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- PDB-8w90: crystal structure of CD4-D1D2 with Nb457 -

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Basic information

Entry
Database: PDB / ID: 8w90
Titlecrystal structure of CD4-D1D2 with Nb457
Components
  • NB457
  • T-cell surface glycoprotein CD4
KeywordsANTIVIRAL PROTEIN/IMMUNE SYSTEM / inhibitor / complex / antiviral / ANTIVIRAL PROTEIN / ANTIVIRAL PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


helper T cell enhancement of adaptive immune response / interleukin-16 binding / interleukin-16 receptor activity / maintenance of protein location in cell / T cell selection / MHC class II protein binding / positive regulation of kinase activity / cellular response to granulocyte macrophage colony-stimulating factor stimulus / interleukin-15-mediated signaling pathway / positive regulation of monocyte differentiation ...helper T cell enhancement of adaptive immune response / interleukin-16 binding / interleukin-16 receptor activity / maintenance of protein location in cell / T cell selection / MHC class II protein binding / positive regulation of kinase activity / cellular response to granulocyte macrophage colony-stimulating factor stimulus / interleukin-15-mediated signaling pathway / positive regulation of monocyte differentiation / Nef Mediated CD4 Down-regulation / Alpha-defensins / regulation of T cell activation / Other interleukin signaling / extracellular matrix structural constituent / T cell receptor complex / enzyme-linked receptor protein signaling pathway / Translocation of ZAP-70 to Immunological synapse / Phosphorylation of CD3 and TCR zeta chains / macrophage differentiation / regulation of calcium ion transport / Generation of second messenger molecules / T cell differentiation / Co-inhibition by PD-1 / positive regulation of protein kinase activity / Binding and entry of HIV virion / coreceptor activity / positive regulation of interleukin-2 production / positive regulation of calcium-mediated signaling / cell surface receptor protein tyrosine kinase signaling pathway / protein tyrosine kinase binding / Vpu mediated degradation of CD4 / clathrin-coated endocytic vesicle membrane / calcium-mediated signaling / positive regulation of T cell activation / MHC class II protein complex binding / transmembrane signaling receptor activity / Downstream TCR signaling / Cargo recognition for clathrin-mediated endocytosis / signaling receptor activity / Clathrin-mediated endocytosis / positive regulation of protein phosphorylation / virus receptor activity / defense response to Gram-negative bacterium / adaptive immune response / positive regulation of canonical NF-kappaB signal transduction / positive regulation of viral entry into host cell / early endosome / cell surface receptor signaling pathway / positive regulation of ERK1 and ERK2 cascade / positive regulation of MAPK cascade / cell adhesion / immune response / membrane raft / endoplasmic reticulum lumen / external side of plasma membrane / lipid binding / endoplasmic reticulum membrane / protein kinase binding / positive regulation of DNA-templated transcription / enzyme binding / signal transduction / protein homodimerization activity / zinc ion binding / identical protein binding / plasma membrane
Similarity search - Function
CD4, extracellular / T cell CD4 receptor C-terminal region / CD4, extracellular / T cell CD4 receptor C terminal region / T-cell surface antigen CD4 / Immunoglobulin C2-set / Immunoglobulin C2-set domain / Immunoglobulin / Immunoglobulin domain / Immunoglobulin subtype 2 ...CD4, extracellular / T cell CD4 receptor C-terminal region / CD4, extracellular / T cell CD4 receptor C terminal region / T-cell surface antigen CD4 / Immunoglobulin C2-set / Immunoglobulin C2-set domain / Immunoglobulin / Immunoglobulin domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
T-cell surface glycoprotein CD4
Similarity search - Component
Biological speciesVicugna pacos (alpaca)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.81 Å
AuthorsWang, X.Y. / Wang, Y.X.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Structure of CD4-D1D2 domain and nanobody Nb457 at 1.78 Angstroms resolution
Authors: Wang, X.Y. / Wang, Y.X.
History
DepositionSep 4, 2023Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Sep 4, 2024Provider: repository / Type: Initial release
Revision 1.1Nov 6, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: NB457
B: T-cell surface glycoprotein CD4
C: NB457
D: T-cell surface glycoprotein CD4
E: NB457
F: T-cell surface glycoprotein CD4


Theoretical massNumber of molelcules
Total (without water)102,9296
Polymers102,9296
Non-polymers00
Water3,783210
1
A: NB457
B: T-cell surface glycoprotein CD4


Theoretical massNumber of molelcules
Total (without water)34,3102
Polymers34,3102
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: NB457
D: T-cell surface glycoprotein CD4


Theoretical massNumber of molelcules
Total (without water)34,3102
Polymers34,3102
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
E: NB457
F: T-cell surface glycoprotein CD4


Theoretical massNumber of molelcules
Total (without water)34,3102
Polymers34,3102
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)47.082, 141.978, 81.662
Angle α, β, γ (deg.)90.000, 97.931, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

#1: Antibody NB457


Mass: 14584.195 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vicugna pacos (alpaca) / Production host: Escherichia coli BL21(DE3) (bacteria)
#2: Protein T-cell surface glycoprotein CD4


Mass: 19725.414 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CD4 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P01730
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 210 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.63 Å3/Da / Density % sol: 53.17 %
Crystal growTemperature: 289.15 K / Method: vapor diffusion, hanging drop
Details: 0.1 M sodium cacodylate trihydrate pH 6.8, 1.4 M sodium acetate trihydrate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.97853 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 26, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97853 Å / Relative weight: 1
ReflectionResolution: 1.81→50 Å / Num. obs: 96019 / % possible obs: 99.3 % / Redundancy: 6.5 % / Rrim(I) all: 0.07 / Net I/σ(I): 25.7
Reflection shellResolution: 1.81→1.84 Å / Num. unique obs: 4708 / CC1/2: 0.879

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Processing

Software
NameVersionClassification
PHENIXdev_3942refinement
PHASERphasing
HKL-3000data reduction
HKL-3000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1CDH/5LHR

1cdh

5lhr


Resolution: 1.81→24.25 Å / Cross valid method: NONE / Phase error: 21.2888
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2064 4749 -
Rwork0.1863 91185 -
obs-95948 99.26 %
Solvent computationSolvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 1.81→24.25 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6726 0 0 210 6936
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_angle_d0.75199207
X-RAY DIFFRACTIONf_chiral_restr0.05521059
X-RAY DIFFRACTIONf_plane_restr0.00431177
X-RAY DIFFRACTIONf_dihedral_angle_d17.71792498
LS refinement shellResolution: 1.81→1.875 Å
RfactorNum. reflection% reflection
Rfree0.2772 474 -
Rwork0.2479 --
obs-9449 98.59 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.55549404924-0.668915798532-0.4465262126382.114693748640.2283026236491.967967564010.0981731738631-0.404968749173-0.2037841030660.0226116739520.2443236850260.30799170786-0.07451953214920.0972456190925-0.3588847715260.2474868106720.0436752036264-0.007959501272410.230273741349-0.02910616233940.29338197987-4.9298910874256.512075337231.2382810389
23.10124094895-2.582015791663.373590409213.53937176445-1.781851045484.521590031070.1610988873780.2930560298370.33708810726-0.04193830626830.071345365405-0.682911628205-0.03425565876110.588582801949-0.1347032815210.2817885239840.02212696689840.05405878800370.279004884824-0.04059669638010.5351131106130.4110693616656.687867349528.715618683
31.84944014366-1.04000191224-0.307079019542.784165564970.06652146202032.883010639970.022171544069-0.302931206831-0.1773959362470.2730321906580.198913666114-0.335074806021-0.05446949038210.61006041618-0.2043270353070.2203111628220.0522547027611-0.03413534404310.284538761254-0.03974934891480.2814337750411.5753199250547.024221879728.8826215481
44.40831049419-0.383072819366-3.872719254520.741864834725-0.02638804750864.6369016598-0.169556802120.0433646270404-0.7984586301650.181485779156-0.2151453723520.04579859401810.0947050798033-0.1699467953550.4065120139430.2661836675130.02572162703930.03983613626430.2108235868660.009264024616420.315069676829-9.3744851455743.441064634230.0833469682
52.42538992247-1.65405449833-2.042225702922.783778669350.9205563561511.820414931560.1064460255270.2787364666990.199939939886-0.49573078477-0.0262129445208-0.164376062986-0.2375849366970.0582715356785-0.07291364161450.2665213225480.02883885807850.009644466807120.2364538574630.01965865756990.256651786528-0.054785335592447.197280941919.0299715236
66.99369780357-0.1817884225970.8016440170762.147242620830.5305018868432.24740815722-0.0487411023628-0.2918445086891.09283236194-0.2909912807110.324867420262-0.617959296773-0.6338111545140.504945949435-0.1921681906570.33435622435-0.05185864162740.0527144680020.333307596593-0.1000222915950.533810403583.749125198455.714991465124.8622557544
73.554491400920.295333721196-2.672722183883.73544648157-2.469149244757.60451150838-0.007773991816530.331674607484-0.165651444921-0.4586070937140.433239893181.0468256310.304001341957-0.820268161-0.3366348343080.286034728040.0285224686851-0.04344922455110.2997148635790.05737815070210.44982420311-16.552644382752.748945126823.2249817589
81.211150714350.0450052020577-0.9120387284762.228867712791.363970417211.56245450386-0.207051805122-0.22337402770.06385467805880.3960047318160.325447142525-0.2265339025830.1510919857110.39437792201-0.07947048587840.247188143650.0676819982748-0.01219983758360.2551983728050.006487683753060.2472629919031.1620509637442.701061267828.7653562357
92.32258938499-1.46213632475-0.3744036241814.086066468060.8655568309822.895956352-0.251535481432-0.3388806354250.06908461491930.4203207465610.3641276546340.2542761970650.03249584610770.0994232415621-0.08438428561080.2360342605380.03931979367410.01189696024730.2401004521450.02570424982230.228259533508-5.1832252286849.136592957933.2151861866
103.358830226830.4112778442840.01387275671781.603206669080.5183712145261.63925092922-0.02614100541750.178387751580.0393308171404-0.2102640687030.111874962767-0.157166162711-0.08599782353830.226250264838-0.08926405309340.2067020614610.002799571763680.04860538251120.250535207508-0.004443304444450.23581212359312.485706327529.81859511310.9238867627
113.28803279856-0.545075738835-1.477248601380.8084717434210.479868709353.28430054118-0.0506481071082-0.221956303669-0.1970965035190.1243236634220.164092992302-0.09469731656790.1391691427470.457172673395-0.09453338332690.2232539746640.007436550833340.01233316142190.278542553794-0.02702080358160.27694454124814.424002695923.44863864559.82487635613
123.25832058454-0.0919344564504-0.03389359481083.10728931292-0.06483794397653.04134801271-0.04893655531130.04738622732870.0627763811836-0.03217231189650.183893845882-0.159353401257-0.02859118577840.146029277534-0.09213769179910.1786138749330.02509769531780.02440322103620.21787691831-0.01458525915430.2539759680728.5239115227530.213705711314.2609313162
136.344280256683.2894814179-3.51242972114.56243233991-2.585293057594.28821784761-0.165076096640.2016203002460.0995625146742-0.4100613492520.1249771979-0.0312910162609-0.0326450105887-0.1774846074330.09902106559640.2258315296960.0214948128330.009994025208570.2707667848170.03062261339690.2818155911770.56628787795831.224698850213.879416208
144.752702458193.23579752338-2.74972058194.14605938736-1.511064165562.126646753860.0985631311431-0.475424338949-0.3862347864910.202523883952-0.307879564271-0.3289622966930.1756122641240.3839562553610.2711880631280.29807443633-0.02165371306520.07570231615120.3715661558740.04065055384990.409545140618-17.700910368718.203936728530.0457074136
151.912617618890.1284746399710.8180668122561.190296679580.4163998444921.94697007629-0.07902051504280.247843365669-0.103840305138-0.03704515911330.01947721609190.1635071289180.117397662647-0.1350518602830.08315915512360.1949430826150.0219759987020.04279928755080.264730990321-0.04690188599020.333548962833-17.431172305924.926551176317.2464120068
161.45092020933-0.0557279426051-0.5663293067391.51920505986-0.3158358957552.66908025006-0.100899826452-0.065681520189-0.1397623962360.0316430702803-0.06368977992560.1343680178240.3345326232190.02144371032290.153765212710.233739080776-0.004405520376660.04976720126540.268186199205-0.02359954396850.347031038759-17.045610516919.625010755819.9302529148
170.1119733841650.2473518674830.2911500114892.03690611999-0.2046106755131.59621997496-0.1637934014130.2876442831090.0999279965152-0.3547339250530.2189419150110.242237825134-0.0275477667365-0.113029377876-0.06415982851940.328631828816-0.0486889214610.00683537410590.2769703736070.02109056367640.25953898981615.92960729032.94919759406-36.5080420416
182.24005800394-0.0434584855467-1.684263202532.07497704782-0.05498921628273.84881736968-0.00713568375802-0.0557430603934-0.0439500113866-0.0560344490525-0.0380417574048-0.0637744963754-0.09964355037680.08942325292960.05812209745270.245851342255-0.02036728901090.00637745296760.1776713950690.009020663135440.22642947215219.74427510125.91399865378-28.2583102275
192.090256415630.301865983965-0.6795699858562.167779690570.7865759039272.47449856205-0.05994075284280.01922312118420.105715045237-0.0033051858033-0.1472719404620.262026086481-0.275949837541-0.324510639790.177878461830.2364027393470.03441914383440.01706178808910.262612589585-0.03816475283290.2514948699425.9365239282115.7941099638-7.58733582444
203.72050841864-0.6405655539230.8468429070131.46483130222-0.2881030824511.572812389180.01433851426970.0396865951128-0.281377370058-0.0877645440282-0.1929446852590.433472273826-0.17939886398-0.4452674389070.1959887161290.2943127504740.01575555620910.07586284820420.395462382575-0.06255717746240.378481692356-0.6750226635299.38900275173-4.25751341728
212.42048885604-0.664552917335-0.9169199828962.197859788351.429297270232.707803124180.04900603761450.0185037227885-0.145952725042-0.181039979328-0.1937968039050.270410341904-0.167699893145-0.3198165307650.1588044648260.2126990472970.0195197317612-0.001561998453870.27622616304-0.006564867253290.2235961319854.6949548912215.7245498241-5.27634102431
224.30510982541-1.382032443680.7241555167262.27887718061-0.8920437580643.48277052063-0.161825601132-0.101177257652-0.593682505940.292503291264-0.0787722756104-0.1025559707090.1466583396890.4904350384630.04987249878860.2760920970270.01057868411750.04017459779840.2770481000310.08507489003910.36188413009725.13925827663.49019804397-4.76978888127
232.349381832491.638795507410.7702466705341.681107605050.5288948087260.38112046217-0.00151585532231-0.40923058009-0.3093853777240.2175976590640.187249905877-0.234138575006-0.05809909105380.0530862258844-0.1344471861550.291028733635-0.0113954117785-0.02050003237540.497371361449-0.05263434605770.32721938632832.94844607378.60626856697-4.46677714671
242.58543545169-0.09459071209360.5132208717542.661437493770.1866133174982.239724264380.0385464354637-0.401396919702-0.2990217063170.351495283229-0.141307239165-0.3042088560840.1565069800250.4945655861810.1283879988760.327570553227-0.004747821565990.004314016309050.5662730326790.1016977328120.41640295074232.97925858314.717456527650.514666476226
253.889320480940.274396788705-0.4502528462523.264875747630.2348030166962.800031417740.1326574072870.509894281757-0.1950635129220.182359286501-0.0997518005573-0.278165446185-0.0235125672541-0.188301582721-0.08531426753180.258991589263-0.02543533106730.02905993099750.213961934140.02034034737250.270227574514-9.5420668853924.4824472795-43.721757337
262.481915918853.08151966128-0.4465552052513.89385124912-0.2182833877472.315236829420.424742149653-0.490485711736-0.4847127592990.516057048224-0.510296775713-0.6356545129080.04103105729970.2951309498380.06798523590330.303705579944-0.0244429606652-0.04232571491180.3001463569160.123361843140.421253012661-5.2464977660323.6194043861-39.8241649526
270.813077677268-0.3037231156610.04431565868636.344152992431.437747274443.323740848510.3176549298710.202165683697-0.236902636586-0.45241915706-0.320407009872-1.24468353780.4601120028940.587414589919-0.16070867880.519270012757-0.0321037168906-0.1277097259230.4416947837360.07625431740870.5871950219997.8493222394832.2752329493-36.4516383588
283.213507267850.362275386914-0.01977235079694.888149272890.3976913990043.57342053099-0.17936388140.5490414038920.736256996377-0.511563880662-0.08499242211320.318881710139-0.795902858702-0.2783035975170.226998886980.390444218661-0.00290879241649-0.09503165335160.2924349000030.05612356408020.304497193014-12.284763821536.0501957239-45.2414187621
292.162795782120.7559064595220.9281912146383.348270667180.3034709210322.813269116340.248968522246-0.344402909592-0.1389711506790.837623279862-0.2884708704360.1122320936510.182413209927-0.1708747355640.08197410328990.473960401198-0.1045336524960.01533819803430.2631575065410.01669540376140.28881986017-8.8673945670532.5643125487-31.2746893882
302.43287555691.611174301940.5944219165553.136943600060.004618141152684.790061196030.224685996899-0.231781213627-0.06300195823840.551443641919-0.1562459128510.1641563632480.323169051556-0.297215566801-0.05871638093310.36059296194-0.04779584437660.04066768939020.1986012314030.03148375824220.219451221339-11.966688483425.664625385-37.1519567832
311.372455554510.604287497558-0.5795119600531.66655868343-0.2328450752051.050797684530.22498152802-0.132279893894-0.01409648284660.493471510943-0.196337235223-0.2713944030550.000803916195312-0.0695611799069-0.02878175780880.28975473127-0.0311195131121-0.01199958008590.2220938461190.04359926711960.31224940871-3.4574408316237.1288208271-40.2209527921
324.0459625935-0.314266132197-1.126295616612.66913772004-0.2190028006392.80255394619-0.1182086102580.402682533578-0.298231300861-0.04102303186360.1340648661160.5601820007570.06880801797-0.87020137215-0.0006328031667250.193429061689-0.01623541385490.02286751455130.4266251149090.006844682004890.303606920578-19.54558939524.6440746674-46.6506891868
332.88496895987-0.368893220790.5899262769331.282430514791.591247020442.351711349540.203835429651-0.8109432265070.4577436500780.6098585012250.0271913775937-0.432254548181-0.3284629525210.339938304019-0.224510904360.99023761812-0.223575843961-0.102814959230.593172326768-0.07645552657990.541735104678-0.74487116581350.3474119928-17.833996385
342.38795137637-1.95758492605-0.5410336050725.5188716736-0.2264816729531.08868904840.3077999901110.03828975432210.01885757645390.243072380932-0.3769086528460.385623860492-0.164864425165-0.1781231240560.1497450971180.674457759651-0.1076125950880.07958106582470.382653445797-0.08325867978290.449703127891-16.682922240352.864998989-28.141522359
356.69372597039-1.610043721310.6338542926752.19187236111.089129517624.27109538801-0.1282179802810.0556896385448-0.5001359126130.4780102770620.03260366746931.24832689381.04752744421-0.2440499337370.3973420187980.599792225177-0.084041703635-0.0594663589480.46871707561-0.30682110921.07652274395-30.53059826258.8833219878-34.4514795234
362.55783145114-2.258725514870.1903626121683.69925457659-0.2528873540650.974943813954-0.0743938770634-0.307150015437-0.4392896419580.4771725779070.0813572204240.417176182673-0.03727024793660.06746538842280.02315235976130.690085547556-0.0390245065920.1807864580690.422170915064-0.09215224183550.602602637654-23.242320278558.5497549437-26.9749812187
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 17 )AA1 - 171 - 17
22chain 'A' and (resid 18 through 25 )AA18 - 2518 - 25
33chain 'A' and (resid 29 through 39 )AA29 - 3926 - 36
44chain 'A' and (resid 40 through 51 )AA40 - 5137 - 48
55chain 'A' and (resid 52 through 73 )AA52 - 7349 - 70
66chain 'A' and (resid 74 through 83 )AA74 - 8371 - 80
77chain 'A' and (resid 84 through 91 )AA84 - 9181 - 88
88chain 'A' and (resid 92 through 108 )AA92 - 10889 - 105
99chain 'A' and (resid 109 through 124 )AA109 - 124106 - 121
1010chain 'B' and (resid 1 through 48 )BB1 - 481 - 48
1111chain 'B' and (resid 49 through 68 )BB49 - 6849 - 68
1212chain 'B' and (resid 69 through 88 )BB69 - 8869 - 88
1313chain 'B' and (resid 89 through 102 )BB89 - 10289 - 102
1414chain 'B' and (resid 103 through 119 )BB103 - 119103 - 119
1515chain 'B' and (resid 120 through 140 )BB120 - 140120 - 140
1616chain 'B' and (resid 141 through 178 )BB141 - 178141 - 178
1717chain 'C' and (resid -1 through 33 )CC-1 - 331 - 34
1818chain 'C' and (resid 34 through 124 )CC34 - 12435 - 125
1919chain 'D' and (resid 1 through 38 )DD1 - 381 - 35
2020chain 'D' and (resid 39 through 58 )DD39 - 5836 - 50
2121chain 'D' and (resid 59 through 88 )DD59 - 8851 - 80
2222chain 'D' and (resid 89 through 119 )DD89 - 11981 - 110
2323chain 'D' and (resid 120 through 140 )DD120 - 140111 - 131
2424chain 'D' and (resid 141 through 178 )DD141 - 178132 - 169
2525chain 'E' and (resid 1 through 17 )EE1 - 171 - 17
2626chain 'E' and (resid 18 through 25 )EE18 - 2518 - 25
2727chain 'E' and (resid 26 through 33 )EE26 - 3326 - 33
2828chain 'E' and (resid 34 through 45 )EE34 - 4534 - 45
2929chain 'E' and (resid 46 through 73 )EE46 - 7346 - 73
3030chain 'E' and (resid 74 through 91 )EE74 - 9174 - 91
3131chain 'E' and (resid 92 through 117 )EE92 - 11792 - 117
3232chain 'E' and (resid 118 through 124 )EE118 - 124118 - 124
3333chain 'F' and (resid 1 through 79 )FF1 - 791 - 65
3434chain 'F' and (resid 80 through 136 )FF80 - 13666 - 122
3535chain 'F' and (resid 137 through 154 )FF137 - 154123 - 138
3636chain 'F' and (resid 155 through 177 )FF155 - 177139 - 161

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