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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 8ury | ||||||
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タイトル | Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome | ||||||
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![]() | RIBOSOME / transcription / translation / coupling / expressome / NusA / RfaH / RNA / RNA polymerase | ||||||
機能・相同性 | ![]() transcription antitermination factor activity, DNA binding / RNA polymerase complex / transcription elongation-coupled chromatin remodeling / submerged biofilm formation / stringent response / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / transcription antitermination factor activity, RNA binding / bacterial-type RNA polymerase core enzyme binding ...transcription antitermination factor activity, DNA binding / RNA polymerase complex / transcription elongation-coupled chromatin remodeling / submerged biofilm formation / stringent response / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / transcription antitermination factor activity, RNA binding / bacterial-type RNA polymerase core enzyme binding / cytosolic DNA-directed RNA polymerase complex / misfolded RNA binding / Group I intron splicing / RNA folding / bacterial-type flagellum-dependent cell motility / nitrate assimilation / translational termination / DnaA-L2 complex / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / assembly of large subunit precursor of preribosome / positive regulation of RNA splicing / ribosome assembly / transcription elongation factor complex / regulation of DNA-templated transcription elongation / cytosolic ribosome assembly / transcription antitermination / DNA-templated transcription initiation / cell motility / DNA-templated transcription termination / response to radiation / maintenance of translational fidelity / mRNA 5'-UTR binding / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / ribosome biogenesis / large ribosomal subunit / transferase activity / ribosome binding / response to heat / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / small ribosomal subunit rRNA binding / protein-containing complex assembly / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / intracellular iron ion homeostasis / cytoplasmic translation / tRNA binding / protein dimerization activity / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / DNA-binding transcription factor activity / ribonucleoprotein complex / response to antibiotic / nucleotide binding / mRNA binding / magnesium ion binding / DNA binding / RNA binding / zinc ion binding / metal ion binding / membrane / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() synthetic construct (人工物) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.1 Å | ||||||
![]() | Molodtsov, V. / Wang, C. / Ebright, R.H. | ||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural basis of RfaH-mediated transcription-translation coupling. 著者: Vadim Molodtsov / Chengyuan Wang / Jing Zhang / Jason T Kaelber / Gregor Blaha / Richard H Ebright / ![]() ![]() ![]() 要旨: The NusG paralog RfaH mediates bacterial transcription-translation coupling in genes that contain a DNA sequence element, termed an ops site, required for pausing RNA polymerase (RNAP) and for ...The NusG paralog RfaH mediates bacterial transcription-translation coupling in genes that contain a DNA sequence element, termed an ops site, required for pausing RNA polymerase (RNAP) and for loading RfaH onto the paused RNAP. Here, we report cryo-electron microscopy structures of transcription-translation complexes (TTCs) containing Escherichia coli RfaH. The results show that RfaH bridges RNAP and the ribosome, with the RfaH N-terminal domain interacting with RNAP and the RfaH C-terminal domain interacting with the ribosome. The results show that the distribution of translational and orientational positions of RNAP relative to the ribosome in RfaH-coupled TTCs is more restricted than in NusG-coupled TTCs because of the more restricted flexibility of the RfaH interdomain linker. The results further suggest that the structural organization of RfaH-coupled TTCs in the 'loading state', in which RNAP and RfaH are located at the ops site during formation of the TTC, is the same as the structural organization of RfaH-coupled TTCs in the 'loaded state', in which RNAP and RfaH are located at positions downstream of the ops site during function of the TTC. The results define the structural organization of RfaH-containing TTCs and set the stage for analysis of functions of RfaH during translation initiation and transcription-translation coupling. | ||||||
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CIF形式データ | ![]() | 524.8 KB | 表示 | |
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-関連構造データ
関連構造データ | ![]() 42504MC ![]() 8upoC ![]() 8uprC ![]() 8uqlC ![]() 8uqmC ![]() 8uqpC ![]() 8ur0C ![]() 8urhC ![]() 8uriC ![]() 8urxC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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集合体
登録構造単位 | ![]()
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要素
+50S ribosomal protein ... , 25種, 25分子 01239YZcfhijklmnpqrtuvwxz
-Large ribosomal subunit protein ... , 7種, 7分子 4begosy
#5: タンパク質 | 分子量: 10713.465 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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#42: タンパク質 | 分子量: 9146.540 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#45: タンパク質 | 分子量: 7155.267 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#47: タンパク質 | 分子量: 7887.117 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#55: タンパク質 | 分子量: 7181.835 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#59: タンパク質 | 分子量: 16050.606 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#65: タンパク質 | 分子量: 13159.278 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
-DNA鎖 , 2種, 2分子 56
#6: DNA鎖 | 分子量: 11786.552 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) synthetic construct (人工物) / 発現宿主: synthetic construct (人工物) |
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#7: DNA鎖 | 分子量: 11534.374 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) synthetic construct (人工物) / 発現宿主: synthetic construct (人工物) |
-RNA鎖 , 5種, 6分子 7ABDad
#8: RNA鎖 | 分子量: 14749.310 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) synthetic construct (人工物) / 発現宿主: synthetic construct (人工物) | ||||||
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#10: RNA鎖 | 分子量: 24496.617 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() #18: RNA鎖 | | 分子量: 499690.031 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() #41: RNA鎖 | | 分子量: 941635.438 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() #44: RNA鎖 | | 分子量: 38790.090 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
-DNA-directed RNA polymerase subunit ... , 4種, 5分子 AAACADAEAF
#11: タンパク質 | 分子量: 150820.875 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() 遺伝子: rpoB, groN, nitB, rif, ron, stl, stv, tabD, b3987, JW3950 発現宿主: ![]() ![]() | ||||
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#13: タンパク質 | 分子量: 36558.680 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() #14: タンパク質 | | 分子量: 155366.781 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() #15: タンパク質 | | 分子量: 10249.547 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
-Transcription ... , 2種, 2分子 ABAG
#12: タンパク質 | 分子量: 18364.217 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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#16: タンパク質 | 分子量: 54932.684 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
-30S ribosomal protein ... , 19種, 19分子 CEFGIJKLMNOQRSTUVWX
#17: タンパク質 | 分子量: 9005.472 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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#19: タンパク質 | 分子量: 9708.464 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#20: タンパク質 | 分子量: 8524.039 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#21: タンパク質 | 分子量: 26781.670 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#23: タンパク質 | 分子量: 26031.316 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#24: タンパク質 | 分子量: 23514.199 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#25: タンパク質 | 分子量: 17629.398 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#26: タンパク質 | 分子量: 12125.993 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#27: タンパク質 | 分子量: 20055.156 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#28: タンパク質 | 分子量: 14146.557 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() 遺伝子: rpsH, A5U30_004540, A8502_004035, A9X72_02245, ACN81_25460, ACU57_21020, AM464_09160, AW118_24840, AWP47_12705, B6R15_004081, B6R31_004980, BANRA_04316, BCB93_004281, BER14_25130, BF481_ ...遺伝子: rpsH, A5U30_004540, A8502_004035, A9X72_02245, ACN81_25460, ACU57_21020, AM464_09160, AW118_24840, AWP47_12705, B6R15_004081, B6R31_004980, BANRA_04316, BCB93_004281, BER14_25130, BF481_004942, BG944_001121, BGM66_003605, BJI68_08735, BJJ90_02185, BK292_20445, BK383_24705, BKL28_004555, BTB68_004368, BTQ06_10630, BvCmsKKP061_03018, BvCmsSIP010_02673, BXT93_05535, C0P57_002656, C1Q91_004916, C2121_004174, C2M16_25465, C2R31_004621, C3F40_19780, C9E67_02785, C9Z68_22575, CA593_09710, CF22_004861, CG692_20245, CG704_20930, CG831_003637, CIG67_10735, CQ986_004893, CR538_02480, CR539_21840, CR628_001354, CTR35_003618, CV83915_02792, CY655_21260, D1912_07455, D3G36_23110, D4M65_20595, D4N09_20195, D9D43_22610, D9E49_05180, D9J61_16675, DD762_23570, DIV22_15235, DNQ45_03780, DNX30_25455, DQ87_004788, DS732_24285, DTL43_20975, DU321_12025, E4K51_21515, E5H86_24500, E6D34_21735, EAI46_07500, EC95NR1_02697, ECs4171, EEP55_004504, EIA08_23980, EIMP300_17820, EIZ93_12445, EN85_004361, EPS76_07190, EPS97_20070, EWK56_25020, ExPECSC038_03652, F7F11_22700, F7N46_23985, F9407_19270, F9413_21210, F9461_25630, F9B07_24660, FEJ01_22490, FIJ20_21170, FJQ40_18330, FKO60_25660, FOI11_019235, FOI11_03945, FPI65_20330, FPS11_25405, FTV92_20690, FV293_22440, FVB16_04895, FWK02_06295, FZN31_02610, FZU14_21845, G3V95_19870, G3W53_20980, G4A38_21535, G4A47_20805, G5603_24610, G8C01_22530, G9448_17050, GAI89_24805, GAJ12_24695, GGB84_004463, GJ11_21410, GKF66_21570, GNW61_16655, GOP25_22730, GP711_23330, GP954_00920, GP965_04320, GP975_01210, GP979_01980, GQA06_03925, GQM04_10590, GQM13_25225, GQM21_11255, GQN34_23290, GQW07_21525, GRO95_20790, GRW05_09085, GRW24_04730, GRW56_00320, GRW57_03515, GTP92_23170, GUC01_21205, H0O72_19440, HCQ42_003944, HEP30_018660, HEP34_004766, HHH44_004531, HI055_004144, HIE29_005191, HJQ60_005007, HLX92_10050, HLZ50_22155, HMV95_19630, HV109_02165, HV146_21695, HV209_14690, HVV39_09290, HVW04_17510, HVW43_18645, HVY77_02150, HVZ29_19120, HX086_19430, HX136_02170, I0E88_004513, I4I37_25280, I6H00_20900, I6H02_12015, IFB95_004810, IUC12_004856, J0541_004401, J5U05_004083, JNP96_25330, KV259_004354, KV317_004326, KV371_004340, KV406_004333, KV449_004368, KV455_004331, KV463_004353, KV469_004346, KV499_004417, KV500_004304, NCTC10082_02835, NCTC10089_00498, NCTC10090_03523, NCTC10764_03859, NCTC10767_01512, NCTC11112_01040, NCTC11124_04717, NCTC11126_02767, NCTC11181_02734, NCTC11341_01893, NCTC12950_00475, NCTC13148_03445, NCTC4450_02471, NCTC7922_04046, NCTC7927_00547, NCTC7928_02520, NCTC8009_01507, NCTC8179_05938, NCTC8333_00485, NCTC8500_00329, NCTC8621_00504, NCTC8959_03265, NCTC8960_03066, NCTC8985_04978, NCTC9001_04250, NCTC9045_00561, NCTC9077_00600, NCTC9081_00913, NCTC9117_00752, NCTC9702_00533, NCTC9706_02733, NCTC9962_06793, NEP60_26315, O5851_12305, RG28_21635, SAMEA3752557_01956, TUM18780_04320, WR15_19630 発現宿主: ![]() ![]() |
#29: タンパク質 | 分子量: 14886.270 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() 遺伝子: rpsI, A5U30_002029, A6592_14035, A8499_002400, A8502_003025, A9X72_02640, AC065_15530, ACN81_18270, ACU57_14275, AM464_08780, APX88_13640, AW118_08655, AWP47_10935, B6R12_000313, B6R15_ ...遺伝子: rpsI, A5U30_002029, A6592_14035, A8499_002400, A8502_003025, A9X72_02640, AC065_15530, ACN81_18270, ACU57_14275, AM464_08780, APX88_13640, AW118_08655, AWP47_10935, B6R12_000313, B6R15_000990, B6R17_003098, B6R31_001806, B6R48_002633, B6R87_002413, BANRA_04240, BB540_06935, BER14_09340, BF481_001923, BG944_002163, BGM66_003544, BHS81_19395, BJI68_19125, BJJ90_02580, BK292_10050, BK383_16130, BKL28_000276, BMT50_12670, BMT91_11440, BR158_000189, BTB68_000438, BTQ06_23470, BUO55_000295, BvCmsKKP061_00922, BvCmsSIP010_02602, BXT93_24385, BZL69_07895, C0P57_001852, C1Q91_000916, C2121_004467, C2M16_04035, C2R31_003461, C3F40_20175, C5N07_22725, C9E67_03180, C9Z68_06055, CA593_10120, CCS08_10250, CCU84_001112, CDL36_04425, CF22_002985, CG692_13375, CG704_16430, CG831_001247, CIG67_16815, CQ986_001737, CQB02_14740, CR538_02875, CR539_21445, CR628_001957, CRM83_23300, CTR35_000744, CV83915_02871, CWS33_08570, CX938_003362, CY655_20865, D1912_21090, D3C88_18900, D3G36_08955, D4M65_13015, D4N09_15115, D6T60_21260, D9D43_03785, D9E34_11910, D9E49_09750, D9H94_03690, D9J03_05350, D9J61_01070, DD762_12930, DIV22_02780, DNQ45_08935, DNX30_11580, DS732_23890, DTL43_04485, DTL90_07760, DTM45_14705, DU321_08840, E0I52_08435, E4K51_20805, E5H86_10565, E6D34_05915, EA239_09010, EAI46_01110, EB534_RS02840, EC95NR1_02610, ECs4103, EIA08_12335, EIZ93_10150, EN85_002333, EPS76_12290, EPS97_17880, ERS085406_02965, ERS139208_02274, EWK56_02620, ExPECSC038_02741, F7F11_08870, F7N46_13330, F9413_10425, F9461_07600, F9B07_01435, FA868_08430, FEJ01_06675, FFF58_13605, FGG80_03865, FHD44_17870, FHQ97_04375, FIJ20_03860, FJQ40_07185, FKO60_06410, FN993_000631, FOI11_019605, FOI11_03575, FPI65_19955, FPS11_10475, FTV92_21060, FTV93_21255, FV293_08185, FVB16_17315, FZN31_03025, FZU14_16365, G3V95_08965, G3W53_05220, G4A38_08495, G4A47_07885, G5603_16210, G9448_17420, GAI89_03645, GAJ12_14530, GAJ26_01560, GGB84_000282, GIB53_11705, GJ11_20975, GKF52_04875, GKF66_06285, GKF89_22220, GNW61_12520, GOP14_001750, GOP25_03570, GP711_11340, GP965_13255, GP975_23890, GP979_14590, GQA06_14425, GQM04_13525, GQM13_09620, GQM17_12695, GQM21_01635, GQN34_18070, GQW07_04110, GRO95_03860, GRW05_16510, GRW24_10515, GRW56_09935, GRW57_10250, GSM54_11730, GSY44_07995, GUC01_09885, GUI33_02630, H0O53_08235, H0O72_09800, H0P11_01640, H5Z35_000289, H6Y26_003879, HCQ42_000364, HEP30_003470, HEP34_000253, HHH44_002822, HI055_000385, HIE29_003186, HJQ60_003835, HJY54_000681, HKA49_001576, HLQ92_10840, HLV18_15680, HLX92_02455, HLZ50_05640, HMV95_21920, HMW38_00255, HV109_02535, HV146_21315, HV209_15060, HVV39_08880, HVW04_17925, HVW43_19020, HVY77_02530, HVZ29_18700, HVZ30_02410, HVZ71_02705, HX136_02545, I6H00_21285, I6H02_11635, IFB95_002590, IUD45_003198, J0541_003264, J4S20_002626, J5U05_001240, J7T50_001183, JFD_02469, JFD_04155, JNP96_24960, KQ660_11425, NCTC10082_02912, NCTC10089_00573, NCTC10090_03603, NCTC10418_00792, NCTC10487_05043, NCTC10764_03943, NCTC10767_01601, NCTC10865_00738, NCTC10974_00628, NCTC11112_00948, NCTC11126_02865, NCTC11127_03151, NCTC11181_02813, NCTC11341_01809, NCTC12950_00560, NCTC13127_00868, NCTC4450_02548, NCTC7922_03943, NCTC7927_00632, NCTC7928_02411, NCTC8179_06030, NCTC8450_05022, NCTC8500_00425, NCTC8621_00591, NCTC8622_00406, NCTC8959_03190, NCTC8960_03154, NCTC9001_05559, NCTC9044_01272, NCTC9045_00641, NCTC9050_03684, NCTC9071_00556, NCTC9073_00293, NCTC9075_00824, NCTC9077_00687, NCTC9081_01013, NCTC9111_00940, NCTC9117_00832, NCTC9702_00613, NCTC9706_02809, NCTC9775_04087, NCTC9777_01975, NCTC9962_00338, NEP60_07135, OS110_19195, RG28_21240, SAMEA3472044_04260, SAMEA3472056_03130, SAMEA3752557_01816, SAMEA3753106_02430, TUM18780_04980, WR15_24270 発現宿主: ![]() ![]() |
#31: タンパク質 | 分子量: 12487.200 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#32: タンパク質 | 分子量: 13768.157 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#33: タンパク質 | 分子量: 11606.560 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#34: タンパク質 | 分子量: 10290.816 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#35: タンパク質 | 分子量: 9207.572 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
#36: タンパク質 | 分子量: 9724.491 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() 遺伝子: rpsQ, A5U30_004545, A6592_25760, A8499_003325, A8502_004030, A9X72_02220, AC065_15970, ACN81_25485, ACU57_20995, AM464_09185, APX88_16110, AW118_24865, AWP47_12680, B6R12_002814, B6R15_ ...遺伝子: rpsQ, A5U30_004545, A6592_25760, A8499_003325, A8502_004030, A9X72_02220, AC065_15970, ACN81_25485, ACU57_20995, AM464_09185, APX88_16110, AW118_24865, AWP47_12680, B6R12_002814, B6R15_004076, B6R17_004949, B6R31_004975, B6R48_005042, B6R87_004952, BANRA_04321, BB540_01705, BER14_25155, BF481_004937, BG944_001126, BGM66_003600, BHS81_19800, BJI68_08710, BJJ90_02160, BK292_20470, BK383_24680, BKL28_004550, BMT50_12250, BMT91_20035, BR158_003783, BTB68_004363, BTQ06_10655, BUO55_004704, BvCmsKKP061_03023, BvCmsSIP010_02678, BXT93_05510, BZL69_25995, C0P57_002661, C1Q91_004911, C2121_004169, C2M16_25440, C2R31_004626, C3F40_19755, C5N07_23525, C9E67_02760, C9Z68_22600, CA593_09685, CCS08_00180, CCU84_002909, CDL36_25545, CF22_004856, CG692_20220, CG704_20905, CG831_003642, CIG67_10710, CQ986_004888, CQB02_00675, CR538_02455, CR539_21865, CR628_001359, CRM83_22880, CTR35_003623, CV83915_02787, CWS33_24905, CX938_003824, CY655_21285, D1912_22345, D3C88_12855, D3G36_23085, D4M65_20620, D4N09_20220, D6T60_05740, D9D43_22585, D9E34_23560, D9E49_05205, D9H94_20785, D9J03_20100, D9J61_16700, DD762_23595, DIV22_15210, DNQ45_03805, DNX30_25430, DS732_24310, DTL43_20950, DTL90_24740, DTM45_24505, DU321_12050, E0I52_20190, E4K51_21540, E5H86_24475, E6D34_21710, EA239_22840, EAI46_07525, EB534_RS01750, EC95NR1_02702, ECs4176, EIA08_24005, EIZ93_12420, EN85_004356, EPS76_07165, EPS97_20045, ERS085406_04397, ERS139208_04345, EWK56_25045, ExPECSC038_03647, F3P10_05520, F7F11_22675, F7N46_23960, F9413_21185, F9461_25605, F9B07_24635, FA868_22795, FEJ01_22465, FFF58_25405, FGG80_25085, FHD44_21315, FHQ97_20070, FIJ20_21145, FJQ40_18355, FKO60_25685, FN993_004702, FOI11_019210, FOI11_03970, FPI65_20355, FPS11_25380, FTV92_20665, FTV93_20850, FV293_22415, FVB16_04920, FWK02_06320, FZN31_02585, FZU14_21870, G3V95_19895, G3W53_21005, G4A38_21560, G4A47_20830, G5603_24635, G9448_17025, GAI89_24780, GAJ12_24670, GAJ26_22060, GGB84_004458, GIB53_19350, GJ11_21435, GKF52_21290, GKF66_21595, GKF89_18520, GNW61_16680, GOP14_004282, GOP25_22755, GP711_23355, GP954_00895, GP965_04345, GP975_01235, GP979_01955, GQA06_03900, GQM04_10615, GQM13_25250, GQM17_19965, GQM21_11280, GQN34_23315, GQW07_21500, GRO95_20765, GRW05_09110, GRW24_04705, GRW56_00345, GRW57_03490, GSM54_23280, GSY44_20050, GUC01_21230, GUI33_20095, H0O53_20780, H0O72_19465, H0P11_20875, H5Z35_004780, H6Y26_003173, HCQ42_003939, HEP30_018685, HEP34_004761, HHH44_004526, HI055_004149, HIE29_005196, HJQ60_005002, HJY54_004744, HKA49_005026, HLQ92_22550, HLV18_23290, HLX92_10025, HLZ50_22130, HMV95_19655, HMW38_23155, HV109_02140, HV146_21720, HV209_14665, HVV39_09315, HVW04_17485, HVW43_18620, HVY77_02125, HVZ29_19145, HVZ30_02005, HVZ71_02310, HX136_02145, I6H00_20875, I6H02_12040, IFB95_004815, IUD45_003549, J0541_004406, J4S20_004793, J5U05_004078, J7T50_001462, JFD_01893, JFD_03722, JNP96_25355, KQ660_11820, NCTC10082_02830, NCTC10089_00493, NCTC10090_03518, NCTC10418_00682, NCTC10429_01085, NCTC10487_00436, NCTC10764_03854, NCTC10767_01507, NCTC10865_00650, NCTC10974_00540, NCTC11112_01045, NCTC11127_03232, NCTC11181_02729, NCTC11341_01898, NCTC12950_00470, NCTC13127_00777, NCTC4450_02466, NCTC7922_04051, NCTC7927_00542, NCTC7928_02526, NCTC8009_01502, NCTC8179_05933, NCTC8333_00480, NCTC8450_04928, NCTC8500_00325, NCTC8621_00499, NCTC8622_00515, NCTC8959_03270, NCTC8960_03061, NCTC8985_04973, NCTC9044_01367, NCTC9045_00556, NCTC9050_03602, NCTC9071_00474, NCTC9073_00388, NCTC9075_00715, NCTC9077_00595, NCTC9081_00908, NCTC9111_00858, NCTC9117_00747, NCTC9702_00528, NCTC9706_02728, NCTC9775_03994, NCTC9777_01882, NCTC9962_06788, NEP60_26340, OS110_25165, RG28_21660, SAMEA3472044_01153, SAMEA3472056_05420, SAMEA3752557_01961, SAMEA3753106_04625, TUM18780_04270, WR15_19655 発現宿主: ![]() ![]() |
#37: タンパク質 | 分子量: 10455.355 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() 遺伝子: rpsS, rpsS_1, A5U30_004550, A8502_004025, A9X72_02195, ACN81_25510, ACU57_20970, AM464_09210, AW118_24890, AWP47_12655, B6R15_004071, B6R31_004970, BANRA_04326, BCB93_004291, BER14_25180, ...遺伝子: rpsS, rpsS_1, A5U30_004550, A8502_004025, A9X72_02195, ACN81_25510, ACU57_20970, AM464_09210, AW118_24890, AWP47_12655, B6R15_004071, B6R31_004970, BANRA_04326, BCB93_004291, BER14_25180, BF481_004932, BG944_001131, BGM66_003595, BJI68_08685, BJJ90_02135, BK292_20495, BK383_24655, BKL28_004545, BTB68_004358, BTQ06_10680, BvCmsKKP061_03028, BvCmsSIP010_02683, BXT93_05485, C0P57_002666, C1Q91_004906, C2121_004164, C2M16_25415, C2R31_004631, C3F40_19730, C9E67_02735, C9Z68_22625, CA593_09660, CF22_004851, CG692_20195, CG704_20880, CG831_003647, CIG67_10685, CQ986_004883, CR538_02430, CR539_21890, CR628_001364, CTR35_003628, CY655_21310, D1912_22370, D3G36_23060, D4M65_20645, D4N09_20245, D9D43_22560, D9E49_05230, D9J61_16725, DD762_23620, DIV22_15185, DNQ45_03830, DNX30_25405, DQ87_004778, DTL43_20925, DU321_12075, E4K51_21565, E5H86_24450, E6D34_21685, EAI46_07550, EC95NR1_02707, ECs4181, EEP55_004494, EIA08_24030, EIZ93_12395, EN85_004351, EPS76_07140, EPS97_20020, EWK56_25070, ExPECSC038_03642, F7F11_22650, F7N46_23935, F9407_19220, F9413_21160, F9461_25580, F9B07_24610, FEJ01_22440, FIJ20_21120, FJQ40_18380, FKO60_25710, FOI11_019185, FOI11_03995, FPI65_20380, FPS11_25355, FTV92_20640, FTV93_20825, FV293_22390, FVB16_04945, FWK02_12640, FZN31_02560, FZU14_21895, G3V95_19920, G3W53_21030, G4A38_21585, G4A47_20855, G5603_24660, G8C01_22480, G9448_17000, GAI89_24755, GAJ12_24645, GGB84_004453, GJ11_21460, GKF66_21620, GNW61_16705, GOP25_22780, GP711_23380, GP954_00870, GP965_04370, GP975_01260, GP979_01930, GQA06_03875, GQM04_10640, GQM13_25275, GQM21_11305, GQN34_23340, GQW07_21475, GRO95_20740, GRW05_09135, GRW24_04680, GRW56_00370, GRW57_03465, GTP92_23120, GUC01_21255, H0O72_19490, HCQ42_003934, HEP30_018710, HEP34_004756, HHH44_004521, HI055_004154, HIE29_005201, HJQ60_004997, HLX92_10000, HLZ50_22105, HMV95_19680, HV109_02115, HV146_21745, HV209_14640, HVV39_09340, HVW04_17460, HVW43_18595, HVY77_02100, HVZ29_19170, HX086_19480, HX136_02120, I0E88_004503, I4I37_21030, I6H00_20850, I6H02_12065, IFB95_004820, IUC12_004846, J0541_004411, J5U05_004073, JNP96_25380, KV259_004344, KV317_004316, KV371_004330, KV406_004323, KV449_004358, KV455_004321, KV463_004343, KV469_004336, KV499_004407, KV500_004294, NCTC10082_02825, NCTC10089_00488, NCTC10090_03513, NCTC10429_01079, NCTC10764_03849, NCTC10767_01502, NCTC10865_00644, NCTC10974_00535, NCTC11112_01049, NCTC11126_02756, NCTC11181_02724, NCTC11341_01903, NCTC12950_00465, NCTC4450_02461, NCTC7927_00537, NCTC7928_02531, NCTC8179_05927, NCTC8333_00475, NCTC8500_00320, NCTC8621_00494, NCTC8622_00520, NCTC8959_03275, NCTC8960_03056, NCTC8985_04967, NCTC9001_04240, NCTC9044_01374, NCTC9045_00551, NCTC9077_00590, NCTC9081_00903, NCTC9117_00742, NCTC9702_00523, NCTC9706_02723, NCTC9962_06783, NEP60_26365, O5851_12255, RG28_21685, SAMEA3752557_01966, TUM18780_04220, WR15_19680 発現宿主: ![]() ![]() |
#38: タンパク質 | 分子量: 13128.467 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
-Small ribosomal subunit protein ... , 2種, 2分子 HP
#22: タンパク質 | 分子量: 61238.887 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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#30: タンパク質 | 分子量: 11254.041 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
-非ポリマー , 2種, 3分子 


#67: 化合物 | ChemComp-MG / |
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#68: 化合物 |
-詳細
研究の焦点であるリガンドがあるか | N |
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Has protein modification | Y |
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome タイプ: COMPLEX / Entity ID: #1-#66 / 由来: RECOMBINANT |
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由来(天然) | 生物種: ![]() ![]() |
由来(組換発現) | 生物種: ![]() ![]() |
緩衝液 | pH: 7.6 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2500 nm / 最小 デフォーカス(公称値): 800 nm |
撮影 | 電子線照射量: 45 e/Å2 フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) |
-
解析
CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3次元再構成 | 解像度: 3.1 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 12096 / 対称性のタイプ: POINT |