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Open data
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Basic information
Entry | Database: PDB / ID: 8twg | ||||||
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Title | Crystal structure of tetracycline destructase Tet(56-2) | ||||||
![]() | 6-hydroxynicotinate 3-monooxygenase | ||||||
![]() | OXIDOREDUCTASE | ||||||
Function / homology | 6-hydroxynicotinate 3-monooxygenase / 6-hydroxynicotinate 3-monooxygenase activity / FAD-binding domain / FAD binding domain / FAD binding / FAD/NAD(P)-binding domain superfamily / FLAVIN-ADENINE DINUCLEOTIDE / 6-hydroxynicotinate 3-monooxygenase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kumar, H. / Tang, W.K. / Tolia, N. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Sequence-structure-function characterization of the emerging tetracycline destructase family of antibiotic resistance enzymes. Authors: Blake, K.S. / Kumar, H. / Loganathan, A. / Williford, E.E. / Diorio-Toth, L. / Xue, Y.P. / Tang, W.K. / Campbell, T.P. / Chong, D.D. / Angtuaco, S. / Wencewicz, T.A. / Tolia, N.H. / Dantas, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 200.7 KB | Display | ![]() |
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PDB format | ![]() | 128 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 719.4 KB | Display | ![]() |
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Full document | ![]() | 720.5 KB | Display | |
Data in XML | ![]() | 18 KB | Display | |
Data in CIF | ![]() | 26.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8twfC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 45602.922 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-FAD / |
#3: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.03 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / Details: 0.15 M Magnesium formate, 15% Polyethylene glycol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 1, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→19.97 Å / Num. obs: 40189 / % possible obs: 99.82 % / Redundancy: 6.51 % / Biso Wilson estimate: 22.73 Å2 / CC1/2: 0.99 / Net I/σ(I): 17.78 |
Reflection shell | Resolution: 1.8→1.86 Å / Num. unique obs: 3916 / CC1/2: 0.93 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.86 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→19.97 Å
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Refine LS restraints |
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LS refinement shell |
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