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- PDB-8wo5: Cryo-EM structure of the intact flagellar motor-hook complex in t... -

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Basic information

Entry
Database: PDB / ID: 8wo5
TitleCryo-EM structure of the intact flagellar motor-hook complex in the CCW state
Components
  • (Flagellar basal-body rod protein ...) x 3
  • (Flagellar biosynthetic protein ...) x 3
  • (Flagellar motor switch protein ...) x 3
  • Flagellar L-ring protein
  • Flagellar M-ring protein
  • Flagellar P-ring protein
  • Flagellar basal body rod protein FlgB
  • Flagellar hook protein FlgE
  • Flagellar hook-basal body complex protein FliE
KeywordsMOTOR PROTEIN / Flagellum / Flagellar motor / C ring / Switch complex
Function / homology
Function and homology information


bacterial-type flagellum basal body, distal rod, L ring / bacterial-type flagellum basal body, distal rod, P ring / bacterial-type flagellum basal body, distal rod / bacterial-type flagellum basal body, rod / bacterial-type flagellum organization / bacterial-type flagellum basal body, MS ring / bacterial-type flagellum basal body / bacterial-type flagellum-dependent swarming motility / positive chemotaxis / bacterial-type flagellum assembly ...bacterial-type flagellum basal body, distal rod, L ring / bacterial-type flagellum basal body, distal rod, P ring / bacterial-type flagellum basal body, distal rod / bacterial-type flagellum basal body, rod / bacterial-type flagellum organization / bacterial-type flagellum basal body, MS ring / bacterial-type flagellum basal body / bacterial-type flagellum-dependent swarming motility / positive chemotaxis / bacterial-type flagellum assembly / cytoskeletal motor activity / protein secretion / bacterial-type flagellum-dependent cell motility / protein targeting / cell outer membrane / chemotaxis / outer membrane-bounded periplasmic space / structural molecule activity / plasma membrane
Similarity search - Function
Flagellar L-ring protein / Flagellar L-ring protein / Flagellar P-ring protein / Flagellar P-ring protein / Flagellar basal-body rod protein FlgC / Flagellar basal-body rod FlgG / Flagellar motor switch protein FliN, N-terminal / : / Flagellar motor switch protein FliN N-terminal / Flagellar hook-basal body complex protein FliE ...Flagellar L-ring protein / Flagellar L-ring protein / Flagellar P-ring protein / Flagellar P-ring protein / Flagellar basal-body rod protein FlgC / Flagellar basal-body rod FlgG / Flagellar motor switch protein FliN, N-terminal / : / Flagellar motor switch protein FliN N-terminal / Flagellar hook-basal body complex protein FliE / Flagellar basal-body rod protein FlgB / Flagellar hook-basal body complex protein FliE / Flagellar motor switch FliN / Flagellar motor switch FliN/Type III secretion HrcQb / Flagellar biosynthesis protein FliQ / Flagellar biosynthesis protein FliR / Flagellar transport protein FliP / Flagellar motor switch protein FliM / Flagellar motor switch protein FliM / Flagellar hook protein FlgE superfamily / Flagellar hook protein FlgE / Flagellar basal body protein FlaE D2 domain / Flagellar motor switch protein FliG / Flagellar motor switch protein FliG, alpha-helical / Flagellar motor switch protein FliG, C-terminal / Flagellar motor switch protein FliG, N-terminal domain / Flagellar motor switch protein FliG, middle domain / FliG C-terminal domain / FliG middle domain / FliG N-terminal domain / : / Flagellar hook protein FlgE/F/G D1 domain / CheC-like superfamily / Flagellar hook-basal body protein, FlgE/F/G / Flagellar hook-basal body protein, FlgE/F/G-like / Flagellar motor switch protein FliN-like, C-terminal domain / Type III secretion system inner membrane R protein / Bacterial export protein family 3 / SpoA-like superfamily / Type III flagellar switch regulator (C-ring) FliN C-term / Bacterial export proteins, family 1 / Bacterial export proteins, family 3 / Flagella transport protein fliP family signature 1. / Type III secretion system inner membrane P protein / FliP family / Flagella transport protein fliP family signature 2. / Flagellar basal body rod protein, conserved site / Flagella basal body rod proteins signature. / Flagellar basal body rod protein, N-terminal / Flagellar basal-body/hook protein, C-terminal domain / Flagella basal body rod protein / Flagellar basal body rod FlgEFG protein C-terminal / Flagellar M-ring protein FliF / Flagellar M-ring C-terminal / Flagellar M-ring protein C-terminal / Lipoprotein YscJ/Flagellar M-ring protein / Secretory protein of YscJ/FliF family / Flagellar M-ring , N-terminal / AMP-binding enzyme, C-terminal domain superfamily / Prokaryotic membrane lipoprotein lipid attachment site profile.
Similarity search - Domain/homology
Flagellar basal-body rod protein FlgC / Flagellar hook protein FlgE / Flagellar basal-body rod protein FlgG / Flagellar motor switch protein FliG / Flagellar biosynthetic protein FliQ / Flagellar L-ring protein / Flagellar M-ring protein / Flagellar P-ring protein / Flagellar basal-body rod protein FlgF / Flagellar basal body rod protein FlgB ...Flagellar basal-body rod protein FlgC / Flagellar hook protein FlgE / Flagellar basal-body rod protein FlgG / Flagellar motor switch protein FliG / Flagellar biosynthetic protein FliQ / Flagellar L-ring protein / Flagellar M-ring protein / Flagellar P-ring protein / Flagellar basal-body rod protein FlgF / Flagellar basal body rod protein FlgB / Flagellar motor switch protein FliM / Flagellar motor switch protein FliN / Flagellar hook-basal body complex protein FliE / Flagellar biosynthetic protein FliP / Flagellar biosynthetic protein FliR
Similarity search - Component
Biological speciesSalmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 7.4 Å
AuthorsTan, J.X. / Zhang, L. / Zhou, Y. / Zhu, Y.Q.
Funding support China, 3items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)81925024 China
National Natural Science Foundation of China (NSFC)U23A20163 China
Ministry of Science and Technology (MoST, China)2017YFA0503900 China
CitationJournal: To Be Published
Title: Cryo-EM structure of the intact flagellar motor-hook complex in the CCW state
Authors: Tan, J.X. / Zhang, L. / Zhou, Y. / Zhu, Y.Q.
History
DepositionOct 6, 2023Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Sep 4, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
0: Flagellar basal-body rod protein FlgG
1: Flagellar basal-body rod protein FlgG
2: Flagellar basal-body rod protein FlgG
3: Flagellar basal-body rod protein FlgG
4: Flagellar basal-body rod protein FlgG
5: Flagellar basal-body rod protein FlgG
6: Flagellar basal-body rod protein FlgG
7: Flagellar basal-body rod protein FlgG
8: Flagellar basal-body rod protein FlgG
9: Flagellar basal-body rod protein FlgG
A: Flagellar L-ring protein
A0: Flagellar basal body rod protein FlgB
A1: Flagellar hook-basal body complex protein FliE
A2: Flagellar hook-basal body complex protein FliE
A3: Flagellar hook-basal body complex protein FliE
A4: Flagellar hook-basal body complex protein FliE
A5: Flagellar hook-basal body complex protein FliE
A6: Flagellar basal body rod protein FlgB
A7: Flagellar basal body rod protein FlgB
A8: Flagellar basal body rod protein FlgB
A9: Flagellar basal body rod protein FlgB
AA: Flagellar basal-body rod protein FlgF
AB: Flagellar basal-body rod protein FlgF
AC: Flagellar basal-body rod protein FlgF
AD: Flagellar basal-body rod protein FlgF
AE: Flagellar basal-body rod protein FlgF
AF: Flagellar basal-body rod protein FlgG
AG: Flagellar basal-body rod protein FlgG
AH: Flagellar basal-body rod protein FlgG
AI: Flagellar basal-body rod protein FlgG
AJ: Flagellar basal-body rod protein FlgG
AK: Flagellar basal-body rod protein FlgG
AL: Flagellar basal-body rod protein FlgG
AM: Flagellar basal-body rod protein FlgG
AN: Flagellar basal-body rod protein FlgG
AO: Flagellar M-ring protein
AP: Flagellar M-ring protein
AQ: Flagellar M-ring protein
AR: Flagellar M-ring protein
AS: Flagellar M-ring protein
AT: Flagellar M-ring protein
AU: Flagellar M-ring protein
AV: Flagellar M-ring protein
AW: Flagellar M-ring protein
AX: Flagellar M-ring protein
AY: Flagellar M-ring protein
AZ: Flagellar M-ring protein
Aa: Flagellar M-ring protein
Ab: Flagellar biosynthetic protein FliQ
Ac: Flagellar M-ring protein
Ad: Flagellar M-ring protein
Ae: Flagellar M-ring protein
Af: Flagellar M-ring protein
Ag: Flagellar M-ring protein
Ah: Flagellar M-ring protein
Ai: Flagellar M-ring protein
Aj: Flagellar M-ring protein
Ak: Flagellar M-ring protein
Al: Flagellar M-ring protein
Am: Flagellar M-ring protein
An: Flagellar M-ring protein
Ao: Flagellar M-ring protein
Ap: Flagellar M-ring protein
Aq: Flagellar biosynthetic protein FliQ
Ar: Flagellar biosynthetic protein FliQ
As: Flagellar biosynthetic protein FliQ
At: Flagellar biosynthetic protein FliR
Au: Flagellar biosynthetic protein FliP
Av: Flagellar biosynthetic protein FliP
Aw: Flagellar biosynthetic protein FliP
Ax: Flagellar biosynthetic protein FliP
Ay: Flagellar biosynthetic protein FliP
Az: Flagellar hook-basal body complex protein FliE
B: Flagellar L-ring protein
BA: Flagellar basal-body rod protein FlgC
BB: Flagellar basal-body rod protein FlgC
BC: Flagellar basal-body rod protein FlgC
BD: Flagellar basal-body rod protein FlgC
BE: Flagellar basal-body rod protein FlgC
BF: Flagellar basal-body rod protein FlgC
BG: Flagellar M-ring protein
BH: Flagellar M-ring protein
BI: Flagellar M-ring protein
BJ: Flagellar M-ring protein
BK: Flagellar M-ring protein
BL: Flagellar M-ring protein
BM: Flagellar M-ring protein
BN: Flagellar M-ring protein
BO: Flagellar M-ring protein
BP: Flagellar M-ring protein
BQ: Flagellar M-ring protein
BR: Flagellar M-ring protein
BS: Flagellar M-ring protein
BT: Flagellar M-ring protein
BU: Flagellar M-ring protein
BV: Flagellar M-ring protein
BW: Flagellar M-ring protein
BX: Flagellar M-ring protein
C: Flagellar L-ring protein
D: Flagellar L-ring protein
E: Flagellar L-ring protein
F: Flagellar L-ring protein
G: Flagellar L-ring protein
H: Flagellar L-ring protein
I: Flagellar L-ring protein
J: Flagellar L-ring protein
K: Flagellar L-ring protein
L: Flagellar L-ring protein
M: Flagellar L-ring protein
N: Flagellar L-ring protein
O: Flagellar L-ring protein
P: Flagellar L-ring protein
Q: Flagellar L-ring protein
R: Flagellar L-ring protein
S: Flagellar L-ring protein
T: Flagellar L-ring protein
U: Flagellar L-ring protein
UI: Flagellar M-ring protein
UJ: Flagellar M-ring protein
UK: Flagellar M-ring protein
UL: Flagellar M-ring protein
UM: Flagellar M-ring protein
UN: Flagellar M-ring protein
UO: Flagellar M-ring protein
UP: Flagellar M-ring protein
V: Flagellar L-ring protein
W: Flagellar L-ring protein
WA: Flagellar M-ring protein
WB: Flagellar M-ring protein
WC: Flagellar M-ring protein
WD: Flagellar M-ring protein
WE: Flagellar M-ring protein
WF: Flagellar M-ring protein
WG: Flagellar M-ring protein
WH: Flagellar M-ring protein
WI: Flagellar M-ring protein
WJ: Flagellar M-ring protein
WK: Flagellar M-ring protein
WL: Flagellar M-ring protein
WM: Flagellar M-ring protein
WN: Flagellar M-ring protein
WO: Flagellar M-ring protein
WP: Flagellar M-ring protein
WQ: Flagellar M-ring protein
WR: Flagellar M-ring protein
WS: Flagellar M-ring protein
WT: Flagellar M-ring protein
WU: Flagellar M-ring protein
WV: Flagellar M-ring protein
WW: Flagellar M-ring protein
X: Flagellar L-ring protein
Y: Flagellar L-ring protein
Z: Flagellar L-ring protein
ZA: Flagellar basal-body rod protein FlgG
ZB: Flagellar basal-body rod protein FlgG
ZC: Flagellar basal-body rod protein FlgG
ZD: Flagellar basal-body rod protein FlgG
ZE: Flagellar basal-body rod protein FlgG
ZF: Flagellar hook protein FlgE
ZG: Flagellar hook protein FlgE
ZH: Flagellar hook protein FlgE
ZI: Flagellar hook protein FlgE
ZJ: Flagellar hook protein FlgE
ZK: Flagellar hook protein FlgE
ZL: Flagellar hook protein FlgE
ZM: Flagellar hook protein FlgE
ZN: Flagellar hook protein FlgE
ZO: Flagellar hook protein FlgE
ZP: Flagellar hook protein FlgE
ZQ: Flagellar hook protein FlgE
ZR: Flagellar hook protein FlgE
ZS: Flagellar hook protein FlgE
ZT: Flagellar hook protein FlgE
ZU: Flagellar hook protein FlgE
ZV: Flagellar hook protein FlgE
ZW: Flagellar hook protein FlgE
ZX: Flagellar hook protein FlgE
ZY: Flagellar hook protein FlgE
ZZ: Flagellar hook protein FlgE
Za: Flagellar hook protein FlgE
Zb: Flagellar hook protein FlgE
Zc: Flagellar hook protein FlgE
Zd: Flagellar hook protein FlgE
Ze: Flagellar hook protein FlgE
Zf: Flagellar hook protein FlgE
Zg: Flagellar hook protein FlgE
Zh: Flagellar hook protein FlgE
a: Flagellar P-ring protein
b: Flagellar P-ring protein
c: Flagellar P-ring protein
d: Flagellar P-ring protein
e: Flagellar P-ring protein
f: Flagellar P-ring protein
g: Flagellar P-ring protein
h: Flagellar P-ring protein
i: Flagellar P-ring protein
j: Flagellar P-ring protein
k: Flagellar P-ring protein
l: Flagellar P-ring protein
m: Flagellar P-ring protein
n: Flagellar P-ring protein
o: Flagellar P-ring protein
p: Flagellar P-ring protein
q: Flagellar P-ring protein
r: Flagellar P-ring protein
s: Flagellar P-ring protein
t: Flagellar P-ring protein
u: Flagellar P-ring protein
v: Flagellar P-ring protein
w: Flagellar P-ring protein
x: Flagellar P-ring protein
y: Flagellar P-ring protein
z: Flagellar P-ring protein
C3: Flagellar M-ring protein
C4: Flagellar motor switch protein FliN
C5: Flagellar motor switch protein FliN
C6: Flagellar motor switch protein FliG
C7: Flagellar M-ring protein
C8: Flagellar motor switch protein FliM
C9: Flagellar motor switch protein FliN
C0: Flagellar motor switch protein FliN
DA: Flagellar motor switch protein FliN
DB: Flagellar motor switch protein FliG
DC: Flagellar motor switch protein FliN
DD: Flagellar motor switch protein FliG
DE: Flagellar M-ring protein
DF: Flagellar motor switch protein FliM
DG: Flagellar motor switch protein FliN
DH: Flagellar motor switch protein FliN
DI: Flagellar motor switch protein FliN
DJ: Flagellar motor switch protein FliG
DK: Flagellar M-ring protein
DL: Flagellar motor switch protein FliM
DM: Flagellar motor switch protein FliM
DN: Flagellar motor switch protein FliN
DO: Flagellar motor switch protein FliN
DP: Flagellar motor switch protein FliN
DQ: Flagellar motor switch protein FliG
DR: Flagellar M-ring protein
DS: Flagellar motor switch protein FliM
DT: Flagellar motor switch protein FliN
DU: Flagellar motor switch protein FliN
DV: Flagellar motor switch protein FliN
DW: Flagellar motor switch protein FliG
DX: Flagellar M-ring protein
DY: Flagellar motor switch protein FliM
DZ: Flagellar motor switch protein FliN
Da: Flagellar motor switch protein FliN
Db: Flagellar motor switch protein FliN
Dc: Flagellar motor switch protein FliG
Dd: Flagellar M-ring protein
De: Flagellar motor switch protein FliM
Df: Flagellar motor switch protein FliN
Dg: Flagellar motor switch protein FliN
Dh: Flagellar motor switch protein FliN
Di: Flagellar motor switch protein FliG
Dj: Flagellar M-ring protein
Dk: Flagellar motor switch protein FliM
Dl: Flagellar motor switch protein FliN
Dm: Flagellar motor switch protein FliN
Dn: Flagellar motor switch protein FliN
Do: Flagellar motor switch protein FliG
Dp: Flagellar M-ring protein
Dq: Flagellar motor switch protein FliM
Dr: Flagellar motor switch protein FliN
Ds: Flagellar motor switch protein FliN
Dt: Flagellar motor switch protein FliN
Du: Flagellar motor switch protein FliG
Dv: Flagellar M-ring protein
Dw: Flagellar motor switch protein FliM
Dx: Flagellar motor switch protein FliN
Dy: Flagellar motor switch protein FliN
Dz: Flagellar motor switch protein FliN
D1: Flagellar motor switch protein FliG
D2: Flagellar M-ring protein
D3: Flagellar motor switch protein FliM
D4: Flagellar motor switch protein FliN
D5: Flagellar motor switch protein FliN
D6: Flagellar motor switch protein FliN
D7: Flagellar motor switch protein FliG
D8: Flagellar M-ring protein
D9: Flagellar motor switch protein FliM
D0: Flagellar motor switch protein FliN
EA: Flagellar motor switch protein FliN
EB: Flagellar motor switch protein FliN
B0: Flagellar motor switch protein FliN
B1: Flagellar motor switch protein FliM
B2: Flagellar motor switch protein FliN
B3: Flagellar motor switch protein FliN
B4: Flagellar motor switch protein FliN
B5: Flagellar motor switch protein FliG
B6: Flagellar M-ring protein
B7: Flagellar motor switch protein FliM
B8: Flagellar motor switch protein FliN
B9: Flagellar motor switch protein FliN
EC: Flagellar motor switch protein FliN
ED: Flagellar motor switch protein FliN
EE: Flagellar motor switch protein FliG
EF: Flagellar M-ring protein
EG: Flagellar motor switch protein FliM
EH: Flagellar motor switch protein FliN
EI: Flagellar motor switch protein FliN
EJ: Flagellar motor switch protein FliN
EK: Flagellar motor switch protein FliG
EL: Flagellar M-ring protein
EM: Flagellar motor switch protein FliM
EN: Flagellar motor switch protein FliN
EO: Flagellar motor switch protein FliN
EP: Flagellar motor switch protein FliN
EQ: Flagellar motor switch protein FliG
ER: Flagellar M-ring protein
ES: Flagellar motor switch protein FliM
ET: Flagellar motor switch protein FliN
EU: Flagellar motor switch protein FliN
EV: Flagellar motor switch protein FliN
EW: Flagellar motor switch protein FliG
EX: Flagellar M-ring protein
EY: Flagellar motor switch protein FliM
EZ: Flagellar motor switch protein FliN
BY: Flagellar motor switch protein FliN
BZ: Flagellar motor switch protein FliN
Ba: Flagellar motor switch protein FliG
Bb: Flagellar M-ring protein
Bc: Flagellar motor switch protein FliM
Bd: Flagellar motor switch protein FliN
Be: Flagellar motor switch protein FliN
Bf: Flagellar motor switch protein FliN
Bg: Flagellar motor switch protein FliG
Bh: Flagellar M-ring protein
Bi: Flagellar motor switch protein FliM
Bj: Flagellar motor switch protein FliN
Bk: Flagellar motor switch protein FliN
Bl: Flagellar motor switch protein FliN
Bm: Flagellar motor switch protein FliG
Bn: Flagellar M-ring protein
Bo: Flagellar motor switch protein FliM
Bp: Flagellar motor switch protein FliN
Bq: Flagellar motor switch protein FliN
Br: Flagellar motor switch protein FliN
Bs: Flagellar motor switch protein FliG
Bt: Flagellar M-ring protein
Bu: Flagellar motor switch protein FliM
Bv: Flagellar motor switch protein FliN
Bw: Flagellar motor switch protein FliN
Bx: Flagellar motor switch protein FliN
By: Flagellar motor switch protein FliG
Bz: Flagellar M-ring protein
C1: Flagellar motor switch protein FliN
C2: Flagellar motor switch protein FliN
CA: Flagellar motor switch protein FliG
CB: Flagellar M-ring protein
CC: Flagellar motor switch protein FliM
CD: Flagellar motor switch protein FliN
CE: Flagellar motor switch protein FliN
CF: Flagellar motor switch protein FliN
CG: Flagellar motor switch protein FliG
CH: Flagellar M-ring protein
CI: Flagellar motor switch protein FliM
CJ: Flagellar motor switch protein FliN
CK: Flagellar motor switch protein FliN
CL: Flagellar motor switch protein FliN
CM: Flagellar motor switch protein FliG
CN: Flagellar M-ring protein
CO: Flagellar motor switch protein FliM
CP: Flagellar motor switch protein FliN
CQ: Flagellar motor switch protein FliN
CR: Flagellar motor switch protein FliN
CS: Flagellar motor switch protein FliG
CT: Flagellar M-ring protein
CU: Flagellar motor switch protein FliM
CV: Flagellar motor switch protein FliN
CW: Flagellar motor switch protein FliN
CX: Flagellar motor switch protein FliN
CY: Flagellar motor switch protein FliG
CZ: Flagellar M-ring protein
Ca: Flagellar motor switch protein FliM
Cb: Flagellar motor switch protein FliN
Cc: Flagellar motor switch protein FliN
Cd: Flagellar motor switch protein FliN
Ce: Flagellar motor switch protein FliG
Cf: Flagellar M-ring protein
Cg: Flagellar motor switch protein FliM
Ch: Flagellar motor switch protein FliN
Ci: Flagellar motor switch protein FliN
Cj: Flagellar motor switch protein FliN
Ck: Flagellar motor switch protein FliG
Cl: Flagellar M-ring protein
Cm: Flagellar motor switch protein FliM
Cn: Flagellar motor switch protein FliN
Co: Flagellar motor switch protein FliN
Cp: Flagellar motor switch protein FliN
Cq: Flagellar motor switch protein FliG
Cr: Flagellar M-ring protein
Cs: Flagellar motor switch protein FliM
Ct: Flagellar motor switch protein FliN
Cu: Flagellar motor switch protein FliN
Cv: Flagellar motor switch protein FliN
Cw: Flagellar motor switch protein FliG
Cx: Flagellar M-ring protein
Cy: Flagellar motor switch protein FliM
Cz: Flagellar motor switch protein FliN
Ea: Flagellar motor switch protein FliG
Eb: Flagellar M-ring protein
Ec: Flagellar motor switch protein FliM
Ed: Flagellar motor switch protein FliN
Ee: Flagellar motor switch protein FliN
Ef: Flagellar motor switch protein FliN
Eg: Flagellar motor switch protein FliG
Eh: Flagellar M-ring protein
Ei: Flagellar motor switch protein FliM
Ej: Flagellar motor switch protein FliN
Ek: Flagellar motor switch protein FliN
El: Flagellar motor switch protein FliN
Em: Flagellar motor switch protein FliG
En: Flagellar M-ring protein
Eo: Flagellar motor switch protein FliM
Ep: Flagellar motor switch protein FliN


Theoretical massNumber of molelcules
Total (without water)14,880,954417
Polymers14,880,954417
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Flagellar basal-body rod protein ... , 3 types, 35 molecules 0123456789AFAGAHAIAJAKALAMANZAZBZCZDZEAAABACADAEBA...

#1: Protein ...
Flagellar basal-body rod protein FlgG / Distal rod protein


Mass: 27784.807 Da / Num. of mol.: 24 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P0A1J3
#5: Protein
Flagellar basal-body rod protein FlgF / Putative proximal rod protein


Mass: 26121.223 Da / Num. of mol.: 5 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P16323
#10: Protein
Flagellar basal-body rod protein FlgC / Putative proximal rod protein


Mass: 13991.889 Da / Num. of mol.: 6 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P0A1I7

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Protein , 6 types, 202 molecules ABCDEFGHIJKLMNOPQRSTUVWXYZA0A6A7A8...

#2: Protein ...
Flagellar L-ring protein / Basal body L-ring protein


Mass: 24726.666 Da / Num. of mol.: 26 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P0A1N8
#3: Protein
Flagellar basal body rod protein FlgB / Putative proximal rod protein


Mass: 15145.061 Da / Num. of mol.: 5 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P16437
#4: Protein
Flagellar hook-basal body complex protein FliE


Mass: 11087.662 Da / Num. of mol.: 6 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P26462
#6: Protein ...
Flagellar M-ring protein


Mass: 61295.645 Da / Num. of mol.: 110 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P15928
#11: Protein ...
Flagellar hook protein FlgE


Mass: 42233.152 Da / Num. of mol.: 29 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P0A1J1
#12: Protein ...
Flagellar P-ring protein / Basal body P-ring protein


Mass: 38194.176 Da / Num. of mol.: 26 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P15930

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Flagellar biosynthetic protein ... , 3 types, 10 molecules AbAqArAsAtAuAvAwAxAy

#7: Protein
Flagellar biosynthetic protein FliQ


Mass: 9606.758 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P0A1L5
#8: Protein Flagellar biosynthetic protein FliR


Mass: 28938.865 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P54702
#9: Protein
Flagellar biosynthetic protein FliP


Mass: 26801.086 Da / Num. of mol.: 5 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P54700

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Flagellar motor switch protein ... , 3 types, 170 molecules C4C5C9C0DADCDGDHDIDNDODPDTDUDVDZDaDbDfDgDhDlDmDnDrDsDtDxDyDz...

#13: Protein ...
Flagellar motor switch protein FliN


Mass: 14801.823 Da / Num. of mol.: 102 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P26419
#14: Protein ...
Flagellar motor switch protein FliG


Mass: 36890.957 Da / Num. of mol.: 34 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P0A1J9
#15: Protein ...
Flagellar motor switch protein FliM


Mass: 37901.066 Da / Num. of mol.: 34 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
References: UniProt: P26418

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: C ring-containing flagellar motor-hook complex in the CCW state
Type: COMPLEX / Entity ID: all / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
Buffer solutionpH: 8
Buffer component
IDConc.NameFormulaBuffer-ID
110 mMtris(hydroxymethyl)aminomethane hydrochlorideTris-HCl1
25 mMethylenediaminetetraacetic acidEDTA1
30.1 % v/voctylphenol ethoxylateTX-1001
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: GOLD / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R0.6/1
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277.15 K
Details: Blot time: 2 Blot force: -5 Wait time: 5 Blot total: 1 Drain time: 2

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN
Electron lensMode: BRIGHT FIELD / Nominal magnification: 81000 X / Nominal defocus max: 1800 nm / Nominal defocus min: 1500 nm / Cs: 2.7 mm
Image recordingElectron dose: 41 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM software
IDNameVersionCategory
1cryoSPARC4.1.1particle selection
2EPUimage acquisition
4cryoSPARC4.1.1CTF correction
7UCSF Chimera1.17model fitting
8UCSF ChimeraX1.5model fitting
10cryoSPARC4.1.1initial Euler assignment
11cryoSPARC4.1.1final Euler assignment
13cryoSPARC4.1.13D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNum. of particles selected: 19627 / Details: Particles were manually picked using cryoSPARC
SymmetryPoint symmetry: C34 (34 fold cyclic)
3D reconstructionResolution: 7.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 10318 / Symmetry type: POINT
Atomic model buildingProtocol: RIGID BODY FIT
Atomic model building
ID 3D fitting-IDSource nameTypeDetailsAccession codeInitial refinement model-ID
11AlphaFoldin silico model
21Otherin silico modelModel-Angelo
31PDBexperimental model7CBL3
41PDBexperimental model7CGO

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