+Open data
-Basic information
Entry | Database: PDB / ID: 8uhw | ||||||
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Title | The structure of the Clostridium thermocellum AdhE spirosome | ||||||
Components | Aldehyde-alcohol dehydrogenase | ||||||
Keywords | OXIDOREDUCTASE / Aldehyde-alcohol dehydrogenase / Complex / Spirosome | ||||||
Function / homology | Function and homology information methylglyoxal reductase (NADPH) (acetol producing) activity / butanol dehydrogenase (NAD+) activity / acetaldehyde dehydrogenase (acetylating) activity / alcohol metabolic process / alcohol dehydrogenase (NADP+) activity / carbon utilization / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | Acetivibrio thermocellus DSM 1313 (bacteria) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.28 Å | ||||||
Authors | Ziegler, S.J. / Gruber, J.N. | ||||||
Funding support | United States, 1items
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Citation | Journal: Elife / Year: 2024 Title: Structural characterization and dynamics of AdhE ultrastructures from Clostridium thermocellum: A containment strategy for toxic intermediates Authors: Ziegler, S. / Knott, B. / Gruber, J. / Hengge, N. / Xu, Q. / Olson, D. / Romero, E. / Joubert, L. / Bomble, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8uhw.cif.gz | 695 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8uhw.ent.gz | 557.3 KB | Display | PDB format |
PDBx/mmJSON format | 8uhw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uh/8uhw ftp://data.pdbj.org/pub/pdb/validation_reports/uh/8uhw | HTTPS FTP |
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-Related structure data
Related structure data | 42284MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 97938.680 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acetivibrio thermocellus DSM 1313 (bacteria) Gene: adhE / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0H3W5U9 #2: Chemical | ChemComp-FE / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Homo-oligomer complex of AdhE / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT | |||||||||||||||
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Molecular weight | Experimental value: NO | |||||||||||||||
Source (natural) | Organism: Acetivibrio thermocellus DSM 1313 (bacteria) | |||||||||||||||
Source (recombinant) | Organism: Escherichia coli (E. coli) | |||||||||||||||
Buffer solution | pH: 7.5 | |||||||||||||||
Buffer component |
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Specimen | Conc.: 0.4 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | |||||||||||||||
Specimen support | Grid material: COPPER / Grid mesh size: 400 divisions/in. / Grid type: C-flat-1.2/1.3 | |||||||||||||||
Vitrification | Instrument: GATAN CRYOPLUNGE 3 / Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 3000 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 60 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) |
-Processing
EM software | Name: RELION / Version: 3.1 / Category: particle selection |
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CTF correction | Type: NONE |
Particle selection | Num. of particles selected: 504494 |
Symmetry | Point symmetry: C1 (asymmetric) |
3D reconstruction | Resolution: 3.28 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 90912 / Algorithm: BACK PROJECTION / Num. of class averages: 1 / Symmetry type: POINT |
Atomic model building | Protocol: RIGID BODY FIT |