+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 8uwb | |||||||||||||||||||||
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タイトル | Crystal structure of PP2A PPP2R1A-PPP2CA-PPP2R5E phosphatase. | |||||||||||||||||||||
要素 |
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キーワード | CELL CYCLE / PP2A / protein phosphatase / Serine/threonine-protein phosphatase 2A / B56epsilon | |||||||||||||||||||||
機能・相同性 | 機能・相同性情報 meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / regulation of microtubule binding / mitotic sister chromatid separation / MASTL Facilitates Mitotic Progression / regulation of meiotic cell cycle process involved in oocyte maturation / protein phosphatase type 2A complex / meiotic sister chromatid cohesion, centromeric / peptidyl-serine dephosphorylation ...meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / regulation of microtubule binding / mitotic sister chromatid separation / MASTL Facilitates Mitotic Progression / regulation of meiotic cell cycle process involved in oocyte maturation / protein phosphatase type 2A complex / meiotic sister chromatid cohesion, centromeric / peptidyl-serine dephosphorylation / peptidyl-threonine dephosphorylation / positive regulation of microtubule binding / Regulation of glycolysis by fructose 2,6-bisphosphate metabolism / Inhibition of replication initiation of damaged DNA by RB1/E2F1 / female meiotic nuclear division / protein antigen binding / protein phosphatase regulator activity / GABA receptor binding / APC truncation mutants have impaired AXIN binding / AXIN missense mutants destabilize the destruction complex / Truncations of AMER1 destabilize the destruction complex / Initiation of Nuclear Envelope (NE) Reformation / ERKs are inactivated / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / Beta-catenin phosphorylation cascade / Signaling by GSK3beta mutants / CTNNB1 S33 mutants aren't phosphorylated / CTNNB1 S37 mutants aren't phosphorylated / CTNNB1 S45 mutants aren't phosphorylated / CTNNB1 T41 mutants aren't phosphorylated / negative regulation of epithelial to mesenchymal transition / Disassembly of the destruction complex and recruitment of AXIN to the membrane / regulation of growth / negative regulation of glycolytic process through fructose-6-phosphate / positive regulation of NLRP3 inflammasome complex assembly / myosin phosphatase activity / CTLA4 inhibitory signaling / protein serine/threonine phosphatase activity / Platelet sensitization by LDL / protein-serine/threonine phosphatase / regulation of cell differentiation / ERK/MAPK targets / T cell homeostasis / protein phosphatase activator activity / regulation of G1/S transition of mitotic cell cycle / mesoderm development / phosphoprotein phosphatase activity / chromosome, centromeric region / DARPP-32 events / lateral plasma membrane / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Cyclin A/B1/B2 associated events during G2/M transition / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Recruitment of NuMA to mitotic centrosomes / Resolution of Sister Chromatid Cohesion / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / protein dephosphorylation / meiotic cell cycle / protein tyrosine phosphatase activity / chromosome segregation / RHO GTPases Activate Formins / response to lead ion / RAF activation / regulation of protein phosphorylation / Spry regulation of FGF signaling / Degradation of beta-catenin by the destruction complex / tau protein binding / PKR-mediated signaling / positive regulation of protein serine/threonine kinase activity / spindle pole / Negative regulation of MAPK pathway / Separation of Sister Chromatids / Cyclin D associated events in G1 / microtubule cytoskeleton / Regulation of PLK1 Activity at G2/M Transition / Regulation of TP53 Degradation / mitotic cell cycle / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / protein-containing complex assembly / intracellular signal transduction / neuron projection / membrane raft / protein heterodimerization activity / neuronal cell body / glutamatergic synapse / synapse / dendrite / signal transduction / mitochondrion / extracellular exosome / membrane / nucleus / metal ion binding 類似検索 - 分子機能 | |||||||||||||||||||||
生物種 | Homo sapiens (ヒト) | |||||||||||||||||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 3.15 Å | |||||||||||||||||||||
データ登録者 | Wachter, F. / Nowak, R.P. / Fischer, E.S. | |||||||||||||||||||||
資金援助 | 米国, 6件
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引用 | ジャーナル: J.Biol.Chem. / 年: 2024 タイトル: Structural characterization of methylation-independent PP2A assembly guides alphafold2Multimer prediction of family-wide PP2A complexes. 著者: Wachter, F. / Nowak, R.P. / Ficarro, S. / Marto, J. / Fischer, E.S. | |||||||||||||||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8uwb.cif.gz | 1 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8uwb.ent.gz | 874.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 8uwb.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 8uwb_validation.pdf.gz | 484.7 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 8uwb_full_validation.pdf.gz | 519.3 KB | 表示 | |
XML形式データ | 8uwb_validation.xml.gz | 85 KB | 表示 | |
CIF形式データ | 8uwb_validation.cif.gz | 114.6 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/uw/8uwb ftp://data.pdbj.org/pub/pdb/validation_reports/uw/8uwb | HTTPS FTP |
-関連構造データ
類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
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-リンク
-集合体
登録構造単位 |
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2 |
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単位格子 |
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-要素
#1: タンパク質 | 分子量: 68002.094 Da / 分子数: 2 / 由来タイプ: 組換発現 詳細: MDWSHPQFEKSAVDENLYFQGGGR constitutes TEV cleavable Strep II tag used in affinity purifications. 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: PPP2CA / 発現宿主: Trichoplusia ni (イラクサキンウワバ) / 参照: UniProt: P67775 #2: タンパク質 | 分子量: 54770.254 Da / 分子数: 2 / 由来タイプ: 組換発現 詳細: the N and C terminus is not visible in electron density and not modeled in. 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: PPP2R5E / 発現宿主: Trichoplusia ni (イラクサキンウワバ) / 参照: UniProt: Q16537 #3: タンパク質 | 分子量: 38420.137 Da / 分子数: 2 / 由来タイプ: 組換発現 詳細: Tev cleavable Flag tag is used for purification. Flag-TEVsite: MDYKDDDDKSAVDENLYFQGGGR 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: PPP2R1A / 発現宿主: Trichoplusia ni (イラクサキンウワバ) / 参照: UniProt: P30153 #4: 化合物 | ChemComp-MN / 研究の焦点であるリガンドがあるか | N | |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 3.22 Å3/Da / 溶媒含有率: 61.75 % |
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結晶化 | 温度: 297 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 5.77 / 詳細: 23% (w/v) PEG 20k, 0.1 M BIS-TRIS pH 5.77 |
-データ収集
回折 | 平均測定温度: 100 K / Serial crystal experiment: N |
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放射光源 | 由来: シンクロトロン / サイト: APS / ビームライン: 24-ID-C / 波長: 0.97911 Å |
検出器 | タイプ: DECTRIS EIGER X 16M / 検出器: PIXEL / 日付: 2021年3月5日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.97911 Å / 相対比: 1 |
反射 | 解像度: 3.15→128.4 Å / Num. obs: 62227 / % possible obs: 96.42 % / 冗長度: 7.4 % / Biso Wilson estimate: 107.65 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.154 / Rpim(I) all: 0.06023 / Rrim(I) all: 0.1655 / Net I/σ(I): 9.16 |
反射 シェル | 解像度: 3.15→3.263 Å / 冗長度: 0.82 % / Rmerge(I) obs: 2.011 / Mean I/σ(I) obs: 0.82 / Num. unique obs: 5795 / CC1/2: 0.397 / Rpim(I) all: 0.821 / Rrim(I) all: 2.177 / % possible all: 90.61 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 / 解像度: 3.15→128.36 Å / SU ML: 0.5847 / 交差検証法: FREE R-VALUE / σ(F): 1.96 / 位相誤差: 32.5509 立体化学のターゲット値: GeoStd + Monomer Library + CDL v1.2
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.1 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 117.22 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 3.15→128.36 Å
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拘束条件 |
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LS精密化 シェル |
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精密化 TLS | 手法: refined / Origin x: -31.6401770466 Å / Origin y: -43.4646384379 Å / Origin z: -32.2763576472 Å
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精密化 TLSグループ | Selection details: all |