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- PDB-8uca: Formation of I2+III2 supercomplex rescues respiratory chain defects -

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Basic information

Entry
Database: PDB / ID: 8uca
TitleFormation of I2+III2 supercomplex rescues respiratory chain defects
Components
  • (Cytochrome b-c1 complex subunit ...) x 9
  • (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit ...) x 12
  • (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit ...) x 11
  • (NADH dehydrogenase [ubiquinone] 1 subunit ...) x 2
  • (NADH dehydrogenase [ubiquinone] flavoprotein ...) x 3
  • (NADH dehydrogenase [ubiquinone] iron-sulfur protein ...) x 7
  • (NADH-ubiquinone oxidoreductase chain ...) x 7
  • Acyl carrier protein, mitochondrial
  • Cytochrome b
  • Cytochrome c1, heme protein, mitochondrial
  • NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
KeywordsELECTRON TRANSPORT / Translocase / Super complex XL (CI2+CIII2)
Function / homology
Function and homology information


Complex III assembly / subthalamus development / pons development / Mitochondrial protein import / response to injury involved in regulation of muscle adaptation / mesenchymal stem cell proliferation / reproductive system development / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / cerebellar Purkinje cell layer development ...Complex III assembly / subthalamus development / pons development / Mitochondrial protein import / response to injury involved in regulation of muscle adaptation / mesenchymal stem cell proliferation / reproductive system development / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / cerebellar Purkinje cell layer development / Complex I biogenesis / RHOG GTPase cycle / Respiratory electron transport / response to mercury ion / protein insertion into mitochondrial inner membrane / mesenchymal stem cell differentiation / circulatory system development / pyramidal neuron development / blastocyst hatching / thalamus development / mitochondrial respiratory chain complex III assembly / respiratory system process / psychomotor behavior / cellular respiration / Mitochondrial protein degradation / cellular response to oxygen levels / respiratory chain complex / stem cell division / response to light intensity / mitochondrial [2Fe-2S] assembly complex / iron-sulfur cluster assembly complex / mitochondrial large ribosomal subunit binding / response to copper ion / gliogenesis / response to alkaloid / respiratory chain complex III / negative regulation of non-canonical NF-kappaB signal transduction / response to glucagon / cardiac muscle tissue development / neural precursor cell proliferation / [2Fe-2S] cluster assembly / quinol-cytochrome-c reductase / positive regulation of mitochondrial membrane potential / adult walking behavior / cellular response to glucocorticoid stimulus / oxygen sensor activity / quinol-cytochrome-c reductase activity / response to hydroperoxide / mitochondrial electron transport, ubiquinol to cytochrome c / hypothalamus development / iron-sulfur cluster assembly / positive regulation of ATP biosynthetic process / midbrain development / response to cobalamin / electron transport coupled proton transport / dopamine metabolic process / adult behavior / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / positive regulation of execution phase of apoptosis / mitochondrial electron transport, NADH to ubiquinone / proton motive force-driven mitochondrial ATP synthesis / NADH dehydrogenase activity / respiratory chain complex I / response to hyperoxia / NADH dehydrogenase (ubiquinone) activity / animal organ regeneration / neuron development / quinone binding / cellular response to interferon-beta / response to cadmium ion / ATP synthesis coupled electron transport / cellular response to retinoic acid / extrinsic apoptotic signaling pathway / negative regulation of reactive oxygen species biosynthetic process / tricarboxylic acid cycle / muscle contraction / response to hormone / DNA damage response, signal transduction by p53 class mediator / Neutrophil degranulation / visual perception / cerebellum development / reactive oxygen species metabolic process / aerobic respiration / regulation of mitochondrial membrane potential / hippocampus development / kidney development / respiratory electron transport chain / response to activity / neurogenesis / fatty acid metabolic process / mitochondrion organization / response to nicotine / sensory perception of sound / response to cocaine / monooxygenase activity / electron transport chain / mitochondrial membrane / : / brain development
Similarity search - Function
Cytochrome b-c1 complex subunit 10 / Single alpha-helix domain superfamily / Ubiquinol-cytochrome C reductase complex, 6.4kD protein / NmrA-like domain / NmrA-like family / Ubiquinol-cytochrome c reductase 8kDa, N-terminal / Ubiquinol-cytochrome c reductase 8 kDa, N-terminal / Globular protein, non-globular alpha/beta subunit / Cytochrome b-c1 complex, subunit 6 / Complex1_LYR-like ...Cytochrome b-c1 complex subunit 10 / Single alpha-helix domain superfamily / Ubiquinol-cytochrome C reductase complex, 6.4kD protein / NmrA-like domain / NmrA-like family / Ubiquinol-cytochrome c reductase 8kDa, N-terminal / Ubiquinol-cytochrome c reductase 8 kDa, N-terminal / Globular protein, non-globular alpha/beta subunit / Cytochrome b-c1 complex, subunit 6 / Complex1_LYR-like / Cytochrome b-c1 complex subunit 8 / UcrQ family / Cytochrome bc1 complex subunit Rieske, transmembrane domain superfamily / Cytochrome b-c1 complex subunit 7 / Cytochrome b-c1 complex subunit 7 superfamily / Ubiquinol-cytochrome C reductase complex 14kD subunit / Cytochrome b-c1 complex subunit 8 superfamily / Cytochrome b-c1 complex subunit Rieske, transmembrane domain / Ubiquinol cytochrome reductase transmembrane region / Ubiquinol-cytochrome C reductase hinge domain / Ubiquinol-cytochrome C reductase hinge domain superfamily / Ubiquinol-cytochrome C reductase hinge protein / Cytochrome b-c1 complex subunit 9 / Cytochrome b-c1 complex subunit 9 superfamily / Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like / Cytochrome c1, transmembrane anchor, C-terminal / : / Cytochrome b / Ubiquinol-cytochrome c reductase, iron-sulphur subunit / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / : / Cytochrome c1 / Cytochrome C1 family / : / Cytochrome b/b6, C-terminal / Cytochrome b(C-terminal)/b6/petD / Cytochrome b/b6 C-terminal region profile. / Peptidase M16, zinc-binding site / Insulinase family, zinc-binding region signature. / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / Cytochrome b/b6, C-terminal domain superfamily / Cytochrome b/b6/petB / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / Tim17/Tim22/Tim23/Pmp24 family / Rieske iron-sulphur protein, C-terminal / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / Cytochrome b/b6, N-terminal / Cytochrome b/b6-like domain superfamily / Cytochrome b/b6 N-terminal region profile. / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH dehydrogenase 1 beta subcomplex subunit 2 / Di-haem cytochrome, transmembrane / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / : / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / : / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / Rieske iron-sulphur protein / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / Peptidase M16, C-terminal / Zinc finger, CHCC-type / Zinc-finger domain / Peptidase M16 inactive domain / Peptidase M16, N-terminal / Insulinase (Peptidase family M16) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family
Similarity search - Domain/homology
2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE / Chem-EHZ / FE2/S2 (INORGANIC) CLUSTER / FLAVIN MONONUCLEOTIDE / HEME C / PROTOPORPHYRIN IX CONTAINING FE / Chem-NDP / IRON/SULFUR CLUSTER / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial ...2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE / Chem-EHZ / FE2/S2 (INORGANIC) CLUSTER / FLAVIN MONONUCLEOTIDE / HEME C / PROTOPORPHYRIN IX CONTAINING FE / Chem-NDP / IRON/SULFUR CLUSTER / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / Cytochrome b / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / Cytochrome b-c1 complex subunit 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH-ubiquinone oxidoreductase chain 1 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / Cytochrome b-c1 complex subunit 9 / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / Cytochrome b-c1 complex subunit 10 / NADH dehydrogenase [ubiquinone] 1 subunit C2 / Cytochrome b-c1 complex subunit 8 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / Cytochrome b-c1 complex subunit 7 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / Acyl carrier protein, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / Cytochrome b-c1 complex subunit Rieske, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / Cytochrome b-c1 complex subunit 1, mitochondrial / Cytochrome c1, heme protein, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / Cytochrome b-c1 complex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.7 Å
AuthorsLetts, J.A. / Padavannil, A.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM137929 United States
CitationJournal: Cell Metab / Year: 2025
Title: Formation of I+III supercomplex rescues respiratory chain defects.
Authors: Chao Liang / Abhilash Padavannil / Shan Zhang / Sheryl Beh / David R L Robinson / Jana Meisterknecht / Alfredo Cabrera-Orefice / Timothy R Koves / Chika Watanabe / Miyuki Watanabe / María ...Authors: Chao Liang / Abhilash Padavannil / Shan Zhang / Sheryl Beh / David R L Robinson / Jana Meisterknecht / Alfredo Cabrera-Orefice / Timothy R Koves / Chika Watanabe / Miyuki Watanabe / María Illescas / Radiance Lim / Jordan M Johnson / Shuxun Ren / Ya-Jun Wu / Dennis Kappei / Anna Maria Ghelli / Katsuhiko Funai / Hitoshi Osaka / Deborah Muoio / Cristina Ugalde / Ilka Wittig / David A Stroud / James A Letts / Lena Ho /
Abstract: Mitochondrial electron transport chain (ETC) complexes partition between free complexes and quaternary assemblies known as supercomplexes (SCs). However, the physiological requirement for SCs and the ...Mitochondrial electron transport chain (ETC) complexes partition between free complexes and quaternary assemblies known as supercomplexes (SCs). However, the physiological requirement for SCs and the mechanisms regulating their formation remain controversial. Here, we show that genetic perturbations in mammalian ETC complex III (CIII) biogenesis stimulate the formation of a specialized extra-large SC (SC-XL) with a structure of I+III, resolved at 3.7 Å by cryoelectron microscopy (cryo-EM). SC-XL formation increases mitochondrial cristae density, reduces CIII reactive oxygen species (ROS), and sustains normal respiration despite a 70% reduction in CIII activity, effectively rescuing CIII deficiency. Consequently, inhibiting SC-XL formation in CIII mutants using the Uqcrc1 contact site mutation leads to respiratory decompensation. Lastly, SC-XL formation promotes fatty acid oxidation (FAO) and protects against ischemic heart failure in mice. Our study uncovers an unexpected plasticity in the mammalian ETC, where structural adaptations mitigate intrinsic perturbations, and suggests that manipulating SC-XL formation is a potential therapeutic strategy for mitochondrial dysfunction.
History
DepositionSep 26, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 15, 2025Provider: repository / Type: Initial release
Revision 1.1Jan 22, 2025Group: Data collection / Database references / Category: citation / citation_author / em_admin
Item: _citation.pdbx_database_id_PubMed / _citation.title ..._citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _em_admin.last_update
Revision 1.2Feb 19, 2025Group: Data collection / Database references / Category: citation / em_admin
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _em_admin.last_update

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
3: NADH-ubiquinone oxidoreductase chain 3
S3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
S2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
1: NADH-ubiquinone oxidoreductase chain 1
6: NADH-ubiquinone oxidoreductase chain 6
4L: NADH-ubiquinone oxidoreductase chain 4L
5: NADH-ubiquinone oxidoreductase chain 5
4: NADH-ubiquinone oxidoreductase chain 4
2: NADH-ubiquinone oxidoreductase chain 2
AL: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
A9: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
S4: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
S6: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
A2: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
AB: Acyl carrier protein, mitochondrial
AC: Acyl carrier protein, mitochondrial
A5: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
A6: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
A8: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
AM: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
AO: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
A1: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
A3: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
C1: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
C2: NADH dehydrogenase [ubiquinone] 1 subunit C2
S5: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
B1: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
BM: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
B5: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
B6: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
B2: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial
B3: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
B8: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
B4: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
B9: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
B7: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
BL: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
AN: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
A7: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
V3: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
3a: NADH-ubiquinone oxidoreductase chain 3
s3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
s2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
1a: NADH-ubiquinone oxidoreductase chain 1
6a: NADH-ubiquinone oxidoreductase chain 6
4l: NADH-ubiquinone oxidoreductase chain 4L
5a: NADH-ubiquinone oxidoreductase chain 5
4a: NADH-ubiquinone oxidoreductase chain 4
2a: NADH-ubiquinone oxidoreductase chain 2
al: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
a9: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
s4: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
s6: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
a2: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
ab: Acyl carrier protein, mitochondrial
ac: Acyl carrier protein, mitochondrial
a5: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
a6: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
a8: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
am: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
ao: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
a1: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
a3: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
c1: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
c2: NADH dehydrogenase [ubiquinone] 1 subunit C2
s5: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
b1: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
bm: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
b5: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
b6: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
b2: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial
b3: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
b8: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
b4: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
b9: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
b7: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
bl: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
an: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
a7: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
v3: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
3A: Cytochrome b-c1 complex subunit 1, mitochondrial
3B: Cytochrome b-c1 complex subunit 2, mitochondrial
3C: Cytochrome b
3D: Cytochrome c1, heme protein, mitochondrial
3E: Cytochrome b-c1 complex subunit 9
3F: Cytochrome b-c1 complex subunit 7
3G: Cytochrome b-c1 complex subunit 8
3H: Cytochrome b-c1 complex subunit 6, mitochondrial
3J: Cytochrome b-c1 complex subunit 9
3K: Cytochrome b-c1 complex subunit 10
3L: Cytochrome b-c1 complex subunit 1, mitochondrial
3M: Cytochrome b-c1 complex subunit 2, mitochondrial
3N: Cytochrome b
3O: Cytochrome c1, heme protein, mitochondrial
3P: Cytochrome b-c1 complex subunit 9
3Q: Cytochrome b-c1 complex subunit 7
3R: Cytochrome b-c1 complex subunit 8
3S: Cytochrome b-c1 complex subunit 6, mitochondrial
3T: Cytochrome b-c1 complex subunit Rieske, mitochondrial
3U: Cytochrome b-c1 complex subunit 9
3V: Cytochrome b-c1 complex subunit 10
V1: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
V2: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
S1: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
S7: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
S8: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
v1: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
v2: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
s1: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
s7: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
s8: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,577,848147
Polymers2,563,286111
Non-polymers14,56236
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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NADH-ubiquinone oxidoreductase chain ... , 7 types, 14 molecules 33a11a66a4L4l55a44a22a

#1: Protein NADH-ubiquinone oxidoreductase chain 3


Mass: 13223.775 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: mt-Nd3 / Production host: Mus musculus (house mouse) / References: UniProt: P03899
#4: Protein NADH-ubiquinone oxidoreductase chain 1


Mass: 36105.027 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: mt-Nd1, ND1, Nd1 / Production host: Mus musculus (house mouse)
References: UniProt: Q4JFN6, NADH:ubiquinone reductase (H+-translocating)
#5: Protein NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase subunit 6


Mass: 18628.090 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Mtnd6, mt-Nd6, Nd6 / Production host: Mus musculus (house mouse)
References: UniProt: P03925, NADH:ubiquinone reductase (H+-translocating)
#6: Protein NADH-ubiquinone oxidoreductase chain 4L / NADH dehydrogenase subunit 4L


Mass: 10609.619 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Mtnd4l, mt-Nd4l, Nd4l / Production host: Mus musculus (house mouse)
References: UniProt: P03903, NADH:ubiquinone reductase (H+-translocating)
#7: Protein NADH-ubiquinone oxidoreductase chain 5 / NADH dehydrogenase subunit 5


Mass: 68519.289 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Mtnd5, mt-Nd5, Nd5 / Production host: Mus musculus (house mouse)
References: UniProt: P03921, NADH:ubiquinone reductase (H+-translocating)
#8: Protein NADH-ubiquinone oxidoreductase chain 4 / NADH dehydrogenase subunit 4


Mass: 51915.535 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Mtnd4, mt-Nd4, Nd4 / Production host: Mus musculus (house mouse)
References: UniProt: P03911, NADH:ubiquinone reductase (H+-translocating)
#9: Protein NADH-ubiquinone oxidoreductase chain 2 / NADH dehydrogenase subunit 2


Mass: 38772.219 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: mt-Nd2, Nd2 / Production host: Mus musculus (house mouse)
References: UniProt: P03893, NADH:ubiquinone reductase (H+-translocating)

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NADH dehydrogenase [ubiquinone] iron-sulfur protein ... , 7 types, 14 molecules S3s3S2s2S4s4S6s6S5s5S7s7S8s8

#2: Protein NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / Complex I-30kD / CI-30kD / NADH-ubiquinone oxidoreductase 30 kDa subunit


Mass: 30191.307 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufs3 / Production host: Mus musculus (house mouse)
References: UniProt: Q9DCT2, NADH:ubiquinone reductase (H+-translocating)
#3: Protein NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / Complex I-49kD / CI-49kD / NADH-ubiquinone oxidoreductase 49 kDa subunit


Mass: 52720.602 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufs2 / Production host: Mus musculus (house mouse)
References: UniProt: Q91WD5, NADH:ubiquinone reductase (H+-translocating)
#12: Protein NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / Complex I-18 kDa / CI-18 kDa / Complex I-AQDQ / CI-AQDQ / NADH-ubiquinone oxidoreductase 18 kDa subunit


Mass: 19814.725 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufs4 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CXZ1
#13: Protein NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / Complex I-13kD-A / CI-13kD-A / NADH-ubiquinone oxidoreductase 13 kDa-A subunit


Mass: 13041.828 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufs6, Ip13 / Production host: Mus musculus (house mouse) / References: UniProt: P52503
#25: Protein NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / Complex I-15 kDa / CI-15 kDa / NADH-ubiquinone oxidoreductase 15 kDa subunit


Mass: 12675.772 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufs5 / Production host: Mus musculus (house mouse) / References: UniProt: Q99LY9
#54: Protein NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / Complex I-20kD / CI-20kD / NADH-ubiquinone oxidoreductase 20 kDa subunit


Mass: 24715.912 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufs7 / Production host: Mus musculus (house mouse)
References: UniProt: Q9DC70, NADH:ubiquinone reductase (H+-translocating)
#55: Protein NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / Complex I-23kD / CI-23kD / NADH-ubiquinone oxidoreductase 23 kDa subunit


Mass: 24068.355 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufs8 / Production host: Mus musculus (house mouse)
References: UniProt: Q8K3J1, NADH:ubiquinone reductase (H+-translocating)

-
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit ... , 12 types, 24 molecules ALalA9a9A2a2A5a5A6a6A8a8AMamAOaoA1a1A3a3ANanA7a7

#10: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / Complex I-42kD / CI-42kD / NADH-ubiquinone oxidoreductase 42 kDa subunit


Mass: 40657.375 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa10 / Production host: Mus musculus (house mouse) / References: UniProt: Q99LC3
#11: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial


Mass: 42588.129 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa9 / Production host: Mus musculus (house mouse) / References: UniProt: Q9DC69
#14: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / Complex I-B8 / CI-B8 / NADH-ubiquinone oxidoreductase B8 subunit


Mass: 10932.675 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa2 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CQ75
#16: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / Complex I subunit B13 / Complex I-13kD-B / CI-13kD-B / NADH-ubiquinone oxidoreductase 13 kDa-B subunit


Mass: 13380.719 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa5 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CPP6
#17: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / Complex I-B14 / CI-B14 / NADH-ubiquinone oxidoreductase B14 subunit


Mass: 15311.858 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa6 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CQZ5
#18: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / Complex I-19kD / CI-19kD / Complex I-PGIV / CI-PGIV / NADH-ubiquinone oxidoreductase 19 kDa subunit


Mass: 20025.127 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa8 / Production host: Mus musculus (house mouse) / References: UniProt: Q9DCJ5
#19: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11


Mass: 14999.220 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa11 / Production host: Mus musculus (house mouse) / References: UniProt: G5E814
#20: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / Cell death regulatory protein GRIM-19 / Complex I-B16.6 / CI-B16.6 / Gene associated with retinoic ...Cell death regulatory protein GRIM-19 / Complex I-B16.6 / CI-B16.6 / Gene associated with retinoic and interferon-induced mortality 19 protein / GRIM-19 / Gene associated with retinoic and IFN-induced mortality 19 protein / NADH-ubiquinone oxidoreductase B16.6 subunit


Mass: 16881.588 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa13, Grim19 / Production host: Mus musculus (house mouse) / References: UniProt: Q9ERS2
#21: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / Complex I-MWFE / CI-MWFE / NADH-ubiquinone oxidoreductase MWFE subunit


Mass: 8149.524 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa1 / Production host: Mus musculus (house mouse) / References: UniProt: O35683
#22: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / Complex I-B9 / CI-B9 / NADH-ubiquinone oxidoreductase B9 subunit


Mass: 9338.867 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa3 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CQ91
#37: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / Complex I-B17.2 / CI-B17.2 / CIB17.2 / NADH-ubiquinone oxidoreductase subunit B17.2


Mass: 17112.416 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa12 / Production host: Mus musculus (house mouse) / References: UniProt: Q7TMF3
#38: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / Complex I-B14.5a / CI-B14.5a / NADH-ubiquinone oxidoreductase subunit B14.5a


Mass: 12464.396 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufa7 / Production host: Mus musculus (house mouse) / References: UniProt: Q9Z1P6

-
Protein , 4 types, 10 molecules ABACabac3C3N3D3OS1s1

#15: Protein
Acyl carrier protein, mitochondrial / ACP / CI-SDAP / NADH-ubiquinone oxidoreductase 9.6 kDa subunit


Mass: 17390.289 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufab1 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CR21
#42: Protein Cytochrome b


Mass: 43143.191 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Mt-Cyb / Production host: Mus musculus (house mouse) / References: UniProt: P00158
#43: Protein Cytochrome c1, heme protein, mitochondrial


Mass: 27312.188 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Cyc1 / Production host: Mus musculus (house mouse) / References: UniProt: Q9D0M3
#53: Protein NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / Complex I-75kD / CI-75kD


Mass: 78781.484 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufs1 / Production host: Mus musculus (house mouse)
References: UniProt: Q91VD9, NADH:ubiquinone reductase (H+-translocating)

-
NADH dehydrogenase [ubiquinone] 1 subunit ... , 2 types, 4 molecules C1c1C2c2

#23: Protein NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / Complex I-KFYI / CI-KFYI / NADH-ubiquinone oxidoreductase KFYI subunit


Mass: 8636.023 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufc1 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CQY9
#24: Protein NADH dehydrogenase [ubiquinone] 1 subunit C2


Mass: 14185.692 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufc2 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CQ54

-
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit ... , 11 types, 22 molecules B1b1BMbmB5b5B6b6B2b2B3b3B8b8B4b4B9b9B7b7BLbl

#26: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / Complex I-MNLL / CI-MNLL / NADH-ubiquinone oxidoreductase MNLL subunit


Mass: 6965.109 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufb1 / Production host: Mus musculus (house mouse) / References: UniProt: P0DN34
#27: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / Complex I-ESSS / CI-ESSS / NADH-ubiquinone oxidoreductase ESSS subunit / Neuronal protein 15.6 / ...Complex I-ESSS / CI-ESSS / NADH-ubiquinone oxidoreductase ESSS subunit / Neuronal protein 15.6 / Np15.6 / p15.6


Mass: 17463.727 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufb11, Np15 / Production host: Mus musculus (house mouse) / References: UniProt: O09111
#28: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / Complex I-SGDH / CI-SGDH / NADH-ubiquinone oxidoreductase SGDH subunit


Mass: 21742.197 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufb5 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CQH3
#29: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / Complex I-B17 / CI-B17 / NADH-ubiquinone oxidoreductase B17 subunit


Mass: 15408.890 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufb6, Gm137 / Production host: Mus musculus (house mouse) / References: UniProt: Q3UIU2
#30: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / Complex I-AGGG / CI-AGGG / NADH-ubiquinone oxidoreductase AGGG subunit


Mass: 11982.437 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufb2 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CPU2
#31: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / Complex I-B12 / CI-B12 / NADH-ubiquinone oxidoreductase B12 subunit


Mass: 11714.240 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufb3 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CQZ6
#32: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / Complex I-ASHI / CI-ASHI / NADH-ubiquinone oxidoreductase ASHI subunit


Mass: 21903.828 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufb8 / Production host: Mus musculus (house mouse) / References: UniProt: Q9D6J5
#33: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / Complex I-B15 / CI-B15 / NADH-ubiquinone oxidoreductase B15 subunit


Mass: 15105.287 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufb4 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CQC7
#34: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / Complex I-B22 / CI-B22 / NADH-ubiquinone oxidoreductase B22 subunit


Mass: 22020.123 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufb9 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CQJ8
#35: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / Complex I-B18 / CI-B18 / NADH-ubiquinone oxidoreductase B18 subunit


Mass: 16229.606 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufb7 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CR61
#36: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / Complex I-PDSW / CI-PDSW / NADH-ubiquinone oxidoreductase PDSW subunit


Mass: 21054.832 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufb10 / Production host: Mus musculus (house mouse) / References: UniProt: Q9DCS9

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NADH dehydrogenase [ubiquinone] flavoprotein ... , 3 types, 6 molecules V3v3V1v1V2v2

#39: Protein NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / Complex I-9kD / CI-9kD / NADH-ubiquinone oxidoreductase 9 kDa subunit


Mass: 11833.504 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufv3 / Production host: Mus musculus (house mouse) / References: UniProt: Q8BK30
#51: Protein NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / Complex I-51kD / CI-51kD / NADH-ubiquinone oxidoreductase 51 kDa subunit


Mass: 50132.305 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufv1 / Production host: Mus musculus (house mouse)
References: UniProt: Q91YT0, NADH:ubiquinone reductase (H+-translocating)
#52: Protein NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH-ubiquinone oxidoreductase 24 kDa subunit


Mass: 26948.918 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ndufv2 / Production host: Mus musculus (house mouse)
References: UniProt: Q9D6J6, NADH:ubiquinone reductase (H+-translocating)

-
Cytochrome b-c1 complex subunit ... , 9 types, 17 molecules 3A3L3B3M3E3P3F3Q3G3R3H3S3J3U3K3V3T

#40: Protein Cytochrome b-c1 complex subunit 1, mitochondrial


Mass: 49355.301 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Uqcrc1 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CZ13
#41: Protein Cytochrome b-c1 complex subunit 2, mitochondrial


Mass: 46641.773 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Uqcrc2 / Production host: Mus musculus (house mouse) / References: UniProt: Q9DB77
#44: Protein Cytochrome b-c1 complex subunit 9 / Su9 / Subunit 9 / 8 kDa subunit 9 / Complex III subunit IX / Cytochrome b-c1 complex subunit 11 / ...Su9 / Subunit 9 / 8 kDa subunit 9 / Complex III subunit IX / Cytochrome b-c1 complex subunit 11 / UQCRFS1 mitochondrial targeting sequence / UQCRFS1 MTS / Ubiquinol-cytochrome c reductase 8 kDa protein


Mass: 21524.398 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Uqcrfs1 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CR68
#45: Protein Cytochrome b-c1 complex subunit 7


Mass: 13456.336 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Uqcrb / Production host: Mus musculus (house mouse) / References: UniProt: Q9CQB4
#46: Protein Cytochrome b-c1 complex subunit 8 / Complex III subunit 8 / Complex III subunit VIII / Ubiquinol-cytochrome c reductase complex 9.5 kDa ...Complex III subunit 8 / Complex III subunit VIII / Ubiquinol-cytochrome c reductase complex 9.5 kDa protein / Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C


Mass: 9652.051 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Uqcrq / Production host: Mus musculus (house mouse) / References: UniProt: Q9CQ69
#47: Protein Cytochrome b-c1 complex subunit 6, mitochondrial / Complex III subunit 6 / Complex III subunit VIII / Cytochrome c1 non-heme 11 kDa protein / ...Complex III subunit 6 / Complex III subunit VIII / Cytochrome c1 non-heme 11 kDa protein / Mitochondrial hinge protein / Ubiquinol-cytochrome c reductase complex 11 kDa protein


Mass: 9002.015 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Uqcrh / Production host: Mus musculus (house mouse) / References: UniProt: P99028
#48: Protein Cytochrome b-c1 complex subunit 9 / Complex III subunit 9 / Complex III subunit X / Cytochrome c1 non-heme 7 kDa protein / Ubiquinol- ...Complex III subunit 9 / Complex III subunit X / Cytochrome c1 non-heme 7 kDa protein / Ubiquinol-cytochrome c reductase complex 7.2 kDa protein


Mass: 7326.330 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Uqcr10 / Production host: Mus musculus (house mouse) / References: UniProt: Q8R1I1
#49: Protein Cytochrome b-c1 complex subunit 10 / Complex III subunit 10 / Complex III subunit XI / Ubiquinol-cytochrome c reductase complex 6.4 kDa protein


Mass: 6546.627 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Uqcr11, Uqcr / Production host: Mus musculus (house mouse) / References: UniProt: Q9CPX8
#50: Protein Cytochrome b-c1 complex subunit Rieske, mitochondrial


Mass: 7900.234 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Uqcrfs1 / Production host: Mus musculus (house mouse) / References: UniProt: Q9CR68

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Non-polymers , 10 types, 36 molecules

#56: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#57: Chemical ChemComp-DGT / 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE


Mass: 507.181 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O13P3
#58: Chemical ChemComp-NDP / NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE


Mass: 745.421 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C21H30N7O17P3
#59: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#60: Chemical
ChemComp-EHZ / ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate


Mass: 584.703 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C25H49N2O9PS
#61: Chemical
ChemComp-HEM / PROTOPORPHYRIN IX CONTAINING FE / HEME


Mass: 616.487 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C34H32FeN4O4
#62: Chemical ChemComp-HEC / HEME C


Mass: 618.503 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C34H34FeN4O4
#63: Chemical ChemComp-FMN / FLAVIN MONONUCLEOTIDE / RIBOFLAVIN MONOPHOSPHATE


Mass: 456.344 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C17H21N4O9P
#64: Chemical
ChemComp-SF4 / IRON/SULFUR CLUSTER


Mass: 351.640 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: Fe4S4
#65: Chemical
ChemComp-FES / FE2/S2 (INORGANIC) CLUSTER


Mass: 175.820 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Fe2S2

-
Details

Has ligand of interestN
Has protein modificationY

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

-
Sample preparation

ComponentName: Mitochondrial Super-complex with CI dimer and CIII dimer (Super-complex XL)
Type: COMPLEX / Entity ID: #1-#9, #12, #14, #16-#41, #45-#50 / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Mus musculus (house mouse)
Buffer solutionpH: 7.7
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

MicroscopyModel: TFS GLACIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: OTHER
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 3000 nm / Nominal defocus min: 500 nm
Image recordingElectron dose: 60 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM softwareName: PHENIX / Version: 1.20.1_4487: / Category: model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 182132 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.013170083
ELECTRON MICROSCOPYf_angle_d2.158230842
ELECTRON MICROSCOPYf_dihedral_angle_d10.51722990
ELECTRON MICROSCOPYf_chiral_restr0.15225392
ELECTRON MICROSCOPYf_plane_restr0.01529290

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