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Yorodumi- PDB-8w8p: Thermus thermophilus initiation transcription complex containing ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8w8p | ||||||
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Title | Thermus thermophilus initiation transcription complex containing CMPcPP in the post-translocated state | ||||||
Components |
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Keywords | TRANSCRIPTION/DNA/RNA / Thermus thermophilus / RNA polymerase / transcription initiation / post-translocated / CMPcPP / TRANSCRIPTION / TRANSCRIPTION-DNA-RNA complex | ||||||
Function / homology | Function and homology information sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding ...sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-templated transcription / magnesium ion binding / DNA binding / zinc ion binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Thermus thermophilus HB8 (bacteria) synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.165 Å | ||||||
Authors | Li, L. / Zhang, Y. | ||||||
Funding support | China, 1items
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Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2024 Title: Nanopore tweezers show fractional-nucleotide translocation in sequence-dependent pausing by RNA polymerase. Authors: Nova, I.C. / Craig, J.M. / Mazumder, A. / Laszlo, A.H. / Derrington, I.M. / Noakes, M.T. / Brinkerhoff, H. / Yang, S. / Vahedian-Movahed, H. / Li, L. / Zhang, Y. / Bowman, J.L. / Huang, J.R. ...Authors: Nova, I.C. / Craig, J.M. / Mazumder, A. / Laszlo, A.H. / Derrington, I.M. / Noakes, M.T. / Brinkerhoff, H. / Yang, S. / Vahedian-Movahed, H. / Li, L. / Zhang, Y. / Bowman, J.L. / Huang, J.R. / Mount, J.W. / Ebright, R.H. / Gundlach, J.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8w8p.cif.gz | 738.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8w8p.ent.gz | 583.6 KB | Display | PDB format |
PDBx/mmJSON format | 8w8p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8w8p_validation.pdf.gz | 911.3 KB | Display | wwPDB validaton report |
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Full document | 8w8p_full_validation.pdf.gz | 992.6 KB | Display | |
Data in XML | 8w8p_validation.xml.gz | 124.2 KB | Display | |
Data in CIF | 8w8p_validation.cif.gz | 171.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w8/8w8p ftp://data.pdbj.org/pub/pdb/validation_reports/w8/8w8p | HTTPS FTP |
-Related structure data
Related structure data | 8w8nC 8w8oC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-DNA-directed RNA polymerase subunit ... , 4 types, 5 molecules ABCDE
#1: Protein | Mass: 35056.164 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus HB8 (bacteria) / References: UniProt: Q5SHR6, DNA-directed RNA polymerase #2: Protein | | Mass: 125436.539 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus HB8 (bacteria) / References: UniProt: Q8RQE9, DNA-directed RNA polymerase #3: Protein | | Mass: 170997.391 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus HB8 (bacteria) / References: UniProt: Q8RQE8, DNA-directed RNA polymerase #4: Protein | | Mass: 11533.316 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus HB8 (bacteria) / References: UniProt: Q8RQE7, DNA-directed RNA polymerase |
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-DNA chain , 2 types, 2 molecules GH
#6: DNA chain | Mass: 6440.147 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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#7: DNA chain | Mass: 8421.432 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
-Protein / RNA chain , 2 types, 2 molecules FI
#5: Protein | Mass: 50769.398 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus HB8 (bacteria) / Gene: sigA, TTHA0532 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q5SKW1 |
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#8: RNA chain | Mass: 1134.619 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
-Non-polymers , 4 types, 71 molecules
#9: Chemical | ChemComp-MG / #10: Chemical | #11: Chemical | ChemComp-2TM / | #12: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.14 Å3/Da / Density % sol: 60.8 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop Details: 0.05M Magnesium chloride, 0.2M Potassium chloride, 0.1M Tris-HCl pH8.0, 10% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.9181 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Aug 12, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9181 Å / Relative weight: 1 |
Reflection | Resolution: 3.165→40 Å / Num. obs: 559327 / % possible obs: 96.2 % / Redundancy: 6.3 % / Rmerge(I) obs: 0.244 / Net I/σ(I): 6.74 |
Reflection shell | Resolution: 3.2→3.26 Å / Rmerge(I) obs: 0.938 / Mean I/σ(I) obs: 1.729 / Num. unique obs: 559327 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.165→39.755 Å / SU ML: 0.42 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 29.76 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.165→39.755 Å
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Refine LS restraints |
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LS refinement shell |
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