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- PDB-8v34: Crystal structure of S. aureus TarK N-terminal domain -

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データを開く


IDまたはキーワード:

読み込み中...

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基本情報

登録情報
データベース: PDB / ID: 8v34
タイトルCrystal structure of S. aureus TarK N-terminal domain
要素CDP-glycerol glycerophosphotransferase family protein
キーワードTRANSFERASE / glycosyltransferase / immunoglobulin-like domain
機能・相同性: / CDP-glycerol glycerophosphotransferase activity / CDP-glycerol glycerophosphotransferase / CDP-glycerol glycerophosphotransferase, C-terminal domain / CDP-glycerol glycerophosphotransferase, N-terminal domain / CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase / teichoic acid biosynthetic process / plasma membrane / CDP-glycerol glycerophosphotransferase family protein
機能・相同性情報
生物種Staphylococcus aureus (黄色ブドウ球菌)
手法X線回折 / シンクロトロン / 分子置換 / 解像度: 3 Å
データ登録者Li, F.K.K. / Strynadka, N.C.J.
資金援助 カナダ, 米国, 4件
組織認可番号
Natural Sciences and Engineering Research Council (NSERC, Canada) カナダ
Canadian Institutes of Health Research (CIHR) カナダ
Howard Hughes Medical Institute (HHMI) 米国
Canada Research Chairs カナダ
引用ジャーナル: Sci Adv / : 2024
タイトル: Cryo-EM analysis of TarL, a polymerase in wall teichoic acid biogenesis central to virulence and antibiotic resistance.
著者: Franco K K Li / Liam J Worrall / Robert T Gale / Eric D Brown / Natalie C J Strynadka /
要旨: Wall teichoic acid (WTA), a covalent adduct of Gram-positive bacterial cell wall peptidoglycan, contributes directly to virulence and antibiotic resistance in pathogenic species. Polymerization of ...Wall teichoic acid (WTA), a covalent adduct of Gram-positive bacterial cell wall peptidoglycan, contributes directly to virulence and antibiotic resistance in pathogenic species. Polymerization of the WTA ribitol-phosphate chain is catalyzed by TarL, a member of the largely uncharacterized TagF-like family of membrane-associated enzymes. We report the cryo-electron microscopy structure of TarL, showing a tetramer that forms an extensive membrane-binding platform of monotopic helices. TarL is composed of an amino-terminal immunoglobulin-like domain and a carboxyl-terminal glycosyltransferase-B domain for ribitol-phosphate polymerization. The active site of the latter is complexed to donor substrate cytidine diphosphate-ribitol, providing mechanistic insights into the catalyzed phosphotransfer reaction. Furthermore, the active site is surrounded by electropositive residues that serve to retain the lipid-linked acceptor for polymerization. Our data advance general insight into the architecture and membrane association of the still poorly characterized monotopic membrane protein class and present molecular details of ribitol-phosphate polymerization that may aid in the design of new antimicrobials.
履歴
登録2023年11月26日登録サイト: RCSB / 処理サイト: RCSB
改定 1.02024年4月3日Provider: repository / タイプ: Initial release

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構造の表示

構造ビューア分子:
MolmilJmol/JSmol

ダウンロードとリンク

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集合体

登録構造単位
B: CDP-glycerol glycerophosphotransferase family protein
A: CDP-glycerol glycerophosphotransferase family protein
C: CDP-glycerol glycerophosphotransferase family protein
D: CDP-glycerol glycerophosphotransferase family protein
E: CDP-glycerol glycerophosphotransferase family protein
F: CDP-glycerol glycerophosphotransferase family protein
G: CDP-glycerol glycerophosphotransferase family protein
H: CDP-glycerol glycerophosphotransferase family protein
I: CDP-glycerol glycerophosphotransferase family protein
J: CDP-glycerol glycerophosphotransferase family protein


分子量 (理論値)分子数
合計 (水以外)204,59910
ポリマ-204,59910
非ポリマー00
00
1
B: CDP-glycerol glycerophosphotransferase family protein


分子量 (理論値)分子数
合計 (水以外)20,4601
ポリマ-20,4601
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
2
A: CDP-glycerol glycerophosphotransferase family protein


分子量 (理論値)分子数
合計 (水以外)20,4601
ポリマ-20,4601
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
3
C: CDP-glycerol glycerophosphotransferase family protein


分子量 (理論値)分子数
合計 (水以外)20,4601
ポリマ-20,4601
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
4
D: CDP-glycerol glycerophosphotransferase family protein


分子量 (理論値)分子数
合計 (水以外)20,4601
ポリマ-20,4601
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
5
E: CDP-glycerol glycerophosphotransferase family protein


分子量 (理論値)分子数
合計 (水以外)20,4601
ポリマ-20,4601
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
6
F: CDP-glycerol glycerophosphotransferase family protein


分子量 (理論値)分子数
合計 (水以外)20,4601
ポリマ-20,4601
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
7
G: CDP-glycerol glycerophosphotransferase family protein


分子量 (理論値)分子数
合計 (水以外)20,4601
ポリマ-20,4601
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
8
H: CDP-glycerol glycerophosphotransferase family protein


分子量 (理論値)分子数
合計 (水以外)20,4601
ポリマ-20,4601
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
9
I: CDP-glycerol glycerophosphotransferase family protein


分子量 (理論値)分子数
合計 (水以外)20,4601
ポリマ-20,4601
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
10
J: CDP-glycerol glycerophosphotransferase family protein


分子量 (理論値)分子数
合計 (水以外)20,4601
ポリマ-20,4601
非ポリマー00
0
タイプ名称対称操作
identity operation1_555x,y,z1
単位格子
Length a, b, c (Å)89.885, 93.674, 136.760
Angle α, β, γ (deg.)90.00, 91.48, 90.00
Int Tables number4
Space group name H-MP1211

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要素

#1: タンパク質
CDP-glycerol glycerophosphotransferase family protein


分子量: 20459.881 Da / 分子数: 10 / 断片: residues 1-172 / 由来タイプ: 組換発現
由来: (組換発現) Staphylococcus aureus (黄色ブドウ球菌)
遺伝子: tagF_3 / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: A0A6B5GWD3

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実験情報

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実験

実験手法: X線回折 / 使用した結晶の数: 1

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試料調製

結晶マシュー密度: 2.81 Å3/Da / 溶媒含有率: 56.28 %
結晶化温度: 298 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 8.5
詳細: 0.1 M Tris pH 8.5, 100 mM NaCl, 75 mM MgCl2, 33% PEG 400

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データ収集

回折平均測定温度: 100 K / Serial crystal experiment: N
放射光源由来: シンクロトロン / サイト: ALS / ビームライン: 5.0.2 / 波長: 0.9795 Å
検出器タイプ: DECTRIS PILATUS3 6M / 検出器: PIXEL / 日付: 2018年3月10日
放射プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長波長: 0.9795 Å / 相対比: 1
反射解像度: 3→47.49 Å / Num. obs: 45589 / % possible obs: 99.66 % / 冗長度: 3.4 % / CC1/2: 0.998 / CC star: 0.999 / Net I/σ(I): 11.96
反射 シェル解像度: 3→3.107 Å / 冗長度: 3.6 % / Mean I/σ(I) obs: 1.68 / Num. unique obs: 4546 / CC1/2: 0.676 / CC star: 0.898 / % possible all: 99.74

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解析

ソフトウェア
名称バージョン分類
PHENIX1.19.2_4158精密化
XDSデータ削減
Aimlessデータスケーリング
PHASER位相決定
精密化構造決定の手法: 分子置換 / 解像度: 3→47.49 Å / SU ML: 0.44 / 交差検証法: FREE R-VALUE / σ(F): 1.34 / 位相誤差: 31.05 / 立体化学のターゲット値: ML
Rfactor反射数%反射
Rfree0.2877 2279 5 %
Rwork0.236 --
obs0.2386 45567 99.69 %
溶媒の処理減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL
精密化ステップサイクル: LAST / 解像度: 3→47.49 Å
タンパク質核酸リガンド溶媒全体
原子数13127 0 0 0 13127
拘束条件
Refine-IDタイプDev ideal
X-RAY DIFFRACTIONf_bond_d0.00213396
X-RAY DIFFRACTIONf_angle_d0.56718194
X-RAY DIFFRACTIONf_dihedral_angle_d4.5651743
X-RAY DIFFRACTIONf_chiral_restr0.0472022
X-RAY DIFFRACTIONf_plane_restr0.0042369
LS精密化 シェル
解像度 (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3-3.070.37091420.32722698X-RAY DIFFRACTION100
3.07-3.140.36671410.31092674X-RAY DIFFRACTION100
3.14-3.210.35911420.29462696X-RAY DIFFRACTION100
3.21-3.30.32941410.2992673X-RAY DIFFRACTION100
3.3-3.40.36551420.29152706X-RAY DIFFRACTION100
3.4-3.510.34511430.26682706X-RAY DIFFRACTION100
3.51-3.630.34271400.25432674X-RAY DIFFRACTION100
3.63-3.780.31581420.26552699X-RAY DIFFRACTION100
3.78-3.950.28661420.24742700X-RAY DIFFRACTION100
3.95-4.160.28511440.24392717X-RAY DIFFRACTION100
4.16-4.420.2711410.20762693X-RAY DIFFRACTION100
4.42-4.760.22471430.18622717X-RAY DIFFRACTION100
4.76-5.240.24351420.19012698X-RAY DIFFRACTION100
5.24-60.27451430.21632722X-RAY DIFFRACTION100
6-7.550.33511440.26452735X-RAY DIFFRACTION100
7.55-47.490.24431470.21732780X-RAY DIFFRACTION99
精密化 TLS

手法: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
15.7701-0.18480.89984.38324.935.6520.35560.30770.4414-0.3575-0.2110.1575-0.0169-0.0595-0.14690.72910.03680.13470.42990.01450.536222.472120.040712.988
27.8487-4.3048-0.72444.3426-1.89546.85740.36760.2382-0.278-0.20340.19430.4239-0.0056-0.2979-0.42850.7104-0.08210.10020.529-0.01120.53719.850313.326915.9278
36.9208-0.25560.76049.84710.41157.87430.18920.20920.3738-1.591-0.1722-0.84520.083-0.3058-0.07290.6930.09250.16160.6125-0.10.441425.567614.84437.2986
40.35031.4425-1.19715.0944-4.13653.39730.34820.4932-1.7364-2.2729-1.9678-1.63912.6380.88641.9421.2520.38120.07141.6861-0.06641.613649.924717.62464.2525
59.38034.4443-1.42027.3905-3.36482.7337-1.2329-0.0511-1.8958-0.10220.418-0.9716-0.2872-0.6950.12471.12590.38190.31110.75040.00791.074740.519615.90425.6913
68.8055-4.01751.37913.5724-2.68273.45850.2416-0.92-0.8389-0.23990.28271.76062.0938-0.1833-0.50350.7305-0.1091-0.06180.6822-0.02950.715332.2321-0.012323.3885
75.6821-1.59342.99468.5297-1.57067.30250.31350.7042-0.5212-1.05970.3963-0.5565-0.00550.4593-0.63620.55630.10960.12620.6408-0.16640.563532.3655.92479.8897
84.63784.6581.40384.7444-0.8564.0819-0.24750.67510.4838-0.49070.32920.4492-0.41860.1205-0.28420.5404-0.03980.17510.50010.09460.81214.244825.340531.0418
96.11830.808-2.51999.0819-3.66969.20860.2503-0.04670.3112-0.3995-0.11450.0047-0.00650.3184-0.02630.28220.0270.08680.4009-0.01190.365310.778417.277431.455
100.83930.27350.34343.1572-2.3052.36540.2569-0.62980.17151.7846-0.1023-0.33050.1301-0.0362-0.22350.8987-0.1790.1690.675-0.11190.50979.047312.43441.8718
112.44362.7988-1.26423.4601-2.2767.0074-0.1148-0.41170.58251.62210.24131.6387-0.90851.35940.15640.78470.09040.2120.5862-0.13830.923210.858327.260437.569
121.8398-0.02330.13781.15760.52080.2651-0.1441-0.77822.52190.22180.04721.3393-0.29210.235-0.4532-0.72550.29160.34540.87410.46492.3212-10.21325.417831.7837
131.5177-2.4981-1.13076.73114.5613.77630.4153-0.7535-1.3137-0.37771.2544.5059-1.4053-1.6893-1.34991.7960.3610.15571.10940.99882-20.623320.473839.5403
146.8617-4.9974-0.66583.796-0.99417.70050.8235-0.6210.79651.3118-0.26432.5040.37530.53972.38120.7048-0.09890.77760.7918-0.04781.2644-10.241719.729540.1566
158.02617.2132.43148.6846-1.75358.71841.109-0.3403-1.6146-0.91830.1891-0.96491.49450.6105-1.03470.88950.02650.00780.4684-0.20180.9891.9454-1.913534.6903
165.18690.1321-0.13986.5365-2.52014.43570.7292-1.01760.38451.0941-0.33750.0345-0.0048-0.7657-0.40070.7434-0.13360.06930.6843-0.0490.43940.06911.052541.8758
176.4264-0.48362.00317.21880.02911.9775-0.1822-0.2411-0.3610.48350.01370.08510.6837-0.48150.15780.8559-0.08820.2520.4659-0.02060.568524.598445.594321.313
182.5399-1.17281.19342.48223.33497.4861-2.6157-0.30990.02340.67843.0787-1.01-1.02211.07580.15670.86060.1059-0.04510.5241-0.13970.6522.087652.603520.8571
194.47992.0830.74723.45664.30437.40760.1166-0.9-0.17791.033-0.2933-1.6010.13760.533-0.03580.79660.1249-0.08930.602-0.05290.706932.404156.07826.9811
205.26823.5425-0.23063.4388-1.5657.3645-1.23180.758-1.1466-0.00950.6853-2.3596-0.10420.41770.34940.9231-0.09280.49980.7044-0.09331.579839.538347.04889.1624
217.1430.12031.39525.0826-2.01851.0743-1.0508-2.4410.79432.10020.51712.00860.20191.74880.33831.4053-0.05260.28160.80540.00820.701828.252469.897417.9539
220.42470.53540.33442.5674-1.87633.8343-0.2628-0.0374-0.06651.78830.6555-1.2438-0.43180.483-0.25840.4839-0.08050.05080.6404-0.03580.798736.96257.965319.0371
237.6352-1.55573.01692.3172-3.03187.5384-0.3633-0.53061.03120.02130.020.1411-0.2-0.34040.36220.589-0.2096-0.08141.0463-0.18420.607225.79747.408562.1497
247.91368.41788.27929.15028.33229.0430.8008-1.3223-1.49321.1652-0.0851-1.57532.10221.0748-1.00060.7440.0316-0.12271.3152-0.30690.649933.6577-1.252763.1541
254.79362.2638-0.15142.95133.2716.4951-0.49380.09130.04080.5950.7675-0.15270.65410.7697-0.24990.5939-0.0237-0.05760.7765-0.07060.493227.3866-0.470456.7564
267.34173.70724.04674.40694.85215.07420.2719-0.8644-0.86480.37170.0931-0.93121.5839-0.0805-0.33910.9691-0.2363-0.04311.0043-0.05020.65222.1971-7.57660.8281
273.966-0.30830.71413.62772.03630.9692-0.6634-2.01461.16091.55580.1394-1.03840.69270.3568-0.2010.742-0.2402-0.13621.3557-0.62260.629521.95757.575270.6562
286.11154.01494.92523.41781.58087.3964-1.8602-0.94530.6904-0.0353-0.24280.94040.9058-1.03952.33931.63590.29670.18942.2735-0.61821.12792.116110.390975.2319
294.89712.3291-2.54332.64581.50216.52641.953-1.9561-0.06180.9925-0.61111.2891.266-4.20340.63480.82-0.49460.00242.2735-0.32990.897910.20246.439871.1181
303.25782.35111.25318.3167-3.96724.5104-0.27490.1033-0.36360.00320.31610.5041-0.41242.02140.0040.40230.08890.0340.8719-0.01330.30610.6544-2.354247.0122
313.71660.04152.07096.50712.74869.0170.4088-1.0198-0.10662.1830.26450.57250.45080.2838-0.75790.8631-0.172-0.02220.79660.02650.513413.0777-3.290462.0049
320.8454-0.22680.87788.7012-5.87014.32490.2273-1.2351-0.4039-0.51220.37110.22082.8257-1.1018-0.2460.70210.1255-0.12451.21340.07570.63946.898659.94751.3233
332.129-1.89290.34489.0071-2.25472.8817-0.1701-0.45340.00370.38750.24490.3325-0.1233-0.5298-0.00860.65240.0439-0.03070.91450.12350.680640.152665.863951.5957
344.3632-3.34763.00947.9711-0.30883.6197-0.3771-0.47460.89690.9496-0.085-1.2054-1.8565-0.03130.61020.8044-0.1839-0.12110.63720.02930.708843.501175.696945.3939
359.22191.6927-6.1933.2973-1.10764.15351.00870.14112.78431.39020.0933-2.48960.31182.2767-0.87160.6690.0682-0.37021.2690.20641.065648.64668.368158.8168
362.2706-0.4293-2.38595.1774.19595.2588-0.8758-1.6798-1.71522.08280.60780.18351.43133.86240.09520.83560.40160.21771.45540.09040.910258.186753.840742.6866
374.6387-6.35422.92962.0265-1.56212.02072.18323.82150.8059-1.9707-3.3746-2.7377.05712.2750.10793.05360.52630.02361.61510.41551.443565.641457.797233.1208
383.761.54863.46888.07250.82843.6655-0.7298-0.5418-1.42990.3209-0.1338-1.3359-1.36661.29340.81680.7258-0.1318-0.07261.48880.46750.890558.918662.844639.3965
395.22242.4128-1.14023.6673.15955.5968-0.291-0.17120.2595-1.5043-0.2740.8686-2.1676-1.44890.10630.73150.1653-0.10160.7073-0.17420.494937.344469.784430.8281
403.9155-2.6261.05936.9304-2.51818.73-1.221-0.25790.12080.0929-0.6554-1.8504-1.67071.58361.66280.7428-0.0932-0.1340.91480.15080.680149.004171.560139.1077
418.2322-2.64691.79153.28072.13413.6458-0.7216-0.45780.18022.026-1.3299-3.2959-0.93431.6621.570.9085-0.0655-0.58210.93850.39341.6292-12.919235.60223.5097
424.43221.35684.33483.6045-1.77727.22270.3888-1.5676-0.7852-0.752-0.4178-0.02441.88940.64970.5471.2760.2864-0.23960.90860.25021.5255-0.867924.60668.8461
437.43311.0617-4.38920.3671-1.21635.674-0.66290.4184-2.1842-0.37080.3172-3.29711.69810.06670.29930.49680.2955-0.00530.54620.15111.9614-14.377620.5205-3.3887
444.14933.1847-0.98088.0408-2.84675.2708-0.2815-0.7988-0.74850.1793-0.1001-2.4193-1.38181.347-0.08140.60350.09040.09720.95970.11281.4302-7.480431.8971-1.3484
459.4832.61065.08388.63361.62539.94080.5015-1.6812-1.05230.3366-0.0211-2.14230.9833-0.3624-0.33750.75110.0565-0.04970.93780.30111.1629-16.650725.80082.3257
464.35194.68792.63725.76391.2159.1407-0.6919-2.9092-0.0373-0.3292-0.93761.5493-0.8206-2.29142.04511.82980.4359-0.09441.4533-0.20281.0542-34.135237.643216.9936
478.8372-0.0669-2.75863.2151-4.026.6210.3842-1.44221.33520.37531.19450.0928-2.43940.4041-1.25281.04850.10360.07581.3803-0.13210.5456-28.030731.408311.6269
484.745-1.49544.70227.0376-0.79799.26331.1042-0.5486-0.5657-0.9595-0.57080.6291.0604-0.2363-0.30350.7220.13480.02580.58150.08450.9042-25.260331.5495-4.2427
493.2483-3.04022.47247.2023-4.94713.42391.5323-0.8818-0.88590.30490.33041.56810.6052-0.6981-1.76410.78490.15770.0070.63240.0990.7846-26.251628.7876-3.3952
506.6282-2.0817-3.01776.95341.54225.7067-0.43130.5643-0.6446-0.6449-0.00940.20120.4127-0.61850.38770.6972-0.14130.08480.5252-0.04820.54622.373753.355624.7792
516.83133.5422-1.48135.4509-1.26474.5645-0.3101-0.4985-0.43060.1588-0.43760.5781-0.0244-0.35480.6580.74350.12760.08770.5391-0.10660.6212-8.107954.351238.6003
520.82330.20311.50749.28214.57345.7696-1.316-0.23150.0602-1.18812.1072-0.3313-0.92191.0483-0.87440.6954-0.28860.07470.9045-0.11890.811449.286710.159948.3231
538.33210.6137-0.99384.0185-4.75386.5538-0.50280.4601-0.01660.51070.7138-0.36860.1186-0.0206-0.1840.5403-0.26450.08931.1307-0.35930.613245.89465.820648.8752
545.2896-1.0722-2.0844.76770.70866.3141-0.2937-0.64191.1657-0.56670.20390.1541-1.08040.09210.22730.669-0.1652-0.13160.7194-0.14180.765638.887710.396645.659
555.6032-3.1883-1.40133.22420.73358.70430.318-0.56261.1343-0.1170.941-0.96-1.57180.6278-0.55490.7232-0.27920.06930.6757-0.23390.733845.646112.093739.9925
568.3586-0.23165.18545.9973-3.26564.90081.78771.1986-0.4807-2.5586-0.2360.99350.83972.4349-1.54541.1758-0.1507-0.10821.8762-0.64361.73568.8970.257136.0737
574.02965.01960.44538.5097-1.63272.7509-0.32880.128-1.6696-1.0959-1.29440.5456-1.19981.15572.39531.0835-0.35140.22691.16850.04331.312660.52074.896734.6184
585.87710.88540.6772.73982.85243.3396-0.3884-0.33810.6490.0807-0.12761.75690.9005-0.36160.12760.8732-0.1293-0.11310.72340.03760.885138.8203-8.565434.1554
592.31580.5012.27127.59273.71684.0881-0.44080.1740.4312-1.72210.1349-0.3816-1.29261.49720.13341.154-0.35090.04720.8925-0.0740.538646.81043.664532.1338
606.44680.00460.0216.21440.92270.6126-0.2641-0.5342-0.4463-0.53980.79990.60530.44120.3533-0.48440.7688-0.1182-0.14291.11580.34410.718624.226556.977358.5067
613.8845-2.36481.66944.4672-3.21428.81530.3111-0.4293-1.3614-0.36930.2896-0.34241.2374-0.5324-0.43921.1117-0.1573-0.26110.84130.23021.331116.485553.940452.4437
621.60991.02940.19314.9217-3.79523.62770.7296-1.8644-0.56022.24260.59051.1718-0.8168-1.3781-1.4381.18940.0710.06372.32110.90421.56726.325961.161773.6441
633.8795-3.02844.42924.3667-2.53784.72840.7852-1.3193-1.6961-1.2364-0.25320.81280.6819-0.1802-0.38610.4022-0.13540.09580.92650.340.98059.257667.020654.5407
642.18913.68113.63995.14655.72014.99-0.3214-0.76110.3219-0.9413-0.46290.92650.2013-0.43090.7011.21880.0567-0.09270.8811-0.07210.719814.620639.2787-4.6065
656.81255.2761.50326.54410.71099.38170.0098-0.94770.72531.9850.03460.92120.4078-0.23270.03731.12770.15560.09610.695-0.00280.64253.803538.625-9.79
664.7963-0.8565.62314.6142-1.31776.4642-0.89041.02361.39110.0043-0.63580.2444-0.54230.61251.78591.0916-0.0944-0.07920.75680.16380.9064.320844.2736-19.845
678.30876.5504-2.75485.2-2.17250.9264-0.07720.21623.54341.8358-0.25621.25371.51520.4660.89721.3473-0.0773-0.42741.0523-0.13661.1410.781547.8185-6.1257
686.04240.78672.33876.61875.68215.3835-0.41620.4871-0.2593-0.95152.362-1.3929-2.4663.6252-2.13851.0277-0.3102-0.15521.9538-0.14081.518722.880233.9977-16.1759
692.2322-2.27981.51953.7841-2.72512.92260.6045-1.12010.39220.1044-0.29170.4136-0.0517-0.6842-0.62360.7772-0.11650.0340.6144-0.1070.89591.046530.4705-30.0865
705.5363-0.06770.74737.90323.49493.01020.32890.1030.2238-0.41280.058-1.7618-1.8081.4724-0.24651.0269-0.3643-0.00830.8340.06360.820111.595439.5282-24.4916
精密化 TLSグループ
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'B' and (resid 5 through 40 )
2X-RAY DIFFRACTION2chain 'B' and (resid 41 through 70 )
3X-RAY DIFFRACTION3chain 'B' and (resid 71 through 102 )
4X-RAY DIFFRACTION4chain 'B' and (resid 103 through 118 )
5X-RAY DIFFRACTION5chain 'B' and (resid 119 through 130 )
6X-RAY DIFFRACTION6chain 'B' and (resid 131 through 142 )
7X-RAY DIFFRACTION7chain 'B' and (resid 143 through 169 )
8X-RAY DIFFRACTION8chain 'A' and (resid 3 through 16 )
9X-RAY DIFFRACTION9chain 'A' and (resid 17 through 70 )
10X-RAY DIFFRACTION10chain 'A' and (resid 71 through 84 )
11X-RAY DIFFRACTION11chain 'A' and (resid 85 through 94 )
12X-RAY DIFFRACTION12chain 'A' and (resid 95 through 103 )
13X-RAY DIFFRACTION13chain 'A' and (resid 104 through 118 )
14X-RAY DIFFRACTION14chain 'A' and (resid 119 through 130 )
15X-RAY DIFFRACTION15chain 'A' and (resid 131 through 142 )
16X-RAY DIFFRACTION16chain 'A' and (resid 143 through 168 )
17X-RAY DIFFRACTION17chain 'C' and (resid 3 through 57 )
18X-RAY DIFFRACTION18chain 'C' and (resid 58 through 68 )
19X-RAY DIFFRACTION19chain 'C' and (resid 69 through 84 )
20X-RAY DIFFRACTION20chain 'C' and (resid 85 through 130 )
21X-RAY DIFFRACTION21chain 'C' and (resid 131 through 142 )
22X-RAY DIFFRACTION22chain 'C' and (resid 143 through 169 )
23X-RAY DIFFRACTION23chain 'D' and (resid 5 through 27 )
24X-RAY DIFFRACTION24chain 'D' and (resid 28 through 40 )
25X-RAY DIFFRACTION25chain 'D' and (resid 41 through 70 )
26X-RAY DIFFRACTION26chain 'D' and (resid 71 through 85 )
27X-RAY DIFFRACTION27chain 'D' and (resid 86 through 105 )
28X-RAY DIFFRACTION28chain 'D' and (resid 106 through 117 )
29X-RAY DIFFRACTION29chain 'D' and (resid 118 through 130 )
30X-RAY DIFFRACTION30chain 'D' and (resid 131 through 142 )
31X-RAY DIFFRACTION31chain 'D' and (resid 143 through 168 )
32X-RAY DIFFRACTION32chain 'E' and (resid 5 through 16 )
33X-RAY DIFFRACTION33chain 'E' and (resid 17 through 69 )
34X-RAY DIFFRACTION34chain 'E' and (resid 70 through 84 )
35X-RAY DIFFRACTION35chain 'E' and (resid 85 through 94 )
36X-RAY DIFFRACTION36chain 'E' and (resid 95 through 105 )
37X-RAY DIFFRACTION37chain 'E' and (resid 106 through 118 )
38X-RAY DIFFRACTION38chain 'E' and (resid 119 through 130 )
39X-RAY DIFFRACTION39chain 'E' and (resid 131 through 142 )
40X-RAY DIFFRACTION40chain 'E' and (resid 143 through 168 )
41X-RAY DIFFRACTION41chain 'F' and (resid 8 through 25 )
42X-RAY DIFFRACTION42chain 'F' and (resid 26 through 34 )
43X-RAY DIFFRACTION43chain 'F' and (resid 35 through 45 )
44X-RAY DIFFRACTION44chain 'F' and (resid 46 through 67 )
45X-RAY DIFFRACTION45chain 'F' and (resid 68 through 102 )
46X-RAY DIFFRACTION46chain 'F' and (resid 103 through 118 )
47X-RAY DIFFRACTION47chain 'F' and (resid 119 through 130 )
48X-RAY DIFFRACTION48chain 'F' and (resid 131 through 156 )
49X-RAY DIFFRACTION49chain 'F' and (resid 157 through 168 )
50X-RAY DIFFRACTION50chain 'G' and (resid 6 through 95 )
51X-RAY DIFFRACTION51chain 'G' and (resid 96 through 168 )
52X-RAY DIFFRACTION52chain 'H' and (resid 5 through 16 )
53X-RAY DIFFRACTION53chain 'H' and (resid 17 through 27 )
54X-RAY DIFFRACTION54chain 'H' and (resid 28 through 70 )
55X-RAY DIFFRACTION55chain 'H' and (resid 71 through 101 )
56X-RAY DIFFRACTION56chain 'H' and (resid 102 through 113 )
57X-RAY DIFFRACTION57chain 'H' and (resid 114 through 130 )
58X-RAY DIFFRACTION58chain 'H' and (resid 131 through 142 )
59X-RAY DIFFRACTION59chain 'H' and (resid 143 through 169 )
60X-RAY DIFFRACTION60chain 'I' and (resid 5 through 68 )
61X-RAY DIFFRACTION61chain 'I' and (resid 69 through 93 )
62X-RAY DIFFRACTION62chain 'I' and (resid 94 through 130 )
63X-RAY DIFFRACTION63chain 'I' and (resid 131 through 169 )
64X-RAY DIFFRACTION64chain 'J' and (resid 1 through 16 )
65X-RAY DIFFRACTION65chain 'J' and (resid 17 through 68 )
66X-RAY DIFFRACTION66chain 'J' and (resid 69 through 85 )
67X-RAY DIFFRACTION67chain 'J' and (resid 86 through 93 )
68X-RAY DIFFRACTION68chain 'J' and (resid 94 through 130 )
69X-RAY DIFFRACTION69chain 'J' and (resid 131 through 142 )
70X-RAY DIFFRACTION70chain 'J' and (resid 143 through 168 )

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万見について

-
お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbjlvh1.pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月:コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか? / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例:em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る