood pressure by vasopressin / vasopressin receptor activity / Activation of SMO / retrograde trans-synaptic signaling by endocannabinoid ...renal water retention / Defective AVP does not bind AVPR2 and causes neurohypophyseal diabetes insipidus (NDI) / MAP2K and MAPK activation / Vasopressin-like receptors / cannabinoid signaling pathway / regulation of penile erection / regulation of systemic arterial blood pressure by vasopressin / vasopressin receptor activity / Activation of SMO / retrograde trans-synaptic signaling by endocannabinoid / Golgi Associated Vesicle Biogenesis / cannabinoid receptor activity / Lysosome Vesicle Biogenesis / negative regulation of mast cell activation / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / AP-2 adaptor complex binding / negative regulation of fatty acid beta-oxidation / clathrin heavy chain binding / negative regulation of dopamine secretion / Ub-specific processing proteases / positive regulation of acute inflammatory response to antigenic stimulus / clathrin coat of coated pit / negative regulation of serotonin secretion / regulation of feeding behavior / hemostasis / regulation of presynaptic cytosolic calcium ion concentration / desensitization of G protein-coupled receptor signaling pathway / positive regulation of systemic arterial blood pressure / telencephalon development / Cargo recognition for clathrin-mediated endocytosis / negative regulation of action potential / inositol hexakisphosphate binding / Class A/1 (Rhodopsin-like receptors) / Clathrin-mediated endocytosis / clathrin-dependent endocytosis / positive regulation of blood pressure / positive regulation of fever generation / G protein-coupled receptor internalization / regulation of metabolic process / acetylcholine receptor binding / axonal fasciculation / Thrombin signalling through proteinase activated receptors (PARs) / positive regulation of intracellular signal transduction / G alpha (s) signalling events / regulation of synaptic transmission, GABAergic / clathrin binding / negative regulation of Notch signaling pathway / small molecule binding / pseudopodium / phosphatidylinositol-3,4,5-trisphosphate binding / regulation of insulin secretion / positive regulation of receptor internalization / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / endocytic vesicle / GABA-ergic synapse / maternal process involved in female pregnancy / activation of adenylate cyclase activity / positive regulation of vasoconstriction / cellular response to hormone stimulus / regulation of synaptic transmission, glutamatergic / visual perception / negative regulation of blood pressure / response to nutrient / response to cocaine / response to cytokine / G protein-coupled receptor binding / G protein-coupled receptor activity / peptide binding / clathrin-coated endocytic vesicle membrane / response to nicotine / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / adenylate cyclase-activating G protein-coupled receptor signaling pathway / receptor internalization / memory / positive regulation of neuron projection development / Vasopressin regulates renal water homeostasis via Aquaporins / actin cytoskeleton / protein transport / Cargo recognition for clathrin-mediated endocytosis / Clathrin-mediated endocytosis / glucose homeostasis / presynaptic membrane / G alpha (i) signalling events / growth cone / cytoplasmic vesicle / ubiquitin-dependent protein catabolic process / G alpha (s) signalling events / spermatogenesis / response to ethanol / mitochondrial outer membrane / response to lipopolysaccharide / molecular adaptor activity / positive regulation of ERK1 and ERK2 cascade / endosome / positive regulation of protein phosphorylation / positive regulation of apoptotic process / G protein-coupled receptor signaling pathway / membrane raft / negative regulation of cell population proliferation / glutamatergic synapse Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | ||||||
Authors | Liao, Y. / Zhang, H. / Shen, Q. / Cai, C. | ||||||
Funding support | China, 1items
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Citation | Journal: to be published Title: Cryo-EM structure of CB1-beta-arrestin1 complex Authors: Liao, Y. / Zhang, H. / Shen, Q. / Cai, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8wu1.cif.gz | 185.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8wu1.ent.gz | 137.7 KB | Display | PDB format |
PDBx/mmJSON format | 8wu1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wu/8wu1 ftp://data.pdbj.org/pub/pdb/validation_reports/wu/8wu1 | HTTPS FTP |
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-Related structure data
Related structure data | 37849MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 44135.273 Da / Num. of mol.: 1 / Mutation: R169E, I386A, V387A, F388A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Gene: ARRB1 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P17870 |
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#2: Antibody | Mass: 25650.502 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli BL21(DE3) (bacteria) |
#3: Antibody | Mass: 23435.064 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli BL21(DE3) (bacteria) |
#4: Protein | Mass: 50310.129 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CNR1, AVPR2 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P21554, UniProt: P30518 |
#5: Chemical | ChemComp-KCA / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Cryo-EM structure of human cannabinoid receptor 1 with effector protein Type: COMPLEX Details: MDMB-Fubinaca, cannabinoid receptor 1, arrestin2, Fab30 Entity ID: #1-#4 / Source: RECOMBINANT | ||||||||||||
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Source (natural) |
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Source (recombinant) |
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Buffer solution | pH: 7.5 | ||||||||||||
Specimen | Conc.: 5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 5000 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 52 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 110196 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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