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Yorodumi- PDB-8tx1: Characterization of the Chlamydomonas Flagellar Mastigoneme Filam... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8tx1 | ||||||
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Title | Characterization of the Chlamydomonas Flagellar Mastigoneme Filament Structure at 3.6A | ||||||
Components | Mastigoneme-like protein | ||||||
Keywords | STRUCTURAL PROTEIN / Hair-like structures attached to the protistan flagella | ||||||
Function / homology | Tyrosine-protein kinase ephrin type A/B receptor-like / Tyrosine-protein kinase ephrin type A/B receptor-like / Putative ephrin-receptor like / Growth factor receptor cysteine-rich domain superfamily / Mastigoneme-like protein Function and homology information | ||||||
Biological species | Chlamydomonas reinhardtii (plant) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.62 Å | ||||||
Authors | Yue, W. / Kai, Z. | ||||||
Funding support | United States, 1items
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Citation | Journal: J Cell Biol / Year: 2023 Title: Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes. Authors: Yue Wang / Jun Yang / Fangheng Hu / Yuchen Yang / Kaiyao Huang / Kai Zhang / Abstract: Mastigonemes are thread-like structures adorning the flagella of protists. In Chlamydomonas reinhardtii, filamentous mastigonemes find their roots in the flagella's distal region, associated with the ...Mastigonemes are thread-like structures adorning the flagella of protists. In Chlamydomonas reinhardtii, filamentous mastigonemes find their roots in the flagella's distal region, associated with the channel protein PKD2, implying their potential contribution to external signal sensing and flagellar motility control. Here, we present the single-particle cryo-electron microscopy structure of the mastigoneme at 3.4 Å. The filament unit, MST1, consists of nine immunoglobulin-like domains and six Sushi domains, trailed by an elastic polyproline-II helix. Our structure demonstrates that MST1 subunits are periodically assembled to form a centrosymmetric, non-polar filament. Intriguingly, numerous clustered disulfide bonds within a ladder-like spiral configuration underscore structural resilience. While defects in the mastigoneme structure did not noticeably affect general attributes of cell swimming, they did impact specific swimming properties, particularly under varied environmental conditions such as redox shifts and heightened viscosity. Our findings illuminate the potential role of mastigonemes in flagellar motility and suggest their involvement in diverse environmental responses. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8tx1.cif.gz | 314.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8tx1.ent.gz | 252.4 KB | Display | PDB format |
PDBx/mmJSON format | 8tx1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8tx1_validation.pdf.gz | 1.4 MB | Display | wwPDB validaton report |
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Full document | 8tx1_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 8tx1_validation.xml.gz | 66.8 KB | Display | |
Data in CIF | 8tx1_validation.cif.gz | 100.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tx/8tx1 ftp://data.pdbj.org/pub/pdb/validation_reports/tx/8tx1 | HTTPS FTP |
-Related structure data
Related structure data | 41674MC 8txbC 8txcC 8xtc M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 205404.203 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cw15 / References: UniProt: Q8LRM7 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: mastigoneme / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: all / Source: NATURAL |
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Source (natural) | Organism: Chlamydomonas reinhardtii (plant) / Strain: cw15 |
Buffer solution | pH: 7.4 Details: 30 mM HEPES, 5 mM MgSO4, 1 mM DTT, 0.5 mM EGTA, 25 mM KCl, 1 mM PMSF, pH 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Specimen support | Grid material: GOLD / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R2/1 |
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 281 K |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 39.2 e/Å2 / Film or detector model: GATAN K2 QUANTUM (4k x 4k) |
-Processing
CTF correction | Type: NONE | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 3.62 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 98875 / Symmetry type: POINT | ||||||||||||||||||||||||
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