EMDB-42122: Formation of I2+III2 supercomplex rescues respiratory chain defects

Basic information

Entry

Database: EMDB / ID: EMD-42122

• Title: Formation of I2+III2 supercomplex rescues respiratory chain defects
• Map data: Composite map

Sample

• Complex: Mitochondrial Super-complex with CI dimer and CIII dimer (Super-complex XL)
  • Protein or peptide: x 43 types
• Protein or peptide: x 12 types
• Ligand: x 10 types

• Keywords: Super complex XL (CI2+CIII2) / ELECTRON TRANSPORT / Translocase

Function / homology

Function:

response to D-galactosamine / Complex III assembly / Mitochondrial protein import / response to injury involved in regulation of muscle adaptation / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / Complex I biogenesis / RHOG GTPase cycle / response to mercury ion / subthalamus development / pons development / Respiratory electron transport / protein insertion into mitochondrial inner membrane / cerebellar Purkinje cell layer development / blastocyst hatching / mitochondrial respiratory chain complex III assembly / response to alkaloid / respiratory system process / pyramidal neuron development / cellular respiration / psychomotor behavior / thalamus development / Mitochondrial protein degradation / cellular response to oxygen levels / response to light intensity / mesenchymal stem cell proliferation / respiratory chain complex / mitochondrial [2Fe-2S] assembly complex / reproductive system development / iron-sulfur cluster assembly complex / mitochondrial large ribosomal subunit binding / gliogenesis / response to copper ion / response to glucagon / mesenchymal stem cell differentiation / circulatory system development / respiratory chain complex III / negative regulation of non-canonical NF-kappaB signal transduction / cardiac muscle tissue development / quinol-cytochrome-c reductase / neural precursor cell proliferation / positive regulation of mitochondrial membrane potential / [2Fe-2S] cluster assembly / adult walking behavior / oxygen sensor activity / response to hydroperoxide / quinol-cytochrome-c reductase activity / cellular response to glucocorticoid stimulus / stem cell division / mitochondrial electron transport, ubiquinol to cytochrome c / iron-sulfur cluster assembly / response to cobalamin / hypothalamus development / mitochondrial ATP synthesis coupled electron transport / midbrain development / electron transport coupled proton transport / dopamine metabolic process / NADH:ubiquinone reductase (H+-translocating) / adult behavior / positive regulation of ATP biosynthetic process / NADH dehydrogenase activity / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / positive regulation of execution phase of apoptosis / proton motive force-driven mitochondrial ATP synthesis / respiratory chain complex I / animal organ regeneration / response to hyperoxia / NADH dehydrogenase (ubiquinone) activity / neuron development / quinone binding / response to cadmium ion / cellular response to interferon-beta / ATP synthesis coupled electron transport / response to hormone / extrinsic apoptotic signaling pathway / negative regulation of reactive oxygen species biosynthetic process / cellular response to retinoic acid / tricarboxylic acid cycle / neurogenesis / Neutrophil degranulation / regulation of mitochondrial membrane potential / visual perception / muscle contraction / reactive oxygen species metabolic process / aerobic respiration / cerebellum development / hippocampus development / response to activity / respiratory electron transport chain / response to nicotine / mitochondrion organization / response to cocaine / DNA damage response, signal transduction by p53 class mediator / kidney development / fatty acid metabolic process / response to hydrogen peroxide / sensory perception of sound / monooxygenase activity / electron transport chain

Domain/homology:

Cytochrome b-c1 complex subunit 10 / Single alpha-helix domain superfamily / Ubiquinol-cytochrome C reductase complex, 6.4kD protein / NmrA-like domain / NmrA-like family / Ubiquinol-cytochrome c reductase 8kDa, N-terminal / Ubiquinol-cytochrome c reductase 8 kDa, N-terminal / Globular protein, non-globular alpha/beta subunit / Complex1_LYR-like / Cytochrome b-c1 complex, subunit 6 / Cytochrome b-c1 complex subunit 8 / UcrQ family / Cytochrome bc1 complex subunit Rieske, transmembrane domain superfamily / Cytochrome b-c1 complex subunit 7 / Cytochrome b-c1 complex subunit 7 superfamily / Ubiquinol-cytochrome C reductase complex 14kD subunit / Cytochrome b-c1 complex subunit 8 superfamily / Cytochrome b-c1 complex subunit Rieske, transmembrane domain / Ubiquinol cytochrome reductase transmembrane region / Cytochrome b-c1 complex subunit 9 / Cytochrome b-c1 complex subunit 9 superfamily / Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like / Ubiquinol-cytochrome C reductase hinge domain / Ubiquinol-cytochrome C reductase hinge domain superfamily / Ubiquinol-cytochrome C reductase hinge protein / Cytochrome c1, transmembrane anchor, C-terminal / : / Cytochrome b / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / Ubiquinol-cytochrome c reductase, iron-sulphur subunit / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / : / Cytochrome c1 / Cytochrome C1 family / : / Cytochrome b/b6, C-terminal / Cytochrome b(C-terminal)/b6/petD / Cytochrome b/b6 C-terminal region profile. / Peptidase M16, zinc-binding site / Insulinase family, zinc-binding region signature. / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / Cytochrome b/b6, C-terminal domain superfamily / Cytochrome b/b6/petB / Tim17/Tim22/Tim23/Pmp24 family / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / Rieske iron-sulphur protein, C-terminal / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH dehydrogenase 1 beta subcomplex subunit 2 / Cytochrome b/b6, N-terminal / Cytochrome b/b6-like domain superfamily / Cytochrome b/b6 N-terminal region profile. / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / : / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / Di-haem cytochrome, transmembrane / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / : / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / Rieske iron-sulphur protein / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / Zinc finger, CHCC-type / Zinc-finger domain / Peptidase M16, C-terminal / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / Peptidase M16 inactive domain / Peptidase M16, N-terminal / Insulinase (Peptidase family M16) / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family

Component:

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / Cytochrome b / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / Cytochrome b-c1 complex subunit 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH-ubiquinone oxidoreductase chain 1 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / Cytochrome b-c1 complex subunit 9 / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / Cytochrome b-c1 complex subunit 10 / NADH dehydrogenase [ubiquinone] 1 subunit C2 / Cytochrome b-c1 complex subunit 8 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / Cytochrome b-c1 complex subunit 7 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / Acyl carrier protein, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / Cytochrome b-c1 complex subunit Rieske, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / Cytochrome b-c1 complex subunit 1, mitochondrial / Cytochrome c1, heme protein, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / Cytochrome b-c1 complex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

• Biological species: Mus musculus (house mouse)
• Method: single particle reconstruction / cryo EM / Resolution: 3.7 Å
• Authors: Letts JA / Padavannil A

Funding support

United States, 1 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R35GM137929	United States

• Citation: Journal: Cell Metab / Year: 2025; Title: Formation of I+III supercomplex rescues respiratory chain defects.; Authors: Chao Liang / Abhilash Padavannil / Shan Zhang / Sheryl Beh / David R L Robinson / Jana Meisterknecht / Alfredo Cabrera-Orefice / Timothy R Koves / Chika Watanabe / Miyuki Watanabe / María Illescas / Radiance Lim / Jordan M Johnson / Shuxun Ren / Ya-Jun Wu / Dennis Kappei / Anna Maria Ghelli / Katsuhiko Funai / Hitoshi Osaka / Deborah Muoio / Cristina Ugalde / Ilka Wittig / David A Stroud / James A Letts / Lena Ho / ; Abstract: Mitochondrial electron transport chain (ETC) complexes partition between free complexes and quaternary assemblies known as supercomplexes (SCs). However, the physiological requirement for SCs and the mechanisms regulating their formation remain controversial. Here, we show that genetic perturbations in mammalian ETC complex III (CIII) biogenesis stimulate the formation of a specialized extra-large SC (SC-XL) with a structure of I+III, resolved at 3.7 Å by cryoelectron microscopy (cryo-EM). SC-XL formation increases mitochondrial cristae density, reduces CIII reactive oxygen species (ROS), and sustains normal respiration despite a 70% reduction in CIII activity, effectively rescuing CIII deficiency. Consequently, inhibiting SC-XL formation in CIII mutants using the Uqcrc1 contact site mutation leads to respiratory decompensation. Lastly, SC-XL formation promotes fatty acid oxidation (FAO) and protects against ischemic heart failure in mice. Our study uncovers an unexpected plasticity in the mammalian ETC, where structural adaptations mitigate intrinsic perturbations, and suggests that manipulating SC-XL formation is a potential therapeutic strategy for mitochondrial dysfunction.

History

Deposition	Sep 26, 2023	-
Header (metadata) release	Jan 15, 2025	-
Map release	Jan 15, 2025	-
Update	Feb 19, 2025	-
Current status	Feb 19, 2025	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_42122.map.gz / Format: CCP4 / Size: 113 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Composite map
• Voxel size: X=Y=Z: 0.88 Å

Density

Contour Level	By AUTHOR: 3.4
Minimum - Maximum	-9.364409 - 29.226735999999999
Average (Standard dev.)	-0.006920757 (±1.2912709)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order Z Y X Origin 0 0 0 Dimensions 385 271 284 Spacing 284 385 271
Cell	A: 249.92 Å / B: 338.8 Å / C: 238.48 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : Mitochondrial Super-complex with CI dimer and CIII dimer (Super-c...

• Entire: Name: Mitochondrial Super-complex with CI dimer and CIII dimer (Super-complex XL)

Components

• Complex: Mitochondrial Super-complex with CI dimer and CIII dimer (Super-complex XL)
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
  • Protein or peptide: Cytochrome b-c1 complex subunit 1, mitochondrial
  • Protein or peptide: Cytochrome b-c1 complex subunit 2, mitochondrial
  • Protein or peptide: Cytochrome b-c1 complex subunit 7
  • Protein or peptide: Cytochrome b-c1 complex subunit 8
  • Protein or peptide: Cytochrome b-c1 complex subunit 6, mitochondrial
  • Protein or peptide: Cytochrome b-c1 complex subunit 9
  • Protein or peptide: Cytochrome b-c1 complex subunit 10
  • Protein or peptide: Cytochrome b-c1 complex subunit Rieske, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
• Protein or peptide: Acyl carrier protein, mitochondrial
• Protein or peptide: Cytochrome b
• Protein or peptide: Cytochrome c1, heme protein, mitochondrial
• Protein or peptide: Cytochrome b-c1 complex subunit 9
• Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
• Protein or peptide: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
• Ligand: MAGNESIUM ION
• Ligand: 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE
• Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• Ligand: ZINC ION
• Ligand: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate
• Ligand: PROTOPORPHYRIN IX CONTAINING FE
• Ligand: HEME C
• Ligand: FLAVIN MONONUCLEOTIDE
• Ligand: IRON/SULFUR CLUSTER
• Ligand: FE2/S2 (INORGANIC) CLUSTER

Supramolecule #1: Mitochondrial Super-complex with CI dimer and CIII dimer (Super-c...

• Supramolecule: Name: Mitochondrial Super-complex with CI dimer and CIII dimer (Super-complex XL); type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#9, #12, #14, #16-#41, #45-#50
• Source (natural): Organism: Mus musculus (house mouse)

Macromolecule #1: NADH-ubiquinone oxidoreductase chain 3

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.223775 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MNLYTVIFIN ILLSLTLILV AFWLPQMNLY SEKANPYECG FDPTSSARLP FSMKFFLVAI TFLLFDLEIA LLLPLPWAIQ TIKTSTMMI MAFILVTILS LGLAYEWTQK GLEWTE

UniProtKB: NADH-ubiquinone oxidoreductase chain 3

Macromolecule #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 30.191307 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAAAAARVWC RGLLGAASVG RGAGRPSVLW QHVRRESAAA DKRPTVRPRS DVTHKQLSAF GEYVAEILPK YVQQVQVSCL DELEICIHP DGVIPTLTFL RDHTNAQFKS LADLTAVDVP TRQNRFEIVY NLLSLRFNSR IRVKTYADEL TPIDSIVSVH I AANWYERE VWDMFGVFFF NHPDLRRILT DYGFEGHPFR KDFPLTGYVE LRYDDEVKRV VAEPVELAQE FRKFDLNSPW EA FPAYRQP PESLKLEAGD KKPETK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

Macromolecule #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 52.720602 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAALRALRCL RGVGAPVLRP GSGIRLPSQP SRGARQWQPD IEWAEQFSGA VMYPSKETAH WKPPPWNDVD ILKEKAVTNM TLNFGPQHP AAHGVLRLVL ELSGEMVRKC DPHIGLLH(2MR)G TEKLIEYKTY LQALPYFDRL DYVSMMCNEQ AYSIAVE KL LNIQPPPRAQ WIRVLFGEIT RILNHIMAVT THALDIGAMT PFFWMFEERE KMFEFYERVS GARMHAAYIR PGGVHQDL P LGLLDDIYEF SKNFSLRIDE VEEMLTNNRI WRNRTVDIGV VTAEDALNYG FSGVMLRGSG IQWDLRKTQP YDVYDQVEF DVPIGSRGDC YDRYLCRVEE MRQSLRIIEQ CLNKMPPGEI KVDDAKVSPP KRAEMKTSME SLIHHFKLYT EGYQVPPGAT YTAIEAPKG EFGVYLVSDG SSRPYRCKIK APGFAHLAGL DKMSKGHMLA DVVAIIGTQD IVFGEIDR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Macromolecule #4: NADH-ubiquinone oxidoreductase chain 1

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 36.105027 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

(FME)FFINILTLL VPILIAMAFL TLVERKILGY MQLRKGPNIV GPYGILQPFA DAMKLFMKEP MRPLTTSMSL FIIAPT LSL TLALSLWVPL PMPHPLINLN LGILFILATS SLSVYSILWS GWASNSKYSL FGALRAVAQT ISYEVTMAII LLSVLLM NG SYSLQTLITT QEHMWLLLPA WPMAMMWFIS TLAETNRAPF DLTEGESELV SGFNVEYAAG PFALFFMAEY TNIILMNA L TTIIFLGPLY YINLPELYST NFMMEALLLS STFLWIRASY PRFRYDQLMH LLWKNFLPLT LALCMWHISL PIFTAGVPP YM

UniProtKB: NADH-ubiquinone oxidoreductase chain 1

Macromolecule #5: NADH-ubiquinone oxidoreductase chain 6

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 18.62809 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MNNYIFVLSS LFLVGCLGLA LKPSPIYGGL GLIVSGFVGC LMVLGFGGSF LGLMVFLIYL GGMLVVFGYT TAMATEEYPE TWGSNWLIL GFLVLGVIME VFLICVLNYY DEVGVINLDG LGDWLMYEVD DVGVMLEGGI GVAAMYSCAT WMMVVAGWSL F AGIFIIIE ITRD

UniProtKB: NADH-ubiquinone oxidoreductase chain 6

Macromolecule #6: NADH-ubiquinone oxidoreductase chain 4L

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 10.609619 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MPSTFFNLTM AFSLSLLGTL MFRSHLMSTL LCLEGMVLSL FIMTSVTSLN SNSMSSMPIP ITILVFAACE AAVGLALLVK VSNTYGTDY VQNLNLLQC

UniProtKB: NADH-ubiquinone oxidoreductase chain 4L

Macromolecule #7: NADH-ubiquinone oxidoreductase chain 5

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 68.519289 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MNIFTTSILL IFILLLSPIL ISMSNLIKHI NFPLYTTTSI KFSFIISLLP LLMFFHNNME YMITTWHWVT MNSMELKMSF KTDFFSILF TSVALFVTWS IMQFSSWYMH SDPNINRFIK YLTLFLITML ILTSANNMFQ LFIGWEGVGI MSFLLIGWWY G RTDANTAA LQAILYNRIG DIGFILAMVW FSLNMNSWEL QQIMFSNNND NLIPLMGLLI AATGKSAQFG LHPWLPSAME GP TPVSALL HSSTMVVAGI FLLVRFHPLT TNNNFILTTM LCLGALTTLF TAICALTQND IKKIIAFSTS SQLGLMMVTL GMN QPHLAF LHICTHAFFK AMLFMCSGSI IHSLADEQDI RKMGNITKIM PFTSSCLVIG SLALTGMPFL TGFYSKDLII EAIN TCNTN AWALLITLIA TSMTAMYSMR IIYFVTMTKP RFPPLISINE NDPDLMNPIK RLAFGSIFAG FVISYNIPPT SIPVL TMPW FLKTTALIIS VLGFLIALEL NNLTMKLSMN KANPYSSFST LLGFFPSIIH RITPMKSLNL SLKTSLTLLD LIWLEK TIP KSTSTLHTNM TTLTTNQKGL IKLYFMSFLI NIILIIILYS INLE

UniProtKB: NADH-ubiquinone oxidoreductase chain 5

Macromolecule #8: NADH-ubiquinone oxidoreductase chain 4

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 51.915535 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MLKIILPSLM LLPLTWLSSP KKTWTNVTSY SFLISLTSLT LLWQTDENYK NFSNMFSSDP LSTPLIILTA WLLPLMLMAS QNHLKKDNN VLQKLYISML ISLQILLIMT FSATELIMFY ILFEATLIPT LIIITRWGNQ TERLNAGIYF LFYTLIGSIP L LIALILIQ NHVGTLNLMI LSFTTHTLDA SWSNNLLWLA CMMAFLIKMP LYGVHLWLPK AHVEAPIAGS MILAAILLKL GS YGMIRIS IILDPLTKYM AYPFILLSLW GMIMTSSICL RQTDLKSLIA YSSVSHMALV IASIMIQTPW SFMGATMLMI AHG LTSSLL FCLANSNYER IHSRTMIMAR GLQMVFPLMA TWWLMASLAN LALPPSINLM GELFITMSLF SWSNFTIILM GINI IITGM YSMYMIITTQ RGKLTNHMIN LQPSHTRELT LMALHMIPLI LLTTSPKLIT GLTM

UniProtKB: NADH-ubiquinone oxidoreductase chain 4

Macromolecule #9: NADH-ubiquinone oxidoreductase chain 2

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 2 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 38.772219 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MNPITLAIIY FTIFLGPVIT MSSTNLMLMW VGLEFSLLAI IPMLINKKNP RSTEAATKYF VTQATASMII LLAIVLNYKQ LGTWMFQQQ TNGLILNMTL MALSMKLGLA PFHFWLPEVT QGIPLHMGLI LLTWQKIAPL SILIQIYPLL NSTIILMLAI T SIFMGAWG GLNQTQMRKI MAYSSIAHMG WMLAILPYNP SLTLLNLMIY IILTAPMFMA LMLNNSMTIN SISLLWNKTP AM LTMISLM LLSLGGLPPL TGFLPKWIII TELMKNNCLI MATLMAMMAL LNLFFYTRLI YSTSLTMFPT NNNSKMMTHQ TKT KPNLMF STLAIMSTMT LPLAPQLIT

UniProtKB: NADH-ubiquinone oxidoreductase chain 2

Macromolecule #10: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 40.657375 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MALRLLRLVP ASAPARGLAA GAQRVGRIHT SVHCKLRYGL LAAILGDKTT KKLHEYSRVI TVDGNICSGK NKLAKEIAQQ LGMKHYPEA GIQYSSTTTG DGRPLDIEFS GSCSLEKFYD DPKSNDGNSY RLQSWLYASR LLQYADALEH LLSTGQGVVL E RSIYSDFV FLEAMYNQGY IRKQCVDHYN EIKRLTLPEY LPPHAVIYID VPVPEVQSRI QKKGDPHEMK VTSAYLQDIE NA YKKTFLP KMSEMCEVLV YDSWEAEDPT KVVEDIEYLK YNKGPWLKQD DWTFHYLRML VQDKTEVLNY TTIPVYLPEI TIG AHQGSR IYNSFRELPG RKYAPGYNAE VGDKWIWLK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

Macromolecule #11: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 42.588129 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAAAVRFRVV RALPMSRPAI TAAATSVFCG SSHRQLHHAV IPHGKGGRSS VSGVVATVFG ATGFLGRYVV NHLGRMGSQV IIPYRCDVY DIMHLRLMGD LGQLTFLEWD ARDKDSIRKA VQHSNVVINL IGREWETRNF DFEDVFVNIP RAIAQASKEA G VERFIHVS HLNASMKSSS KSLRSKAVGE KEVRSVFPEA IIIRPSDIFG REDRFLNHFA NYRWFLAVPL VSLGFKTVKQ PV YVADVSK GIVNATKDPD AVGKTFAFTG PNRYLLFHLV KYIFGMTHRT FIPYPLPLFV YSWIGKLFGL SPFEPWTTKD KVE RIHISD VMPTDLPGLE DLGVQPTPLE LKSIEVLRRH RTYRWLSSEI EETKPAKTVN Y

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

Macromolecule #12: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 19.814725 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAAVSISVSL RQAMLGRRAM ATAAVSVCRV PSRLLSTSTW KLADNQTRDT QLITVDEKLD ITTLTGVPEE HIKTRKVRIF VPARNNMQS GVNNTKKWKM EFDTRERWEN PLMGWASTAD PLSNMVLTFS AKEDAIAFAE KNGWSYDVEE KKVPKPKSKS Y GANFSWNK RTRVSTK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

Macromolecule #13: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.041828 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAAVLTFRRL LTLPRAARGF GVQVSPSGEK ITHTGQVYDE KDYRRVRFVD RQKEVNENFA IDLIAQQPVN EVEHRIIACD GGGGALGHP KVYINLDKET KTGTCGYCGL QFKQHHH

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

Macromolecule #14: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 10.932675 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAAAAASRAV GAKLGLREIR VHLCQRSPGS QGVRDFIVQR YVELKKAHPN LPILIRECSE VQPKLWARYA FGQEKTVSLN NLSADEVTR AMQNVLSGKA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

Macromolecule #15: Acyl carrier protein, mitochondrial

• Macromolecule: Name: Acyl carrier protein, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.390289 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MASRVLCACV RRLPAAFAPL PRLPTLALAR PLSTTLCPEG IRRRPGALQS ALALAQVPGT VTHLCRQYSD APPLTLDGIK DRVLYVLKL YDKIDPEKLS VNSHFMKDLG LDSLDQVEII MAMEDEFGFE IPDIDAEKLM CPQEIVDYIA DKKDVYE

UniProtKB: Acyl carrier protein, mitochondrial

Macromolecule #16: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.380719 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAGLLKKTTG LVGLAVCDTP HERLTILYTK TLDILKHFPK HAAYRKYTEQ ITNEKLDMVK AEPDVKKLEA LLQGGEVEEV ILQAEKELS LARKMLKWKP WEPLVEEPPA NQWKWPI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

Macromolecule #17: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6; type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.311858 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAAAATGLRQ AAAAAASTSV KPIFSRDLNE AKRRVRELYR AWYREVPNTV HLMQLDITVK QGRDKVREMF MKNAHVTDPR VVDLLVIKG KMELQETIKV WKQRTHVMRF FHETETPRPK DFLSKFYMGH DP

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

Macromolecule #18: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 20.025127 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MPGIVELPTL EELKVEEVKV SSAVLKAAAH HYGAQCDKTN KEFMLCRWEE KDPRRCLKEG KLVNGCALNF FRQIKSHCAE PFTEYWTCL DYSNMQLFRH CRQQQAKFDQ CVLDKLGWVR PDLGQLSKVT KVKTDRPLPE NPYHSRARPE PNPVIEGDLK P AKHGTRFF FWTV

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

Macromolecule #19: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11; type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 14.99922 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

AMVKRFFESY HEVPDGTQCH RKTYITTALG GICGIIGSAY SVSLNPADST LEAVARVGRY TFTAAAIGAM FGLTTCVSAQ VREKPDDPL NYFIGGCAGG LTLGARTHSY GTAAMGCVYM GTAAALFKIG KLEGWELFPT PKV

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

Macromolecule #20: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.881588 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAASKVKQDM PPPGGYGPID YKRNLPRRGL SGYSMFAVGI GALIFGYWRM MRWNQERRRL LIEDLEARIA LMPLFQAEKD RRTLQILRE NLEEEAIIMK DVPNWKVGES VFHTTRWVPP LIGEMYGLRT KEEMSNANFG FTWYT

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Macromolecule #21: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 8.149524 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MWFEILPGLA IMGVCLVIPG VSTAYIHKFT NGGKEKRVAR VQYQWYLMER DRRISGVNRY YVSKGLENID

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

Macromolecule #22: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3; type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 9.338867 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAGRISAFLK NAWAKEPVLV VSFSVWGLAI IMPMISPYTK YASMINKATP YNYPVPVRDD GNMPDVPSHP QDPLGPSLDW LKNL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

Macromolecule #23: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial; type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 8.636023 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAPSVVLRSF SRLLAPARLP SCSSTRSKFY VREPVNAKPN WLAVGLSVGA SVFMWIYLIQ THNEDVLEYK RRNGLE

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

Macromolecule #24: NADH dehydrogenase [ubiquinone] 1 subunit C2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 subunit C2 / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 14.185692 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MMNGRPGHEP LKFLPDEARS LPPPKLNDPR LVYMGLLGYC TGLMDNMLRM RPVMRAGLHR QLLFVTSFVF AGYFYLKRQN YLYAVKDHD MFGYIKLHPE DFPEKEKKTY AEILEPFHPV R

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C2

Macromolecule #25: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 12.675772 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MPFLDIQKKL GISLDRHFMF LSAEQPYKNA ARCHAFEKEW IECAHGIGGT RAKKECKIEF DDFEECLLRY KTMRRMHDIK KQREKLMKE GKYTPPPHHS GREEPRP

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

Macromolecule #26: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1; type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 6.965109 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MTLFQLLREH WVHILVPAGF VFGCYLDRKD DEKLTAFRNK SMLFQRELRP NEEVTWK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

Macromolecule #27: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial; type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.463727 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAARLLSLYG RCLSAAGAMR GLPAARVRWE SSRAVIAPSG VEKKRQREPT MQWQEDPEPE DENVYAKNPD FHGYDSDPVV DVWNMRAVF FFGFSIVLVF GTTFVAYVPD YRMQEWARRE AERLVKYREV NGLPIMESNY FDPSKIQLPE DD

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

Macromolecule #28: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial; type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.742197 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAAMSLLQRA SVSALTALSC RRAGPRLGVG SFLTRSFPKT VAPVRHSGDH GKRLFVVKPS LYYDARFLRL MKFYLMLTGI PVIIGITLV NIFIGEAELA EIPEGYIPEH WEYYKHPISR WIARNFYDGP EKNYEKTLAI LQIESEKAEL RLKEQEVRRL M RARGDGPW YQFPTPEKEF IDHSPKATPD N

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

Macromolecule #29: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6; type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.40889 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

SGYTPDEKLR LQQLRELRRR WLKDQELSPR EPVLPPRRMW PLERFWDNFL RDGAVWKNMV FKAYRSSLFA VSHVLIPMWF VHYYVKYHM ATKPYTIVSS KPRIFPGDTI LETGEVIPPM RDFPDQHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

Macromolecule #30: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial; type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 11.982437 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MSALTRLVPF GRVGGRLLRG CRARAAGDSG VRHAGGGVHI QPRYREFPQL TRSQVIQGEF LSSLMWFWIL WRFWHDSDAV LGHFSYPDP SQWTDEELGI PPDDED

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

Macromolecule #31: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3; type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 11.71424 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAAGHGHEHG HEHGHGHGKM ELPDYRQWKI EGTPLETVQK KLAARGLRDP WARNEAWRYM GGFAGNITFP SVILKGFKWG FAAFVVALG AEYFLDSQNG DKKHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

Macromolecule #32: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.903828 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAAARAAALG VRWLQRTTRG VVPLEARRAF HMTKDMLPGS YPRTPEERAA AAKKYNMRVE DYEPYPDDGM GYGDYPMLPN RSQHERDPW YQWDHSELRM NWGEPIHWDL DMYIRNRVDT SPTPVSWDVM CKHLFGFVAF MVFMFWVGHV FPSYQPVGPK Q YPYNNLYL ERGGDPTKEP EPVVHYDI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

Macromolecule #33: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.105287 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MSGSKYKPAP LATLPSTLDP AEYDVSPETR RAQVERLSIR ARLKREYLLQ YNDPKRVSHI EDPALIRWTY ARSANIYPNF RPTPKNSLL GAVAGFGPLI FWYYVFKTDR DRKERLIQEG KLDRKFNISY

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Macromolecule #34: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 22.020123 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAFCAPPAYL THQQKVLRLY KRALRHLESW CIHRDKYRYF ACLMRARFEE HKNEKDMMRA TQLLREAEEE FWQNQHPQPY IFPDSPGGT SFERYECYKV PEWCLDYWHP SEKAMYPDYF SKREQWKKLR MESWDREVKQ LQEETSPDGI MTEALPPARR E GDLPPLWW HIVTRPRERP T

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

Macromolecule #35: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.229606 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

GAHLTRRYLW DASVEPDPEK IPSFPPDLGF PERKERVMVA TQQEMMDAQL TLQQRDYCAH YLIRLLKCKR DSFPNFLACK HEQHDWDYC EHLDYVKRMK EFERERRLLQ RKKRRALKEA RVAQGQGEGE VGPEVAL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Macromolecule #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; type: protein_or_peptide / ID: 36 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.054832 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MPDSWDKDVY PEPPSRTPAP SPQTSLPNPI TYLTKAYDLV VDWPVTLVRE FIERQHAKNR TYYYHRQYRR VPDITECKEG DVLCIYEAE MQWRRDFKVD QEIMNIIQER LKACQQREGE NYQQNCAKEL EQFTKVTKAY QDRYLDLGAY YSARKCLAKQ K QRMLEERK AARQEAAA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

Macromolecule #37: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12; type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.112416 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MELVEVLKRG VQQVTGHGGL RGLLRVFFRA NDIRIGTLVG EDKYGNKYYE DNKQFFGRHR WVIYTTEMNG KNTFWDVDGS MVPPEWHRW LHCMTDDPPT TNPPTARKFI WTNHKFNVSA TPEQYVPYST TRKKIHEWVP PSTPYK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

Macromolecule #38: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7; type: protein_or_peptide / ID: 38 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 12.464396 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

ASATRVIQKL RNWASGQDLQ AKLQLRYQEI AKRTQPPPKL PVGPSHKLSN NYYCTRDGRR EVVPPSIIMS SQKALVSGKA AESSAMAAT EKKAVTPAPP MKRWELSKDQ PYL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

Macromolecule #39: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial; type: protein_or_peptide / ID: 39 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 11.833504 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAVSLLLRGG RIRALKAVLL EARVFPGELV SVVRLSTESE KSAKEKELHP KTQSVLKEPE PTDTTTYKNL QHHDYNTYTF LDLNLDLSK FRLPQPSSGR ESPRH

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

Macromolecule #40: Cytochrome b-c1 complex subunit 1, mitochondrial

• Macromolecule: Name: Cytochrome b-c1 complex subunit 1, mitochondrial / type: protein_or_peptide / ID: 40 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 49.355301 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

TATFAQALQS VPETQVSILD NGLRVASEQS SHATCTVGVW IDAGSRYETE KNNGAGYFLE HLAFKGTKNR PGNALEKEVE SIGAHLNAY STREHTAYLI KALSKDLPKV VELLADIVQN SSLEDSQIEK ERDVILREMQ ENDASMQNVV FDYLHATAFQ G TPLAQAVE GPSENVRRLS RTDLTDYLNR HYKAPRMVLA AAGGVEHQQL LDLAQKHLSS VSRVYEEDAV PGLTPCRFTG SE IRHRDDA LPLAHVAIAV EGPGWANPDN VTLQVANAII GHYDCTYGGG VHLSSPLASV AVANKLCQSF QTFNISYSDT GLL GAHFVC DAMSIDDMVF FLQGQWMRLC TSATESEVTR GKNILRNALV SHLDGTTPVC EDIGRSLLTY GRRIPLAEWE SRIQ EVDAQ MLRDICSKYF YDQCPAVAGY GPIEQLPDYN RIRSGMFWLR F

UniProtKB: Cytochrome b-c1 complex subunit 1, mitochondrial

Macromolecule #41: Cytochrome b-c1 complex subunit 2, mitochondrial

• Macromolecule: Name: Cytochrome b-c1 complex subunit 2, mitochondrial / type: protein_or_peptide / ID: 41 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 46.641773 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

SLKVAPKVKT SAAPGGVPLQ PQDLEFTKLP NGLVIASLEN YAPLSRIGLF VKAGSRYEDS NNLGTSHLLR LASSLTTKGA SSFKITRGI EAVGGKLSVT ATRENMAYTV EGIRSDIEIL MEFLLNVTTA PEFRRWEVAA LRSQLKIDKA VAFQNSQTRI I ENLHDVAY KNALANPLYC PDYRMGKITS EELHYFVQNH FTSARMALVG LGVSHSVLKQ VAEQFLNMRG GLGLAGAKAK YR GGEIREQ NGDNLVHAAI VAESAAIGNA EANAFSVLQH LLGAGPHIKR GNNTTSLLSQ SVAKGSHQPF DVSAFNASYS DSG LFGIYT ISQAAAAGEV INAAYNQVKA VAQGNLSSAD VQAAKNKLKA GYLMSVETSE GFLSEIGSQA LAAGSYMPPS TVLQ QIDSV ADADVVKAAK KFVSGKKSMA ASGNLGHTPF LDEL

UniProtKB: Cytochrome b-c1 complex subunit 2, mitochondrial

Macromolecule #42: Cytochrome b

• Macromolecule: Name: Cytochrome b / type: protein_or_peptide / ID: 42 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 43.143191 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MTNMRKTHPL FKIINHSFID LPAPSNISSW WNFGSLLGVC LMVQIITGLF LAMHYTSDTM TAFSSVTHIC RDVNYGWLIR YMHANGASM FFICLFLHVG RGLYYGSYTF METWNIGVLL LFAVMATAFM GYVLPWGQMS FWGATVITNL LSAIPYIGTT L VEWIWGGF SVDKATLTRF FAFHFILPFI IAALAIVHLL FLHETGSNNP TGLNSDADKI PFHPYYTIKD ILGILIMFLI LM TLVLFFP DMLGDPDNYM PANPLNTPPH IKPEWYFLFA YAILRSIPNK LGGVLALILS ILILALMPFL HTSKQRSLMF RPI TQILYW ILVANLLILT WIGGQPVEHP FIIIGQLASI SYFSIILILM PISGIIEDKM LKLY

UniProtKB: Cytochrome b

Macromolecule #43: Cytochrome c1, heme protein, mitochondrial

• Macromolecule: Name: Cytochrome c1, heme protein, mitochondrial / type: protein_or_peptide / ID: 43 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 27.312188 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

SDLELHPPSY PWSHRGLLSS LDHTSIRRGF QVYKQVCSSC HSMDYVAYRH LVGVCYTEEE AKALAEEVEV QDGPNDDGEM FMRPGKLSD YFPKPYPNPE AARAANNGAL PPDLSYIVRA RHGGEDYVFS LLTGYCEPPT GVSLREGLYF NPYFPGQAIG M APPIYTEV LEYDDGTPAT MSQVAKDVAT FLRWASEPEH DHRKRMGLKM LLMMGLLLPL TYAMKRHKWS VLKSRKLAYR PP K

UniProtKB: Cytochrome c1, heme protein, mitochondrial

Macromolecule #44: Cytochrome b-c1 complex subunit 9

• Macromolecule: Name: Cytochrome b-c1 complex subunit 9 / type: protein_or_peptide / ID: 44 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.524398 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

SHTDVKVPDF SDYRRAEVLD STKSSKESSE ARKGFSYLVT ATTTVGVAYA AKNVVSQFVS SMSASADVLA MSKIEIKLSD IPEGKNMAF KWRGKPLFVR HRTKKEIDQE AAVEVSQLRD PQHDLDRVKK PEWVILIGVC THLGCVPIAN AGDFGGYYCP C HGSHYDAS GRIRKGPAPL NLEVPAYEFT SDDVVVVG

UniProtKB: Cytochrome b-c1 complex subunit Rieske, mitochondrial

Macromolecule #45: Cytochrome b-c1 complex subunit 7

• Macromolecule: Name: Cytochrome b-c1 complex subunit 7 / type: protein_or_peptide / ID: 45 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.456336 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

AGRSAVSASS KWLDGFRKWY YNAAGFNKLG LMRDDTLHET EDVKEAIRRL PEDLYNDRMF RIKRALDLTM RHQILPKDQW TKYEEDKFY LEPYLKEVIR ERKEREEWAK K

UniProtKB: Cytochrome b-c1 complex subunit 7

Macromolecule #46: Cytochrome b-c1 complex subunit 8

• Macromolecule: Name: Cytochrome b-c1 complex subunit 8 / type: protein_or_peptide / ID: 46 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 9.652051 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

GREFGNLARI RHVISYSLSP FEQRAFPSYF SKGIPNVLRR TRERILRVAP PFVVVYLIYT WGNQEFEQSK RKNPAMYEND K

UniProtKB: Cytochrome b-c1 complex subunit 8

Macromolecule #47: Cytochrome b-c1 complex subunit 6, mitochondrial

• Macromolecule: Name: Cytochrome b-c1 complex subunit 6, mitochondrial / type: protein_or_peptide / ID: 47 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 9.002015 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

GDPKEEEEEE LVDPLTTVRE HCEQLEKCVK ARERLELCDN RVSSRSQTEE DCTEELFDFL HARDHCVAHK LFKNLK

UniProtKB: Cytochrome b-c1 complex subunit 6, mitochondrial

Macromolecule #48: Cytochrome b-c1 complex subunit 9

• Macromolecule: Name: Cytochrome b-c1 complex subunit 9 / type: protein_or_peptide / ID: 48 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 7.32633 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

SSPTIPSRLY SLLFRRTSTF ALTIAVGALF FERAFDQGAD AIYEHINEGK LWKHIKHKYE NKE

UniProtKB: Cytochrome b-c1 complex subunit 9

Macromolecule #49: Cytochrome b-c1 complex subunit 10

• Macromolecule: Name: Cytochrome b-c1 complex subunit 10 / type: protein_or_peptide / ID: 49 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 6.546627 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MLSRFLGPRY RELARNWIPT AGMWGTVGAV GLVWATDWRL ILDWVPYING KFKKDD

UniProtKB: Cytochrome b-c1 complex subunit 10

Macromolecule #50: Cytochrome b-c1 complex subunit Rieske, mitochondrial

• Macromolecule: Name: Cytochrome b-c1 complex subunit Rieske, mitochondrial / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 7.900234 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MLSVAARSGP FAPVLSATSR GVAGALRPLL QGAVPAASEP PVLDVKRPFL CRESLSGQAA ARPLVATVGL NVPASVRF

UniProtKB: Cytochrome b-c1 complex subunit Rieske, mitochondrial

Macromolecule #51: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; type: protein_or_peptide / ID: 51 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 50.132305 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MLAARHFLGG LVPVRVSVRF SSGTTAPKKT SFGSLKDEDR IFTNLYGRHD WRLKGALRRG DWYKTKEILL KGPDWILGEM KTSGLRGRG GAGFPTGLKW SFMNKPSDGR PKYLVVNADE GEPGTCKDRE IMRHDPHKLV EGCLVGGRAM GARAAYIYIR G EFYNEASN LQVAIREAYE AGLIGKNACG SDYDFDVFVV RGAGAYICGE ETALIESIEG KQGKPRLKPP FPADVGVFGC PT TVANVET VAVSPTICRR GGTWFAGFGR ERNSGTKLFN ISGHVNHPCT VEEEMSVPLK ELIEKHAGGV TGGWDNLLAV IPG GSSTPL IPKSVCETVL MDFDALVQAQ TGLGTAAVIV MDRSTDIVKA IARLIEFYKH ESCGQCTPCR EGVDWMNKVM ARFV KGDAR PAEIDSLWEI SKQIEGHTIC ALGDGAAWPV QGLIRHFRPE LEDRMQRFAQ QH

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Macromolecule #52: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; type: protein_or_peptide / ID: 52 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 26.948918 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MFSLALRARA TGLAAQWGRH ARNLHKTAVH NGAGGALFVH RDTPENNPDT PFDFTPENYK RIEAIVKNYP EGHQAAAVLP VLDLAQRQN GWLPISAMNK VAEVLQVPPM RVYEVATFYT MYNRKPVGKY HIQVCTTTPC MLRDSDSILE TLQRKLGIKV G ETTPDKLF TLIEVECLGA CVNAPMVQIN DNYYEDLTPK DIEEIIDELK AGKVPKPGPR SGRFCCEPAG GLTSLTEPPK GP GFGV

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Macromolecule #53: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

• Macromolecule: Name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; type: protein_or_peptide / ID: 53 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 78.781484 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MLRIPIKRAL IGLSNSPKGY VRTTGTAASN LIEVFVDGQS VMVEPGTTVL QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVV AACAMPVMKG WNILTNSEKS KKAREGVMEF LLANHPLDCP ICDQGGECDL QDQSMMFGSD RSRFLEGKRA V EDKNIGPL VKTIMTRCIQ CTRCIRFASE IAGVDDLGTT GRGNDMQVGT YIEKMFMSEL SGNVIDICPV GALTSKPYAF TA RPWETRK TESIDVMDAV GSNIVVSTRT GEVMRILPRM HEDINEEWIS DKTRFAYDGL KRQRLTEPMV RNEKGLLTYT SWE DALSRV AGMLQNFEGN AVAAIAGGLV DAEALVALKD LLNKVDSDNL CTEEIFPTEG AGTDLRSNYL LNTTIAGVEE ADVV LLVGT NPRFEAPLFN ARIRKSWLHN DLKVALIGSP VDLTYRYDHL GDSPKILQDI ASGRHSFCEV LKDAKKPMVV LGSSA LQRD DGAAILVAVS NMVQKIRVTT GVAAEWKVMN ILHRIASQVA ALDLGYKPGV EAIRKNPPKM LFLLGADGGC ITRQDL PKD CFIVYQGHHG DVGAPMADVI LPGAAYTEKS ATYVNTEGRA QQTKVAVTPP GLAREDWKII RALSEIAGIT LPYDTLD QV RNRLEEVSPN LVRYDDIEET NYFQQASELA KLVNQEVLAD PLVPPQLTIK DFYMTDSISR ASQTMAKCVK AVTE

UniProtKB: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Macromolecule #54: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; type: protein_or_peptide / ID: 54 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 24.715912 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MAALAAPGLL SVRILGLRTA QVQLRRVHQS VATEGPSPSP SPSLSSTQSA VSKAGAGAVV PKLSHLPRSR AEYVVTKLDD LINWARRSS LWPMTFGLAC CAVEMMHMAA PRYDMDRFGV VFRASPRQAD VMIVAGTLTN KMAPALRKVY DQMPEPRYVV S MGSCANGG GYYHYSYSVV RGCDRIVPVD IYVPGCPPTA EALLYGILQL QRKIKREQKL KIWYRR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

Macromolecule #55: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; type: protein_or_peptide / ID: 55 / Number of copies: 2 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 24.068355 KDa
• Recombinant expression: Organism: Mus musculus (house mouse)

Sequence

String:

MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM KSATDNAARI LMWTELIRGL GMTLSYLFRE PATINYPFE KGPLSPRFRG EHALRRYPSG EERCIACKLC EAICPAQAIT IEAEPRADGS RRTTRYDIDM TKCIYCGFCQ E ACPVDAIV EGPNFEFSTE THEELLYNKE KLLNNGDKWE AEIAANIQAD YLYR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Macromolecule #56: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 56 / Number of copies: 2 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #57: 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 57 / Number of copies: 2 / Formula: DGT
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-DGT:
2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE

Macromolecule #58: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• Macromolecule: Name: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE; type: ligand / ID: 58 / Number of copies: 2 / Formula: NDP
• Molecular weight: Theoretical: 745.421 Da

Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Macromolecule #59: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 59 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #60: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butan...

• Macromolecule: Name: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate; type: ligand / ID: 60 / Number of copies: 4 / Formula: EHZ
• Molecular weight: Theoretical: 584.703 Da

Chemical component information

ChemComp-EHZ:
~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate

Macromolecule #61: PROTOPORPHYRIN IX CONTAINING FE

• Macromolecule: Name: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 61 / Number of copies: 4 / Formula: HEM
• Molecular weight: Theoretical: 616.487 Da

Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

Macromolecule #62: HEME C

• Macromolecule: Name: HEME C / type: ligand / ID: 62 / Number of copies: 2 / Formula: HEC
• Molecular weight: Theoretical: 618.503 Da

Chemical component information

ChemComp-HEC:
HEME C

Macromolecule #63: FLAVIN MONONUCLEOTIDE

• Macromolecule: Name: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 63 / Number of copies: 2 / Formula: FMN
• Molecular weight: Theoretical: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

Macromolecule #64: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 64 / Number of copies: 12 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Macromolecule #65: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 65 / Number of copies: 4 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.7
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: TFS GLACIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm

Image processing

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

8om1

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 182132
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD