温度: 291 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 4.6 詳細: 3 uL hanging drop (2:1 protein:precipitant) grown over precipitant solution containing 25%PEG4000, 0.2M ammonium sulfate, and 0.1M sodium acetate pH 4.6. Protein (10 mg/mL) was in a buffer ...詳細: 3 uL hanging drop (2:1 protein:precipitant) grown over precipitant solution containing 25%PEG4000, 0.2M ammonium sulfate, and 0.1M sodium acetate pH 4.6. Protein (10 mg/mL) was in a buffer containing 25 mM Tris pH 8.0, 75 mM NaCl, and 2 mM CaCl2
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データ収集
回折
平均測定温度: 100 K / Ambient temp details: cryo cooled / Serial crystal experiment: N
構造決定の手法: 分子置換 / 解像度: 1.58→39.81 Å / Cor.coef. Fo:Fc: 0.972 / Cor.coef. Fo:Fc free: 0.96 / SU B: 1.615 / SU ML: 0.056 / 交差検証法: THROUGHOUT / ESU R: 0.075 / ESU R Free: 0.075 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.18084
2463
4.8 %
RANDOM
Rwork
0.15571
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obs
0.15695
49216
99 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK