分子量: 16330.101 Da / 分子数: 4 / 変異: L182D,S183D,E184D,Q185D,R186K / 由来タイプ: 組換発現 詳細: Remainder of protein appears to have undergone autocleavage and has been shed. Molecular weight of the target protein structure matches the molecular weight found on SDS-PAGE. 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TMPRSS11D, HAT / プラスミド: pFHMSP-LIC C 詳細 (発現宿主): baculovirus donor vector for secreted protein production 発現宿主: Spodoptera frugiperda (ツマジロクサヨトウ) 参照: UniProt: O60235
#2: タンパク質
Transmembraneproteaseserine11Dcatalyticchain
分子量: 26944.199 Da / 分子数: 4 / 由来タイプ: 組換発現 詳細: Linked to the non-catalytic domain through a disulfide bond (Cys173-Cys292) after proteolytic cleavage at Lys186/Ile187 peptide bond. 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TMPRSS11D, HAT / プラスミド: pFHMSP-LIC C 詳細 (発現宿主): baculovirus donor vector for secreted protein production 発現宿主: Spodoptera frugiperda (ツマジロクサヨトウ) 参照: UniProt: O60235
温度: 291 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: 0.5 uL protein (30 mg/mL; in 50 mM Tris pH 8.0, 200 mM NaCl) mixed 1:1 with precipitant (20%PEG1500, 0.2M MgCl2, 0.1M HEPES pH 7.5) through automated addition with Art Robbin Phoenix robot. PH範囲: 7.5
構造決定の手法: 分子置換 / 解像度: 1.59→49.77 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.953 / SU B: 1.656 / SU ML: 0.056 / 交差検証法: THROUGHOUT / ESU R: 0.079 / ESU R Free: 0.08 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.1874
1331
1 %
RANDOM
Rwork
0.15502
-
-
-
obs
0.15535
129049
99.99 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK